METH1在酵母双杂交系统中的表达及对报告基因激活作用的检测

目的通过观察血管生成抑制因子METH1的cDNA片段在酵母双杂交中的表达及检测其对报告基因有无激活作用,为进一步明确METH1抑制增生性瘢痕的分子机制奠定基础。方法采用酵母双杂交Gal4系统3,经PCR扩增子METH1的cDNA片段,分别克隆入pUC19质粒,经测序正确后,再分别亚克隆入酵母双杂交诱饵载体pGBKT7中。将重组质粒导入酵母菌AH109,检测其表达产物在酵母细胞中对报告基因的激活作用。结果成功获得METH1的cDNA片段,该片段所表达的蛋白对酵母菌AH109无毒性,且对报告基因无激活作用。结论血管生成抑制因子METH1蛋白活性区在酵母双杂交系统中的表达产物,可作为诱饵蛋白进行相互作用蛋白的筛选研究。

METH1基因截短体的构建及其对HUVEC细胞增殖的影响

目的筛选血管生成抑制因子METH1功能编码区基因片段,为进一步研究METH1抑制增生性瘢痕的分子机制奠定基础。方法设计METH1三种基因截短体,分别构建相应的重组pcDNA3.1真核表达载体,然后将其稳定转染入HUVEC细胞,用RT-PCR、Western-Blot方法检测其在细胞中的表达,最后,通过MTT及FCM法验证其对HUVEC细胞增殖的影响。结果获得了能稳定表达的三种截短体,其中METH1-T2同METH1一样具有明显抑制HUVEC细胞增殖的作用,而METH1-T1、METH1-T3抑制细胞增殖的效果不明显。结论所获METH1-T2为功能活性区片段,可以替代METH1全长进行抑制增生性瘢痕的分子机制研究。

SQUARE WAVE COHERENT METHOD FOR EXTRACTING IP EFFECT

ＳＱＵＡＲＥＷＡＶＥＣＯＨＥＲＥＮＴＭＥＴＨＯＤＦＯＲＥＸＴＲＡＣＴＩＮＧＩＰＥＦＦＥＣＴ￥Ｈｅ，Ｊｉｓｈａｎ；Ｗａｎｇ，Ｓｈａｏｗｕ；ＴａｎｇＪｉｎｇｔｉａｎ（ＣｅｎｔｒａｌＳｏｕｔｈＵｎｉｖｅｒｃｉｔｙｏｆＴｅｃｈｎｏｌｏｇｙ，Ｃｈａｎｇｓｈａ，...

Novel boundary element method for resolving plate bending problems

This paper discusses the application of the boundary contour method fo r resolving plate bending problems. The exploitation of the integrand divergence free property of the plate bending boundary integral equation based on the Kirc hhoff hypothesis and a very useful application of Stokes' Theorem are presented to convert surface integrals on boundary elements to the computation of bending potential functions on the discretized boundary points,even for curved surface elements of arbitrary shape. Singularity and treatment of the discontinued corne r point are not needed at all. The evaluation of the physics variant at internal points is also shown in this article. Numerical results are presented for some plate bending problems and compared against analytical and previous solutions.

Accuracy of early detection of colorectal tumours by stool methylation markers:A meta-analysis

AIM: To evaluate the accuracy of methylation of genes in stool samples for diagnosing colorectal tumours.

Application of RP-HPLC method in dissolution testing and statistical evaluation by NASSAM for simultaneous estimation of tertiary combined dosages forms

A dissolution method with robust high performance liquid chromatographic （HPLC） analysis for im- mediate release tablet formulation was developed and validated to meet the requirement as per Inter- national Conference on Harmonization （ICH） and United States Food and Drug Administration （USFDA） guidelines. The method involved the use of Agilent ZORBAX Eclipse XDB C18 column, and temperature was maintained at 30 ℃. After optimization, the mobile phase was selected as phosphate buffer （KH2PO4, 30 mM） ： ACN （60：40, v/v） with pH 3.0, and retention time Rt was found as 3.24, 4.16, and 2.55 min for paracetamol （PCM）, chlorpheniramine maleate （CPM） and phenylephrine hydrochloride （PH） respec- tively at 265 nm and at a flow rate of 1 mL/min. The relative standard deviation （%RSD） for 6 replicate measurements was found to be less than 2%. Furthermore net analyte signal standard addition method （NASSAM） with spectrophotometer was performed for standard and liquid oral suspension. On the basis of selectivity, sensitivity and accuracy analysis, it was confirmed that this novel method could be useful for simultaneous estimation of the given drug combinations. Two-way analysis of variance （ANOVA） was applied for evaluating the statistical difference between the assay results obtained via both NASSAM and RP-HPLC methods and ultimately no significant difference was found between both the methods. All the methods and results were acceptable and confirmed that the method was suitable for intended use.

POLYMERIZATION OF(METH)ACRYLATES WITH ALUMINUM-BASED INITIATORS

A simple and effective way to prepare poly（acrylate）s, such as poly（methacrylate）, poly（butyl acrylate） and poly（butyl methacrylate）, has been achieved by using the single component aluminum-based compounds, such as modified methylaluminoxane （MMAO）, triisobutylaluminium （TIBA） and triethylaluminium （TEA） as initiators. Effective initiations and high molecular weight polymers with unimodal molecular weight distributions could be easily obtained by varying the reaction parameters of systems under mild conditions. Although these aluminum compounds were inefficient initiators for methyl methacrylate （MMA） polymerization, they exhibited remarkable catalytic activity for butyl methacrylate （BMA） polymerization, affording high molecular weight poly（BMA）s.

Helix-Sense-Selective Polymerization of N,N-Diphenyl (Meth)acrylamide by Anionic Catalysts

In this paper, the helix-sense-selective polymerization of N,N-diphenyl acrylamide (DPAA) and N,N-diphenyl methacrylamide(DPMAA) were studied with living helix prepolymer as anionic initiator, and the chiral optical properties of the obtained polymers were investigated too. It was shown that optically active polymers of DPAA and DPMAA could be obtained under the experimental condition, and exhibited the same screw sense as that of the prepolymer.

Exploration of Teaching Methods in English Reading Lessons

With the increasing of globalization on social life and economic activities,English has already become one of the basic and essential accomplishments for citizens all over the world.English education,in five-year higher vocational education,not only need adapt to the requirements of social and economic development,giving service in order to cultivate high-quality and high-skilled talents,but also should consider students' mental and emotional attitude as well as other factors,thereby meeting their stainable needs in employment and life after graduated.Therefore,the English education of five-year higher vocational education possesses multiply social and cultural significance.Though the analysis and understanding of the course objectives,ideas,contents and implementation suggestions brought up by The English Curriculum Standards of the Five-year Higher Vocational Education in Jiangsu Province,this paper explores the educational methods of higher vocational English reading lessons.

METH-108H型甲烷化催化剂还原探讨

介绍了赤天化股份公司对METH-108H型甲烷化催化剂还原的情况。还原在高空速,高一氧化碳浓度下进行,还原温度高,时间短,效果好。要保证METH-108H型甲烷化催化剂得到深度还原,利用高变气控制一氧化碳浓度是关键。

Theoretical Studies on the Mechanism of Mannich Reaction Involving Iminium Salt as Potential Mannich Reagent(4)——Use 2-Methylpropaldehyde as Pseudo-acid Component

以一个真实的Ｍａｎｎｉｃｈ试剂（ＣＨ３）２Ｎ＋ＣＨ２·Ｃｌ－作为研究对象．根据ＡＭ１方法的计算结果，胺、醛及伪酸在中性和酸性介质中的经典Ｍａｎｎｉｃｈ反应的关键步骤的活化能分别为３９０．０和３２１．８ｋＪ／ｍｏｌ．而由新Ｍａｎｎｉｃｈ试剂参与的Ｍａｎｎｉｃｈ反应的关键步骤活化能则为２７８．６ｋＪ／ｍｏｌ．因此新Ｍａｎｎｉｃｈ试剂可以提高反应速率，还分析了新Ｍａｎｎｉｃｈ试剂能降低反应活化能的原因．此外还讨论了另一种可大大降低活化能的新Ｍａｎｎｉｃｈ反应机理本文是研究Ｍａｎｎｉｃｈ反应机理的系列文章之一．

Reconstruct the form of unilateral cleft lip ala nasi and upper lip with improved Tennsion method to repair the function of orbicular muscle of mouth

Objective Improving the traditional Tennsion method to explore more reasonable re pair method for unilateral cleft lip.Methods37cases were operated to reconstruct the ala nasi and upper lip philtrum r idge form with rotatory alae nasi triangle flap plus improved Tennsion method,and compared the operational results with that of traditional Tennsion method.Results The improved Tennison method acquir ed a more beautiful nose and upper lip,it rehabilitated the ala nasi and restored t he physiological function of the orb iculars muscle.Conclusion Improved Tennsion method wins an essentially satisfactory operational result of unilateral cleft lip.

Solution and order number methods for multiple objective decision maki

Ｓｏｌｕｔｉｏｎａｎｄｏｒｄｅｒｎｕｍｂｅｒｍｅｔｈｏｄｓｆｏｒｍｕｌｔｉｐｌｅｏｂ－ｊｅｃｔｉｖｅｄｅｃｉｓｉｏｎｍａｋｉｎｇｗｉｔｈｉｎｃｏｍｐｌｅｔｅｋｎｏｗｌｅｄｇｅＪｉａｎｇＹｉｃｈｕ（ＤｅｐａｒｔｍｅｎｔｏｆＭａｔｈｅｍａｔｉｃｓ，Ｓｏｕ...

A SIMPLE PRECONDITIONED DOMAIN DECOMPOSITION METHOD FOR ELECTROMAGNETIC SCATTERING PROBLEMS

We present a domain decomposition method （DDM） devoted to the iterative solution of time-harmonic electromagnetic scattering problems, involving large and resonant cavities. This DDM uses the electric field integral equation （EFIE） for the solution of Maxwell problems in both interior and exterior subdomains, and we propose a simple preconditioner for the global method, based on the single layer operator restricted to the fictitious interface between the two subdomains.

Hi-Meth:特定位点DNA甲基化高通量检测平台

胞嘧啶甲基化是DNA表观遗传修饰的主要类型之一,在维持正常细胞功能和调控基因表达中具有重要作用。重亚硫酸盐测序法(bisulfite sequencing PCR,BSP)是特异性位点DNA甲基化检测的通用方法,能明确目的片段中每一个CpG位点的甲基化状态,但此方法需要大量的单克隆测序,操作过程较繁琐、成本昂贵。因此,开发准确、高效、便捷的DNA甲基化检测技术对提升表观遗传研究效率具有重要意义。基于本课题组开发的高通量突变类型检测平台Hi-TOM(high-throughput tracking of mutations),我们进一步建立了特定位点DNA甲基化高通量检测平台Hi-Meth(high-throughput detection of DNA methylation)。DNA样品通过重亚硫酸盐处理之后,仅需一轮PCR扩增即可通过Hi-Meth平台获得特定位点DNA甲基化分析结果。利用Hi-Meth平台,对水稻不同基因启动子区域进行了DNA甲基化检测分析,并与基于BSP方法获得的结果进行了比较。结果表明,Hi-Meth策略与BSP策略检测结果基本一致。而且通过Hi-Meth平台可以更准确、便捷地获得特异性位点DNA甲基化分析结果。综上所述,Hi-Meth为特定DNA区域提供了重要的甲基化检测平台,对表观遗传研究具有重要意义。

Path Planning in Complex 3D Environments Using a Probabilistic Roadmap Method

This paper presents a 3D path planning algorithm for an unmanned aerial vehicle （UAV） in complex environments. In this algorithm, the environments are divided into voxels by octree algorithm. In order to satisfy the safety requirement of the UAV, free space is represented by free voxels, which have enough space margin for the UAV to pass through. A bounding box array is created in the whole 3D space to evaluate the free voxel connectivity. The probabilistic roadmap method （PRM） is improved by random sampling in the bounding box array to ensure a more efficient distribution of roadmap nodes in 3D space. According to the connectivity evaluation, the roadmap is used to plan a feasible path by using A＊ algorithm. Experimental results indicate that the proposed algorithm is valid in complex 3D environments.

Intervention Effect of Repetitive TMS on Behavioral Adjustment After Error Commission in Long-Term Methamphetamine Addicts： Evidence From a Two-Choice Oddball Task

Behavioral adjustment plays an important role in the treatment and relapse of drug addiction. Nonetheless,few studies have examined behavioral adjustment and its plasticity following error commission in methamphetamine（METH） dependence, which is detrimental to human health. Thus, we investigated the behavioral adjustment performance following error commission in long-term METH addicts and how it varied with the application of repetitive transcranial magnetic stimulation（r TMS） of the left dorsolateral prefrontal cortex（DLPFC）. Twenty-nine male long-term METH addicts（for [ 3 years） were randomly assigned to high-frequency（10 Hz, n = 15） or sham（n = 14） r TMS of the left DLPFC during a two-choice oddball task. Twenty-six age-matched, healthy male adults participated in the two-choice oddball task pretest to establish normal performance for comparison. The results showed that 10 Hz r TMS over the left DLPFC significantly decreased the post-error slowing effect in response times of METH addicts. In addition, the 10 Hz r TMS intervention remarkably reduced the reaction times during post-error trials but not post-correct trials. While the 10 Hz r TMS group showed a more pronounced post-error slowing effect than the healthy participants during the pretest, the posterror slowing effect in the posttest of this sample was similar to that in the healthy participants. These results suggest that high-frequency r TMS over the left DLPFC is a useful protocol for the improvement of behavioral adjustment after error commission in long-term METH addicts.

A NOTE ON JACOBI SPECTRAL-COLLOCATION METHODS FOR WEAKLY SINGULAR VOLTERRA INTEGRAL EQUATIONS WITH SMOOTH SOLUTIONS

This work is concerned with spectrM Jacobi-collocation methods for Volterra integral equations of the second kind with a weakly singular of the form （t - s）-a When the underlying solutions are sufficiently smooth, the convergence analysis was carried out in [Chen ＆ Tang, J. Comput. Appl. Math., 233 （2009）, pp. 938-950]; due to technical reasons 1 In this work, we will improve the results to the the results are restricted to 0 〈 μ 〈 1/2. general case 0 〈 μ 〈 1 and demonstrate that the numericl errors decay exponentially in the infinity and weighted norms when the smooth solution is involved.

Synthesis and biological evaluation of a lipopeptide-based methamphetamine vaccine

We describe an application of carrier protein-free strategy in constructing a fully synthetic methamphetamine(METH)vaccine that contains three components:Toll-like receptor 2 ligand,Th2 epitope,and METH hapten.The immunological evaluation in mice revealed high titers of METH-specific antibodies induced by the construct and the activation of humoral immunity that would be beneficial for neutralization and clearance of the METH molecule.Behavio ral experiments indicated that the synthetic vaccine attenuated the acquisition of METH-induced conditioned place preference and inhibited the initiation and expression of METH-induced locomotor sensitization.These results demonstrate that the lipopeptide-based vaccine has invo ked an immune response and showed the potential of preventing the rewarding and psychoactive effects of METH.

RECURSIVE METH0D FOR ARMA MODEL ESTIMATION (Ⅱ)

In this paper a new recursive method for ARMA model estimation is given. Same as in [1], theorder's estimator is strongly consistent, and the parameter's estimators defer to CLT and LILunder a natural condition. Compared with the previous metheds suggested by Hannan & Kavalieris(1984), Wang Shouren & Chen Zhaoguo (1985) and Franke (1985), this methed has some advantages:the amount of calculat on work is smaller, the minimum-phase property of coeffcient estimators canbe guaranteed,the BAN estimators for MA or AR model can be obtained directly,and the simulationshows that this method is more accurate in estimating the order and parameters.