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Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China 被引量:16
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作者 WANG Wei LIU Feng +12 位作者 ZULQARNAIN Baloch ZHANG Cun Shan MA Ke PENG Zi Xin YAN Shao Fei HU Yu Jie GAN Xin DONG Yin Ping BAI Yao LI Feng Qin YAN Xiao Mei MA Ai Guo XU Jin 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2017年第8期570-580,共11页
Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes a... Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing (MLST), spa typing, multiple-locus variable number of tandem repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance (MDR). Greater diversity was found in food-associated MRSA (7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA (3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates (40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone (96.4%), and CC9-ST9-t437-MC621 (20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig- and food-associated isolates. Of note, some community- and hospital-associated MRSA strains (t030, t172, t1244, and t4549) were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China. 展开更多
关键词 methicillin-resistant staphylococcus aureus Antimicrobial susceptibility Genetic diversity PIG Retail food
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Detection and Characterization of Methicillin Resistant Staphylococcus aureus from Toilet and Classroom Door Handles in Selected Secondary Schools in Nairobi County 被引量:5
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作者 Caroline Mbogori Anne Muigai Samuel Kariuki 《Open Journal of Medical Microbiology》 2013年第4期248-252,共5页
Background: Staphylococcus aureus is found on all surfaces especially in public areas like hospitals and schools and on frequently touched areas like toilet and classroom door handles. Methicillin resistant Staphyloco... Background: Staphylococcus aureus is found on all surfaces especially in public areas like hospitals and schools and on frequently touched areas like toilet and classroom door handles. Methicillin resistant Staphylococcus aureus (MRSA) is a strain of Staphylococcus aureus which is resistant to methicillin. There are two types of MRSA: Community acquired methicillin resistant Staphylococcus aureus (CA-MRSA) and hospital acquired methicillin resistant Staphylococcus aureus (HA-MRSA). MRSA in the community presents a significant reservoir that could enter into healthcare facilities and spread among patients and also a risk for immune compromised persons in the community. Methodology: The study aimed at determining the prevalence of MRSA isolated from toilet and classroom door handles as a potential source of infection to the students and the workers in selected schools in Nairobi, Kenya. The study also compared the prevalence of MRSA between boarding and non-boarding girls, boys and mixed (both girls and boys in the same school) secondary schools. Twelve secondary schools in Nairobi County were randomly selected and 306 samples from both the toilet and classroom door handles were collected using sterile swabs and transported to the laboratory. Isolation of Staphylococcus aureus was done by the use of selective media Mannitol salt agar, antibiotic susceptibility of isolates was done by disk diffusion method, and molecular detection of mecA and PVL genes were done by polymerase chain reaction (PCR). Results: The prevalence of S. aureus was 20% and 15% were MRSA positive by both Antimicrobial Susceptibility Test and PCR detection. 20% showed the presence of PVL genes, 8% showed the presence of both genes and 56% of isolates with mecA gene had PVL genes. Conclusion: The presence of MRSA in this study emphasizes the need to formulate hygiene measures to prevent possible spread of MRSA and other transmissible pathogens to students and workers in the schools. 展开更多
关键词 methicillin resistant staphylococcus aureus Secondary SCHOOLS Antibiotic Patterns
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Potentiating activity of luteolin on membrane permeabilizing agent and ATPase inhibitor against methicillin-resistant Staphylococcus aureus 被引量:4
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作者 Dae-Ki Joung Young-Seob Lee +8 位作者 Sin-Hee Han Sang-Won Lee Seon-Woo Cha Su-Hyun Mun Ryong Kong Ok-Hwa Kang Ho-Jun Song Dong-Won Shin Dong-Yeul Kwon 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2016年第1期16-19,共4页
Objective:To investigate the mechanism of antibacterial activity of luteoiin(LUT) against methicillin-resistant Staphylococcus aureus(MRSA).Methods:The mechanism of anti-MRSA activity of LUT was analyzed by the viabil... Objective:To investigate the mechanism of antibacterial activity of luteoiin(LUT) against methicillin-resistant Staphylococcus aureus(MRSA).Methods:The mechanism of anti-MRSA activity of LUT was analyzed by the viability assay in membrane permeabilizing agent ATPase inhibitors,and peptidoglycan(PGN) derived from Staphylococcus aureus(S.aureus).Also,transmission electron microscopy was used to monitor survival characteristics and changes in S.aureus morphology.Results:Compared to the LUT alone,the optical density of suspensions treated with the combination of 125 μg/mL Tris and 230 μg/mL DCCD were reduced to 60%and 46%,respectively.PGN(15.6 μg/mL) gradually impeded the activity of LUT,and PGN(62.5 μg/mL) completely blocked the activity of LUT on S.aureus.Conclusions:Increased susceptibility to LUT with me Tris and DCCD combinations is evident in all tested MRSA isolates.The results indicate LUT synergy in increasing cytoplasmic membrane permeability and inhibiting ATPase.S.aureus PGN directly blocks the antibacterial activity of LUT,suggesting the direct binding of LUT with PGN.These findings may be validated for the development of antibacterial agent for low MRSA resistance. 展开更多
关键词 Iuteolin methicillin-resistant staphylococcus aureus MEMBRANE permeabilizing AGENT ATPASE INHIBITOR PEPTIDOGLYCAN
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Postoperation of preauricular fistula cellulitis caused by methicillin-resistant staphylococcus aureus infection 被引量:3
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作者 Haiyue Tian Cuiping Zhong 《Journal of Otology》 CSCD 2018年第3期111-113,共3页
A one-year-old baby girl with one-month history of recurrent pus fluid exuding from her left preauricular sinus orifice, who failed multiple courses of surgical drainage of the abscess and persistent debridement for t... A one-year-old baby girl with one-month history of recurrent pus fluid exuding from her left preauricular sinus orifice, who failed multiple courses of surgical drainage of the abscess and persistent debridement for the wound, presented with MRSA infection. The patient was treated with linezolid for three days. Her pain and paresthesia resolved, and C-reactive protein decreased to normal. 展开更多
关键词 Preauricularsinus Surgery OVERUSE ANTIBIOTICS methicillin-resistant staphylococcus aureus CELLULITIS
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Implantable cardioverter defibrillator lead-related methicillin resistant Staphylococcus aureus endocarditis:Importance of heightened awareness 被引量:1
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作者 Obiora F Anusionwu Cheri Smith Alan Cheng 《World Journal of Cardiology》 CAS 2012年第7期231-233,共3页
Methicillin resistant Staphylococcus aureus(MRSA) septicemia is associated with high morbidity and mortality especially in patients with immunosuppression,diabetes,renal disease and endocarditis.There has been an incr... Methicillin resistant Staphylococcus aureus(MRSA) septicemia is associated with high morbidity and mortality especially in patients with immunosuppression,diabetes,renal disease and endocarditis.There has been an increase in implantation of cardiac implantable electronic devices(CIED) with more cases of devicelead associated endocarditis been seen.A high index of suspicion is required to ensure patient outcomes are optimized.The excimer laser has been very efficient in helping to ensure successful lead extractions in patients with CIED infections.We present an unusual case report and literature review of MRSA septicemia from device-lead endocarditis and the importance of early recognition and prompt treatment. 展开更多
关键词 ENDOCARDITIS methicillin resistant staphylococcus aureus Sepsis PACEMAKERS Implantable CARDIOVERTER-DEFIBRILLATOR
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Antimicrobial activity and synergism of Sami-Hyanglyun-Hwan with ciprofloxacin against methicillin-resistant Staphylococcus aureus 被引量:2
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作者 Jang-Gi Choi Ji-Young Choi +5 位作者 Su-Hyun Mun Ok-Hwa Kang Preeti Bharaj Dong-Won Shin Myong-Soo Chong Dong-Yeul Kwon 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2015年第7期536-540,共5页
Objective: To investigate the antibacterial activity of SHHextracted with either water or ethanol against methicillin-resistant Staphylococcus aureus(MRSA) and combinatory antimicrobial effect with ciprofloxacin(CIP) ... Objective: To investigate the antibacterial activity of SHHextracted with either water or ethanol against methicillin-resistant Staphylococcus aureus(MRSA) and combinatory antimicrobial effect with ciprofloxacin(CIP) by time kill assay and checkerboard dilution test. Methods: The antibacterial activity determined by broth dilution method indicated that the antibacterial activity of Sami-Hyanglyun-Hwan(SHH) water extract(SHHW) and SHH ethanol extract(SHHE) ranged from 250 to 2000 μg/m L and 125 to 1000 μg/m L against MRSA, respectively. Results: In the checkerboard method, the combinations of SHHE with CIP had a partial synergistic or synergistic effect against MRSA. The time-kill curves showed that a combined SHHE and CIP treatment reduced the bacterial counts dramatically after 24 h. Conclusions: The present study demonstrates the therapeutic ability of SHHE against MRSA infections. 展开更多
关键词 Sami-Hyanglyun-Hwan methicillin-resistant staphylococcus aureus CIPROFLOXACIN SYNERGY
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Molecular typing of methicillin and vancomycin-resistant Staphylococcus aureus isolated from clinical specimens by double locus sequence typing(DLST)method 被引量:1
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作者 SOHEILA HASANIAN NOUR AMIRMOZAFARI +1 位作者 AHMAD FARAJZADEH SHEIKH IRAJ MEHREGAN 《BIOCELL》 SCIE 2020年第3期411-419,共9页
Methicillin-resistant Staphylococcus aureus(MRSA)strains are the essential cause of infections in communities and hospitals.The present study was conducted to determine the molecular typing of MRSA,isolated from hospi... Methicillin-resistant Staphylococcus aureus(MRSA)strains are the essential cause of infections in communities and hospitals.The present study was conducted to determine the molecular typing of MRSA,isolated from hospitalized patients,using the double-locus sequence typing(DLST).In total,280 S.aureus isolated from clinical specimens by phenotypic(catalase,coagulase,DNase,oxacillin,vancomycin screening agar and antibiotic disk diffusion),and molecular methods(PCR for determining the mecA,vanA and nuc genes).The DLST and sequencing was performed for MRSA containing mecA.Out of 280 specimens,confirmed as Staphylococcus aureus(S.aureus),123(43.9%)strains were MRSA.The highest resistance toward the erythromycin(15μg),followed by ciprofloxacin(5μg),clindamycin(2μg),tetracycline(30μg),gentamicin(10μg)and rifampicin(5μg),was 98.3%,97.5%,94.3%,90.2%,83.7%and 41.4%,respectively.Also,the least resistance(0%)was observed in each of teicoplanin(30μg),linzolide(30μg),and vancomycin(30μg).All(100%)of MRSA strains had the mecA,and none of them have had the vanA.The results of DLST showed that the most common sequence types were BPH 2003 and 0217.The DLST type 18-32 was a significant cluster of MRSA.By sequencing MRSA and comparing the dominant types via the DLST,it is possible to establish the etiology of the disease in a much shorter time,and prevent the complications of the disease.Therefore,the combination of partial sequences of clfB and spa can serve as useful genetic markers for MRSA typing.It concluded that the MRSA in our region was relatively high,but no vancomycin resistance was found.The majority of the MRSA DLST type was 18–32. 展开更多
关键词 Antibiotic resistance DLST mrsa staphylococcus aureus
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Antibacterial Activity of Exogenous Glutathione and Its Synergism on Antibiotics in Methicillin-Associated Multidrug Resistant Clinical Isolates of Staphylococcus aureus 被引量:1
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作者 Cleiford Senra Minerva A. Garcia Dong H. Kwon 《Advances in Microbiology》 CAS 2022年第12期635-648,共14页
Background: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most problematic human pathogens. Antibiotic treatment of MRSA often associated with resistance to multiple classes of antibiotics is extrem... Background: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most problematic human pathogens. Antibiotic treatment of MRSA often associated with resistance to multiple classes of antibiotics is extremely challenging and urgently demands action to treat MRSA. Glutathione (GSH) is a biogenic thiol-compound that maintains an optimal intracellular redox-potential required for various normal cellular processes. Antibacterial activity of exogenous GSH has been reported in some bacterial pathogens but is largely unknown in MRSA. Aim: This study aimed to understand antibacterial activity of GSH, its role in antibiotic susceptibility, and a potential antibacterial mechanism in clinical isolates of S. aureus. Materials and Methods: Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), checkerboard, time-killing, and bacterial killing assays were performed for 14 clinical isolates of S. aureus including 10 MRSA and two type strains (ATCC 700699 and 35556). Results: MIC and MBC levels for the clinical and type strains were 15 - 20 mM and 25 - 40 mM of GSH, respectively. Subinhibitory concentrations of GSH synergistically enhanced susceptibility of all tested-antibiotics, resulting in sensitizing all-tested S. aureus. Bacterial-killing produced by GSH-mediated acidity was significantly higher than that by hydrochloric acid-mediated acidity. Conclusion: Overall results concluded that GSH exhibited antibacterial activity on S. aureus regardless of antibiotic susceptibility and synergistically enhanced antibiotic susceptibility. Additionally, GSH-mediated acidity was one of the antibacterial mechanisms. These findings suggest that GSH may be a potential antimicrobial agent or adjuvant for the conventional anti-MRSA regimens. 展开更多
关键词 methicillin-resistant staphylococcus aureus (mrsa) Multidrug Resistance GLUTATHIONE Antibacterial Activity
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Synergistic Effect between Fucoidan and Antibiotics against Clinic Methicillin-Resistant <i>Staphylococcus aureus</i> 被引量:2
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作者 Sung-Mi Choi Eun-Jin Jang Jeong-Dan Cha 《Advances in Bioscience and Biotechnology》 2015年第4期275-285,共11页
Fucoidan is a sulfated polysaccharide that is primarily extracted from brown seaweeds which has been broadly studied in recent years due to its numerous biological properties, including anticoagulant, antithrombotic, ... Fucoidan is a sulfated polysaccharide that is primarily extracted from brown seaweeds which has been broadly studied in recent years due to its numerous biological properties, including anticoagulant, antithrombotic, antitumor, and antiviral activities. In this study, fucoidan was evaluated against clinic isolated methicillin-resistant Staphylococcus aureus (MRSA) 1 - 20, either alone or with antibiotics, via broth dilution method and checkerboard and time kill assay. Minimum inhibitory concentrations (MICs)/Minimum bactericidal concentrations (MBCs) values for the fucoidan against all the tested bacteria ranged between 64 - 512/256 - 2048 microg/mL, for ampicillin 32 - 1024/64 - 1024 microg/mL and for oxacillin 8 - 64/16 - 256 microg/mL respectively. Furthermore, the MIC and MBC were reduced to one half-eighth as a result of the combination of the fucoidan with antibiotics. 2 - 6 hours of treatment with 1/2 MIC of fucoidan with 1/2 MIC of antibiotics resulted from an increase of the rate of killing in units of CFU/mL to a greater degree than was observed with alone. These results suggest that fucoidan could be employed as a natural antibacterial agent against multi-drug bacteria. 展开更多
关键词 FUCOIDAN Antibacterial Activity methicillin-resistant staphylococcus aureus Synergistic Effect Minimum Inhibitory CONCENTRATIONS (MICs) Minimum BACTERICIDAL CONCENTRATIONS (MBCs)
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Treatment of methicillin-resistant Staphylococcus aureus infections:Importance of high vancomycin minumum inhibitory concentrations 被引量:1
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作者 Alejandra Morales-Cartagena Antonio Lalueza +2 位作者 Francisco López-Medrano Rafael San Juan José María Aguado 《World Journal of Clinical Infectious Diseases》 2015年第2期14-29,共16页
Staphylococcus aureus(SA) infections remain a major cause of morbidity and mortality despite the availability of numerous effective anti-staphylococcal antibiotics.This organism is responsible for both nosocomial and ... Staphylococcus aureus(SA) infections remain a major cause of morbidity and mortality despite the availability of numerous effective anti-staphylococcal antibiotics.This organism is responsible for both nosocomial and community-acquired infections ranging from relatively minor skin and soft tissue infections to life-threateningsystemic infections.The increasing incidence of methicillin-resistant strains has granted an increasing use of vancomycin causing a covert progressive increase of its minimum inhibitory concentration(MIC)(dubbed the MIC "creep").In this way,the emergence of vancomycinintermediate SA(VISA) strains and heteroresistantVISA has raised concern for the scarcity of alternative treatment options.Equally alarming,though fortunately less frequent,is the emergence of vancomycin-resistant SA.These strains show different mechanisms of resistance but have similar problems in terms of therapeutic approach.Ultimately,various debate issues have arisen regarding the emergence of SA strains with a minimum inhibitory concentration sitting on the superior limit of the sensitivity range(i.e.,MIC = 2 μg/mL).These strains have shown certain resilience to vancomycin and a different clinical behaviour regardless of vancomycin use,both in methicillin-resistant SA and in methicillin-sensitive SA.The aim of this text is to revise the clinical impact and consequences of the emergence of reduced vancomycin susceptibility SA strains,and the different optimal treatment options known. 展开更多
关键词 staphylococcus aureus Minimum INHIBITORY concentration methicillin-resistant staphylococcus aureus Vancomycin-intermediate staphylococcus aureus Heteroresistant-vancomycin-intermediate staphylococcus aureus VANCOMYCIN resistant staphylococcus aureus
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Combination of Acacetin with Antibiotics against Methicillin Resistant <i>Staphylococcus aureus</i>Isolated from Clinical Specimens 被引量:1
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作者 Jeong-Dan Cha Sung-Mi Choi Jeong Hye Park 《Advances in Bioscience and Biotechnology》 2014年第4期398-408,共11页
Methicillin-restitant Staphylococcus aureus (MRSA) is very dangerous bacteria and one of the most feared nosocomial germs. In this study, acacetin was evaluated against 20 clinical isolates of MRSA, either alone or in... Methicillin-restitant Staphylococcus aureus (MRSA) is very dangerous bacteria and one of the most feared nosocomial germs. In this study, acacetin was evaluated against 20 clinical isolates of MRSA, either alone or in combination with antibiotics. The acacetin exhibited a good activity against isolates MRSA with MICs/MBCs ranged between 10-80/20-160 μg/mL, for ampicillin 64-1024/128-2048 μg/mL, and for oxacillin 8-32/16-64 μg/mL. The combination of acacetin plus oxacillin or ampicillin was reduced by ≥4-fold against isolates MRSA tested, evidencing a synergistic effect as defined by a FICI of ≤0.5. Furthermore, a time-kill study evaluating the growth of the tested bacteria was completely attenuated after 2-5 h of treatment with the 1/2 MIC of acacetin, regardless of whether it was administered alone or with oxacillin (1/2 MIC) or ampicillin (1/2 MIC). In conclusion, acacetin exerted synergistic effects when administered with oxacillin or ampicillin and the antibacterial activity and resistant regulation of acacetin against clinical isolates of MRSA might be useful in controlling MRSA infections. 展开更多
关键词 ACACETIN methicillin-resistant staphylococcus aureus Minimum INHIBITORY CONCENTRATIONS Minimum Bactericidal CONCENTRATIONS Time-Kill Curves Fractional INHIBITORY Concentration
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The prevalence of methicillin-resistant Staphylococcus aureus colonization in emergency department fast track patients 被引量:1
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作者 Kelly Williamson April Bisaga +1 位作者 Katherine Paquette Elise Lovell 《World Journal of Emergency Medicine》 CAS 2013年第4期278-280,共3页
BACKGROUND:Over the past two decades,methicillin-resistant Staphylococcus aureus(MRSA) has evolved from a hospital-associated infection to a significant public health threat in the community,causing outbreaks of soft ... BACKGROUND:Over the past two decades,methicillin-resistant Staphylococcus aureus(MRSA) has evolved from a hospital-associated infection to a significant public health threat in the community,causing outbreaks of soft tissue infections in otherwise healthy individuals.The goal of this study was to determine the prevalence of nasal MRSA colonization in low acuity Emergency Department(ED) Fast Track patients in order to better characterize the epidemiology of this pathogen.METHODS:We conducted a cross-sectional study of a convenience sample of adult patients from our ED Fast Track.Nasal swabs were analyzed for MRSA using a polymerase chain reaction assay.Study participants completed a survey assessing traditional risk factors for CA-MRSA colonization.RESULTS:A total of 106 ED Fast Track patients were tested.Four(3.8%,95%CI 1.5%-9.3%)were MRSA positive.Three traditional CA-MRSA risk factors(personal history of abscess,family history of abscess,and participation in contact sports) were examined.In patients with a positive MRSA nasal swab,only a personal prior history of abscess retained significance(OR 33,95%C11.7-676,P=0.02).CONCLUSION:This study found a higher prevalence of nasal MRSA colonization in low acuity ED Fast Track patients compared with historical community surveillance studies.A personal history of prior abscess was a significant risk for CA-MRSA carriage. 展开更多
关键词 methicillin-resistant staphylococcus aureus Emergency department
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Intestinal carriage of methicillin resistant Staphylococcus aureus and extended-spectrum β-Lactamase-producing Enterobacteriacae in hospitalized and nonhospitalized patients and their clinical implications
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作者 Hanan Ahmed Habib Babay Ali Mohammed Somily 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2009年第3期41-45,共5页
Objective:To determine the clinical implication of and intestinal carriage with methicillin resistant Staphylococcus aureus(MRSA) and extended spectrumβ-lactamase(ESBL)-producing Enterobacteriacae.Methods: A total of... Objective:To determine the clinical implication of and intestinal carriage with methicillin resistant Staphylococcus aureus(MRSA) and extended spectrumβ-lactamase(ESBL)-producing Enterobacteriacae.Methods: A total of 180 stool specimens were screened for MRSA and ESBL-producing enterobacteria.Identification of ESBL- producing Enterobacteriacae was done by MicroScan Walk Away 96 system(Dade Behring Inc.,West Sacramento,CA 95691,USA ) and confirmation by double-disc synergy test.MRSA was identified by disc diffusion using 30μg cefoxitin disc and the MicroScan.Results:The rate of fecal MRSA carriage was 7.8% (14/180),35.7%(5 /14) were recovered from surgical wards.Three patients(21,4%) had MRSA recovered from other body sites,and 2(14.2%) had in addition ESBL -producing Escherichia coli(E.coli) and Klebsiella pneumoniae(K.pneumoniae) respectively.Four(28.5%) patients with MRSA fical carriage died. MRSA fecal carriage was recovered from both inpatients and outpatients.Four(2.2%) cases carried ESBL-producing Enterobacteriacae in feces.Three(75%) were from intensive care unit(ICU).One patient had both ESBL-producing E.coli and K.pneumoniae from stool as well as E.coli from tracheal aspirate.Two ICU patients with fecal ESBL died.Conclusion:Fecal screening for MRSA and ESBL of all patients at high risk admitted to different hospital wards and ICUs and implementing infection control measures were recommended. 展开更多
关键词 INTESTINAL CARRIAGE methicillin resistant staphylococcus aureus EXTENDED spectrumβ-lactamase Enterobacteriacae
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Identification of Panton-Valentine leukocidin virulence gene in methicillin-resistant Staphylococcus aureus isolated from clinical specimens of burn patients in Zare Hospitals of Sari, Iran
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作者 Atefeh Ranjkesh Zahra Salari +14 位作者 Mehrab Nejati Emad Behboudi Ali Ramezani Milad Zandi Mahmood Marzban Hadis Kiani Mina Bavi Mehdi Rabiei Roodsari Yousef Esvand Zibaee Samaneh Abbasi Maryam Mohammadi Elyasi Mehdi Parsanahad Shahram Jalilian SiminMohammadi Gorji Mina Owrang 《Journal of Acute Disease》 2020年第3期121-125,共5页
Objective:To identify the pathogenic gene of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn Hospital.Methods:A ... Objective:To identify the pathogenic gene of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn Hospital.Methods:A total of 104 samples of Staphylococcus were collected and 78 aureus samples were isolated from Zare Hospital patients from November 2016 to July 2017.All isolates were identified using a standard biochemical and laboratory methodology in accordance with CLSI principles,and disk agar diffusion antibiogram were performed to identify methicillin resistant colonies.Then the presence of the Panton-Valentine leukocidin gene was tested by PCR.Results:Of the methicillin-resistant Staphylococcus aureus samples,80%were negative for the Panton-Valentine leukocidin gene,and only 20%of the samples had Panton-Valentine leukocidin gene.Male and female patients showed no significant difference in the positivity rate of Panton-Valentine leukocidin gene(16.12%vs.33.33%)(P=0.25).Besides,there was no significant difference in bacterial resistance or susceptibility to antibiotics between samples with or without the Panton-Valentine leukocidin gene.Conclusions:In recent years,increased resistance has been a serious threat.The resistance or susceptibility of Staphylococcus aureus strains to different antibiotics is different in different geographical locations. 展开更多
关键词 methicillin Resistance Panton-Valentine leukocidin PCR staphylococcus aureus
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Incidence of Methicillin Resistant Staphylococcus aureus in Burn Patients Admitted to Burn Unit, Dhaka Medical
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作者 Md. Shariful Islam M. Firoz Ahmed Sabita Rezwana Rahman 《Advances in Microbiology》 2013年第6期498-503,共6页
Staphylococcus aureus infection remains an increasing problem for higher morbidity and mortality in burn patients. We sought to determine the frequency of methicillin-resistant S. aureus (MRSA) in burn wound patients ... Staphylococcus aureus infection remains an increasing problem for higher morbidity and mortality in burn patients. We sought to determine the frequency of methicillin-resistant S. aureus (MRSA) in burn wound patients and study their drug resistance genes. Samples were collected (August 2010 to October 2011) from burn unit of Dhaka Medical College Hospital (DMCH), Bangladesh. MRSA was identified by conventional culture based methods. S. aureus was confirmed in 44.44% burn wound samples and 22.5% of the isolates were oxacillin resistant. All the S. aureus isolates were resistant to commonly used drugs like amoxicillin, azactam, erythromycin, azithromycin etc. and were sensitive to imipenem. The isolates were devoid of plasmid and the gene mecA, femA and IS431 were detected in their chromosomal DNA. Computational analysis of mecA gene sequence showed homology to S. aureus “penicillin binding protein 2a” (PBP-2a). The higher association of MRSA is in burn unit of DMCH, Bangladesh is alarming and with inappropriate antibiotic use, and the situation gets even complicated to treat. Therefore, the detection system and control practices for MRSA in DMCH should be improved in the hospital settings. 展开更多
关键词 methicillin resistant staphylococcus aureus (mrsa) MULTIDRUG resistant (MDR) Minimum INHIBITORY Concentration (MIC)
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Co-Habitation of <i>Staphylococcus lugdunensis</i>with <i>Staphylococcus aureus</i>Resistant to Methicillin and Vancomycin in the Nasal Snares of Laboratory Rats
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作者 Lorina Ineta Badger-Emeka 《Advances in Microbiology》 2017年第1期47-55,共9页
The public health problem created by multidrug resistant bacteria in the 21st century continues to receive attention by researchers all over the world. As the production of new antibiotics is not commeasurable with th... The public health problem created by multidrug resistant bacteria in the 21st century continues to receive attention by researchers all over the world. As the production of new antibiotics is not commeasurable with the rate of evolvement of MDR bacteria, the news of a proposed new antibiotic “Lugdunin” is much awaited and a welcomed development. Lugdunin is produced by Staphylococcus lugdunensis and has the ability to kill S. aureus. Both bacteria are nasal colonizers. The present investigation looks into the antibiotic susceptibility pattern of co-habitation of S. lugdunensis with methicillin and vancomycin resistant Staphylococcus aureus in laboratory bred Wister rats. Nasal swabs of anaesthetized rats were collected using a sterile cotton swab moistened in 0.9% saline solution. All swabs were inoculated into nutrient broth, cultured at 37°C for 24 hrs. Overnight bacterial growth plated on blood agar and incubated at 37°C for 24 hrs. Organism identification and antibiotic susceptibility test were by using BioMerieux VITEK 2 compact automated system (BioMerieux, Marcy I’Etoile France), according to the manufacturers guidelines. Results obtained showed co-habitation of S. aureus with co-agulase negative bacteria, inclusive of S. lugdunensis. All the isolates were resistant to methicillin with a 33.3% resistance to vancomycin. The difference between the number of antibiotic resistant or sensitive varied statistically among the Staphylococcal isolates. For S. aureus 1, the difference was significant with p-value 0.034 but not significant for isolates 2, 3 and 4 with p-values of 0.158, 0.477 and 0.158 respectively. A statistically significant difference was seen with S. lugdunensis. The result from the study therefore, showed that the colonization of the nasal snares of the laboratory bred rats with S. aureus and other co-agulase negative Staphylococci was not affected by the presence of S. lugdunensis. 展开更多
关键词 NASAL staphylococcus lugdunensis staphylococcus aureus methicillin VANCOMYCIN Resistance Rats
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Treatments and limitations for methicillin-resistant Staphylococcus aureus: A review of current literature
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作者 Rahul Kashyap Aditya Shah +3 位作者 Taru Dutt Patrick M Wieruszewski Jaishid Ahdal Rishi Jain 《World Journal of Clinical Infectious Diseases》 2019年第1期1-10,共10页
Methicillin-resistant Staphylococcus aureus(MRSA)has remained a major threat to healthcare;in both hospital and community settings over the past five decades.With the current use of antibiotics for a variety of infect... Methicillin-resistant Staphylococcus aureus(MRSA)has remained a major threat to healthcare;in both hospital and community settings over the past five decades.With the current use of antibiotics for a variety of infections,including MRSA,emerging resistance is a major concern.Currently available treatments have restrictions limiting their use.These issues include,but are not limited to,side effects,cross-resistance,lack of understanding of pharmacokinetics and clinical pharmacodynamics,gradual increment in minimal inhibitory concentration over the period(MIC creep)and ineffectiveness in dealing with bacterial biofilms.Despite availability of various therapeutic options for MRSA,the clinical cure rates remain low with high morbidity and mortality.Given these challenges with existing treatments,there is a need for development of novel agents for MRSA.Along with prompt infection control strategies and strict implementation of antibiotic stewardship,cautious use of newer anti-MRSA agents will be of utmost importance.This article reviews the treatments and limitations of MRSA management and highlights the future path. 展开更多
关键词 methicillin resistant methicillin-resistant staphylococcus aureus ANTIBIOTICS MONOTHERAPY
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Molecular study on methicillin-resistant Staphylococcus aureus strains isolated from dogs and associated personnel in Jordan
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作者 Yaser Hamadeh Tarazi Ahmed Mahmoud Almajali +2 位作者 Mustafa Mohammad Kheer Ababneh Humam Shawket Ahmed Adnan Saleem Jaran 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2015年第11期902-908,共7页
Objective: To determine the prevalence, genetic relatedness, and pattern of antimicrobial susceptibility in methicillin-resistant Staphylococcus aureus(S. aureus)(MRSA) isolated from household dogs, farm dogs, and str... Objective: To determine the prevalence, genetic relatedness, and pattern of antimicrobial susceptibility in methicillin-resistant Staphylococcus aureus(S. aureus)(MRSA) isolated from household dogs, farm dogs, and stray dogs, compared to isolates from their associated personnel.Methods: MRSA was isolated from 250 nasal swabs(150 swabs from dogs and 100 swabs from humans). PCR assays were used to detect the presence of both the nuc and mec A genes,which con firmed the identity of S. aureus isolates and the presence of methicillin resistance,respectively. Disk diffusion was used to determine the antibiotic susceptibility against 15 antimicrobial agents along with an E-test that determined the minimum inhibitory concentration for oxacillin. Pulsed field gel electrophoresis was conducted to determine the genetic relatedness of MRSA isolates from dogs to those from associated and unassociated personnel.Results: The prevalence of S. aureus in dogs and humans was 12.7% and 10.0%respectively, while the prevalence of MRSA isolates in dogs and humans was 5.3% and5.0%, respectively. The prevalence of MRSA isolates in household dogs, farm dogs, and stray dogs was 7.8%, 4.7%, and 0.0%, respectively. MRSA isolates demonstrated a significantly higher rate of multi-resistance against three or more antimicrobial agents than methicillin-susceptible S. aureus(MSSA). Trimethoprim-sulphamethoxazole and chloramphenicol were the most effective antibiotics against all MRSA isolates. Pulsed field gel electrophoresis revealed a strong association between dog MRSA isolates and MRSA isolates from strongly associated personnel.Conclusions: MRSA is prevalent in house dogs, as well as in dog rearing centers and among their strongly associated personnel. A strong association was found between the MRSA isolates from dogs and those from humans who are in close contact. In addition,MRSA isolates showed a high rate of multi-resistance compared to MSSA isolates. 展开更多
关键词 staphylococcus aureus methicillin-resistant Staphy
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Crohn's disease complicated by intestinal infection with methicillin-resistant Staphylococcus aureus
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作者 Dominik Bettenworth Tobias M Nowacki +3 位作者 Alexander Friedrich Karsten Becker Johannes Wessling Jan Heidemann 《World Journal of Gastroenterology》 SCIE CAS 2013年第27期4418-4421,共4页
We report on a 24-year-old male patient with history of bloody diarrhea, abdominal pain and vomiting. Endoscopy revealed massive ulcerative discontinuous proctosigmoiditis with deep, sharply demarcated epithelial denu... We report on a 24-year-old male patient with history of bloody diarrhea, abdominal pain and vomiting. Endoscopy revealed massive ulcerative discontinuous proctosigmoiditis with deep, sharply demarcated epithelial denudations and enterotoxigenic methicillin-resistant Staphylococcus aureus (MRSA) was detected in mucosal biopsies. After treatment with linezolide and steroids, a significant amelioration of colitis was detected and testing for MRSA became negative. In face of the case presented here, we suggest that in patients with refractory inflammatory bowel disease (IBD), microbiological assessment should be performed to detect a possible Staphylococcus aureus infection in order to initiate an antimicrobial treatment in addition to IBDspecific treatment. 展开更多
关键词 Inflammatory BOWEL DISEASE Crohn’s DISEASE INFECTIOUS colitis staphylococcus aureus methicillin-resistant staphylococcus aureus
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Methicillin Resistant Staphylococcus aureus in Pigs and Pig Breeders in Three Regions of Cameroon
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作者 Cecile Ingrid Djuikoue Dorine Ekeu Ngatcheu +8 位作者 Olivier Pomte Melissa Julie Gonfouli Euphrasie Tsono Kemegne Charnelle Kemo Mouafo Andrea Sheisley Tchouotou I Didi Vynnie Manuella Nyatchoutou Cedric Seugnou Nana Ruffin Apalata Venant Tckokonte-Nana 《Open Journal of Medical Microbiology》 CAS 2022年第4期129-140,共12页
The spread of antibiotic resistance, whether in the community or in the hospital, has recently become a major public health problem. Moreover, livestock seems to be a reservoir of resistant microorganisms such as porc... The spread of antibiotic resistance, whether in the community or in the hospital, has recently become a major public health problem. Moreover, livestock seems to be a reservoir of resistant microorganisms such as porcine methicillin resistant Staphylococcus aureus (P-MRSA) whose carriage and transmission was mainly demonstrated in persons with occupational exposure to pigs. Much uncertainty remains about the public health implications of P-MRSA. To address concerns that exist regarding the zoonotic risk that pig carriers pose to breeders, the prevalence of P-MRSA in pigs and pig breeders was determined among 152 pig breeders in three regions of Cameroon. Materials and Method: Participants in this study came from Adamawa, Far North and Littoral regions of Cameroon. A total of 152 pig breeders or farm workers participated in this study. After having collected some social and cultural data with the aid of a questionnaire, nasal swabs were collected from pigs (n = 275) and their breeders using the standard collection procedures and placed in cooler containing cold accumulators. For each sample, microbiological assays were done as well as antibiotic susceptibility tests. Results: MRSA was isolated from 25 out of 275 pigs sampled (9.09%) and from 32 out of 152 (21.05%) pig breeders sampled;33 breeders (21.71%) were carriers of S. aureus including 32 MRSA and one MSSA. The prevalence of MRSA nasal carriage was 21.05% ± 6.48. A significant negative association between body protection and nasal carriage of MRSA (OR = 0.29, 95% CI [0.093;0.93];p = 0.04) was observed. In each region, similar strains of MRSA were isolated both in pigs and their breeders with the same antibiotic resistant profile. Conclusion: The prevalence of MRSA though not high compared to European countries warrants further research as data on this zoonosis is scarce in our context. As such, transmission of MRSA from pigs to pig breeders or vice-versa constitutes a real danger, and this relationship may be a starting point for MRSA contamination in the community. Moreover, proper use of body protections and antibiotic medications as recommended will be a better protective measure against nasal MRSA carriage. 展开更多
关键词 methicillin resistant staphylococcus aureus mrsa Pig Breeders PIGS Cameroon
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