Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition

Objective: To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the Plackett-Burman design (PBD) and the central composite design (CCD) was employed to investigate the individual crucial component of the medium that significantly affected the enzyme yield. Results: Firstly, NaNO3, yeast extract, urea, Na2CO3, MgSO4, peptone and (NH4)2SO4 were screened as the significant factors positively affecting the xylanase production by PBD. Secondly, by valuating the nitrogen sources effect, urea was proved to be the most effective and economic nitrogen source for xylanase production and used for further optimization. Finally, the CCD and response surface methodology (RSM) were applied to determine the optimal concentration of each sig-nificant variable, which included urea, Na2CO3 and MgSO4. Subsequently a second-order polynomial was determined by multiple regression analysis. The optimum values of the critical components for maximum xylanase production were obtained as follows: x1 (urea)=0.163 (41.63 g/L), x2 (Na2CO3)=?1.68 (2.64 g/L), x3 (MgSO4)=1.338 (10.68 g/L) and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized condition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. Conclusion: By using PBD and CCD, we obtained the optimal composition for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization.

Optimization of Culture Conditions and Medium Composition for the Marine Algicidal Bacterium Alteromonas sp. DH46 by Uniform Design

Harmful algal blooms(HABs) have led to extensive ecological and environmental issues and huge economic losses.Various HAB control techniques have been developed,and biological methods have been paid more attention.Algicidal bacteria is a general designation for bacteria which inhibit algal growth in a direct or indirect manner,and kill or damage the algal cells.A metabolite which is strongly toxic to the dinoflagellate Alexandrium tamarense was produced by strain DH46 of the alga-lysing bacterium Alteromonas sp.The culture conditions were optimized using a single-factor test method.Factors including carbon source,nitrogen source,temperature,initial pH value,rotational speed and salinity were studied.The results showed that the cultivation of the bacteria at 28℃ and 180 r min-1with initial pH 7 and 30 salt contcentration favored both the cell growth and the lysing effect of strain DH46.The optimal medium composition for strain DH46 was determined by means of uniform design experimentation,and the most important components influencing the cell density were tryptone,yeast extract,soluble starch,NaNO3 and MgSO4.When the following culture medium was used(tryptone 14.0g,yeast extract 1.63g,soluble starch 5.0 g,NaNO3 1.6 g,MgSO4 2.3 g in 1L),the largest bacterial dry weight(7.36 g L-1) was obtained,which was an enhancement of 107% compared to the initial medium;and the algal lysis rate was as high as 98.4% which increased nearly 10% after optimization.

Statistical optimization of medium composition and culture condition for the production of recombinant anti-lipopolysaccharide factor of Eriocheir sinensis in Escherichia coli

Anti-lipopolysaccharide factors （ALFs） are important antimicrobial peptides that are isolated from some aquatic species. In a previous study, we isolated ALF genes from Chinese mitten crab, Eriocheir sinensis. In this study, we optimized the production of a recombinant ALF by expressing E. sinensis ALF genes in Eseherichia coli maintained in shake-flasks. In particular, we focused on optimization of both the medium composition and the culture condition. Various medium components were analyzed by the Plackett-Burman design, and two significant screened factors, （NH4）2SO4 and KH2PO4, were further optimized via the central composite design （CCD）. Based on the CCD analysis, we investigated the induction start-up time, the isopropylthio-D-galactoside （IPTG） concentration, the post-induction time, and the temperature by response surface methodology. We found that the highest level of ALF fusion protein was achieved in the medium containing 1.89 g/L （NH4）2SO4 and 3.18 g/L KH2PO4, with a cell optical density of 0.8 at 600 nm before induction, an IPTG concentration of 0.5 mmol/L, a post-induction temperature of 32.7~C, and a post-induction time of 4 h. Applying the whole optimization strategy using all optimal factors improved the target protein content from 6.1% （without optimization） to 13.2%. We further applied the optimized medium and conditions in high cell density cultivation, and determined that the soluble target protein constituted 105% of the total protein. Our identification of the economic medium composition, optimal culture conditions, and details of the fermentation process should facilitate the potential application of ALF for further research.

Quantitative analysis of biotechnical reinforcement for a steep slope consisting of composite coal-gangue-soil medium adjacent to a mined-out area

The engineering and geological characteristics of a steep slope consisting of coal gangue, rock and soil medium in Huating coal mine have been comprehensively investigated. Owing to humid weather, heavy rainfall, vegetation and porous characteristics of the soil and rock mass, the steep slope will be destabilized and induce mud-rock flow or derive hazard easily. Firstly, based on the classical slope reinforcement theory, some regularity between the shear and displacement in the destabilized zone of the slope with or without root strength contribution is presented. Then, based on the experimental and statistical analysis of root strength, hydrological characteristics and stability status, etc., some possible biotechnical techniques for reinforcement of the steep slope have been suggested. These methods are important for quantitative analysis of destabilization of the slope and design of the biotechnical reinforcement.

Theoretical solution of transient heat conduction problem in one-dimensional double-layer composite medium

To make heat conduction equation embody the essence of physical phenomenon under study, dimensionless factors were introduced and the transient heat conduction equation and its boundary conditions were transformed to dimensionless forms. Then, a theoretical solution model of transient heat conduction problem in one-dimensional double-layer composite medium was built utilizing the natural eigenfunction expansion method. In order to verify the validity of the model, the results of the above theoretical solution were compared with those of finite element method. The results by the two methods are in a good agreement. The maximum errors by the two methods appear when τ（τ is nondimensional time） equals 0.1 near the boundaries of ζ =1 （ζ is nondimensional space coordinate） and ζ =4. As τ increases, the error decreases gradually, and when τ =5 the results of both solutions have almost no change with the variation of coordinate 4.

Microstructure and Corrosion Resistance of a Novel AlNiLa Lightweight Medium Entropy Amorphous Alloy Composites

A new type of lightweight AlNiLa medium entropy amorphous alloy composite ribbons(labled as MEAAC ribbons)were prepared by vacuum arc melting technology and high-speed single roller meltspinning method.The microstructure and thermal stability of MEAAC ribbons were examined using X-ray diffraction,differential scanning calorimeter,and scanning electron microscope.Meanwhile,the hardness and surface roughness of these ribbons were measured by Vickers microhardness tester and atomic force microscope.The potentiodynamic polarization curves and electrochemical impedance spectroscopy(EIS)were applied to investigate the corrosion behavior of these MEAAC ribbons in simulated seawater(3.5wt%NaCl corrosive solution)at room temperature.The results demonstrate that AlNiLa MEAAC ribbons in the as-received state are mainly composed of amorphous phase and intermetallic compounds.The hardness values of all melt-spun ribbons are above 310 HV_(0.1).With the increase of Al content,the linear polarization resistances of four various AlNiLa MEAAC ribbons are negligibly different numerically.It is also found that Al_(45)Ni_(27.5)La_(27.5) MEAAC ribbons have the most positive corrosion potential and the smallest corrosion current density at the same time;hence it may be a kind of potential material for metal surface protection in harsh ocean environment.

组分梯度膜的有效介电常数的推导

从静电场的边值关系出发,利用平均场理论和有效媒质理论近似理论,研究组分梯度膜的有效介电常数,推导出组分梯度膜的有效介电常数。

Investigating and Mitigating Failure Modes in Physics-Informed Neural Networks(PINNs)

This paper explores the difficulties in solving partial differential equations(PDEs)using physics-informed neural networks(PINNs).PINNs use physics as a regularization term in the objective function.However,a drawback of this approach is the requirement for manual hyperparameter tuning,making it impractical in the absence of validation data or prior knowledge of the solution.Our investigations of the loss landscapes and backpropagated gradients in the presence of physics reveal that existing methods produce non-convex loss landscapes that are hard to navigate.Our findings demonstrate that high-order PDEs contaminate backpropagated gradients and hinder convergence.To address these challenges,we introduce a novel method that bypasses the calculation of high-order derivative operators and mitigates the contamination of backpropagated gradients.Consequently,we reduce the dimension of the search space and make learning PDEs with non-smooth solutions feasible.Our method also provides a mechanism to focus on complex regions of the domain.Besides,we present a dual unconstrained formulation based on Lagrange multiplier method to enforce equality constraints on the model’s prediction,with adaptive and independent learning rates inspired by adaptive subgradient methods.We apply our approach to solve various linear and non-linear PDEs.

MULTISCALE FINITE ELEMENT METHOD FOR SUBDIVIDED PERIODIC ELASTIC STRUCTURES OF COMPOSITE MATERIALS

Deals with a study which discussed the mechanical behaviour for subdivided periodic elastic structures of composite materials, from the viewpoint of macro- and meso-scale coupling. Multiscale asymptotic expansion and truncation error estimates; Discussion on multiscale finite element method; Details of higher order difference quotients and total error estimates; Numerical experiments.

Electrical properties of 0-30.5(Ba_(0.7)Ca_(0.3))TiO_(3)-0.5Ba(Zr_(0.2)Ti_(0.8))O_(3)/PVDF nanocomposites

Lead-free x(0.50(Ba_(0.7)Ca_(0.3)TiO_(3)-0.50Ba(Zr_(0.2)Ti_(0.8)O_(3)-(1-x)PVDF ceramic–polymer nanocomposites with x=0.025,0.05,0.10,0.15,0.20,0.25 were prepared using melt-mixing technique.The distribution of nanoceramic filler particles(0.50(Ba_(0.7)Ca_(0.3)TiO_(3)-0.50Ba(Zr_(0.2)Ti_(0.8)O_(3)in the PVDF matrix were examined using scanning electron microscope.Impedance analysis indicated the negative temperature coefficient of resistance character of all the test specimens.Filler concentrationdependent piezoelectric coefficient(d33Þdata followed exponential growth types of variation.The data for ac conductivity were found to obey Jonscher’s power law.The correlated barrier hopping(CBH)model was found to explain the mechanism of charge transport occurring in the system.The low value of loss tangent(-10^(-2))along with the high value of d_(33) foreshadowing the prospect of present nanocomposite is a better nonlead option for piezo-sensing/detection applications,especially in bio-medical area.

Electrical and magnetic properties of 0-3 Ba(Fe_(1/2)Nb_(1/2))O_(3)/PVDF composites

Lead-free Ba(Fe_(1/2)Nb_(1/2))O_(3)/PVDF O_(3) composites were fabricated using melt.mixing technique.X-ray diffraction,scanning electron microscopy,dielectric,impedance,ac conductivity,magnetic force microscopy(MFM)and vibrating sample magne-tometer studies were undertaken to characterize the samples.Average crstallite size of the Ba(Fe_(1/2)Nb_(1/2))O_(3) powder,estimated using Williamson-Hall approach,was found to be~42 nm.The fller particles of~0.5-1 pum were found to disperse in the polymer matrix of all the composites.Filler concentration dependent values of real and imaginary parts of complex permittivity showed increasing trend and were seen to follow Bruggeman and Furukawa equations.The data for ac conductivity exhibited negative temperature coeficient of resistance character of the test materials and were found to obey Jonscher's power law.The correlated barier hopping model was found to explain satisfactorily the mechanism of charge transport occurring in the system.MFM confirmed the presence of magnetic phases in the composites.Typical magnetization versus applied field curves indicated the possibility of magnetoelectric coupling in the system.Hence,the present composites have shown themselves as potential multi-functional candidate materials for use in high density data storage applications.