Aim： To determine the deletion junctions of infertile men in Taiwan with azoospermia factor region c （AZFc） deletions and to evaluate the genotype/phenotype correlation. Methods： Genomic DNAs from 460 infertile me...Aim： To determine the deletion junctions of infertile men in Taiwan with azoospermia factor region c （AZFc） deletions and to evaluate the genotype/phenotype correlation. Methods： Genomic DNAs from 460 infertile men were examined. Bacterial artificial chromosome clones were used to verify the accuracy of polymerase chain reaction. Deletion junctions of the AZFc region were determined by analysis of sequence-tagged sites and gene-specific markers. Results： Complete AZFc deletions, including BPY2, CDY1 and DAZ genes, were identified in 24 men. The proximal breakpoints were clustered between sY1197 and sY1192, and the distal breakpoints were clustered between sY1054 and sYl125 in all but one of the 24 men. The testicular phenotypes of men with complete AZFc deletion varied from oligozoospermia, to hypospematogenesis, to maturation arrest. Conclusion： We identified a group of infertile men with uniform deletion junctions of AZFc in the Taiwan population. Despite this homogeneous genetic defect in the AZFc region, no clear genotypedphenotype correlation could be demonstrated. （Asian JAndrol 2006 Mar; 8： 205-211）展开更多
AIM: To investigate expression of factors related to angiogenesis: HIF-1 alpha, iNOS, COX-2 and VEGF in choroidal melanoma and its clinical significance. METHODS: Fifty samples of choroidal melanoma and 15 samples of ...AIM: To investigate expression of factors related to angiogenesis: HIF-1 alpha, iNOS, COX-2 and VEGF in choroidal melanoma and its clinical significance. METHODS: Fifty samples of choroidal melanoma and 15 samples of melanocytic nevi of the eyelid identified by pathology were collected. Immunohistochemistry SP method was used to examine the expression of HIF-1 alpha, iNOS, COX-2 and VEGF in these samples. The comparison among groups was done by SPSS 13.0 software. RESULTS: The positive expression rates of HIF-1 alpha, iNOS, COX-2 and VEGF in choroidal melanoma group were significantly higher than those in eyelid nevi group (chi(2)= 6.5542, 7.7224, 8.5828, 15.1749). The positive expression rate of VEGF was associated with the tumor size (chi(2)= 10.9194), but was not associated with pathological type (chi(2)=2.0712) and the situation of scleral invasion (chi(2)= 5.4289). The positive expression rate of HIF-1 alpha was associated with the tumor size (chi(2)=7.1216) and pathological type (chi(2)=9.0889), but was not associated with the situation of scleral invasion (chi(2)=3.3586). The positive expression rate of iNOS was associated with the tumor size (chi(2)=9.5503), but was not associated with pathological type (chi(2)=1.9450) and the situation of scleral invasion (chi(2)=2.3810). The positive expression rate of COX-2 was associated with the tumor size (chi(2)=7.2970), but was not associated with pathological type (chi(2)=1.8421) and the situation of sclera! invasion (chi(2)= 0.4018). The expression of HIF-1 alpha, iNOS and COX-2 were significantly associated with the expression of VEGF (r = 0.9429, 1, 0.9857). The expression of COX-2 was significantly associated with the expression of iNOS (r=0.9857). The expression of HIF-1 alpha was significantly associated with the expression of COX-2 ( r=0.9857). The expression of HIF-1 alpha was significantly associated with the expression of iNOS (r= 0. 9429). CONCLUSION: The expression of HIF-1 alpha, iNOS and COX-2 protein in choroidal melanoma were higher and may relate to angiogenesis and stimulate tumor growth. Determination of HIF-1 alpha, iNOS and COX-2 may be helpful for the diagnosis and therapy of this tumor.展开更多
文摘Aim： To determine the deletion junctions of infertile men in Taiwan with azoospermia factor region c （AZFc） deletions and to evaluate the genotype/phenotype correlation. Methods： Genomic DNAs from 460 infertile men were examined. Bacterial artificial chromosome clones were used to verify the accuracy of polymerase chain reaction. Deletion junctions of the AZFc region were determined by analysis of sequence-tagged sites and gene-specific markers. Results： Complete AZFc deletions, including BPY2, CDY1 and DAZ genes, were identified in 24 men. The proximal breakpoints were clustered between sY1197 and sY1192, and the distal breakpoints were clustered between sY1054 and sYl125 in all but one of the 24 men. The testicular phenotypes of men with complete AZFc deletion varied from oligozoospermia, to hypospematogenesis, to maturation arrest. Conclusion： We identified a group of infertile men with uniform deletion junctions of AZFc in the Taiwan population. Despite this homogeneous genetic defect in the AZFc region, no clear genotypedphenotype correlation could be demonstrated. （Asian JAndrol 2006 Mar; 8： 205-211）
文摘AIM: To investigate expression of factors related to angiogenesis: HIF-1 alpha, iNOS, COX-2 and VEGF in choroidal melanoma and its clinical significance. METHODS: Fifty samples of choroidal melanoma and 15 samples of melanocytic nevi of the eyelid identified by pathology were collected. Immunohistochemistry SP method was used to examine the expression of HIF-1 alpha, iNOS, COX-2 and VEGF in these samples. The comparison among groups was done by SPSS 13.0 software. RESULTS: The positive expression rates of HIF-1 alpha, iNOS, COX-2 and VEGF in choroidal melanoma group were significantly higher than those in eyelid nevi group (chi(2)= 6.5542, 7.7224, 8.5828, 15.1749). The positive expression rate of VEGF was associated with the tumor size (chi(2)= 10.9194), but was not associated with pathological type (chi(2)=2.0712) and the situation of scleral invasion (chi(2)= 5.4289). The positive expression rate of HIF-1 alpha was associated with the tumor size (chi(2)=7.1216) and pathological type (chi(2)=9.0889), but was not associated with the situation of scleral invasion (chi(2)=3.3586). The positive expression rate of iNOS was associated with the tumor size (chi(2)=9.5503), but was not associated with pathological type (chi(2)=1.9450) and the situation of scleral invasion (chi(2)=2.3810). The positive expression rate of COX-2 was associated with the tumor size (chi(2)=7.2970), but was not associated with pathological type (chi(2)=1.8421) and the situation of sclera! invasion (chi(2)= 0.4018). The expression of HIF-1 alpha, iNOS and COX-2 were significantly associated with the expression of VEGF (r = 0.9429, 1, 0.9857). The expression of COX-2 was significantly associated with the expression of iNOS (r=0.9857). The expression of HIF-1 alpha was significantly associated with the expression of COX-2 ( r=0.9857). The expression of HIF-1 alpha was significantly associated with the expression of iNOS (r= 0. 9429). CONCLUSION: The expression of HIF-1 alpha, iNOS and COX-2 protein in choroidal melanoma were higher and may relate to angiogenesis and stimulate tumor growth. Determination of HIF-1 alpha, iNOS and COX-2 may be helpful for the diagnosis and therapy of this tumor.
