基于区间毕达哥拉斯犹豫模糊熵和交叉熵的ELECTRE II决策方法

针对属性权重完全未知或部分已知,属性值为区间毕达哥拉斯犹豫模糊数的多属性决策问题,提出了基于区间毕达哥拉斯犹豫模糊熵和交叉熵的ELECTRE II法。首先给出区间毕达哥拉斯犹豫模糊数的区间形式的得分函数和精度函数,定义新的距离测度。然后基于区间毕达哥拉斯犹豫模糊数的模糊因子、直觉因子和幅度因子,给出熵和交叉熵公式,并证明其性质,提出了基于熵和交叉熵确定属性权重的方法。最后提出了区间毕达哥拉斯犹豫模糊环境下的改进的ELECTRE II法,利用综合优势值对方案进行排序,并通过算例和比较分析验证了该方法的可行性和有效性。

基于改进NSGA-II的轨道交通接驳公交线路优化

为解决接驳公交线路规划不合理和时间安排不完善的问题,提出了基于改进NSGA-II算法的环形接驳公交线路优化方法。首先,结合双层规划理论,以乘客出行时间成本最小化、公交企业运营收益和接驳公交服务率最大化为目标函数,以接驳公交线路站点数、线路长度和发车频率作为约束条件构建上层模型,采用Logit模型构建了下层接驳客流分配模型;其次,运用Floyd算法对NSGA-II算法的初始化种群进行了优化,针对所提出的模型设计了模型求解流程;最后,以哈尔滨市轨道交通1号线医大一院轨道交通站为案例,运用笔者提出的多目标双层规划模型和算法进行求解,并与原NSGA-II算法和基于Logistic混沌映射的NSGA-II算法进行对比。研究结果表明:基于Floyd算法改进的NSGA-II算法在多目标双层规划模型求解时,收敛速度更快效果更好,求解结果可以在Pareto前沿得到多个相互非支配的最优解;不同解集对应目标函数值不同,但可以达到接驳公交网络整体效益最优,采用折衷最优解集表述求解结果。

Synthesis and Characterization of Chiral Mercury(II) and Cadmium(II) Complexes with 3,3'-Bipyridine-5,5',6,6'-tetramethyl-2,2'-dimethoxy-1,1'-biphenyl

Two homochiral mercury （1） and cadmium （2） complexes derived from chiral twisted biphenyl pyridine ligand （3,3＇-bipyridine-5,5＇,6,6＇-tetramethyl-2,2＇-dimethoxy-1,1＇-biphenyl） have been synthesized and characterized by IR, microanalysis, TGA, UV-Vis, powder and single-crystal X-ray crystallography. Both 1 and 2 crystallize in orthorhombic space group P21212 with Z = 2. For 1, a = 14.2038（16）, b = 14.3630（17）, c = 7.0257（8）, V = 1433.3（3）3, Mr = 878.91, Dc = 2.037 g/cm3, μ = 7.549 mm-1, F（000） = 824, the final GOF = 1.017, R = 0.0296 and wR = 0.0645 for 2925 observed reflections with I 2σ（I）. For 2, a = 14.212（3）, b = 14.392（3）, c = 7.0498（14）, V = 1442.0（5）3, Mr = 790.72, Dc = 1.821 g/cm3, μ = 2.924 mm–1, F（000） = 760, the final GOF = 1.075, R = 0.0340 and wR = 0.0834 for 3144 observed reflections with I 2σ（I）. The crystal structures of 1 and 2 are isostructural and each adopts a two-dimensional supramolecular network which contains the C–H···π interactions.

融合NSGA-II和CSA的多目标车间调度

针对在灵活车间系统中调度作业和自动引导车(automated guide vehicle,AGV)的同时调度问题,考虑在有限多个AGV和加工机台的情况下,以最小化最大完工时间、单个AGV搬运消耗时间及所有AGV搬运总消耗时间为目标函数,设计融合NSGA-II(non-dominated sorting genetic algorithms)和克隆选择(clonal selection algorithm,CSA)的改进算法INGCSA来解决此类问题。采用工件、加工机台和AGV三部分编码;引入非支配排序和目标函数值大小排序后总得分进行种群分层,从而有效地保留优秀个体;针对克隆后的种群,对不同等级的种群采取不同的变异概率,并对染色体进行内部交换与均匀交叉混合交换的基因重组,有效地提高了种群的多样性与防止陷入局部最优。通过三组对比实验,验证了该算法在探索最优解时,具有运行时间短、稳定性高和收敛性好等优点。

营心宁胶囊辅助治疗对冠心病心力衰竭患者血管紧张肽II水平和心功能的影响

目的:观察营心宁胶囊辅助治疗对冠心病心力衰竭患者血管紧张肽II (AngII)水平和心功能的影响。方法:将128例患者分为两组,对照组64例,治疗组64例。对照组予常规治疗,治疗组患者在此基础上加用营心宁胶囊治疗,每次4粒,每日3次。治疗前及治疗8周后检测血浆AngII、脑钠肽(BNP),并行心脏彩色多普勒超声心动图测定左室射血分数(LVEF)、左室舒张末期内径(LVEDD)和每搏输出量(SV)。结果:治疗组和对照组总有效率分别为92.2%和89.1% (P > 0.01);显效率分别为43.8%,32.8% (P < 0.01)。两组治疗后患者的AngII、BNP、LVEF、LVEDD和SV均较治疗前明显改善(P < 0.01或P < 0.05),且治疗组患者的改善水平优于对照组(P < 0.01)。结论:营心宁胶囊能有效降低患者AngII水平,并对心功能有改善作用。

血管紧张素II对脓毒症休克大鼠心脏功能的影响

本研究拟通过盲肠结扎穿孔制备大鼠脓毒症模型,观察血管紧张素II对脓毒症休克(sepsis-shock)大鼠心脏功能的影响。方法 48只(Sprague-Dawley,SD)雄性大鼠随机分为6组(n=8):对照(control)组、假手术(sham)组、脓毒症休克(SS)组、去甲肾上腺素(NE)治疗组、血管紧张素II (ANGII)治疗组。采用盲肠结扎穿孔制备脓毒症休克动物模型。监测大鼠心率、血压变化。监测过程中大鼠如出现平均动脉压下降至65mmHg以下,立即给予0.9%生理盐水进行液体复苏,观察10分钟后如效果不佳,按照分组给予不同血管活性药物泵入。分别在造模后6,12,18,24小时抽血行心肌酶学指标检测,同时在该时段行超声观察大鼠心脏超声检查。于术后24小时留取心脏组织进行氧化因子指标测定,并进行心脏组织病理学观察。结果 与对照组和假手术组对比,脓毒症休克、去甲肾上腺素组、血管紧张素组平均动脉压下降明显,液体复苏效果欠佳,需要血管活性药物干预。与对照组和假手术组对比,脓毒症休克、去甲肾上腺素组、血管紧张素II组炎症反应、乳酸指标明显升高,具有统计学差异(P<0.05)。脓毒症休克组与对照组、假手术组相比,心脏左室射血分数(LVEF)明显下降,具有统计学差异(P<0.05),应用血管活性药物后,左室射血分数明显提高,血管紧张素II组射血分数提高与脓毒症组相比,具有显著统计学差异(P<0.01)。脓毒症休克组与对照组、假手术组相比,心肌酶学指标明显升高,应用去甲肾上腺素或血管紧张素II干预后,心肌酶学指标下降,与脓毒症组相比,具有统计学差异(P<0.05),血管紧张素II组改善更为明显(P<0.01)。结论 血管紧张素II可改善脓毒症休克对大鼠的心肌损害,对脓毒症休克大鼠心肌有一定的保护作用。

Isotherm, Kinetic and Thermodynamic Studies for the Sorption of Mercury (II) onto Activated Carbon from <i>Rosmarinus officinalis</i>Leaves

The present work deals with the equilibrium adsorption of Hg(II) onto carbonized Rosmarinus officinalis leaves (ACROL) as a new adsorbent from aqueous solution and it has been investigated. ACROL samples were prepared by physical carbonization at 773 K for 1 h. Titration method was used to determine the concentration of Hg(II) before and after adsorption onto ACROL by ethylenediaminetetraacetic acid, EDTA, as chelating agent. Batch equilibrium studies were carried out under different experimental conditions such as Hg(II) concentration and temperature. The relationship between the amount of Hg(II) onto ACROL can be described using four tow-parameter isotherm models. The equilibrium sorption data were analyzed using Freundlich, Langmuir, Dubinin-Radushkevich (DRK) and Temkin isotherms. The experimental results were found to fit the Langmuir isotherm model with a monolayer adsorption capacity of 588.2 mg/g at 318 K, while they were found to fit the Freundlich isotherm model at 298 K. The KL was decreased with increasing temperature, indicating a bond strength between Hg(II) and ACROL decreased with increasing temperature and sorption is exothermic. From DRK isotherm, free energy, E, was higher than 31 kJ/mol suggesting the Hg(II) adsorption onto ACROL chemical sorption. The thermodynamic studies revealed that the process is spontaneous nature of Hg(II) adsorption by ACROL and exothermic. The findings from this research show that ACROL has capability to remove Hg(II) from aqueous solutions.

表达Strep-tag II的重组塞内卡病毒的构建与鉴定

为构建表达Strep-tag II的重组塞内卡病毒(SVA),本研究在感染性cDNA克隆p SVA-I212V/S460L的基础上,采用重叠延伸PCR扩增Strep-tag II并采用无缝克隆法将其插入SVA VP1基因C端,构建感染性cDNA克隆,命名为p SVA-Strep,并采用双酶切和测序鉴定正确后,将p SVA-Strep转染BHK-21细胞拯救重组病毒,收集病变细胞上清,并将含有病毒的细胞上清和野生型SVA(SVA-WT)分别感染BHK-21细胞,以未感染病毒的BHK-21细胞作为阴性对照,感染后24 h分别以鼠源SVA VP2蛋白单克隆抗体(MAb)5D10(1∶1000)、鼠源Strep-tag II MAb(1∶2000)作为一抗,以FITC标记的山羊抗小鼠Ig G(1∶2000)为二抗,采用间接免疫荧光试验(IFA)对重组病毒鉴定。IFA结果显示,以鼠源SVA VP2蛋白MAb 5D10为一抗,SVA-WT和重组病毒感染的细胞均出现绿色荧光,以Strep-tag II MAb作为一抗时,仅有重组病毒感染的细胞出现绿色荧光,经SVA-WT感染的BHK-21细胞及阴性对照细胞均无绿色荧光,表明拯救了Strep-tag II标记的重组病毒,并将其命名为r SVA-Strep。将r SVA-Strep在BHK-21细胞中连续传10代,每隔2代采用引物VP1-F/R经PCR鉴定并对第10代重组病毒的VP1基因测序以鉴定r SVA-Strep的遗传稳定性。结果显示,每隔两代的重组病毒经PCR扩增后均出现约780 bp的目的条带,第10代重组病毒扩增的VP1基因测序结果显示Strep-tag II基因仍在重组病毒中,且无基因突变,表明r SVA-Strep的遗传稳定性较强;将SVA-WT和r SVA-Strep分别以MOI 0.01感染BHK-21细胞,在感染后4 h、8 h、12 h、24 h、36 h、48 h和72 h收获病毒测定病毒滴度并绘制生长曲线,结果显示在病毒感染36 h内r SVA-Strep和SVA-WT生长动力学基本一致,表明Strep-tag II的引入在病毒感染36 h内对SVA的复制基本无影响。采用0.2%甲醛充分灭活r SVA-Strep,并利用Strep-TactinRXT Purification纯化试剂盒纯化该灭活病毒,将r SVA-Strep灭活病毒上清加至Strep-Tactin亲和层析柱,分别用不同体积洗脱缓冲液依次洗脱病毒并通过western blot鉴定病毒的纯化效果。对纯化过程中不同洗脱液洗脱病毒的western blot结果显示,不同体积的洗脱液中E1、E2和E3均出现VP0和VP2两条带,且E1和E2的条带最明显,表明洗脱缓冲液的最佳洗脱体积约为2.2 CV;以上结果表明r SVA-Strep可通过Strep-Tactin亲和层析柱一步法纯化。本研究在SVA中插入Strep-tag II标签,为SVA灭活疫苗的快速纯化提供技术手段,也为其他病原的快速纯化方法的建立提供参考。

基于NSGA-II遗传算法的定轴注射模具成型工艺参数优化

为解决注射成型过程中铸件的质量和铸造效率低等问题,提出一种NSGA-II算法和TOPSIS方法与响应面法相结合的新型注射工艺参数优化筛选方法。以定轴为研究对象,采用Box-Behnken设计,以熔体温度、模具温度、注射时间、保压压力为变量,以翘曲变形量和体积收缩率为响应变量,采用NSGA-II遗传算法对2个响应的目标函数执行优化,用TOPSIS方法求解优化得到的Pareto前沿解集,找到最优解。仿真结果表明,当注射时间为40.71s、模具温度为131℃、熔体温度为180.07℃、保压压力为65 MPa时,翘曲变形降低了10.92%、体积收缩率降低了11.19%。优化后的注射工艺参数可有效消除铸件内部收缩松动和缩孔缺陷,形成性能良好的致密铸件,提高了产品质量。

Fe(II)改性蒙脱石对土壤汞的吸附固定机理探究

为明确Fe-MMT对土壤汞离子(Hg^(2+))的吸附、解吸特性及潜在作用机理,采用FeSO_(4)·7H_(2)O改性钠基蒙脱土(Na-MMT),合成了Fe(II)基蒙脱石(Fe-MMT),并探究了不同pH值、吸附剂用量、Hg^(2+)初始浓度和反应时间等条件下,Fe-MMT对土壤Hg^(2+)的吸附解吸特性、吸附动力学和等温吸附模型。结果发现:在pH=5、吸附剂用量为0.5 g、Hg^(2+)初始浓度为170 mg/L、反应时间为0.5 h的条件下,Fe-MMT对土壤Hg^(2+)的去除率高达76.8%;Fe-MMT对Hg^(2+)+的吸附过程符合Langmuir等温吸附模型,伪二阶动力学模型可较好地拟合吸附动力学行为,表明该吸附过程主要为单分子层吸附,且主要发生在吸附剂表面的均相反应位点上;结合微观结构表征,发现Fe-MMT对土壤Hg^(2+)的吸附主要通过-S^(2-)、-SH和-OH与Hg^(2+)进行络合,以及Fe^(2+)与Hg^(2+)的原位共沉淀络合引起的;Fe-MMT在水-土混合体系中的解吸率为9.7%,表明Fe-MMT对土壤Hg^(2+)具有较强的固定作用。可见,Fe-MMT具有修复Hg污染土壤的潜力。

Substituent Effect on Mercury-199 Chemical Shifts in Some Bisarylmercurials and Aryl(2-benzothiazolylthio) mercurials

The present paper covers the 199 Hg NMR chemical shifts of 24 substituted diphenylmercurials and phenyl(2-benzothiazolylthio)mercurials. There is a good linear relationship between the chemical shift and the Hammett constant of the substituents for both series of compounds, and electron donating substituents cause the chemical shift towards downfield.

Formation of Mercury(II)-Glutathione Conjugates Examined Using High Mass Accuracy Mass Spectrometry

Maternal exposure to Hg(II) during pregnancy has been identified as a potential causal factor in the development of severe neurobehavioral disorders. Children with autism have been identified with lower reduced glutathione (GSH)/oxidized glutathione (GSSG) ratios, and GSH is known to strongly bind Hg(II). In order to gain insight into the mechanism by which GSH binds Hg(II), high resolution mass spectrometry coupled with tandem mass spectrometry was utilized to examine the conjugation process. While the 1:1 Hg(II):GSH conjugate is not formed immediately upon mixing aqueous solutions of Hg(II) and GSH, two species containing Hg(II) are observed:the 1:2 Hg(II):GSH conjugate, [(GS)2 Hg + H+], and a second Hg(II)-containing species around m/z 544. Interestingly, this species at m/z 544 decreases in time while the presence of the 1:1 Hg(II):GSH conjugate increases, suggesting that m/z 544 is an intermediate in the formation of the 1:1 conjugate. Experiments using the high mass accuracy capability of Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry coupled to an electrospray ionization source indicate that the intermediate species is [GSH + HgCl]+, andnotthe 1:1 conjugate [Hg(GSH) – H + 2H2O]+postulated in previous literature. Further confirmation of [GSH + HgCl]+ is supported by collisionofinduced dissociation experiments, which show neutral loss of HCl from the intermediate and loss of the N- and C-terminal amino acids, indicating binding of Hg(II) at the Cys residue.

Effect of the Number of Donor Atoms in Calix[4]arene Derivatives for Selective Complexation and Extraction of Mercury(II) Cations, Thermodynamics and Extraction Studies

Water pollution due to gold mining activity is increasingly worrying. One way to selectively mitigate it is through the use of complexing agents such as calixarenes, which selectively functionalized with soft donor atoms such as sulphur, can selectively remove this pollutant, for the selective complexation of mercury from contaminated water, which was compared by conductimetry and liquid-liquid extraction studies with results that show that the derivative with the highest sulphur atoms is its structure which presents greater selectivity compared to the derivative with the lowest number of sulphur atoms, in both cases, they present very stable complexes.

Diagnostics of Optical Characteristics and Parameters of Gas-Discharge Plasma Based on Mercury Diiodide and Helium Mixture

The results of diagnostics of spectral, temporal and energy characteristics of the radiation of gas-discharge plasma in a mixture of mercury diiodide vapor with helium in the spectral range of 350 - 900 nm, and the plasma parameters in the range of reduced electric field E/N = 1 - 100 Td are presented. The plasma is created in the barrier discharge device with a cylindrical aperture. The electrodes are placed 0.2 m in length at a distance of 0.015 m. The amplitude of the pump pulse, the duration and frequency were 20 - 30 kV, 150 ns and 1 - 20 kHz, respectively. Radiation in the visible region of the spectrum of mercury monoiodide exciplex molecule is revealed. Regu larities in the optical characteristics of the plasma, depending on the partial pressures of the components of the mixture, the electron energy distribution function, mean electron energy, specific losses of discharge power on the process of dissociative excitation of mercury monoiodide (state B2Σ+1/2) molecules as well as the rate constant of dissociative excitation of mercury monoiodide molecules in working mixture depending on the given reduced electric field are established.

Processing of Mercury-Stibium Gold Bearing Ore ot Diiiikrut Deposit

The mercury-antimony-gold ore was subjected of the flotation. As a result of enrichment of ore, the mercury-antimony concentrate with the contents of 51.5% Sb, 3.55% Hg and 6 g/t Au was received. Vacuum distillation of concentrate allocation in serial sublimates mercury and antimony was designed. The big amount gold passed to calcine after vacuum distillation. The gold in calcine roastin uo to 900 ℃was 30.6 e/t.

成人低角型安氏II类1分类与安氏II类2分类错合畸形矫治前唇颏形态差异性研究

通过对比成人低角型安氏II类1分类和安氏II类2分类错合畸形患者的面部形态特点及唇颏形态,为临床制定矫治方案和临床治疗工作提供思路。方法 分别选取低角型安氏I类1分类和安氏II类2分类错合畸形患者40例,男女比例为1:1,年龄:18-25岁.通过将侧貌X线片和面部90°面相重合,对测量面部软组织21项指标进行测量。结果 上唇突点—E线距离,下唇突点—E线距离,上唇倾角,下唇倾角,上唇厚度,上下唇面积对比均有统计学意义(P<0.05)。结论 低角型安氏II类1分类与安氏II类2分类相比,上下唇均呈前突状,上唇面积,安氏II类1分类较安氏II类2分类小,下唇面积,安氏II类1分类较安氏II类2分类大。

Downregulation of Serum PTEN Expression in Mercury-Exposed Population and PI3K/AKT Pathway-Induced Inflammation

Objective This study investigated the impact of occupational mercury(Hg) exposure on human gene transcription and expression, and its potential biological mechanisms.Methods Differentially expressed genes related to Hg exposure were identified and validated using gene expression microarray analysis and extended validation. Hg-exposed cell models and PTEN lowexpression models were established in vitro using 293T cells. PTEN gene expression was assessed using qRT-PCR, and Western blotting was used to measure PTEN, AKT, and PI3K protein levels. IL-6 expression was determined by ELISA.Results Combined findings from gene expression microarray analysis, bioinformatics, and population expansion validation indicated significant downregulation of the PTEN gene in the high-concentration Hg exposure group. In the Hg-exposed cell model(25 and 10 μmol/L), a significant decrease in PTEN expression was observed, accompanied by a significant increase in PI3K, AKT, and IL-6 expression.Similarly, a low-expression cell model demonstrated that PTEN gene knockdown led to a significant decrease in PTEN protein expression and a substantial increase in PI3K, AKT, and IL-6 levels.Conclusion This is the first study to report that Hg exposure downregulates the PTEN gene, activates the PI3K/AKT regulatory pathway, and increases the expression of inflammatory factors, ultimately resulting in kidney inflammation.

青霜露暴露治疗II度烧烫伤临床观察

目的:观察青霜露暴露治疗烧烫伤的临床效果。方法:门诊II度烧烫伤患者100例,其中浅II度67例,深II度33例。以暴露疗法在烧烫伤创面青霜露局部用药,每日1~3次,直至愈合。结果:67例浅II度烧烫伤创面经青霜露治疗全部愈合,愈合时间最短6天,最长30天;33例深II度烧烫伤创面经青霜露治疗30天痊愈31例,显效2例,愈合时间最短14天。所有创面愈合良好,局部疼痛消失和无明显增生性瘢痕。结论:青霜露治疗烧烫伤促进创面愈合,疗效确切;本方法简单方便,值得临床推广应用。

Hybrid treatment of varied orthodontic appliances for a patient with skeletal class II and temporomandibular joint disorders:A case report and review of literature

BACKGROUND The relation between orthodontic treatment and temporomandibular disorders(TMDs)is under debate;the management of TMD during orthodontic treatment has always been a challenge.If TMD symptoms occur during orthodontic treatment,an immediate pause of orthodontic adjustments is recommended;the treatment can resume when the symptoms are managed and stabilized.CASE SUMMARY This case report presents a patient(26-year-old,female)with angle class I,skeletal class II and TMDs.The treatment was a hybrid of clear aligners,fixed appliances and temporary anchorage devices(TADs).After 3 mo resting and treatment on her TMD,the patient’s TMD symptom alleviated,but her anterior occlusion displayed deep overbite.Therefore,the fixed appliances with TAD were used to correct the anterior deep-bite and level maxillary and mandibular deep curves.After the levelling,the patient showed dual bite with centric relation and maximum intercuspation discrepancy on her occlusion.After careful examination of temporomandibular joints(TMJ)position,the stable bite splint and Invisible Mandibular Advancement appliance were used to reconstruct her occlusion.Eventually,the improved facial appearance and relatively stable occlusion were achieved.The 1-year follow-up records showed there was no obvious change in TMJ morphology,and her occlusion was stable.CONCLUSION TMD screening and monitoring is of great clinical importance in the TMD susceptible patients.Hybrid treatment with clear aligners and fixed appliances and TADs is an effective treatment modality for the complex cases.

血管活性药物评分联合APACHE II评分对血流感染后脓毒性休克患者预后的预测价值

目的:探讨血管活性药物评分(vasoactive inotropic score, VIS)联合急性生理与慢性健康评分(APACHE II)对血流感染后脓毒性休克患者结局的预测价值,为临床提供参考。方法:采用回顾性研究方法,通过收集2020年1月至2022年12月安徽医科大学附属合肥医院重症医学科收治的185例脓毒性休克患者的临床资料,根据28 d转归情况,分为存活组95例和死亡组90例,记录所有患者性别、年龄、相关血液检测指标、SOFA评分、24小时内急性生理与慢性健康评分(APACHE II)及24小时内最大血管活性药物评分(VISmax24)等信息,采用单因素分析筛选脓毒性休克患者的相关危险因素,多因素logistic回归分析影响脓毒性休克患者预后的独立危险因素,采用受试者工作特征(receiver op-erating characteristic, ROC)曲线下面积(area under curve, AUC)分析各指标的诊断价值。结果:单因素分析结果表明:死亡组的APACHE II评分(24.30 ± 5.157)、VISmax24 [100.0 (53.5, 121.0)]明显高于存活组(P max24联合APACHE II评分(AUC = 0.848, 95% CI: 0.792~0.905),较单一的APACHE II评分(AUC = 0.729, 95% CI: 0.655~0.802)、VISmax24 (AUC = 0.783, 95% CI: 0.709~0.856)、乳酸(AUC = 0.665, 95% CI: 0.587~0.743)、SOFA (AUC = 0.680, 95% CI: 0.602~0.758)预测价值更高。结论:VISmax24联合APACHE II评分能更有效预测血流感染后脓毒性休克患者的预后。