To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith （AGS）, the crystal structure was measured with a Bruker APEX-Ⅱ area-...To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith （AGS）, the crystal structure was measured with a Bruker APEX-Ⅱ area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ （ECHS） was compared with these of traditional methods. The crystal belongs to the monoclinic system, space group P2b with unit cell parameters： a=（8.3652±0.0006） nm, b=（24.721±0.002） nm, and c=（14.5587±0.0011） nm, α=90°, β=97.850 （4） °, γ=90 °, V=2982.51 nm3, Dc= 1.179 mg/m3, and the molecular number （Z） of elementary structures was 2. The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods. Then, central composite design-response surface methodology （CCD-RSM） was adopted for optimizing the extraction rate of ECHS methods. The optimized values of extraction parameters are as follows： for the for extraction process of acid hydrolysis are that extraction time 110.8 min, solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min, solvent-herb ratio 8.7 and the alkali content 8.79%. Finally, the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%.展开更多
The recombinant expression of phycocyanobilin(PCB)was carried out in Escherichia coli,and the best fermentation conditions of recombinant E.coli biosynthesized PCB are optimized in the response surface methodology to ...The recombinant expression of phycocyanobilin(PCB)was carried out in Escherichia coli,and the best fermentation conditions of recombinant E.coli biosynthesized PCB are optimized in the response surface methodology to improve PCB production.The recombinant PCB is extracted,isolated,and purified by methanol and chloroform extraction.Recombinant PCB is validated in UV-vis spectroscopy,high-pressure liquid chromatography,and mass spectrometry.In addition,the anti-oxidant activities of the recombinant PCB are determined.The best induction conditions that optimized by Design Expert 8.0 software include:lactose concentration 4 mmol/L,induction temperature 24.69℃,induction time 4.6 h,and induction duration 13.57 h,under which the PCB expression level reached approximately 13 mg PCB/L,which is more than four times of previously reported 3 mg PCB/L.The maximum absorption peak of the recombinant PCB is located at 680 nm with a high fluorescence intensity of 470 nm.The recombinant PCB has a good ability to scavenge 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radicals.展开更多
The aims of this study is to design and optimize the functioning of a full continuous combined process based on electrocoagulaion-adsorption on crude Tunisian clay to treat a real textile effluent.The clay characteriz...The aims of this study is to design and optimize the functioning of a full continuous combined process based on electrocoagulaion-adsorption on crude Tunisian clay to treat a real textile effluent.The clay characterization shows that the used clay is a rich-smectite clay.The response surface methodology(RSM)technique based on Box-Behnken design(BBD)was used to optimize the process.At optimum conditions which are initial pH solution of 8.24,effluent flow rate of 0.5 L·min^(-1),voltage of 70 V,and added suspension of clay flow rate of 100 ml·min^(-1) the achieved color,chemical oxygen demand(COD)and total suspended solid(TSS)removal efficiencies were respectively 96.87%,89.77%and 84.46%with0.75 USD·m^(-3) as total cost.The additional laboratory experiments at optimum conditions agree with the predicted results,which confirm the accuracy and the capability of RSM to predict results in the defined space.Finally the designed process could be a good eco-friendly alternative to treat and reuse wastewater in industrial process with reasonable cost.展开更多
Security weaknesses in web applications deployed in cloud architectures can seriously affect its data confidentiality and integrity.The construction of the procedure utilized in the static analysis tools of source cod...Security weaknesses in web applications deployed in cloud architectures can seriously affect its data confidentiality and integrity.The construction of the procedure utilized in the static analysis tools of source code security differs and therefore each tool finds a different number of each weakness type for which it is designed.To utilize the possible synergies different static analysis tools may process,this work uses a new method to combine several source codes aiming to investigate how to increase the performance of security weakness detection while reducing the number of false positives.Specifically,five static analysis tools will be combined with the designed method to study their behavior using an updated benchmark for OWASP Top Ten Security Weaknesses(OWASP TTSW).The method selects specific metrics to rank the tools for different criticality levels of web applications considering different weights in the ratios.The findings show that simply including more tools in a combination is not synonymous with better results;it depends on the specific tools included in the combination due to their different designs and techniques.展开更多
基金Project(11JJ2042)supported by the Natural Science Foundation of Hunan Province,ChinaProject supported by the "Twelfth Five-Year" Key Discipline of Hunan University of Chinese Medicine-Pharmaceutical Analysis Science,China+1 种基金Project(11K048)supported by the Innovation Platform and Open Foundation Program of Higher Colleges of Hunan Province,ChinaProject(K1207010-21)supported by the Changsha City Science and Technology Bureau Key Projects,China
文摘To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith （AGS）, the crystal structure was measured with a Bruker APEX-Ⅱ area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ （ECHS） was compared with these of traditional methods. The crystal belongs to the monoclinic system, space group P2b with unit cell parameters： a=（8.3652±0.0006） nm, b=（24.721±0.002） nm, and c=（14.5587±0.0011） nm, α=90°, β=97.850 （4） °, γ=90 °, V=2982.51 nm3, Dc= 1.179 mg/m3, and the molecular number （Z） of elementary structures was 2. The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods. Then, central composite design-response surface methodology （CCD-RSM） was adopted for optimizing the extraction rate of ECHS methods. The optimized values of extraction parameters are as follows： for the for extraction process of acid hydrolysis are that extraction time 110.8 min, solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min, solvent-herb ratio 8.7 and the alkali content 8.79%. Finally, the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%.
基金Supported by the National Natural Science Foundation of China(No.41906109)the Key Research and Development Program of Yantai(No.Y933021011)
文摘The recombinant expression of phycocyanobilin(PCB)was carried out in Escherichia coli,and the best fermentation conditions of recombinant E.coli biosynthesized PCB are optimized in the response surface methodology to improve PCB production.The recombinant PCB is extracted,isolated,and purified by methanol and chloroform extraction.Recombinant PCB is validated in UV-vis spectroscopy,high-pressure liquid chromatography,and mass spectrometry.In addition,the anti-oxidant activities of the recombinant PCB are determined.The best induction conditions that optimized by Design Expert 8.0 software include:lactose concentration 4 mmol/L,induction temperature 24.69℃,induction time 4.6 h,and induction duration 13.57 h,under which the PCB expression level reached approximately 13 mg PCB/L,which is more than four times of previously reported 3 mg PCB/L.The maximum absorption peak of the recombinant PCB is located at 680 nm with a high fluorescence intensity of 470 nm.The recombinant PCB has a good ability to scavenge 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radicals.
文摘The aims of this study is to design and optimize the functioning of a full continuous combined process based on electrocoagulaion-adsorption on crude Tunisian clay to treat a real textile effluent.The clay characterization shows that the used clay is a rich-smectite clay.The response surface methodology(RSM)technique based on Box-Behnken design(BBD)was used to optimize the process.At optimum conditions which are initial pH solution of 8.24,effluent flow rate of 0.5 L·min^(-1),voltage of 70 V,and added suspension of clay flow rate of 100 ml·min^(-1) the achieved color,chemical oxygen demand(COD)and total suspended solid(TSS)removal efficiencies were respectively 96.87%,89.77%and 84.46%with0.75 USD·m^(-3) as total cost.The additional laboratory experiments at optimum conditions agree with the predicted results,which confirm the accuracy and the capability of RSM to predict results in the defined space.Finally the designed process could be a good eco-friendly alternative to treat and reuse wastewater in industrial process with reasonable cost.
文摘Security weaknesses in web applications deployed in cloud architectures can seriously affect its data confidentiality and integrity.The construction of the procedure utilized in the static analysis tools of source code security differs and therefore each tool finds a different number of each weakness type for which it is designed.To utilize the possible synergies different static analysis tools may process,this work uses a new method to combine several source codes aiming to investigate how to increase the performance of security weakness detection while reducing the number of false positives.Specifically,five static analysis tools will be combined with the designed method to study their behavior using an updated benchmark for OWASP Top Ten Security Weaknesses(OWASP TTSW).The method selects specific metrics to rank the tools for different criticality levels of web applications considering different weights in the ratios.The findings show that simply including more tools in a combination is not synonymous with better results;it depends on the specific tools included in the combination due to their different designs and techniques.
