Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis

Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery after spinal cord injury remains unclear.In the present study,we established a rat model of spinal cord injury based on impact injury from a dropped weight and then intraperitoneally injected the rats with conditioned medium from human dental pulp stem cells.We found that the conditioned medium effectively promoted the recovery of sensory and motor functions in rats with spinal cord injury,decreased expression of the microglial pyroptosis markers NLRP3,GSDMD,caspase-1,and interleukin-1β,promoted axonal and myelin regeneration,and inhibited the formation of glial scars.In addition,in a lipopolysaccharide-induced BV2 microglia model,conditioned medium from human dental pulp stem cells protected cells from pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway.These results indicate that conditioned medium from human dental pulp stem cells can reduce microglial pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway,thereby promoting the recovery of neurological function after spinal cord injury.Therefore,conditioned medium from human dental pulp stem cells may become an alternative therapy for spinal cord injury.

Generation of breathing solitons in the propagation and interactions of Airy–Gaussian beams in a cubic–quintic nonlinear medium

Using the split-step Fourier transform method, we numerically investigate the generation of breathing solitons in the propagation and interactions of Airy–Gaussian（AiG） beams in a cubic–quintic nonlinear medium in one transverse dimension. We show that the propagation of single AiG beams can generate stable breathing solitons that do not accelerate within a certain initial power range. The propagation direction of these breathing solitons can be controlled by introducing a launch angle to the incident AiG beams. When two AiG beams accelerated in opposite directions interact with each other,different breathing solitons and soliton pairs are observed by adjusting the phase shift, the beam interval, the amplitudes,and the light field distribution of the initial AiG beams.

HOMOGENIZATION OF A STATIONARY NAVIER-STOKES FLOW IN POROUS MEDIUM WITH THIN FILM

The article studies the homogenization of a stationary Navier-Stokes fluid in porous medium with thin film under Dirichlet boundary condition.At the end of the article,＂Darcy＇s law＂is rigorously derived from this model as the parameter ε tends to zero,which is independent of the coordinates towards the thickness.

Adenosine A_(2A)receptor blockade attenuates excitotoxicity in rat striatal medium spiny neurons during an ischemic-like insult

During brain ischemia,excitotoxicity and peri-infarct depolarization injuries occur and cause cerebral tissue damage.Indeed,anoxic depolarization,consisting of massive neuronal depolarization due to the loss of membrane ion gradients,occurs in vivo or in vitro during an energy failure.The neuromodulator adenosine is released in huge amounts during cerebral ischemia and exerts its effects by activating specific metabotropic receptors,namely:A_(1),A_(2A),A_(2B),and A_(3).The A_(2A)receptor subtype is highly expressed in striatal medium spiny neurons,which are particularly susceptible to ischemic damage.Evidence indicates that the A2Areceptors are upregulated in the rat striatum after stroke and the selective antagonist SCH58261 protects from exaggerated glutamate release within the first 4 hours from the insult and alleviates neurological impairment and histological injury in the following 24 hours.We recently added new knowledge to the mechanisms by which the adenosine A2Areceptor subtype participates in ischemia-induced neuronal death by performing patch-clamp recordings from medium spiny neurons in rat striatal brain slices exposed to oxygen and glucose deprivation.We demonstrated that the selective block of A2Areceptors by SCH58261 significantly reduced ionic imbalance and delayed the anoxic depolarization in medium spiny neurons during oxygen and glucose deprivation and that the mechanism involves voltage-gated K+channel modulation and a presynaptic inhibition of glutamate release by the A2Areceptor antagonist.The present review summarizes the latest findings in the literature about the possibility of developing selective ligands of A2Areceptors as advantageous therapeutic tools that may contribute to counteracting neurodegeneration after brain ischemia.

Enhancing Erectile Function and Alleviating Andropause Symptoms: Clinical Efficacy of a Human Stem Cell Conditioned Medium Cream

Erectile dysfunction (ED) is increasingly prevalent in Japan, exceeding 30%, and increasing with age. Unhealthy lifestyle habits, obesity, insufficient exercise, and smoking have been implicated in its pathogenesis, along with endothelial dysfunction of the corpora cavernosa and impaired blood flow to the penis considered underlying factors. However, the current treatments are limited to Phosphodiesterase-5 (PDE5) inhibitors. ED is the primary symptom of andropathy. This study reports the clinical efficacy of human stem cell-conditioned medium cream for ED treatment. Ten men without underlying diseases suspected of andropause with ED (mean age 43.2 ± 4.4 y, Hb 15.2 ± 0.6 gm/dL, AST/ALT 30.2/37.9 ± 12.4/14.0, eGFR 82.7 ± 12.4 mL/min/1.73 m2) were targeted. The cream was applied twice daily to the genital and scrotal areas. The erectile hardness score (EHS), International Index of Erectile Function-5 (IIEF-5), and Aging Male Symptoms (AMS) scale were used to evaluate the participants before and 30 days after use, and the results were compared using paired t-tests. The post-use qualitative opinions were collected through interviews. Significant improvements were observed compared to baseline in the IIEF-5 (11.8 ± 4.6→17.2 ± 5.1, P < 0.001), and AMS (46.3 ± 6.7→37.6 ± 5.3, P < 0.001) scores post cream use. EHS did not show a statistically significant difference, but a trend towards improvement was observed. Qualitative feedback included increased morning erection, improved maintenance of erection during intercourse, and reduced post work fatigue. Human stem cell-conditioned medium contains endothelial growth factors that potentially contribute to the improvement of ED and andropause by enhancing corporal endothelial function. Future studies should include control groups to further investigate the efficacy of these treatments.

Casson Nanofluid Flow with Cattaneo-Christov Heat Flux and Chemical Reaction Past a Stretching Sheet in the Presence of Porous Medium

In the current work,inclined magnetic field,thermal radiation,and the Cattaneo-Christov heat flux are taken into account as we analyze the impact of chemical reaction on magneto-hydrodynamic Casson nanofluid flow on a stretching sheet.Modified Buongiorno’s nanofluid model has been used to model the flow governing equations.The stretching surface is embedded in a porousmedium.By using similarity transformations,the nonlinear partial differential equations are transformed into a set of dimensionless ordinary differential equations.The numerical solution of transformed dimensionless equations is achieved by applying the shooting procedure together with Rung-Kutta 4th-order method employing MATLAB.The impact of significant parameters on the velocity profile f(ζ),temperature distributionθ(ζ),concentration profileϕ(ζ),skin friction coefficient(Cf),Nusselt number(Nux)and Sherwood number(Shx)are analyzed and displayed in graphical and tabular formats.With an increase in Casson fluid 0.5<β<2,the motion of the Casson fluid decelerates whereas the temperature profile increases.As the thermal relation factor expands 0.1<γ1<0.4,the temperature reduces,and consequently thermal boundary layer shrinks.Additionally,by raising the level of thermal radiation 1<Rd<7,the temperature profile significantly improves,and an abrupt expansion has also been observed in the associated thermal boundary with raise thermal radiation strength.It was observed that higher permeability 0<K<4 hinders the acceleration of Casson fluid.Higher Brownian motion levels 0.2<Nb<0.6 correspond to lower levels of the Casson fluid concentration profile.Moreover,it is observed that chemical reaction 0.2<γ2<0.5 has an inverse relation with the concentration level of Casson fluid.The current model’s significant uses include heat energy enhancement,petroleum recovery,energy devices,food manufacturing processes,and cooling device adjustment,among others.Furthermore,present outcomes have been found in great agreementwith already publishedwork.

Assessing Factors Influencing Solar Energy Adoption among Small and Medium Enterprises in Mzuzu City, Malawi

One of the many renewable energy sources that offer advantages is solar energy, which also lowers energy prices and promotes environmental sustainability and energy security. Despite these advantages, various barriers, such as installation costs, have prevented small and medium-sized enterprises from investigating this invention. Malawi has a significant energy shortfall such that most businesses have been hindered from their profit maximization goals. The “Photovoltaic systems” (PV) that transform sunlight into electricity are the subject of this study. This type of solar energy system is situated on the building’s roof and generally produces electricity for businesses and even homes. Solar energy offers a great impact to small and medium enterprises in Mzuzu city with a cost-effective and dependable alternative to energy that has the potential to change the game. Therefore the aim of the study was to identify factors that encourage the adoption of solar energy among small medium enterprises in the city of Mzuzu city. And to identify some of barriers faced when adopting solar energy among small and medium enterprises in the city of Mzuzu. The research approach employed in the study was a survey. A survey is a type of research methodology in which primary data is gathered from a sample using a questionnaire. When information is to be gathered from a wider sample, a survey is employed. A bigger sample size was needed in this study in order to facilitate hypothesis testing. It is advised to apply a logical approach while using the survey. The survey utilized a five-point Likert scale. The study used convenience sampling to select study participants. The sample size in this study was determined using Cochran’s sample size formula. Statistical Package for Social Sciences (SPSS) and Microsoft Excel were used for statistical analysis. About 97.2% of the participants were aware of solar as a source of energy compared to 2.8 % who were unaware. The majority of participants use solar energy for lighting only, seconded by those who use electricity. The least number of participants use solar energy for cooling only. The majority of participants 21.5% indicated partnership and collaboration as the most motivating factor for the adoption of solar energy. This was followed by technical expertise 19.1 % the least number of participants 10.8% expressed that policy and regulatory frameworks were associated with the adoption of solar energy. This study found that there are no statistically significant factors influencing barriers to the adoption of solar energy. The price of solar energy adoption was identified as the least factor associated with the acceptance or rejection of solar energy. Nonetheless, the reasons given by the homes that had embraced solar technology aligned with the findings of other studies. This survey also found that although the public was aware of solar energy, and technology, there were still a number of factors that mattered, especially for non-adopters.

Preparation of FeCoNi medium entropy alloy from Fe^(3+)-Co^(2+)-Ni^(2+)solution system

In recent years,medium entropy alloys have become a research hotspot due to their excellent physical and chemical performances.By controlling reasonable elemental composition and processing parameters,the medium entropy alloys can exhibit similar properties to high entropy alloys and have lower costs.In this paper,a FeCoNi medium entropy alloy precursor was prepared via sol-gel and coprecipitation methods,respectively,and FeCoNi medium entropy alloys were prepared by carbothermal and hydrogen reduction.The phases and magnetic properties of FeCoNi medium entropy alloy were investigated.Results showed that FeCoNi medium entropy alloy was produced by carbothermal and hydrogen reduction at 1500℃.Some carbon was detected in the FeCoNi medium entropy alloy prepared by carbothermal reduction.The alloy prepared by hydrogen reduction was uniform and showed a relatively high purity.Moreover,the hydrogen reduction product exhibited better saturation magnetization and lower coercivity.

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve repair.Cell-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular communication.Nevertheless,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain unclear.To investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose deprivation.The mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest cells.We performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest cells.Subsequently,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded miR-21-5p.Finally,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously restored.These findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in miRNA-21-5p.miRNA-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell secretome.This helps to explain the mechanism of action of the secretome and extracellular vesicles of neural crest cells in repairing damaged peripheral nerves,and also promotes the application of miR-21-5p in tissue engineering regeneration medicine.

Effect of Magnetohydrodynamic on Thin Films of Unsteady Micropolar Fluid through a Porous Medium

In This paper, we deal with the study of the effect of magnetohydrodynamic on thin films of unsteady micropolar fluid through a porous medium. These Thin films are considered for three different geometries. The governing continuity, momentum and angular momentum equations are converted into a system of non-linear ordinary differential equations by means of similarity transformation. The resulting system of coupled non-linear ordinary differential equations is solved numerically by using shooting method. A representative set of numerical results in the three thin film flow problems for velocity and micro-rotation profiles are discussed and presented graphically. A comprehensive parametric study is carried out to show the effects of the micropolar fluid parameters, magnetic field parameter, permeability parameter and etc. on the obtained solutions.

The Thinking of Financing under Dual Economies Character In Medium & Small Enterprises

In China,the dual economies show prominence in financial field.It leads to the serious dual credit ration and becomes one of the main cause which makes financing in medium & small sized enterprises becomes harder and harder.On basis of this situation,we theoretically pose that we can alleviate the difficulty through the dual cooperation of financial control and the deepening of finance.

Raising medium function of environmental engineering by using life rubbish to produce carpet turf

Abundant material with low cost was utilized to raise medium function of producing carpet turf by life rubbish. The results showed that the material used could strikingly raise water retentiveness and ventilating of medium. Through the determination of the effect of each growth index of several turfgrass, emergence density, plant height, root growth and individual plant′s net primary production were all positively related to the material′s amount mixed, showing that the mixture of material could promote turfgrass growth, further proving that the material used raised medium function of producing carpet turf by life rubbish and made most use of life rubbish resources. It provided a scientific base is for application of environmental engineering by using life rubbish to produce carpet turf. So the study had both important theoretic meaning and applied value.

Analytical solutions for dynamic pressures of coupling fluid-solid-porous medium due to P wave incidence

Wave reflection and refraction in layered media is a topic closely related to seismology,acoustics,geophysics and earthquake engineering.Analytical solutions for wave reflection and refraction coefficients in multi-layered media subjected to P wave incidence from the elastic half-space are derived in terms of displacement potentials.The system is composed of ideal fluid,porous medium,and underlying elastic solid.By numerical examples,the effects of porous medium and the incident wave angle on the dynamic pressures of ideal fluid are analyzed.The results show that the existence of the porous medium,especially in the partially saturated case,may significantly affect the dynamic pressures of the overlying fluid.

Application of response surface methodology in medium optimization for pyruvic acid production of Torulopsis glabrata TP19 in batch fermentation

Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows: ammonium sulfate 0.7498 (10.75 g/L), glucose 0.9383 (109.38 g/L) and nicotinic acid 0.3633 (7.86 mg/L) with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient (R2) was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.

Isolation of Leptospirillum ferriphilum by single-layered solid medium

According to physiological and biochemical characteristics of Leptospirillum ferriphilum, a strain of object bacteria was isolated successfully. Bacteria were enriched by selective liquid medium and plated on designed single-layered agar solid medium. Colony was cultured and bacteria were collected. The morphologies of the object bacteria were observed using crystal violet staining, scanning electron microscope（SEM） and transmission electron microscope （TEM）. The result of 16S rDNA identification shows that this bacterium belongs to Leptospirillum ferriphilum and it is named as Leptospirillum ferriphilum strain D1. These results indicate that this new single-layered agar solid medium is efficient and physiological-biochemical characteristics show that the optimum simple for isolation of Leptospirillum ferriphilum. Additionally, initial pH value and its growth temperature are 1.68 and 40℃.

Coupled thermo-hydro-mechanical-migratory model for dual-porosity medium and numerical analysis

A coupled thermo-hydro-mechanical-migratory model of dual-porosity medium for saturated-unsaturated ubiquitous-joint rockmass was established,in which the stress field and the temperature field were single,but the seepage field and the concentration field were double,and the influences of sets,spaces,angles,continuity ratios,stiffnesses of fractures on the constitutive relationship of the medium were considered.Also,the relative two-dimensional program of finite element method was developed.Taking a hypothetical nuclear waste repository as a calculation example,the case in which the rockmass was unsaturated dual-porosity medium and radioactive nuclide leak was simulated numerically,and the temperatures,negative pore pressures,saturations,flow velocities,nuclide concentrations and principal stresses in the rockmass were investigated.The results show that the negative pore pressures and nuclide concentrations in the porosity and fracture present different changes and distributions.Even though the saturation degree in porosity is only about 1/10 that in fracture,the flow velocity of underground water in fracture is about three times that in porosity because the permeability coefficient of fracture is almost four orders higher than that of porosity.The value of nuclide concentration in fracture is close to that in porosity.

Influence of light intensity and water content of medium on total dendrobine of Dendrobium nobile Lindl

Objective: To ascertain the influence of light intensity and water content of medium on the total dendrobine of Dendrobium nobile(D. nobile).Method: The principal component analysis combined with total dendrobine accumulation was conducted to assess the yield and quality of D. nobile in all treatments. In the experiment, D. nobile plants were cultivated in greenhouse as tested materials, and complete test of 9 treatments was adopted with relative light intensities 75.02%, 39.74%,29.93% and relative water content of medium 50%, 65%, 80%. The plants were treated in June and harvested till December. Indexes including agronomic traits, fresh weight and dry weight of stem and leaf, ash content, extract, and dendrobine were measured.Results: Under the light intensity treatments of 75.02% with 50%, 65%, 80% water content of medium, the basal stems of plants were comparatively thicker with more leaves, and the fresh weight and dry weight of stems and leaves were significantly higher than other 6 treatments.Leaves in all treatments contained dendrobine. Under the light intensity treatments of 75.02%with 50%, 65%, 80% water content of medium, dendrobine content of leaves was lower while dendrobine contents of other treatments were more than 0.60%. After comprehensive assessment through the principal component analysis and total dendrobine accumulation, the results showed that 3 treatments with relative light intensity of 75.02% ranked the top three.Conclusions: In brief, the moderately strong light intensity and water content of medium from low to medium can facilitate the growth and yield of D. nobile plants, while light intensity from moderately weak to weak can enhance the dendrobine content.

Endothelial progenitor cell-conditioned medium promotes angiogenesis and is neuroprotective after spinal cord injury

Endothelial progenitor cells secrete a variety of growth factors that inhibit inflammation, promote angiogenesis and exert neuroprotective effects. Therefore, in this study, we investigated whether endothelial progenitor cell-conditioned medium might have therapeutic effectiveness for the treatment of spinal cord injury using both in vitro and in vivo experiments. After primary culture of bone marrow-derived macrophages, lipopolysaccharide stimulation was used to classically activate macrophages to their proinflammatory phenotype. These cells were then treated with endothelial progenitor cell-conditioned medium or control medium. Polymerase chain reaction was used to determine mR NA expression levels of related inflammatory factors. Afterwards, primary cultures of rat spinal cord neuronal cells were prepared and treated with H2O2and either endothelial progenitor cell-conditioned medium or control medium. Hoechst 33258 and propidium iodide staining were used to calculate the proportion of neurons undergoing apoptosis. Aortic ring assay was performed to assess the effect of endothelial progenitor cell-conditioned medium on angiogenesis. Compared with control medium, endothelial progenitor cell-conditioned medium mitigated the macrophage inflammatory response at the spinal cord injury site, suppressed apoptosis, and promoted angiogenesis. Next, we used a rat model of spinal cord injury to examine the effects of the endothelial progenitor cell-conditioned medium in vivo. The rats were randomly administered intraperitoneal injection of PBS, control medium or endothelial progenitor cell-conditioned medium, once a day, for 6 consecutive weeks. Immunohistochemistry was used to observe neuronal morphology. Terminal deoxynucleotidyl transferase-mediated d UTP nick-end labeling assay was performed to detect the proportion of apoptotic neurons in the gray matter. The Basso, Beattie and Bresnahan Locomotor Rating Scale was used to evaluate the recovery of motor function of the bilateral hind limbs after spinal cord injury. Compared with the other two groups, the number of axons was increased, cavities in the spinal cord were decreased, the proportion of apoptotic neurons in the gray matter was reduced, and the Basso, Beattie and Bresnahan score was higher in the endothelial progenitor cell-conditioned medium group. Taken together, the in vivo and in vitro results suggest that endothelial progenitor cell-conditioned medium suppresses inflammation, promotes angiogenesis, provides neuroprotection, and promotes functional recovery after spinal cord injury.

Application of Multi-agent Models to Urban Expansion in Medium and Small Cities: A Case Study in Fuyang City,Zhejiang Province,China

In this study, three-phase satellite images were used to define rules for the allocation of time and space in construction land resources based on a complex adaptive system and game theory. The decision behavior and rules of government agent, enterprise agent and resident agent in construction land growth were explored. A distinctive and dynamic simulation model of construction land growth was built, which integrated multi-agent, GIS technology and RS data and described the interaction among influencing agents, Taking Fuyang City in the Changjiang River Delta as an example, an assessment process for the remote sensing data in construction land and scenario planning was constructed. Repast and ArcGIS were used as simulation platforms. A simulation of the spatial pattern in land-use planning and the setting of scenario planning were conducted by using the incomplete active game, which was based on different natural, social and economic levels. Through this model, a simulation of urban planning space and decision-making for Fuyang City was created. Relevant non-structured problems arising from urban planning management could be identified, and the process and logic of urban planning spatial decision-making could thus be improved. Cell-by-cell comparison showed that the simulation accuracy was over 72%. This model has great potential for use by government and town planners in decision support and technique support in the policy-making process.

Effect of Heat Sink and Cooling Mediums on Ferrite Austenite Ratio and Distortion in Laser Welding of Duplex Stainless Steel 2205

In order to control the ferrite and austenite percentage in duplex stainless steel welding, many researchers try to change the laser welding parameters and cooling medium, but ignore to study the influence of heat sink effect on weld strength. In this work, the effect of aluminium heat sink and varying cooling medium on the laser welding of duplex stainless steel (DSS) 2205 is studied. The 2 mm thick DSS sheets welded with pulsed Nd: YAG laser welding machine by varying the cooling medium (air and oil) and an aluminium plate used as a heat sink. The welded specimens tested for tensile strength, micro-hardness, distortion, microstructure and radiography analysis. The faster cooling rate in the oil quenching process enhances the ferrite percentage compared with air-cooled samples. But the faster cooling rate in oil quenching leads to more distortion and using aluminium as a heat sink influenced positively the distortion to a small extent. The lower cooling rate in air quenching leads to a higher tensile strength of the welded specimen. The objective of this work is to analyse experimentally the effect of cooling medium and heat sink in the mechanical and metallurgical properties of laser welded duplex stainless steel.