In order to investigate the effects of verapamil on the proliferation of meningiomas cells in vitro and in vivo, the cultured meningiomas cells were cultured with verapamil at different concentrations for 24 h and the...In order to investigate the effects of verapamil on the proliferation of meningiomas cells in vitro and in vivo, the cultured meningiomas cells were cultured with verapamil at different concentrations for 24 h and the inhibitory effects of verapamil on cell proliferation were observed by MTT method. The meningiomas model was established by implanting the newly removed tumor fragments into the nude mice subcutaneously. The nude mice with tumors were divided into two groups： verapamil-treated group and control group. Tumor volumes were measured and after 12 weeks the tumors were taken out and examined histologically. The expression of proliferating cell nuclear antigen （PCNA） in the tumors was detected by using immunohistochemistry. It was found that verapamil could inhibit the growth of cultured meningiomas cells in a concentration-dependant manner. The inhibitory effect could be observed in the concentration of 1 μmol/L verapamil and the most obvious effects appeared in the concentration of 100 μmol/L. Tumor volume in the verapamiltreated group was obviously smaller than that in the control group （211.40±5.50 vs 163.94±3.62, P〈0.01） and the expression of PCNA was also lower （1.52±0.24 vs 2.86±0.53, P〈0.05）. Tumor inhibition rate was about 22.45%. It was suggested that verapamil could inhibit the proliferation and growth of meningiomas cells in vitro and in vivo.展开更多
目的探讨维拉帕米对脑膜瘤细胞增殖的影响。方法将不同浓度维拉帕米作用于培养的脑膜瘤细胞,MTT法观察其对细胞增殖的抑制作用。建立裸鼠皮下脑膜瘤模型,观察服用维拉帕米后肿瘤的生长情况,免疫组织化学检测皮下肿瘤增殖细胞核抗原(PCNA...目的探讨维拉帕米对脑膜瘤细胞增殖的影响。方法将不同浓度维拉帕米作用于培养的脑膜瘤细胞,MTT法观察其对细胞增殖的抑制作用。建立裸鼠皮下脑膜瘤模型,观察服用维拉帕米后肿瘤的生长情况,免疫组织化学检测皮下肿瘤增殖细胞核抗原(PCNA)的表达。结果维拉帕米呈浓度依赖性地抑制脑膜瘤细胞增殖,浓度为1 μmol／L时即有抑制效应,抑制率为 16．82％,100μmol／L时抑制作用最明显,为52．95％。服药组皮下肿瘤体积明显小于对照组 (163．94±10．24 VS 211．40±16．51,P<0．01),其PCNA表达也低于对照组(1．52±0．24 VS 2．86±0．53,P<0．05)。结论维拉帕米在体外和体内均可抑制脑膜瘤细胞的增殖和生长。展开更多
文摘In order to investigate the effects of verapamil on the proliferation of meningiomas cells in vitro and in vivo, the cultured meningiomas cells were cultured with verapamil at different concentrations for 24 h and the inhibitory effects of verapamil on cell proliferation were observed by MTT method. The meningiomas model was established by implanting the newly removed tumor fragments into the nude mice subcutaneously. The nude mice with tumors were divided into two groups： verapamil-treated group and control group. Tumor volumes were measured and after 12 weeks the tumors were taken out and examined histologically. The expression of proliferating cell nuclear antigen （PCNA） in the tumors was detected by using immunohistochemistry. It was found that verapamil could inhibit the growth of cultured meningiomas cells in a concentration-dependant manner. The inhibitory effect could be observed in the concentration of 1 μmol/L verapamil and the most obvious effects appeared in the concentration of 100 μmol/L. Tumor volume in the verapamiltreated group was obviously smaller than that in the control group （211.40±5.50 vs 163.94±3.62, P〈0.01） and the expression of PCNA was also lower （1.52±0.24 vs 2.86±0.53, P〈0.05）. Tumor inhibition rate was about 22.45%. It was suggested that verapamil could inhibit the proliferation and growth of meningiomas cells in vitro and in vivo.
文摘目的探讨维拉帕米对脑膜瘤细胞增殖的影响。方法将不同浓度维拉帕米作用于培养的脑膜瘤细胞,MTT法观察其对细胞增殖的抑制作用。建立裸鼠皮下脑膜瘤模型,观察服用维拉帕米后肿瘤的生长情况,免疫组织化学检测皮下肿瘤增殖细胞核抗原(PCNA)的表达。结果维拉帕米呈浓度依赖性地抑制脑膜瘤细胞增殖,浓度为1 μmol／L时即有抑制效应,抑制率为 16．82％,100μmol／L时抑制作用最明显,为52．95％。服药组皮下肿瘤体积明显小于对照组 (163．94±10．24 VS 211．40±16．51,P<0．01),其PCNA表达也低于对照组(1．52±0．24 VS 2．86±0．53,P<0．05)。结论维拉帕米在体外和体内均可抑制脑膜瘤细胞的增殖和生长。
