MOTHER OF FT AND TFL1(MFT)属于磷脂酰乙醇胺结合蛋白(phosphatidyl ethanolamine-binding protein,PEBP)家族,该家族在被子植物中主要起着促进或抑制开花和控制株型的作用。本课题组前期从海岛棉(Gossypium barbadense)中克隆了一个MF...MOTHER OF FT AND TFL1(MFT)属于磷脂酰乙醇胺结合蛋白(phosphatidyl ethanolamine-binding protein,PEBP)家族,该家族在被子植物中主要起着促进或抑制开花和控制株型的作用。本课题组前期从海岛棉(Gossypium barbadense)中克隆了一个MFT-like同源基因GbMFT2,发现其在种子中表达量较高,并且受ABA诱导。为了进一步探究该基因的功能,本研究首先检测了200 mmol/L Na Cl胁迫海岛棉0、1 h、3 h、6 h、12 h后基因的表达量,表明在盐诱导1 h GbMFT2基因的表达量最高。然后构建了过量表达载体p35S:GbMFT2,并通过农杆菌介导的叶盘法将其转化到普通烟草(Nicotiana tabacum cv.NC89)中,半定量RT-PCR表明GbMFT2基因在烟草中异位表达成功。利用0、100 mmol/L、200 mmol/L、300 mmol/L、400 mmol/L和500 mmol/L Na Cl胁迫不同的转基因株系,表明转基因烟草比野生型烟草更耐盐。再分别测量300 mmol/L Na Cl处理前后的野生型烟草和转基因烟草的脯氨酸含量、POD活性,发现处理后的野生型烟草的脯氨酸含量、POD活性均明显降低,而转基因烟草株系中的脯氨酸含量、POD活性均有明显的提升。研究结果表明,GbMFT2基因能够响应盐胁迫,并增加烟草的耐盐性。本研究可为研究植物MFT-like基因的功能提供了新思路,也为进一步解析棉花发育基因的功能提供了研究基础。展开更多
The power electronic transformer(PET) has recently emerged as a type of power converter. It features the basic functions of power conversion and isolation as well as additional functions related to power quality contr...The power electronic transformer(PET) has recently emerged as a type of power converter. It features the basic functions of power conversion and isolation as well as additional functions related to power quality control. A novel PET for a distribution grid called a flexible power distribution unit is proposed in this paper, and the energy exchange mechanism between the network and the load is revealed. A 30 kW 600 VAC/220 VAC/110 VDC medium-frequency isolated prototype is developed and demonstrated. This paper also presents key control strategies of the PET for electrical distribution grid applications,especially under grid voltage disturbance conditions.Moreover, stability issues related to the grid-connected three-phase PET are discussed and verified with an impedance-based analysis. The PET prototype is tested, and it passes the voltage-disturbance ride-through function. The experimental results verify the power quality control abilities of the PET.展开更多
文摘MOTHER OF FT AND TFL1(MFT)属于磷脂酰乙醇胺结合蛋白(phosphatidyl ethanolamine-binding protein,PEBP)家族,该家族在被子植物中主要起着促进或抑制开花和控制株型的作用。本课题组前期从海岛棉(Gossypium barbadense)中克隆了一个MFT-like同源基因GbMFT2,发现其在种子中表达量较高,并且受ABA诱导。为了进一步探究该基因的功能,本研究首先检测了200 mmol/L Na Cl胁迫海岛棉0、1 h、3 h、6 h、12 h后基因的表达量,表明在盐诱导1 h GbMFT2基因的表达量最高。然后构建了过量表达载体p35S:GbMFT2,并通过农杆菌介导的叶盘法将其转化到普通烟草(Nicotiana tabacum cv.NC89)中,半定量RT-PCR表明GbMFT2基因在烟草中异位表达成功。利用0、100 mmol/L、200 mmol/L、300 mmol/L、400 mmol/L和500 mmol/L Na Cl胁迫不同的转基因株系,表明转基因烟草比野生型烟草更耐盐。再分别测量300 mmol/L Na Cl处理前后的野生型烟草和转基因烟草的脯氨酸含量、POD活性,发现处理后的野生型烟草的脯氨酸含量、POD活性均明显降低,而转基因烟草株系中的脯氨酸含量、POD活性均有明显的提升。研究结果表明,GbMFT2基因能够响应盐胁迫,并增加烟草的耐盐性。本研究可为研究植物MFT-like基因的功能提供了新思路,也为进一步解析棉花发育基因的功能提供了研究基础。
基金supported in part by the National Basic Research Program of China(No.2016YFB0900404)the National Natural Science Foundation of China(No.51477030,No.51207023)+2 种基金the Cooperative Innovation Fund of Jiangsu Province–the Prospective and Joint Research Project(No.BY2015070-18)the Basic and Prospective Science and Technology Project of State Grid Corporation of China(No.PD71-17-024)the Fundamental Research Funds for the Central Universities(No.2242017K40159)
文摘The power electronic transformer(PET) has recently emerged as a type of power converter. It features the basic functions of power conversion and isolation as well as additional functions related to power quality control. A novel PET for a distribution grid called a flexible power distribution unit is proposed in this paper, and the energy exchange mechanism between the network and the load is revealed. A 30 kW 600 VAC/220 VAC/110 VDC medium-frequency isolated prototype is developed and demonstrated. This paper also presents key control strategies of the PET for electrical distribution grid applications,especially under grid voltage disturbance conditions.Moreover, stability issues related to the grid-connected three-phase PET are discussed and verified with an impedance-based analysis. The PET prototype is tested, and it passes the voltage-disturbance ride-through function. The experimental results verify the power quality control abilities of the PET.
