With the support by the National Natural Science Foundation of China,the research team led by Prof.Wang Di(王迪)at the Immuno metabolism Lab,Institute of Immunology,Zhejiang University School of Medicine,uncovered the...With the support by the National Natural Science Foundation of China,the research team led by Prof.Wang Di(王迪)at the Immuno metabolism Lab,Institute of Immunology,Zhejiang University School of Medicine,uncovered the mystery of Bile Acids control inflammation and metabolic disorder,which was published in Immunity(2016,45:802—816)).展开更多
Human growth hormone(GH)is a single-chain polypeptide of 191 amino acids that is involved in the regulation of various physiological processes,such as growth and metabolism.GH is synthesized and secreted by somatotr...Human growth hormone(GH)is a single-chain polypeptide of 191 amino acids that is involved in the regulation of various physiological processes,such as growth and metabolism.GH is synthesized and secreted by somatotropic cells in the anterior pituitary gland.Its secretion is primarily regulated by a balance between growth hormone-releasing hormone and growth hormone-inhibiting hormone,展开更多
The metabolite transport inhibition of tumor cells holds promise to achieve anti-tumor efficacy.Herein,we presented an innovative strategy to hinder the delivery of metabolites through the in-situ besieging tumor cell...The metabolite transport inhibition of tumor cells holds promise to achieve anti-tumor efficacy.Herein,we presented an innovative strategy to hinder the delivery of metabolites through the in-situ besieging tumor cells with polyphenolic polymers that strongly adhere to the cytomembrane of tumor cells.Simultaneously,these polymers underwent self-crosslinking under the induction of tumor oxidative stress microenvironment to form an adhesive coating on the surface of the tumor cells.This polyphenol coating effectively obstructed glucose uptake,reducing metabolic products such as lactic acid,glutathione,and adenosine triphosphate,while also causing reactive oxygen species to accumulate in the tumor cells.The investigation of various tumor models,including 2D cells,3D multicellular tumor spheroids,and xenograft tumors,demonstrated that the polyphenolic polymers effectively inhibited the growth of tumor cells by blocking key metabolite transport processes.Moreover,this highly adhesive coating could bind tumor cells to suppress their metastasis and invasion.This work identified polyphenolic polymers as a promising anticancer candidate with a mechanism by impeding the mass transport of tumor cells.展开更多
Single-atom nanozymes(SAZs)with peroxidase(POD)-like activity have good nanocatalytic tumor therapy(NCT)capabilities.However,insufficient hydrogen peroxide(H2O2)and hydrogen ions in the cells limit their therapeutic e...Single-atom nanozymes(SAZs)with peroxidase(POD)-like activity have good nanocatalytic tumor therapy(NCT)capabilities.However,insufficient hydrogen peroxide(H2O2)and hydrogen ions in the cells limit their therapeutic effects.Herein,to overcome these limitations,a biomimetic single-atom nanozyme system was developed for self-enhanced NCT.We used a previously described approach to produce platelet membrane vesicles.Using a high-temperature carbonization approach,copper SAZs with excellent POD-like activity were successfully synthesized.Finally,through physical extrusion,a proton pump inhibitor(PPI;pantoprazole sodium)and the SAZs were combined with platelet membrane vesicles to create PPS.Both in vivo and in vitro,PPS displayed good tumor-targeting and accumulation abilities.PPIs were able to simultaneously regulate the hydrogen ion,glutathione(GSH),and H2O2 content in tumor cells,significantly improve the catalytic ability of SAZs,and achieve self-enhanced NCT.Our in vivo studies showed that PPS had a tumor suppression rate of>90%.PPS also limited the synthesis of GSH in cells at the source;thus,glutamine metabolism therapy and NCT were integrated into an innovative method,which provides a novel strategy for multimodal tumor therapy.展开更多
文摘With the support by the National Natural Science Foundation of China,the research team led by Prof.Wang Di(王迪)at the Immuno metabolism Lab,Institute of Immunology,Zhejiang University School of Medicine,uncovered the mystery of Bile Acids control inflammation and metabolic disorder,which was published in Immunity(2016,45:802—816)).
基金supported by grants from the Korea Health 21 R&D Project(HI14C2348)the National Research Foundation of Korea(NRF2014R1A2A1A11051520)
文摘Human growth hormone(GH)is a single-chain polypeptide of 191 amino acids that is involved in the regulation of various physiological processes,such as growth and metabolism.GH is synthesized and secreted by somatotropic cells in the anterior pituitary gland.Its secretion is primarily regulated by a balance between growth hormone-releasing hormone and growth hormone-inhibiting hormone,
基金supported by the National Natural Science Foundation of China(52273302 and 52073230)the Ningbo Natural Science Foundation(2021J050)+1 种基金the Shaanxi Provincial Science Fund for Distinguished Young Scholars(2023-JC-JQ-32)the Fundamental Research Funds for the Central Universities,China。
文摘The metabolite transport inhibition of tumor cells holds promise to achieve anti-tumor efficacy.Herein,we presented an innovative strategy to hinder the delivery of metabolites through the in-situ besieging tumor cells with polyphenolic polymers that strongly adhere to the cytomembrane of tumor cells.Simultaneously,these polymers underwent self-crosslinking under the induction of tumor oxidative stress microenvironment to form an adhesive coating on the surface of the tumor cells.This polyphenol coating effectively obstructed glucose uptake,reducing metabolic products such as lactic acid,glutathione,and adenosine triphosphate,while also causing reactive oxygen species to accumulate in the tumor cells.The investigation of various tumor models,including 2D cells,3D multicellular tumor spheroids,and xenograft tumors,demonstrated that the polyphenolic polymers effectively inhibited the growth of tumor cells by blocking key metabolite transport processes.Moreover,this highly adhesive coating could bind tumor cells to suppress their metastasis and invasion.This work identified polyphenolic polymers as a promising anticancer candidate with a mechanism by impeding the mass transport of tumor cells.
基金the Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Cancer(No.2020B121201004)the Guangdong Provincial Major Talents Project(No.2019JC05Y361)+3 种基金the Outstanding Youths Development Scheme of Nanfang Hospital,Southern Medical University(No.2021J008)the Basic and Clinical Cooperative Research and Promotion Program of Anhui Medical University(No.2021xkjT028)the Open Fund of Key Laboratory of Antiinflammatory and Immune Medicine(No.KFJJ-2021-11)Grants for Scientific Research of BSKY from Anhui Medical University(No.1406012201).
文摘Single-atom nanozymes(SAZs)with peroxidase(POD)-like activity have good nanocatalytic tumor therapy(NCT)capabilities.However,insufficient hydrogen peroxide(H2O2)and hydrogen ions in the cells limit their therapeutic effects.Herein,to overcome these limitations,a biomimetic single-atom nanozyme system was developed for self-enhanced NCT.We used a previously described approach to produce platelet membrane vesicles.Using a high-temperature carbonization approach,copper SAZs with excellent POD-like activity were successfully synthesized.Finally,through physical extrusion,a proton pump inhibitor(PPI;pantoprazole sodium)and the SAZs were combined with platelet membrane vesicles to create PPS.Both in vivo and in vitro,PPS displayed good tumor-targeting and accumulation abilities.PPIs were able to simultaneously regulate the hydrogen ion,glutathione(GSH),and H2O2 content in tumor cells,significantly improve the catalytic ability of SAZs,and achieve self-enhanced NCT.Our in vivo studies showed that PPS had a tumor suppression rate of>90%.PPS also limited the synthesis of GSH in cells at the source;thus,glutamine metabolism therapy and NCT were integrated into an innovative method,which provides a novel strategy for multimodal tumor therapy.