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MEN1在消化系统肿瘤中的研究进展
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作者 凡浩然(综述) 王震(审校) 《安徽医科大学学报》 CAS 北大核心 2024年第4期726-736,共11页
多发性内分泌腺肿瘤型1(MEN1)基因通过编码Menin调节细胞增殖和凋亡等过程,并且参与多个信号通路的调节,包括Wnt/β-catenin、Notch、Hedgehog/Dnmt1、Hippo等信号通路,这些都与肿瘤的发生、发展密切相关。因此,MEN1的突变或功能缺陷可... 多发性内分泌腺肿瘤型1(MEN1)基因通过编码Menin调节细胞增殖和凋亡等过程,并且参与多个信号通路的调节,包括Wnt/β-catenin、Notch、Hedgehog/Dnmt1、Hippo等信号通路,这些都与肿瘤的发生、发展密切相关。因此,MEN1的突变或功能缺陷可能导致肿瘤发生的风险增加。以往关于MEN1的研究主要集中于神经内分泌肿瘤,MEN1在神经内分泌肿瘤中作为抑癌基因发挥作用,其缺失突变引起的基因功能丧失,参与了神经内分泌肿瘤的发生发展。但近年研究表明,MEN1在不同的肿瘤中发挥不同的作用,如在胃癌中通过抑制IQ基元GTP酶激活蛋白1(IQGAP1)表现为抑癌基因,在肝癌中则通过甲基化相关途径表现为癌基因,在结直肠癌中通过自噬相关机制也表现为癌基因;而在食管癌、胰腺癌中MEN1的作用尚存在争议。现就近年来MEN1在消化系统肿瘤中的研究进展做一综述。 展开更多
关键词 men1 menIN 基因突变 促癌基因 抑癌基因 消化系统肿瘤 组蛋白修饰 生物信息分析
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MEN1基因在前列腺癌中生物学功能的研究进展
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作者 石庆 王子琦 罗亚坤 《现代肿瘤医学》 CAS 2024年第17期3379-3384,共6页
近二十年来,我国前列腺癌的发病率逐年攀升,已经成为泌尿外科三大恶性肿瘤中最常见的一种。当下我国前列腺癌患者中,初诊即晚期的比例相比于欧美日等发达国家仍然较高,且有超过70%的晚期前列腺癌病患发生转移,若出现远处转移,其患者生... 近二十年来,我国前列腺癌的发病率逐年攀升,已经成为泌尿外科三大恶性肿瘤中最常见的一种。当下我国前列腺癌患者中,初诊即晚期的比例相比于欧美日等发达国家仍然较高,且有超过70%的晚期前列腺癌病患发生转移,若出现远处转移,其患者生存率仅为30%。因此,进一步寻找新的诊疗分子靶点就具有更加重要的现实意义和理论价值。研究证实多发性内分泌肿瘤1型(MEN1)基因编码的menin蛋白参与前列腺癌的发生发展,同时menin是前列腺治疗潜在的新靶点。该文将对MEN1基因结构及menin在前列腺癌中的生物学功能进行概述,重点探讨menin调控前列腺癌细胞增殖、迁移与侵袭的分子机理。以期为MEN1基因及menin蛋白在前列腺癌中的深入研究提供参考。 展开更多
关键词 前列腺癌 men1基因 分子机制 治疗策略
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Epidemiological Profile of Men Who Have Sex with Men: First Data about Male Sex Workers in Central African Republic (CAR)
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作者 Hermione Dahlia Mossoro-Kpinde Balekouzou Augustin +1 位作者 Paola Julienne Demba Henri Saint Calvaire Diemer 《Advances in Sexual Medicine》 2024年第1期1-11,共11页
Objectives: Sex work is not well documented among African men. The aim of this study was to describe the epidemiological profile of men who have sex with men (MSM) and eventually to determine the proportion of sex wor... Objectives: Sex work is not well documented among African men. The aim of this study was to describe the epidemiological profile of men who have sex with men (MSM) and eventually to determine the proportion of sex workers (SW) among them, as well as the proportion of MSM who have been victims of gender-based violence (GBV). Material and Methods: A cross-sectional study coordinated by the Direction de la Médecine du Travail was carried out at the headquarters of the association ALTERNATIVES in Bangui Bangui from July 1 to October 31, 2021. Consenting MSM present during the study period were systematically included. Sociodemographic variables, those relating to the future vision of the activity and to GBV were collected and analyzed using Epi-Info version 7 software. Results: Forty MSM with an average age of 23 years and mainly secondary education (75.0%) were included. The vast majority of MSM were unemployed (85.0%). 45.0% had been victims of GBV and wanted to stop working as an MSM (47.5%), and almost 2/3 (65.0%) would accept another income-generating activity in exchange for the MSM. Violence was sexual (32.5%), economic (22.5%), physical (20.0%), verbal (12.5%) and psychological (12.5%). Conclusion: The MSM were mainly young, poorly educated, unemployed, and almost half were victims of GBV. The desire to change MSM activity to another income-generating activity alongside that of MSM shows that many of them are SW, workers in the informal sector. This must be taken into account in prevention activities, even if the data needs to be confirmed on a much larger sample. 展开更多
关键词 men Who Have Sex with men Sex Workers Gender-Based Violence Informal Sector Central Africa
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Urethroplasty among Elderly Men, Surgical Techniques and Outcomes
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作者 Kwaku Addai Arhin Appiah George Amoah +7 位作者 Patrick Opoku Manu Maison Roland Azorliade Kwaku Otu-Boateng Douglas Arthur Dominic Annor Mintah Joseph Yorke George Asafu Adjaye Frimpong Christian Kofi Gyasi-Sarpong 《Open Journal of Urology》 2024年第3期179-187,共9页
Introduction: Urethroplasty remains the gold standard for the management of urethral stricture. However, the treatment of stricture disease in the elderly tends to be less invasive due to the presumption that they mig... Introduction: Urethroplasty remains the gold standard for the management of urethral stricture. However, the treatment of stricture disease in the elderly tends to be less invasive due to the presumption that they might not be able to stand long hours of surgery and might have higher rates of recurrence due to poor wound healing from microangiopathy. We present our experience with the outcomes of urethroplasty among elderly men seen at the Komfo Anokye Teaching Hospital from January 2012 to December 2021. Methods: This was a retrospective review of data captured in the urology database on all patients 65 years and above who underwent urethroplasty at the hospital over the study period. Data was obtained on patients’ demographics, stricture characteristics, urethroplasty technique, and outcome. A successful outcome was defined as peak flow rate > 15 mls/s, a patent urethra on retrograde urethrogram, patient satisfaction with urine stream, or restoration of the normal stream of urine with only one attempt at urethral calibration or internal urethrotomy postoperatively. Data was analyzed using PASW Statistics for Windows, Version 18.0. Results: Overall, 43 urethroplasties were done over the study period in elderly men. The age range was 65 to 87 years. The commonest aetiology was catheterization (62.79%) followed by urethritis (32.56%). Stricture length ranged from 0.5 cm to 16 cm with a mean of 3.93 cm. Most patients (60.46%) had bulbar urethral strictures. The repair methods employed were anastomotic urethroplasty (62.80%), fasciocutaneous flap (FCF) ventral onlay (13.95%), buccal mucosa graft (BMG) ventral onlay urethroplasty (4.65%), and staged urethroplasty (4.65%). Three of the patients (6.98%) had a combination of anastomotic and tissue transfer urethroplasty. The overall success rate was 88.37%. Complications included three surgical site infections, two urethral diverticula and one glans dehiscence. Conclusion: Elderly men tolerate urethroplasty well and the procedure should not be denied solely based on age. 展开更多
关键词 Urethral Stricture Elderly men URETHROPLASTY Surgical Techniques
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Structural Gender Inequality and Mental Health among Chinese Men and Women
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作者 Lei Yang Zhipeng Sun 《International Journal of Mental Health Promotion》 2023年第1期31-43,共13页
Little is known about the association between structural gender inequality and health in patriarchal China.This study employed a sample from the Chinese Women’s Social Status,consisting of 26,139 participants aged 18... Little is known about the association between structural gender inequality and health in patriarchal China.This study employed a sample from the Chinese Women’s Social Status,consisting of 26,139 participants aged 18 and 70 years(13,494 women and 12,645 men).Structural gender inequality was assessed at the macro-,meso-,and micro-levels.Mental health was measured by the summed scores of eight questions on depressive symptoms.Multilevel linear regression was applied for analysis.Results showed that total sex ratio at birth was associated with poorer mental health among women and men but sex ratio at birth of the second-born child predicted better mental health.Gender inequality at meso-level resulted in poorer mental health and gender inequality at micro-level was associated with poorer mental health both for men and women.Eliminating structural gender inequality promotes populations’mental health in China. 展开更多
关键词 Structural gender inequality PATRIARCHY mental health men and women social determinants
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To Explore the Relationship between Lower Extremity Vascular Sclerosis and Osteoporosis in Elderly Men with Type 2 Diabetes Mellitus
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作者 Silu Jiang Hao Wang +1 位作者 Fuxia Xie Li Xia 《Advances in Bioscience and Biotechnology》 2023年第11期457-464,共8页
Objective: To investigate the correlation between lower extremity vascular sclerosis and osteoporosis in elderly men with type 2 diabetes mellitus. Methods: A total of 359 elderly male patients with type II diabetes h... Objective: To investigate the correlation between lower extremity vascular sclerosis and osteoporosis in elderly men with type 2 diabetes mellitus. Methods: A total of 359 elderly male patients with type II diabetes hospitalized in the First Affiliated Hospital of Chongqing Medical University from January 2018 to June 2023 were retrospectively collected. According to the BMD (Bone Density Value), the patients were categorized into osteoporotic (T ≤ -2.5, n = 248) and non-osteoporotic groups (T > -2.5, n = 111). T test and Chi-square test were used to evaluate the differences in clinical data, biochemical markers and ABI between two groups. Multivariate logistic regression was used to analyze the risk factors of osteoporosis in elderly men with type 2 diabetes mellitus. Results: Compared with the non-osteoporotic group, the differences in diabetes course, systolic blood pressure, ABI, BMI, uric acid, triglyceride, and HDL in the osteoporotic group were statistically significant (P < 0.05). Logistic multivariate regression analysis showed that lower extremity vascular sclerosis was an independent risk factor for osteoporosis in elderly men with type 2 diabetes mellitus (P Conclusion: Atherosclerosis of the lower extremities in elderly men with T2DM is closely related to osteoporosis, and can lead to a decrease in bone mass, and an increase in osteoporosis. 展开更多
关键词 Elderly men with Type 2 Diabetes Mellitus Lower Extremity Atherosclerosis OSTEOPOROSIS
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Men’s Knowledge, Attitudes and Perceptions on Family Planning in Tourourou Village, Gogui Rural Commune, Nioro Circle, Kayes Region of Mali
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作者 Alou Samaké Sitapha Dembele +18 位作者 Mamadou Keita Cheickna Sylla Dramane Haidara Mamadou Haidara Mamadou Diallo Moussa Konaté Mariam Maiga Mohamed Saydi Agmed Elméhdi Elansari Djibril Kassogué Seydou Zana Dao Hawa Samaké Abdoulaye Nouhoun Coulibaly Brehima Samaké Coulibaly Moussa Mariko Seydou Abdoulaye Cissé Séma Kéita Siaka Amara Sanogo Seydou Fané 《Open Journal of Obstetrics and Gynecology》 2023年第3期662-680,共19页
Introduction: The involvement of men in reproductive health programmes and more specifically in family planning has always been of concern to the various development actors at both national and international levels. O... Introduction: The involvement of men in reproductive health programmes and more specifically in family planning has always been of concern to the various development actors at both national and international levels. Objective: To study the births, ideas and opinions of men on Family Planning in the village of Tourourou. Material and Methods: This was a prospective cross-sectional, descriptive and analytic study from February 1 to July 31, 2020 in the village of TOUROUROU on the Knowledge, Attitudes and Perceptions of Men on Family Planning. We included in our study, all men residing in TOUROUROU whose age is between 14 and 99 years, who agreed to participate in the study. Results: Our study involved 200 men. The 25 - 29 age group was the most represented with 25.5%. The Soninke had represented 41%. In our study, 97.5% of men had heard of family planning, 62% had spontaneously cited at least one modern contraceptive method, and the pill ranked first with 64.5%. Radio and friends were the most frequent sources of information with 57.5% and 54.5% respectively. Birth spacing was the most cited family planning goal by men in 43.5% of cases. Men supported the practice of family planning in 50.5% of cases. Conclusion: In the village of Tourourou men play a crucial role in decision-making concerning the health of the couple and the family, their effective involvement in Reproductive Health especially in Family Planning will contribute enormously to the improvement of the health of the family. 展开更多
关键词 KNOWLEDGE ATTITUDES PERCEPTIONS men Planning Family
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Comparison of“Song to the Men of England”With“Large Rat”of Book of Songs From the Approach of New Criticism
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作者 HUANG Xiaoli 《Psychology Research》 2023年第12期604-608,共5页
New Criticism is an important theory in deciphering poetry.With the help of New Criticism,the meanings and the charms of the poetry are shown.“Song to the Men of England”and“Large Rat”are from different countries ... New Criticism is an important theory in deciphering poetry.With the help of New Criticism,the meanings and the charms of the poetry are shown.“Song to the Men of England”and“Large Rat”are from different countries with the same emotions of accusing the cruelty of the exploiter and the miserable life of the exploited.However,“Song to the Men of England”is full of hatred to the exploiter,hoping to destroy the society.Nevertheless,the exploited of“Large Rat”keep the hope of moving to a new free land.The two poems show different characteristics of Englishmen and Chinese. 展开更多
关键词 “Song to the men of England” “Large Rat” New Criticism
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MEN1基因与胃肠道胰腺神经内分泌肿瘤 被引量:4
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作者 叶必星 林琳 《国际消化病杂志》 CAS 2010年第6期365-367,379,共4页
MEN1基因为多发性内分泌腺肿瘤(MEN1)的易感基因,在调节基因的转录、维持基因的稳定、调控细胞的分裂和增殖方面起重要作用,而MEN1基因失活可能与胃肠道胰腺神经内分泌肿瘤(GEP-NET)发生机制有关。
关键词 胃肠道胰腺神经内分泌肿瘤 men1基因 menIN 分子机制
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Total Energy Expenditure of 16 Chinese Young Men Measured by the Doubly Labeled Water Method 被引量:4
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作者 ZHUO Qin SUN Rui +5 位作者 GOU Ling Yan PIAO Jian Hua LIU Jian Min TIAN Yuan ZHANG Yu Hui YANG Xiao Guang 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2013年第6期413-420,共8页
Objective Doubly labeled water (DLW) method is the gold standard for measuring total energy expenditure (TEE). We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±... Objective Doubly labeled water (DLW) method is the gold standard for measuring total energy expenditure (TEE). We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±1 recruited. TEE was measured by the DLW method, by indirect calorimetry. We also conducted 24-h estimate energy requirements of the subjects. years with body mass index 22.0±1.4 kg/m2 were and basal energy expenditure (BEE) was determined activity, energy balance and factorial approach to Results TEE of subjects by DLW method was 9.45±0.57 M J/day (2258±180 kcal/day). The 24-h activity was 10.80±0.33 M J/day (2582±136 kcal/day). The energy requirement, derived from energy balance observations, was 9.93±1.32 M J/day (2373±315 kcal/day). The BEE of 6.65±0.28 M J/day (1589±67 kcal/day), calculated by the adjusted Schofield equation, was significantly higher (P〈O.O01) than that measured by indirect calorimetry, 5.99-±0.66 M J/day (1433-±158 kcal/day). The TEE derived from the factorial approach was 10.31-±0.43 M J/day (2463±104 kcal/day). Conclusion The TEE of Chinese young men measured by the DLW method was about 10% lower than the current recommended nutrient intake (RNI), suggesting that the RNI for Chinese men maybe overestimated. Further studies are warranted to determine the value of the estimated energy requirement. 展开更多
关键词 Doubly labeled water Total energy expenditure Recommended nutrient intakes Estimatedenergy requirement Chinese young men
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Menin在翼状胬肉中的异常表达
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作者 王华 刘祖国 +3 位作者 夏晓波 刘双珍 赵丽蓓 王平宝 《中国现代医学杂志》 CAS CSCD 北大核心 2011年第19期2265-2268,共4页
目的探讨menin蛋白在初发性翼状胬肉组织中的表达。方法采用免疫组织化学染色和Western Blotting方法对30例初发性翼状胬肉组织进行menin蛋白的检测,并与26例正常人结膜组织进行对照。结果 Menin蛋白在正常人结膜上皮细胞和结膜基质血... 目的探讨menin蛋白在初发性翼状胬肉组织中的表达。方法采用免疫组织化学染色和Western Blotting方法对30例初发性翼状胬肉组织进行menin蛋白的检测,并与26例正常人结膜组织进行对照。结果 Menin蛋白在正常人结膜上皮细胞和结膜基质血管内皮细胞的细胞核内强阳性表达;Menin蛋白在翼状胬肉的结膜上皮细胞和基质血管内皮细胞的细胞浆内表达明显下调,弱阳性表达不再定位于细胞核内。结论 Menin蛋白表达异常可能在促进翼状胬肉上皮细胞和基质血管内皮细胞过度增殖中发挥重要的调控作用。 展开更多
关键词 menIN men1 翼状胬肉
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Development of a Social Activities Scale for Community-Dwelling Older Men Requiring Support in Japan 被引量:1
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作者 Michiyo Hirano Kazuko Saeki Izumi Ueda 《Health》 2018年第1期1-12,共12页
Aim: We developed a scale to measure the social activities of community-dwelling older men requiring support. Methods: The participants were a group of 134 men, ≥65 years old, who required support and were living in ... Aim: We developed a scale to measure the social activities of community-dwelling older men requiring support. Methods: The participants were a group of 134 men, ≥65 years old, who required support and were living in Hokkaido, Japan. An anonymous questionnaire was administered through individual interviews. Valid responses were obtained from 121/134 interviewees. The construct validity of the resulting scale was assessed by exploratory factor analysis (EFA). Criterion-related validity was tested with Spearman’s rank correlation test based on the Social Activities Index for Elderly People (SAI-E). Reliability was assessed by Cronbach’s alpha. Results: A Social Activities Scale for Community-dwelling Older Men Requiring Support (SASOMS) scale was created, comprised of the following three subscales: daily interactions with familiar people;intimate relationships with family members;and interactions with others through activity programs (e.g., exercise, games, recreation, etc.). The created SASOMS scale correlated with the SAI-E (r = 0.557), and its criterion-related validity was confirmed. The alpha coefficient of the new scale was 0.791, and its internal consistency was confirmed. Conclusions: The reliability and validity of the developed SASOMS scale was confirmed, demonstrating that it can be used to assess social activities specifically in older men requiring support. Our results suggest that the scale can be used effectively by care providers who support older men requiring care. The usability of the SASOMS should continue to be improved, and it is necessary to verify its validity in longitudinal studies. 展开更多
关键词 Instrument DEVELOPmenT OLDER men’s Health SOCIAL ACTIVITIES SCALE
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Structure of Macro- and Trace Elements Status Observed in Residents of Magadan Town
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作者 Lugovaya Elena Stepanova Evgenia 《Journal of Life Sciences》 2014年第9期794-797,共4页
The authors aimed the study at examining macro- and trace elements content in elderly people of the 1 period of middle age (21-35) residing in Magadan town to assess the content of 25 trace elements in hair samples ... The authors aimed the study at examining macro- and trace elements content in elderly people of the 1 period of middle age (21-35) residing in Magadan town to assess the content of 25 trace elements in hair samples on individual and population levels by atom-emission spectrometry with inductively-bonded argon plasma. It turned out that the majority of surveyed subjects were characterized by deformation of element status demonstrated by excess and deficit of the basic essential elements of different degrees of manifestation. Deficit of Co (68% of surveyed people), Mg (67%), Ca (64%), Cu (47%), K (46%), Zn (44%), I (41%), Na (33%), P (27%) and Se (24%) was typical for female subjects as well as excess of Si (28%), Zn (22%) and P (20%). Male residents were characterized by deficit of Co (81%), Ca (66%), Mg (65%), I (50%), Cu (37%), K and Na (27%) and excess of Zn (41%). In individual cases it was noted excess concentration of nominally essential and toxic elements in both examined groups. They were: A1, As, Cd, Li, Ni, Pb, Sn, V and B. While analyzing of medians of chemical concentration it was stated reliable sex-related differences (P 〈 0.05): content of Ca, Mg and Mn was higher in hair samples of women, and content of As, B, Cd, Cr, K, Li, Na, P, Pb and V, on the contrary, was higher in male samples. 展开更多
关键词 Macro- and trace elements misbalance the North men women.
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[Co(3,3-tri)(men)Cl][ZnCl_4]和[Co(3,3-tri)(cmen)Cl][ZnCl_4]两体系配合物的合成及两经式异构体的晶体结构 被引量:1
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作者 毛明毅 薛赛凤 +5 位作者 陈铅 陶朱 祝黔江 罗绪强 周忠远 周向葛 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 北大核心 2002年第4期420-426,共7页
分别合成了 [Co(3, 3-tri)(men)Cl][ZnCl4]、[Co(3, 3-tri)(cmen)Cl][ZnCl4] (3, 3-tri = N-(3-胺基丙基)-1, 3-丙二胺,men = N-甲基乙二胺,cmen = 1, 2-二胺基-丙烷) 2体系的部分配合物异构体,用单晶 X-射线衍射分析方法解析了2异构体... 分别合成了 [Co(3, 3-tri)(men)Cl][ZnCl4]、[Co(3, 3-tri)(cmen)Cl][ZnCl4] (3, 3-tri = N-(3-胺基丙基)-1, 3-丙二胺,men = N-甲基乙二胺,cmen = 1, 2-二胺基-丙烷) 2体系的部分配合物异构体,用单晶 X-射线衍射分析方法解析了2异构体的晶体结构。其中 [Co(3, 3-tri)(men)Cl][ZnCl4] 体系的一异构体Ⅰ的化学简式为 CoCl(C9H27N5)ZnCl4,晶体属正交晶系,空间群 Pca21,a = 16.788(2),b = 7.964(1),c = 14.416(2) 牛琕 =1927.3(4) ?,Dc = 1.747 g/cm3,Z = 4,F(000) = 1032,Mr = 506.91,R = 0.0352,wR =0.0935;[Co(3, 3-tri)(cmen)Cl]2+ 体系的一异构体Ⅱ的化学简式为 CoCl(C9H27N5)ZnCl4稨2O, 晶体属三斜晶系,空间群 P ,a = 9.511(3), b = 9.972(3),c = 11.694(3) 牛琣 = 68.367(5),b = 85.196(6),?= 86.580(5),V = 1026.9(5)?3,Dc = 1.698 g/cm3,Z = 2,F(000) = 536,Mr = 524.92,R = 0.0494,wR = 0.1180。两异构体中 Co3+ 为六配位,晶胞中对映体比例均为1:1。在配合物异构体Ⅰ和Ⅱ中,三元胺以经式排布,三元胺配体(3, 3-tri)仲胺上的氢相对于Cl分别处于顺位(syn-)和反位(anti-);二元胺配体氮(或邻位碳)取代的胺基氮原子(N*)与三元胺配体中的仲氮原子分别处于对位(trans(N*))和邻位(cis(N*))。 展开更多
关键词 [Co(3 3-tri)(men)C1][ZnCl4] [Co(3 3-tri)(cmen)Cl][ZnCl4] 两经式异构体 钴(Ⅲ)配合物 晶体结构 三元胺配体
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玫瑰黄链霉菌Men-myco-93-63发酵液对黄瓜白粉病抗性的影响 被引量:13
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作者 郭敬华 孟庆芳 +4 位作者 李亚宁 杨文香 张汀 周锟 刘大群 《华北农学报》 CSCD 北大核心 2007年第B08期1-4,共4页
药效试验表明,玫瑰黄链霉菌Men-myco-93-63发酵液对黄瓜白粉病有良好的防效,其中发酵液保护作用的防效为88.63%,治疗作用的防效为82.26%,表明该发酵液对黄瓜白粉病的防治既有保护作用又有治疗作用。通过对喷施发酵液后黄瓜叶片内的抗病... 药效试验表明,玫瑰黄链霉菌Men-myco-93-63发酵液对黄瓜白粉病有良好的防效,其中发酵液保护作用的防效为88.63%,治疗作用的防效为82.26%,表明该发酵液对黄瓜白粉病的防治既有保护作用又有治疗作用。通过对喷施发酵液后黄瓜叶片内的抗病相关酶的活性的测定发现,喷施Men-myco-93-63发酵液后黄瓜叶片内POD,CAT,PAL,PPO等酶的活性在一定时间内有明显上升的趋势。 展开更多
关键词 链霉菌men-myco-93-63 发酵液 黄瓜白粉病 酶活性
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玫瑰黄链霉菌Men-myco-93-63抗南方根结线虫相关酶活性及其防效 被引量:9
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作者 张艳杰 魏学军 +6 位作者 寇宏达 乔丹娜 冀红柳 李爱霞 赤国彤 李亚宁 刘大群 《西北农业学报》 CAS CSCD 北大核心 2013年第4期184-190,共7页
根据雌虫会阴花纹特征将采集地河北省定州市西南合村黄瓜棚的根结线虫鉴定为南方根结线虫,并研究对多种植物病原菌具有较强抑制作用的玫瑰黄链霉菌Men-myco-93-63对南方根结线虫的防效及其产生的与防治线虫相关的酶。结果表明,Men-myco-... 根据雌虫会阴花纹特征将采集地河北省定州市西南合村黄瓜棚的根结线虫鉴定为南方根结线虫,并研究对多种植物病原菌具有较强抑制作用的玫瑰黄链霉菌Men-myco-93-63对南方根结线虫的防效及其产生的与防治线虫相关的酶。结果表明,Men-myco-93-63发酵液对根结线虫二龄幼虫的校正致死率达到100%,粗酶液对线虫的校正致死率达到94.87%。Men-myco-93-63具有较强的胶原蛋白酶产生能力,也可产生丝氨酸蛋白酶和蛋白酶,发酵液中蛋白酶的活力达到473.363 7U/mL。推测胶原蛋白酶、蛋白酶、丝氨酸蛋白酶等酶类在该生防菌对南方根结线虫的防治中起重要作用。温室盆栽试验结果表明,玫瑰黄链霉菌Men-myco-93-63发酵液及其固体发酵物对根结线虫均有防效;其中,发酵液5倍稀释液对根结线虫的防效最好,达到39.99%。 展开更多
关键词 南方根结线虫 玫瑰黄链霉菌men-MYCO-93-63 蛋白酶 防治效果
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玫瑰黄链霉菌Men-myco-93-63产抗生素发酵培养基的优化 被引量:4
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作者 韩兴 刘亚南 +2 位作者 甄丹妹 刘大群 赤国彤 《江苏农业科学》 北大核心 2017年第10期100-103,共4页
以玫瑰黄链霉菌Men-myco-93-63为发酵菌株,采用Plackett-Burman试验设计、最陡爬坡试验、中心复合试验设计和响应面分析法,以抗生素产量为响应值,对其发酵培养基进行优化。首先,采用Plackett-Burman设计筛选出具有正显著效应的花生饼粉... 以玫瑰黄链霉菌Men-myco-93-63为发酵菌株,采用Plackett-Burman试验设计、最陡爬坡试验、中心复合试验设计和响应面分析法,以抗生素产量为响应值,对其发酵培养基进行优化。首先,采用Plackett-Burman设计筛选出具有正显著效应的花生饼粉和玉米浆。其次,通过最陡爬坡试验逼近最大响应区域。最后,采用中心复合试验对这2个因素进行优化。最终确定发酵的最适培养基含量配方为葡萄糖2%,可溶性淀粉1%,花生饼粉4.96%,玉米浆2.66%,氯化钠0.4%,碳酸钙0.3%,磷酸二氢钾0.02%。优化后发酵培养基抗生素产量为2 030.6 mg/L,比优化前的771.9 mg/L提高了1.63倍。 展开更多
关键词 玫瑰黄链霉菌men—myco-93—63 发酵 抗生素 响应面分析
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嗜铬细胞瘤SDHD和MEN1基因突变与杂合性缺失研究 被引量:1
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作者 孙海燕 苏颋为 +5 位作者 崔斌 姜蕾 李小英 宁光 张军妮 王卫庆 《上海交通大学学报(医学版)》 CAS CSCD 北大核心 2006年第1期22-24,共3页
目的探讨抑癌基因SDHD和MEN1的突变与杂合性缺失,在嗜铬细胞瘤(PCC)发病机制中的作用。方法采用DNA直接测序法对SDHD和MEN1基因的全部外显子及其侧翼进行分析,寻找基因突变。选用荧光标记11q13区-23区的8个多态性微卫星位点对37例PCC进... 目的探讨抑癌基因SDHD和MEN1的突变与杂合性缺失,在嗜铬细胞瘤(PCC)发病机制中的作用。方法采用DNA直接测序法对SDHD和MEN1基因的全部外显子及其侧翼进行分析,寻找基因突变。选用荧光标记11q13区-23区的8个多态性微卫星位点对37例PCC进行杂合性缺失频率检测。分析上述检测结果与临床资料的关系。结果在37例PCC肿瘤组织中未发现SDHD和MEN1基因突变。发现3种SDHD基因内含子改变(15519972c/g,15521745c/t,15520001c/a),2种MEN1基因内含子改变(9879384a/g,9878352C/T)。SDHD所在区域11q23的杂合性缺失频率为30.8%。MEN1所在区域11q13的杂合性缺失频率为26.9%。结论PCC中未发现SDHD和MEN1基因突变;发现一定比例的11q13区-23区域的杂合性缺失,提示在该区域可能存在其他的抑癌基因参与PCC的发病机制。 展开更多
关键词 SDHD men1 杂合性缺失 突变 嗜铬细胞瘤
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玫瑰黄链霉菌Men-myco-93-63营养型片剂的研制及应用 被引量:5
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作者 冯龙 魏娇洋 +4 位作者 陈英化 李亚宁 张汀 刘大群 赤国彤 《河北农业大学学报》 CAS CSCD 北大核心 2014年第5期77-81,88,共6页
为了研制出一种防治棉花黄萎病的玫瑰黄链霉菌Men-myco-93-63营养型片剂,本试验利用单因素和双因素方差分析法筛选其氮源、碳源和粘合剂,并在田间试验评价该片剂对棉花黄萎病的防效。结果表明:片剂中载体硅藻土与玫瑰黄链霉菌Men-myco-9... 为了研制出一种防治棉花黄萎病的玫瑰黄链霉菌Men-myco-93-63营养型片剂,本试验利用单因素和双因素方差分析法筛选其氮源、碳源和粘合剂,并在田间试验评价该片剂对棉花黄萎病的防效。结果表明:片剂中载体硅藻土与玫瑰黄链霉菌Men-myco-93-63种子液最适质量体积比为2∶1,碳源为蔗糖,浓度15%;氮源为花生饼粉,浓度为15%;粘合剂聚乙二醇6000或玉米淀粉。制备的片剂重量差异符合标准,含菌量3×107cfu/g,含水量2%,杂菌率为1%,4℃真空条件下保存120d,菌含量仍能达到2×105 cfu/g。Men-myco-93-63片剂1.0g/株对棉花黄萎病的防效达到88.66%,皮棉产量达到2245.50kg/km2,明显优于其它对照药剂。生物农药片剂作为一种新农药剂型,具有很高的应用潜力。 展开更多
关键词 玫瑰黄链霉菌men-MYCO-93-63 片剂 双因素方差分析法 田间试验
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玫瑰黄链霉菌Men-myco-93-63负调控基因nsdAmgh的克隆及序列分析 被引量:4
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作者 沈凤英 刘力强 +2 位作者 吴伟刚 李亚宁 刘大群 《华北农学报》 CSCD 北大核心 2009年第5期77-80,共4页
nsdA(Negative regulator of Streptomyces differentiation)基因是天蓝色链霉菌中发现的与抗生素合成相关的负调控基因。根据天蓝色链霉菌A3(2)的序列设计引物扩增玫瑰黄链霉菌Men-myco-93-63菌株中的该基因,经测序和序列分析表明,由... nsdA(Negative regulator of Streptomyces differentiation)基因是天蓝色链霉菌中发现的与抗生素合成相关的负调控基因。根据天蓝色链霉菌A3(2)的序列设计引物扩增玫瑰黄链霉菌Men-myco-93-63菌株中的该基因,经测序和序列分析表明,由保守序列引物nsdA-2R和nsdA-2L克隆到的Men-myco-93-63菌株中的该基因800 bp片段与已知nsdA基因核苷酸保守区域序列同源性达到99%,由全长序列扩增引物nsdA WZ-R和nsdA WZ-L克隆到的该基因包含一个完整的开放阅读框,共编码369个氨基酸,与变铅青链霉菌和天蓝色链霉菌A3(2)中的nsdA基因的核苷酸序列同源性达到100%,氨基酸序列同源性达到99%。该全长基因命名为nsdAmgh,其在玫瑰黄链霉菌Men-myco-93-63中的发现为阻断该负调控基因以期获得抗生素高产工程菌株提供了重要依据。 展开更多
关键词 玫瑰黄链霉菌men-MYCO-93-63 nsdA基因 克隆 序列分析
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