The fermentation with mold metarrhizium anisopliae has been carried out to determine the relationship between mycelial morphology and growth.Like the biomass concentration,the mycelial morphology seems to be coupled t...The fermentation with mold metarrhizium anisopliae has been carried out to determine the relationship between mycelial morphology and growth.Like the biomass concentration,the mycelial morphology seems to be coupled to growth phases:the fractal dimension,a feature calculated from the morphological images,increases during the late lag phase and the early exponential phase,and culminates at a value near 2.4 at half of the exponential phase.This can be attributed to the marked change of rough and smooth pellet proportions during growth and the change can be well expressed by the fractal analysis of mycelial morphology.The mycelial morphology is also strongly related to the biotransformation activity:a mycelial sample was withdrawn from the culture to use for fractal analysis before the steroid substrate (16α,17α epoxy 4 pregnene 3,20 dione) was added;the higher fractal dimension corresponds to the higher conversion rate of steroid biotransformation.And the maximum of fractal dimension appeared prior to the maximum of biomass concentration.It is therefore possible to utilize the fractal dimension curve to supervise the fermentation process more timely and availably than to use the conventional biomass curve to do.展开更多
MetarhizosidesA-G(1-7),seven new polysubstituted phenyl glucosides,were isolated from the extracts of solid rice medium of a marine-derived fungus Metarrhizium anisopliae.Compounds 1-7 all contain a polysubstituted ph...MetarhizosidesA-G(1-7),seven new polysubstituted phenyl glucosides,were isolated from the extracts of solid rice medium of a marine-derived fungus Metarrhizium anisopliae.Compounds 1-7 all contain a polysubstituted phenyl group and the sugar unit is identified as 4'-O-methyl-β-D-glucopyranose.Their structures were elucidated by NMR spectroscopy and chemical method.These compounds were evaluated for anti-inflammatory activity by using LPS-stimulated murine macrophage RAW 264.7 cells and the cytotoxicities against four human cancer cell lines.展开更多
strains of Metarrhizium anisopliae and Metarrhizium guizhouense were cultivated in submerged culture with magnetized water, and the biomagnetic effects of magnetized water on submerged sporulation of Metarrhizium anis...strains of Metarrhizium anisopliae and Metarrhizium guizhouense were cultivated in submerged culture with magnetized water, and the biomagnetic effects of magnetized water on submerged sporulation of Metarrhizium anisopliae and Metarrhizium guizhouense were preliminarily studied. The results indicated that the magnetized water affected the production of submerged conidia. The output of submerged conidia were increased significantly in proper magnetized water medium. In addition, the infectivity of submerged conidia of M337 strain was tested in laboratory. The test showed that there were no significant differences in the toxicity to the 3rd～4th instar larvae of Dendrolimus punctatus between Submerged conidia harvested from medium of magnetized water and those from medium of non\|magnetized water. It is hoped that the results may be used as the basis for the study on large scale of submerged conidia of Metarrhizium anisopliae and Metarrhizium guizhouense cultivated by magnetized water.展开更多
基金National Natural Science Foundation of China forYoung Researcher !(No.2 960 60 0 8) Tianjin Natural ScienceFoundation!
文摘The fermentation with mold metarrhizium anisopliae has been carried out to determine the relationship between mycelial morphology and growth.Like the biomass concentration,the mycelial morphology seems to be coupled to growth phases:the fractal dimension,a feature calculated from the morphological images,increases during the late lag phase and the early exponential phase,and culminates at a value near 2.4 at half of the exponential phase.This can be attributed to the marked change of rough and smooth pellet proportions during growth and the change can be well expressed by the fractal analysis of mycelial morphology.The mycelial morphology is also strongly related to the biotransformation activity:a mycelial sample was withdrawn from the culture to use for fractal analysis before the steroid substrate (16α,17α epoxy 4 pregnene 3,20 dione) was added;the higher fractal dimension corresponds to the higher conversion rate of steroid biotransformation.And the maximum of fractal dimension appeared prior to the maximum of biomass concentration.It is therefore possible to utilize the fractal dimension curve to supervise the fermentation process more timely and availably than to use the conventional biomass curve to do.
基金This work was financially supported by the Program for Changjiang Scholars of Ministry of Education of the People's Republic of China(No.T2016088)the National Natural Science Foundation for Distinguished Young Scholars(No.81725021)+2 种基金the National Natural Science Foundation of China(No.31900288)the Program from the(hina Postdoctoral Science Foundation(No.2019M652660)the Integrated Innovative Team for Major Human Diseases Program of Tongji Medical College(HUST,China).
文摘MetarhizosidesA-G(1-7),seven new polysubstituted phenyl glucosides,were isolated from the extracts of solid rice medium of a marine-derived fungus Metarrhizium anisopliae.Compounds 1-7 all contain a polysubstituted phenyl group and the sugar unit is identified as 4'-O-methyl-β-D-glucopyranose.Their structures were elucidated by NMR spectroscopy and chemical method.These compounds were evaluated for anti-inflammatory activity by using LPS-stimulated murine macrophage RAW 264.7 cells and the cytotoxicities against four human cancer cell lines.
文摘本文在国内首次报道了分离自水稻二化螟（Chilo Suppressalis Walk）的一株黄绿绿僵菌（Metarrhizium flaviride Gams et Rozs）,此菌的主要特征是,菌落灰黄绿色,瓶梗杵状和有较宽的分生孢子（7.2～9.3×3～4μm）,这些特征与Gams et Rozs（1973）的原始描述基本一致。
文摘strains of Metarrhizium anisopliae and Metarrhizium guizhouense were cultivated in submerged culture with magnetized water, and the biomagnetic effects of magnetized water on submerged sporulation of Metarrhizium anisopliae and Metarrhizium guizhouense were preliminarily studied. The results indicated that the magnetized water affected the production of submerged conidia. The output of submerged conidia were increased significantly in proper magnetized water medium. In addition, the infectivity of submerged conidia of M337 strain was tested in laboratory. The test showed that there were no significant differences in the toxicity to the 3rd～4th instar larvae of Dendrolimus punctatus between Submerged conidia harvested from medium of magnetized water and those from medium of non\|magnetized water. It is hoped that the results may be used as the basis for the study on large scale of submerged conidia of Metarrhizium anisopliae and Metarrhizium guizhouense cultivated by magnetized water.
