The low abundance and highly hydrophobic nature of most membrane proteins make their analysis more difficult than that for common soluble proteins.Successful membrane protein identification is largely dependent on the...The low abundance and highly hydrophobic nature of most membrane proteins make their analysis more difficult than that for common soluble proteins.Successful membrane protein identification is largely dependent on the sample preparation including the enrichment and dissolution of the membrane proteins.A series of conventional and newly developed methods has been applied to the enrichment of low-abundance membrane proteins at membrane and/or protein levels and to the dissolution of hydrophobic membrane proteins.However,all the existing methods have inherent advantages and limitations.Up to now,there has been no unique method that can universally be employed to solve all the problems and more efforts are needed in improving sample preparation for the analysis of membrane proteomes.展开更多
The complications of hemodialysis accompanied the hemodialysis and threaten the patients’life.Besides the loss of nutrient substance,such as amino acid and vitamin,we found new clues that the adsorbed proteins on com...The complications of hemodialysis accompanied the hemodialysis and threaten the patients’life.Besides the loss of nutrient substance,such as amino acid and vitamin,we found new clues that the adsorbed proteins on common-used polysulfone-based dialysis membrane might be the reason according to the qualitative proteomic study by ionic liquid assisted sample preparation method.Our results indicated that the adsorbed proteins on the membrane were related with complement activation,blood coagulation,and leukocyte-related biological process.The quantitative proteome further demonstrated some significant changes of signal proteins in the post-dialysis plasma after the hemodialysis,such as beta-2-microglobulin and platelet factor-4,which would further verify these new clues.展开更多
基金supported by grants from Hunan Provincial Natural Science Foundation of China(No.11JJ2019)National Basic Research Program of China(No.2010CB529800)+1 种基金National Natural Science Foundation of China(Grant No.31070700)Specialized Research Foundation for the doctorial Program of Higher Education of China(No.20094306110003).
文摘The low abundance and highly hydrophobic nature of most membrane proteins make their analysis more difficult than that for common soluble proteins.Successful membrane protein identification is largely dependent on the sample preparation including the enrichment and dissolution of the membrane proteins.A series of conventional and newly developed methods has been applied to the enrichment of low-abundance membrane proteins at membrane and/or protein levels and to the dissolution of hydrophobic membrane proteins.However,all the existing methods have inherent advantages and limitations.Up to now,there has been no unique method that can universally be employed to solve all the problems and more efforts are needed in improving sample preparation for the analysis of membrane proteomes.
基金supported by the National Key Research and Development Program of China (2017YFA0505003,2016YFA0501401)the National Natural Science Foundation of China (21375126, 21405154)
文摘The complications of hemodialysis accompanied the hemodialysis and threaten the patients’life.Besides the loss of nutrient substance,such as amino acid and vitamin,we found new clues that the adsorbed proteins on common-used polysulfone-based dialysis membrane might be the reason according to the qualitative proteomic study by ionic liquid assisted sample preparation method.Our results indicated that the adsorbed proteins on the membrane were related with complement activation,blood coagulation,and leukocyte-related biological process.The quantitative proteome further demonstrated some significant changes of signal proteins in the post-dialysis plasma after the hemodialysis,such as beta-2-microglobulin and platelet factor-4,which would further verify these new clues.
