目的:了解男男同性恋(men who have sex with men,MSM)艾滋病原发性耐药及耐药基因突变位点现状。方法:检索CNKI,VIP,CBM,万方数据库,Pub Med,Web of Knowledge,Springer及Medline数据库,提取HIV耐药以及耐药基因突变位点数据,对效应量...目的:了解男男同性恋(men who have sex with men,MSM)艾滋病原发性耐药及耐药基因突变位点现状。方法:检索CNKI,VIP,CBM,万方数据库,Pub Med,Web of Knowledge,Springer及Medline数据库,提取HIV耐药以及耐药基因突变位点数据,对效应量进行合并,并对样本量、地区、人种、质量评价得分及采样时间进行亚组分析。结果:共纳入43篇文献,MSM未治疗HIV感染者总耐药率、蛋白酶抑制剂(proteinase inhibitors,PI)耐药率、核苷类反转录酶抑制剂(nucleoside reverse transcriptase inhibitors,NRTI)耐药率、非核苷类反转录酶抑制剂(non nucleoside reverse transcriptase inhibitors,NNRTI)耐药率分别为10.21%(95%CI:8.65%~12.03%),2.98%(95%CI:2.25%~3.93%),4.05%(95%CI:3.14%~5.21%),4.42%(95%CI:3.31%~5.88%)。PI主要突变位点、PI次要突变位点、NRTI突变位点、NNRTI突变位点的突变位点频数≥3的突变率分别为0.55%(95%CI:0.38%~0.80%),1.31%(95%CI:0.98%~1.75%),0.85%(95%CI:0.51%~1.40%),1.19%(95%CI:0.70%~2.01%);频数<3的突变率分别为0.79%(95%CI:0.55%~1.13%),1.73%(95%CI:1.21%~2.46%),0.86%(95%CI:0.61%~1.21%),2.24%(95%CI:1.77%~2.83%)。样本量、地区及人种均是异质性的来源,耐药率和基因突变位点突变率在不同的亚组中分布不同。结论:美洲、欧洲地区MSM原发性耐药率较高,亚洲地区较以往也有所升高;同时PI次要突变位点突变率亦较高,应引起重视。展开更多
目的了解北京市部分男男同性恋(men who have sex with men,MSM)人群HIV-1的分子流行情况,监测该地区人群HIV毒株的最新流行情况。方法提取17例男男同性恋者HIV-1抗体阳性样本全血基因组DNA,直接进行巢式PCR扩增HIVenv基因C2-V5区及gag...目的了解北京市部分男男同性恋(men who have sex with men,MSM)人群HIV-1的分子流行情况,监测该地区人群HIV毒株的最新流行情况。方法提取17例男男同性恋者HIV-1抗体阳性样本全血基因组DNA,直接进行巢式PCR扩增HIVenv基因C2-V5区及gag基因P17-P24区,对PCR产物进行核苷酸序列测定和分析,确定基因亚型。结果17例患者中,共存在CRF01-AE、B亚型和CRF07-BC3种亚型,其中CRF01-AE9例(52.94%),B亚型5例(29.41%),CRF07-BC3例(17.65%)。结论小样本量的流行病学调查显示,北京地区部分男男同性恋人群中主要存在CRF01-AE、B和CRF07-BC3种亚型;CRF01-AE已取代B亚型而成为北京市MSM人群中HIV主要流行亚型,CRF07-BC重组亚型在北京市MSM人群中增长迅速。展开更多
We investigated the expression of TIMP1, TIMP2, SPARC, VCAN, and CLEC3B genes, encoded matricellular proteins with pleiotropic functions, and glucose intolerance in obese male subjects with normal and impaired glucose...We investigated the expression of TIMP1, TIMP2, SPARC, VCAN, and CLEC3B genes, encoded matricellular proteins with pleiotropic functions, and glucose intolerance in obese male subjects with normal and impaired glucose tolerance. The purpose of this study was to examine the association between the gene expressions and glucose intolerance in obesity. The results indicate that obesity leads to significant increase of TIMP1, TIMP2, E2F1 and CLEC3B gene expressions in subcutaneous adipose tissue, especially TIMP2 gene. However, more significant increase of the expression of TIMP1 and TIMP2 was found in adipose tissue of obese patients with glucose intolerance. No significant changes were found in the expression of VCAN and SPARC genes in adipose tissue of obese subjects with normal glucose tolerance but increased in the group of obese subjects with glucose intolerance. At the same time, the E2F1 and CLEC3B gene expressions were decreased in adipose tissue of obese patients with glucose intolerance. Results of this study provide evidence that changes in the expression of genes encoded TIMP1, TIMP2, VCAN, SPARC, E2F1 and CLEC3B in subcutaneous adipose tissue of obese individuals associate with glucose intolerance.展开更多
Patients with multiple endocrine neoplasia type 1(MEN1)mutations are predisposed to MEN1 syndrome affecting various endocrine cell lineages.Following its identification in the late 1990s,laboratories around the world,...Patients with multiple endocrine neoplasia type 1(MEN1)mutations are predisposed to MEN1 syndrome affecting various endocrine cell lineages.Following its identification in the late 1990s,laboratories around the world,including our own,used gene-targeting approaches in murine models to study the MEN1 gene and its related diseases.Subsequently,this field of research witnessed an upsurge in the use of Men1 mutant mouse models to dissect MEN1 functions.These studies led to unraveling the natural history of MEN disease,and highlighted cellular and molecular mechanisms underlying the development of the disease.In this review,we present the currently available data concerning the generation and characterization of Men1 mutant mouse models in connection with MEN1 syndrome.展开更多
文摘目的:了解男男同性恋(men who have sex with men,MSM)艾滋病原发性耐药及耐药基因突变位点现状。方法:检索CNKI,VIP,CBM,万方数据库,Pub Med,Web of Knowledge,Springer及Medline数据库,提取HIV耐药以及耐药基因突变位点数据,对效应量进行合并,并对样本量、地区、人种、质量评价得分及采样时间进行亚组分析。结果:共纳入43篇文献,MSM未治疗HIV感染者总耐药率、蛋白酶抑制剂(proteinase inhibitors,PI)耐药率、核苷类反转录酶抑制剂(nucleoside reverse transcriptase inhibitors,NRTI)耐药率、非核苷类反转录酶抑制剂(non nucleoside reverse transcriptase inhibitors,NNRTI)耐药率分别为10.21%(95%CI:8.65%~12.03%),2.98%(95%CI:2.25%~3.93%),4.05%(95%CI:3.14%~5.21%),4.42%(95%CI:3.31%~5.88%)。PI主要突变位点、PI次要突变位点、NRTI突变位点、NNRTI突变位点的突变位点频数≥3的突变率分别为0.55%(95%CI:0.38%~0.80%),1.31%(95%CI:0.98%~1.75%),0.85%(95%CI:0.51%~1.40%),1.19%(95%CI:0.70%~2.01%);频数<3的突变率分别为0.79%(95%CI:0.55%~1.13%),1.73%(95%CI:1.21%~2.46%),0.86%(95%CI:0.61%~1.21%),2.24%(95%CI:1.77%~2.83%)。样本量、地区及人种均是异质性的来源,耐药率和基因突变位点突变率在不同的亚组中分布不同。结论:美洲、欧洲地区MSM原发性耐药率较高,亚洲地区较以往也有所升高;同时PI次要突变位点突变率亦较高,应引起重视。
文摘目的了解北京市部分男男同性恋(men who have sex with men,MSM)人群HIV-1的分子流行情况,监测该地区人群HIV毒株的最新流行情况。方法提取17例男男同性恋者HIV-1抗体阳性样本全血基因组DNA,直接进行巢式PCR扩增HIVenv基因C2-V5区及gag基因P17-P24区,对PCR产物进行核苷酸序列测定和分析,确定基因亚型。结果17例患者中,共存在CRF01-AE、B亚型和CRF07-BC3种亚型,其中CRF01-AE9例(52.94%),B亚型5例(29.41%),CRF07-BC3例(17.65%)。结论小样本量的流行病学调查显示,北京地区部分男男同性恋人群中主要存在CRF01-AE、B和CRF07-BC3种亚型;CRF01-AE已取代B亚型而成为北京市MSM人群中HIV主要流行亚型,CRF07-BC重组亚型在北京市MSM人群中增长迅速。
文摘We investigated the expression of TIMP1, TIMP2, SPARC, VCAN, and CLEC3B genes, encoded matricellular proteins with pleiotropic functions, and glucose intolerance in obese male subjects with normal and impaired glucose tolerance. The purpose of this study was to examine the association between the gene expressions and glucose intolerance in obesity. The results indicate that obesity leads to significant increase of TIMP1, TIMP2, E2F1 and CLEC3B gene expressions in subcutaneous adipose tissue, especially TIMP2 gene. However, more significant increase of the expression of TIMP1 and TIMP2 was found in adipose tissue of obese patients with glucose intolerance. No significant changes were found in the expression of VCAN and SPARC genes in adipose tissue of obese subjects with normal glucose tolerance but increased in the group of obese subjects with glucose intolerance. At the same time, the E2F1 and CLEC3B gene expressions were decreased in adipose tissue of obese patients with glucose intolerance. Results of this study provide evidence that changes in the expression of genes encoded TIMP1, TIMP2, VCAN, SPARC, E2F1 and CLEC3B in subcutaneous adipose tissue of obese individuals associate with glucose intolerance.
基金the Fondation de l’Association pour la Recherche contre le Cancer(PJA 20151203335)SCUSI 2017 program from la Région Auvergne Rhône-Alpes and the grant from Ligue contre le Cancer inter-régionale 2018.
文摘Patients with multiple endocrine neoplasia type 1(MEN1)mutations are predisposed to MEN1 syndrome affecting various endocrine cell lineages.Following its identification in the late 1990s,laboratories around the world,including our own,used gene-targeting approaches in murine models to study the MEN1 gene and its related diseases.Subsequently,this field of research witnessed an upsurge in the use of Men1 mutant mouse models to dissect MEN1 functions.These studies led to unraveling the natural history of MEN disease,and highlighted cellular and molecular mechanisms underlying the development of the disease.In this review,we present the currently available data concerning the generation and characterization of Men1 mutant mouse models in connection with MEN1 syndrome.
