Research progress on fluorescence analysis of antitumor Chinese medicine components

This paper classifies the anti-tumor traditional Chinese medicine's fluorescence analysis and finds that its research content mainly includes two aspects:the application of fluorescence analysis in the determination of the effective component content of anti-tumor traditional Chinese medicine;the study of the combined use of fluorescence imaging technology and the drug.According to literature analysis,it is found that although the research on the fluorescence analysis of anti-tumor Chinese medicine components is in a good state of development,it still needs to explore further in the identification of unknown components and deeper research on known components.Therefore,it can provide a certain basis for clinical guidance of tumor patients.

Comprehensive characterization of yam tuber nutrition and medicinal quality of Dioscorea opposita and D.alata from different geographic groups in China

China is an important domestication center of yams,and two main yam species of Dioscorea opposita and D.alata are commonly cultivated in China.However,the differences of nutritional and medicinal characteristics between the two species and their subgroups remain unclear,which would greatly affect the resource conservation and commercial utilization of yams.In this study,typical yam resources including the species of D.opposita(wild and cultivated Ruichang yam from southern China,and Tiegun yam from northern China)and two landraces of D.alata(Longyan yam and Anyuan yam from southern China)were selected as materials.Nutritional traits and medicinal characteristics were determined and analyzed respectively.The results showed that there was no significant differences in the content of most nutrients between D.opposita and D.alata,but most cultivated Ruichang yam of D.opposita showed higher levels of starch,soluble sugar,sucrose,and ascorbate in tuber than that in yam from D.alata.Moreover,an UPLC-MS method was developed for identification and determination of medicinal characteristics in the two species.The results showed that allantoin can be detected in all selected samples.Cultivated Ruichang yam of D.opposita possessed the highest allantoin content among the tested materials,and was significantly different with that in Tiegun yam and D.alata.Dioscin was not detected in D.alata.Overall,there was little difference in nutritional composition between D.opposita and D.alata,but the medicinal quality of D.opposita was better than that of D.alata.Due to the outstanding comprehensive quality,the local variety of cultivated Ruichang yam can be further developed and utilized.

Quantification of eight components in Paeonia lactiflora and Glycyrrhiza decoction using UHPLC-MS/MS

Hao Jiang;Yushu Guo;Chao Shi;Department of Pharmacy,Medical Supplies Center of PLA General Hospital;This study aimed to utilize ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)to analyze and quantify eight therapeutic components in Paeonia lactiflora and Glycyrrhiza decoction.The goal was to establish an accurate,efficient,and applicable method for determining the composition of this formula,providing a reference for its clinical application.Methanol was employed to extract the therapeutic components from Paeonia lactiflora and Glycyrrhiza decoction,forming the test sample solution.UHPLC-MS/MS technology was applied to separate and analyze the test samples.The results indicated that upon analysis,the eight reference standards exhibited excellent linear relationships,and the test samples remained stable within 24 h.The content of paeoniflorin,paeonolide,glycyrrhizic acid A,quercetin,chebulic acid,liquiritin,naringenin,and glycyrrhetic acid was determined to be 3.530±0.124,0.139±0.006,0.245±0.008,0.779±0.026,0.433±0.021,0.388±0.018,0.617±0.013,and 0.081±0.005 mg/m L,respectively.This study established that UHPLC-MS/MS analysis demonstrated high efficiency and broad applicability,reflecting the chemical composition characteristics of Paeonia lactiflora and Glycyrrhiza decoction.This research provided a quantitative analysis method for the eight therapeutic components in Paeonia lactiflora and Glycyrrhiza decoction,applicable to its clinical use and quality control.