坡地和沼泽地野牡丹(Melastoma candidum)叶片的解剖特征与气孔气体交换特性

对在坡地和沼泽地生长的野牡丹叶的解剖特征与气孔气体交换特性的研究表明:两者在形态结构方面存在着一定的差异,坡地野牡丹叶较窄,上表皮细胞小而排列紧密,气孔密度较大,上表皮厚,沼泽地野牡丹整个叶片和海绵组织比较厚,最大导管直径和栅栏细胞长度/宽度比值大,但栅栏组织厚/海绵组织厚比值较小。坡地野牡丹与沼泽地野牡丹的叶绿素a、叶绿素b的含量和类胡萝卜素的含量以及叶绿素a/b比值的差异都很小。沼泽地野牡丹净光合速率、蒸腾速率及气孔导度较高,常用的水分利用效率(Pn/Tr)接近,沼泽地野牡丹的内在水分利用效率(Pn/Gs)略高于坡地,但差异不显著。综合分析其生境条件,土壤与野牡丹叶片解剖结构和生理生态特性相关性密切。但实验结果不足以确定两种生境下生长的野牡丹为不同的生态型。

Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro

Objective:To screen the cytotoxic activity of Melasloma malabathricum(M,malubathricum)against human breast carreer cell line(MCF-7)in vitro.Methods:A three steps extraction protocol using n-hexane,chloroform and methanol as the solvents systems was carried out on leaves,stems and flowers of M.nalabathricum.Dimethyl sulfoxide was used in extracts dilution and serial dilutions were conducted to obtain five different extract concentrations(100μg/mL,50μg/mL,25μg/mL,123μg/rnL and 6.25μg/mL).The evaluation of cell growth was determined using methylene blue assay.Results:Methanol extract from the leaves showed significant anticancer activity against MCF-7cell lines with the TC_(50)value of 7.14μg/ml while methanol and chloroform extract from the flowers exhibited a moderate activity towards MCF-7 cell line,with the IC_(50)value of 33.63μg/mL and 45.76μg/mL respectively after 72 h of treatment.Conclusions:The extracts from leaves and flowers of M.nulabatkricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC_(50)at 7.14μg/mL while the extracts from stems showed less growth inhibition activity.

Potentials of <i>Melastoma malabathricum</i>Linn. Flower and Fruit Extracts as Antimicrobial Infusions

Melastoma malabathricum Linn. is a shrub that belongs to the family Melastomataceae and a common herbal plant used in folk medicines to treat inflamed wounds. This study was carried out with the aim to evaluate the inhibitory activities of different concentrations of the M. malabathricum Linn. flower and fruit crude extracts against a variety of microorganisms. The inhibitory effects of both extracts were tested against the microorganisms using the disc diffusion method. The lowest concentrations of the extracts producing inhibition zones against the test microorganisms were used to determine their Minimum Inhibitory Concentrations (MICs) and Minimum Microbicidal Concentrations (MMCs). Both crude extracts showed strong inhibitory activities against Gram-positive bacteria. The range of MIC values for the crude flower and fruit extracts on all the bacteria tested were 12.5 to 100.0 mg/ml. Overall, Gram-positive bacteria were more susceptible to the crude extracts compared to Gram-negative species, potentiating a possible use of the extracts to inhibit or kill potential pathogens.

Treatment Effects of Common Melastoma Herb on White Scour in Piglets

[ Objective] To explore the treatment effects of common melastoma herb decoction on white scour in piglets. [ Method] Piglets suffering from white scour were orally given the common melastorna herb decoction and norfloxacin, respectively. [ Result] After the weaned piglets were gavaged medicines, the treatment effect of the common melastoma herb decoction was significantly different from that of norfloxacin （ P 〈 0.05）, and the optimal dosage of common melastoma herb decoction was 20 ml per piglet for treatment. After the weaned piglets were given medicines added in feedstuff, the treatment effect of the common melastoma herb decoction was significantly different from that of norfloxacin （P 〈 0.01 ）, and the optimum dosage of common melastoma herb decoction was 50 ml per piglet for treatment. The preventive effect of the common melastoma herb decoction was significantly different from that of the control group in 35-day＇old piglets suffering from white scour （ P 〈 0.05）, and the optimal dosage of common melastoma herb decoction was 15 ml per piglet for prophylaxis. To mix medicine in feedstuff was the preferred way for the diseased piglets having appetite. [ Cenclusion] The common melastoma herb decoction has treatment effects and preventive effects on white scour of piglets.

Elucidation of the hepatoprotective effect and mechanism of Melastoma dodecandrum Lour. based on network pharmacology and experimental validation

Objective:To systematically explore the effect and mechanism of melastomatis dodecandri herba(Melastoma dodecandrum Lour.)in the treatment of hepatitis based on network pharmacology.Method:We evaluated the hepatoprotective effects of M.dodecandrum in concanavalin A(Con A)-induced hepatitis in mice by assessing survival rate,histological analysis,serum transaminases,and related cytokines.Then the mechanism of action was predicted by a network pharmacology-based strategy.Based on the results,we measured the hepatic expression of related genes at mRNA level and proteins related to the phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt)and nuclear factorkappa B(NF-кB)pathways.Results:Our study results clearly demonstrated that M.dodecandrum pretreatment significantly alleviated liver injury.This was demonstrated by an increase in survival rate,decreased severity of liver damage,and reduced serum transaminase levels compared with those in the Con A group.Moreover,M.dodecandrum significantly reduced the serum levels of tumor necrosis factor-a,interleukin-6,and interferon-g and increased the liver levels of superoxide dismutase,which indicated that M.dodecandrum exhibits anti-inflammatory and antioxidant activities.On the basis of network pharmacology,50 nodes were selected as major hubs based on their topological importance.Pathway enrichment analyses indicated that the putative targets of M.dodecandrum mostly participate in various pathways associated with the anti-inflammation response,which implies the underlying mechanism by which M.dodecandrum acts on hepatitis.Real-time fluorescent quantitative PCR analysis showed that M.dodecandrum downregulates the mRNA expression of interleukin-6,Toll-like receptor 7,interleukin-1 receptor-associated kinase-4,NF-кB and tumor necrosis factor-a in liver tissues.Western blotting showed that M.dodecandrum pretreatment protected against inflammation through activating the PI3K-Akt pathway by upregulating phosphorylated Akt(p-Akt)expression and suppressing NF-кB activation by inhibiting the phosphorylation of IKK,IkBa,and p65.Conclusion:The present work demonstrated the hepatoprotective effects of M.dodecandrum by regulating the PI3K/Akt and NF-кB pathways in Con A-induced mice,which provide insights into the treatment of hepatitis using M.dodecandrum.

嘎狗噜醇提物抗鸭乙型肝炎病毒感染的短期疗效分析

目的探究嘎狗噜醇提物体内抗鸭乙肝病毒(DHBV)及保肝作用。方法建立鸭乙型肝炎病毒感染动物模型,随机分成6组(每组10只):嘎狗噜醇提物高剂量组、中剂量组、低剂量组,安慰剂组,拉米夫定组,非感染组,每日灌胃给药1次,持续28天;在给药前(T_(0))及给药后7 d(T_(7))、14 d(T_(14))、21 d(T_(21))、28 d(T_(28))和停药后5 d(P_(5)),取各组麻鸭静脉血,实时荧光定量PCR法检测鸭血清样本中鸭乙肝病毒(DHBV DNA)水平、酶联免疫吸附法(ELISA)测定鸭乙肝表面抗原(DHBsAg)和鸭乙肝e抗原(DHBeAg),检测ALT和AST水平,停药后6天(P_(6))处死麻鸭并解剖取肝,观察麻鸭肝脏病理切片。结果给药前(T_(0)),基线指标差异无统计学意义(P>0.05)。嘎狗噜醇提物各剂量组均未发现DHBV DNA载量下降,DHBsAg OD值也未见降低(P>0.05),对AST作用不明显;嘎狗噜醇提物各剂量组DHBeAg OD值、ALT均降低(P<0.05);肝脏病理结果显示,嘎狗噜醇提物高剂量组病变程度低于其他剂量组(P<0.05)。结论未发现嘎狗噜醇提物有抑制DHBV、DHBsAg的作用,对AST作用不明显;嘎狗噜醇提物各剂量组均能抑制DHBeAg、降低ALT水平,高剂量组能够改善肝脏病理损伤,对肝脏有一定保护作用。

中药毛稔对伤口愈合的治疗效果与机制预测

目的探究中药毛稔对伤口的愈合的治疗效果并探讨其潜在的作用机制。方法采取药物水煎法获取中药毛稔原液,按50倍、100倍、200倍、400倍稀释后作用于体外培养的人角质形成细胞HaCaT,研究分为对照(CON)组、实验(M50,M100,M200,M400)组,CCK8法检测细胞活力,划痕模型检验伤口愈合效果;液相色谱-质谱联用分析毛稔的代谢产物并构建药物-疾病-靶点相互作用网络预测中药毛稔干预伤口愈合的机制。结果与CON组相比,M50、M100、M200、M400组HaCaT细胞相对增殖率[(112.59±2.04)%、(119.04±2.15)%、(128.40±1.10)%、(133.72±2.83)%]增加(P<0.05)。M400组划痕愈合率在12 h和24 h[(86.93±4.90)%、(95.13±3.70)%]高于CON组[(36.29±2.97)%、(85.01±2.10)%],差异具有统计学意义(P<0.05)。非靶向代谢组学筛选中药毛稔有效活性成分539个,活性成分作用靶点与伤口愈合相交靶点592个。富集分析结果显示,交集靶点主要参与2655个生物过程,富集于92个细胞组分,主要涉及164个分子功能,并涉及180条信号通路。结论中药毛稔能够促进HaCaT细胞增殖和划痕损伤后愈合,可能通过亚麻酸甘油酯、白桦脂醇、L-色氨酸、桑黄素等活性成分作用于TNF、SRC、TP53等靶点达到治疗创伤的作用,其潜在机制可能与PI3K-Akt等信号通路有关,有望成为治疗创伤的有效药物。

畲药地稔水煎液联合艾瑞昔布片对神经根型颈椎病的效果评估

目的评价畲药地稔水煎液(MDWE)联合艾瑞昔布片(IT)对神经根型颈椎病(CSR)疼痛程度及生活质量的影响。方法回顾性选取2021年12月至2023年7月浙江中医药大学附属丽水市中医院收治的CSR患者为研究对象。根据CSR患者治疗方案分为对照组(IT,0.1 g,po,bid,共4周)和联合组(IT,0.1 g,po,bid,共4周;畲药MDWE,熏蒸,40 g,每周6次,共4周)。比较两组CSR患者干预4周后疗效;治疗前和治疗2周后、4周后的视觉模拟疼痛评分(VAS),治疗前和治疗4周后的中医证候积分、血液流变学指标,治疗前和治疗3个月后的生活质量以及治疗期间的不良反应发生率。结果研究共纳入100例CSR患者,联合组52例,对照组48例。治疗4周后,联合组的治疗总有效率显著高于对照组(P<0.05)。治疗前,两组患者的VAS评分、颈部疼痛评分、运动障碍评分、肢体麻木评分、头晕评分、腰膝酸软评分、全血高切粘度、全血低切粘度、血浆粘度差异无统计学意义(P>0.05)。治疗2周后,两组CSR患者VAS评分较治疗前显著下降(P<0.05),且联合组患者VAS评分显著低于对照组(P<0.05)。治疗4周后,两组CSR患者上述评分和血液粘度均较治疗前显著下降(P<0.05),且联合组患者上述评分和血液粘度显著低于对照组(P<0.05)。治疗前,两组CSR患者生理职能、躯体疼痛、社会功能、精神健康、健康变化、生活质量总分差异无统计学意义(P>0.05);治疗3个月后,两组CSR患者上述评分均显著升高,且联合组上述评分高于对照组(P<0.05)。安全性方面,两组CSR患者治疗期间不良反应发生率差异无统计学意义(P>0.05)。结论与单一IT治疗比较,畲药MDWE联合IT治疗CSR患者可提高临床疗效、改善中医证候积分、降低血液粘度、改善生活质量,且不良反应小,值得临床推广应用。

不同基质对地菍扦插生根的影响

用细蛭石、粗蛭石、泥炭土和珍珠岩4种基质进行10种不同配比,以当年生未木质化地菍嫩枝为插穗进行扦插试验。采用根系分析仪对插穗生根部位进行形态学观察,并测定其生长状况,分析不同基质对地菍扦插生根的影响,结果表明:(1)4种基质等比混合的处理,地菍插穗的生根性状表现最好,生根率、长度、表面积、体积、根尖数及分叉数分别达89%、250.07 cm、42.94 cm^(2)、1.51 cm^(3)、568.13根和507.50个。(2)地菍扦插宜选用蛭石等质地疏松的材料为基质,避免选用泥炭土。(3)单因素试验中细蛭石和泥炭土扦插苗的生根率最高(100%),其次为粗蛭石(93.75%),珍珠岩基质生根率最低(81.25%)。(4)细蛭石扦插苗根的平均长度最大(301.56 cm),平均表面积最大(53.69 cm^(2)),平均根尖数最多(488.06根),平均分叉数最多(600.88个),且与其他基质扦插效果差异极显著(P<0.01)。透水、透气性较好的细蛭石有利于地菍扦插苗大规模生产。

The Melastoma dodecandrum genome and the evolution of Myrtales

Melastomataceae has abundant morphological diversity with high economic and ornamental merit in Myrtales. The phylogenetic position of Myrtales is still contested. Here, we report the chromosome-level genome assembly of Melastoma dodecandrum in Melastomataceae. The assembled genome size is299.81 Mb with a contig N50 value of 3.00 Mb. Genome evolution analysis indicated that M. dodecandrum,Eucalyptus grandis, and Punica granatum were clustered into a clade of Myrtales and formed a sister group with the ancestor of fabids and malvids. We found that M. dodecandrum experienced four whole-genome polyploidization events: the ancient event was shared with most eudicots, one event was shared with Myrtales, and the other two events were unique to M. dodecandrum. Moreover, we identified MADS-box genes and found that the AP1-like genes expanded, and AP3-like genes might have undergone subfunctionalization. The SUAR63-like genes and AG-like genes showed different expression patterns in stamens, which may be associated with heteranthery. In addition, we found that LAZY1-like genes were involved in the negative regulation of stem branching development, which may be related to its creeping features. Our study sheds new light on the evolution of Melastomataceae and Myrtales, which provides a comprehensive genetic resource for future research.

基于Gash修正模型的野牡丹灌丛降雨截留模拟

冠层截留是森林降雨再分配过程的重要组成部分,其量化对研究生态系统的水分平衡和养分循环具有重要意义。近年来,许多研究运用不同方法来解释这一过程,并确定不同森林植被的适用模型,但对具有复杂结构的灌丛的冠层截留研究较少。通过实地观测收集气象、降雨组分等数据,分析野牡丹灌丛降雨组成特征,应用Gash修正模型进行冠层截留的模拟及验证,并进行模型敏感性分析。结果表明,Gash修正模型能够很好地模拟野牡丹灌丛对降雨的截留过程。测得穿透雨632.6 mm、茎干流118.2 mm,计算得冠层截留141.4 mm,分别占总降雨的70.9%、13.2%和15.8%。总降雨与穿透雨、茎干流均有显著的线性相关性,表明穿透雨、茎干流随总降雨的增加而增加,而用二次函数拟合总降雨与冠层截留效果较理想(R^(2)=0.71)。对数方程能较好地解释穿透雨率、茎干流率、冠层截留率与总降雨量的相互关系。穿透雨、茎干流和冠层截留模拟分别比实测值高3.6%、低10.6%和7.3%。从模型模拟的各组成部分来看,冠层截留蒸发量占截留总量的44.4%,是截留的主要去向。敏感性分析表明:冠层郁闭度c、树干持水能力St的变化对Gash修正模型模拟冠层截留的影响最显著,而其他参数变化对该模型影响不明显。研究为湿润区结构复杂的灌丛降雨再分配研究提供了思路,有助于量化灌丛生态系统对局地水资源的影响。

民族药用植物地菍及其常见易混种的DNA条形码鉴定

目的:基于ITS2条形码序列对民族药用植物地菍Melastoma dodecandrum及其常见易混种进行分子鉴定,以保证药材使用的正确性和临床药效。方法:分别在浙江、江西、福建和广西等地采集地菍及其7种常见易混种多花野牡丹M.affine、野牡丹M.candidum、展毛野牡丹M.normale、毛菍M.sanguineum、少花柏拉木Blastus pauciflorus、锦香草Phyllagathis cavaleriei和秀丽野海棠Bredia amoena样品95份,提取DNA并进行PCR扩增,测序并获取ITS2序列,同时下载GenBank相应物种的ITS2序列30条。使用Gene Tool软件分析ITS2序列长度、GC含量和变异位点等情况,利用Clustal X和MEGA 7.0软件计算遗传距离和构建邻接(NJ)系统发育聚类树。结果:地菍及其常见易混种ITS2序列长度为224~236 bp,GC含量为60.6%~69.2%;地菍只有1种单倍型,种内K2P遗传距离显著小于其他7个易混种;邻接(NJ)进化树中地菍可与其他物种成功区分。结论:基于ITS2序列的DNA条形码技术能够成功鉴定地菍及其常见易混种,可为地菍的生产、使用和监管提供有力的安全保障。

套种地菍对油茶根系元素含量和土壤理化性质的影响

为研究套种地菍对油茶和土壤造成的影响,选取10年生油茶作为试验材料,在其林间种植地菍,以油茶的地下部分干鲜质量,油茶根部碳、氮、磷元素含量,土壤的pH、导电率、毛管孔隙度、容重、含水率和土壤的碳、氮、铁、铝、钾等13种元素含量作为测定指标,测定种植和未种植地菍的油茶林各指标,结果显示(1)套种的油茶根部磷元素含量极显著高于未套种(P=0.0012);(2)套种的土壤含水率显著高于未套种(P=0.03);(3)套种的土壤铜元素含量显著高于未套种(P=0.0169),有效磷元素含量极显著高于未套种(P=0.0053);(4)套种地菍的油茶体内磷元素含量与土壤pH呈极显著负相关;土壤含水率与土壤毛管孔隙度呈显著正相关,与土壤氮元素含量呈显著负相关;土壤铜元素含量与土壤钾、铁、磷、有效磷元素含量和土壤毛管孔隙度呈显著正相关,与土壤锌元素含量呈显著负相关;土壤有效磷含量与土壤铜、钾和铁元素含量呈显著正相关。该研究表明,油茶林套种地菍会对油茶根部磷元素含量、土壤含水率、有效磷和铜元素含量产生显著或极显著影响,但这些指标对油茶生长产生的影响还有待进一步研究。

畲药地稔组方对急性缺血性脑卒中预后及炎症因子的影响

目的探讨畲药地稔组方治疗急性缺血性脑卒中(acute ischemic stroke,AIS)的疗效,及对卒中后炎症因子、神经功能恢复及90 d生活独立能力的影响。方法选取2020年1月至2022年1月符合纳入标准的AIS患者193例,随机分为2组,对照组采用常规治疗;观察组在常规治疗基础上加用畲药地稔组方。两组患者治疗后第14天检测TNF-α、IL-1β、IL-6的浓度、NIHSS评分及90 dmRS评分,评估畲药地稔组方治疗的有效性;比较两组患者早期神经功能恶化、症状性颅内出血及90 d死亡率,明确畲药地稔组方的安全性。结果两组患者治疗14 d后,观察组TNF-α、IL-1β、IL-6水平低于对照组,观察组NIHSS评分低于对照组[(6.07±3.38)分VS.(7.19±3.53)分],观察组患者90 d获得生活独立能力的比例(mRs评分≤2分)明显高于对照组(52.6%VS.36.7%,P<0.05)。安全性方面,两组患者在早期神经功能恶化(9.5%VS.11.2%)、症状性颅内出血(5.3%VS.6.1%)及90 d死亡率(1.0%VS.2.0%),差异无统计学意义(P>0.05)。结论畲药地稔组方在AIS后72 h内给药,有助于降低炎症因子的释放,提高AIS患者90 d生活独立能力和促进神经功能恢复,从而改善卒中预后,降低致残率。

Thrombocyte counts in mice after the administration of methanolic extract of Melastoma malabathricum

Objective:To investigate the effect of methanolic extract of Melastoma malabathricum(M.malabathricum)in thrombocyte counts in mice.Methods:Methanolic extract of M.malabathricum corresponding to 1.5 to 2 mg/10 g body weight in saline was administered to mices via oral route.Control group was given normal saline.Twenty five microlitres of blood were drawn at 0 h and thereafterat 1,2,4,8,12,20 h after dosing via tail bleeding technique.The thrombocytes were counted in the triple laminated middle 25 squares of the haemocytometer using light microscope.Results:The control group showed a moderate rise after the administration of saline whereas the M.malabathricum treated group shows significant rise within 2nd h in thrombocyte counts with an increment of 51.64%compared to baseline count.Conclusions:Based on the results,it can be concluded that M.malabathricum could be a potential remedy in treating thrombocytopenic condition.

3个野牡丹品种耐热性研究

为探究紫绮、紫姝和霓裳3个优良野牡丹品种的耐热能力,以3个野牡丹品种叶片为材料,分析其表皮形态,在室温、35、40、45、50、55、60和65℃的水浴下加热15 min,测定其细胞伤害率,通过Logistic方程确定高温半致死温度,进行隶属函数分析。结果表明,霓裳、紫姝和紫绮的高温半致死温度分别为58.97、57.81和57.05℃,隶属函数综合值分别为2.436、2.305和1.184。综合考虑,3种野牡丹品种均具备一定的耐热能力,耐热性综合排序为霓裳>紫姝>紫绮。

NAA和IBA浸沾浓度及时间对地菍插枝发根的影响

地菍是集药用、观赏和果用为一体的新型多用途植物,人工栽培主要采用扦插育苗的方式,为促进地菍插枝的生根,研究了NAA和IBA两种物质不同浓度和浸泡时间的搭配对地菍不定根形成的影响。结果表明,NAA浓度在10~50mg/L时,浸泡时间在60~120s均可显著缩短发根初始时间、提高发根量和增加发根粗度和总根长,而IBA在100~200 mg/L,随着浓度和浸泡时间的增加,发根量也随之增加。

地稔不同插枝对根系生长及成活率的影响

地稔是集药用、观赏和果用为一体的新型多用途植物,人工栽培主要采用扦插育苗的方式,为促进地稔插枝的生根,研究了不同木质化程度和不同扦插长度对地稔成活和不定根形成的影响。结果表明,半木质化的插枝在发根量、根系总长、根系直径和成活率等方面均表现最佳,而未木质化的嫩枝根系发生时间虽然最早,但成活率却最低,高度木质化的插枝则根系生长最差;插枝长度以5~10 cm的发根量、总根长、根直径和成活率均最高,5 cm以下的扦插枝发根时间虽然最早,但根系直径最小、成活率也最低,10~15 cm的扦插枝则发根时间最晚、发根量和总根长最短。

基于正交试验的地菍扦插繁殖研究

以地菍(Melastoma dodecandrum)当年生半木质化枝条为插穗,蛭石为基质,采用正交试验和主成分分析,研究生根剂种类、生根剂浓度及处理时间的不同水平和处理组合条件下对地菍扦插生根效果的影响,筛选出适合地菍扦插繁殖的方案,旨在高效培育地菍幼苗,为地菍的扦插繁殖提供技术和理论的支撑。结果表明:地菍插穗以皮层生根和结上生根为主,不同处理组合的扦插生根率为40.00%~86.67%,平均根数为13.17~36.22条/株,平均根长为1.15~2.43 cm,最长根长为1.37~5.22 cm,生根效果指数为6.58~21.09,各处理间对地菍插穗生根影响均较大,且不同处理之间差异显著(P<0.05)。极差分析发现:生根剂浓度是影响地菍平均根长和最长根长的主要因素;生根剂种类是影响地菍平均生根数、生根效果指数的主要因素;影响地菍生根率的主导因子为生根剂处理时间。主成分分析联合生根效果指数指标极差分析结果得到,500 mg/L NAA处理0.5 min和100 mg/L IBA处理30 min是较适合地菍插穗生根的组合。

干旱胁迫及复水对野牡丹光合和叶绿素荧光参数的影响

为探究野牡丹的耐旱能力,以野牡丹(Melastoma candidum D.Don)为材料,采用盆栽模拟干旱的方法,持续干旱12 d后复水处理,分别在干旱第3、6、9、12天测量数据,以干旱0 d为对照组,对比不同程度干旱胁迫下及复水后野牡丹光合和叶绿素荧光参数的变化。结果表明,随着干旱程度的不断增加,植物净光合速率(Pn)、气孔导度(g_(s))、胞间CO_(2)浓度(C_(i))以及蒸腾速率(T_(r))降低,水分利用效率(WUE)先上升后下降;植物叶片最大光化学效率(F_(v)/F_(m))、光化学淬灭系数(qP)、光合电子传递速率(ETR)降低,非光化学淬灭系数(NPQ)有所上升,各项指标经过复水处理后均能恢复到干旱第3天水平。从野牡丹光合和叶绿素荧光参数随不同干旱胁迫的变化及复水后各项指标均能有所恢复可知,野牡丹具有一定的耐旱性。