Solid-phase synthesis of Cu2MoS4 nanoparticles for degradation of methyl blue under a halogen-tungsten lamp

The Cu_2MoS_4 nanoparticles were prepared using a relatively simple and convenient solid-phase process, which was applied for the first time. The crystalline structure, morphology, and optical properties of Cu_2MoS_4 nanoparticles were characterized using X-ray diffraction, X-ray photoelectron spectroscopy, field emission scanning electron microscopy, and UV-vis spectrophotometry. Cu_2MoS_4 nanoparticles having a band gap of 1.66 eV exhibits good photocatalytic activity in the degradation of methylene blue, which indicates that this simple process may be critical to facilitate the cheap production of photocatalysts.

Magnetic field assisted Fenton reactions for the enhanced degradation of methyl blue

Magnetic field was tentatively introduced into Fenton reactions system for the degradation and discoloration of methyl blue as the represent of organic chemical dye, which was a bio-refractory organic pollutant in industry wastewater. It was found that under optimal Fenton reaction conditions, with the assistant of magnetic field in Fenton reactions, the degradation rate of methyl blue, the decomposition rate of H2O2 and the conversion rate of Fe^2＋ were accelerated, the extent of them would be improved by the increase of magnetic field intensity. Meanwhile, the mineralization of methyl blue （CODer） was improved by over 10% with magnetic field.

Equilibrium,Kinetics and Thermodynamics of the Adsorption of Methylene Blue onto a Metal-Organic Frameworks Material,Copper Coordination Polymer with Dithiooxamide

The equilibrium, kinetics and thermodynamics of the adsorption of methylene blue( MB) from aqueous solution onto copper coordination polymer with dithiooxamide( H2dtoaCu),one of the metal-organic frameworks( MOFs),were investigated in a batch adsorption system as a function of initial pH, adsorbent concentration, contact time, initial dye concentration, and temperature. The Langmuir, Freundlich, and DubininRadushkevich( D-R) isotherm models were used for modeling the adsorption equilibrium. It was found that Langmuir model yielded a much better fit than the Freundlich model under different temperatures. The maximum monolayer adsorption capacities of MB were 192. 98,229. 86,and 297. 38 mg /g at 298,308,and 318 K,respectively. The calculated mean adsorption energy( 8. 26-11. 04 kJ /mol) using D-R model indicated that the adsorption process might take place by chemical adsorption mechanism.Otherwise,the kinetic studies revealed that the adsorption process could be well explained by pseudo-second-order rate kinetics and intraparticle diffusion was not the rate-limiting step.Thermodynamic studies indicated that this system was feasible,spontaneous,and endothermic process. Based on these studies,H2dtoaCu can be considered as a potential adsorbent for the removal of MB from aqueous solution.

Adsorptive Behavior of Methyl Blue on Graphene Aerogel:A Mechanism Study

Graphene aerogel was synthesized and used for the removal of methyl blue from aqueous solutions.The effect of solution pH,temperature and adsorption time on the adsorption performance of the graphene aerogel was studied systematically.In addition,investigations were also performed to determine the nature of adsorption.The experimental results show that graphene aerogel is a highly efficient adsorbent for the treatment of methyl blue in aqueous solutions.In addition,the adsorption of methyl blue proceeds through a single layer physical adsorption on the graphene aerogel.The findings herein are useful for the future development of adsorbent for in water.

Kinetics and Thermodynamics of Adsorption Methylene Blue onto Tea Waste/CuFe₂O₄Composite

Tea waste/CuFe2O4 (TW/C) composite was prepared by co-precipitation method. The TW and TW/C samples are characterized by FTIR, XRD, SEM and N2 physical adsorption. The results showed that specific surface area of 350 and 570 m2·g?1 for TW and TW/C, respectively. The average pore size of TW/C is ca. 100 nm. Adsorption of methylen blue onto TW/C composite has been studied. Measurements are performed at various contact time, pH and adsorbent dosage. The adsorption kinetics of methylen blue (MB) could be described by the pseudo-second order kinetic model. The adsorption isotherms are described by means of Langmuir and Freundlich isotherms. It was found that the Freundlich model fit better than the Langmuir model. The thermodynamic constants of the adsorption were calculated to predict the nature of adsorption. The values of thermodynamic parameters indicate that a spontaneous and endothermic process was occurred.

Voltammetric studies on the interaction of amikacin with methyl blue and its analytical application

A new method to determine the concentration of amikacin （AMK） using methyl blue （MB） as electrochemical probe was developed in this paper. In pH 4.5 Britton-Robinson （B-R） buffer solution, the MB reacted with AMK to form ion association complexes, which led to the reductive peak current of MB at -0.275 V （versus SCE） to decrease, and the decreases were linear with the concentration of AMK in the range of 1.0-60.0 mg/L, the regression of equation is AIp （hA） = -8.48 ＋ 102.36c （rag/L）, correlation coefficient yis 0.997. The conditions for determining the concentration of AMK using linear sweep voltammetry （SLV） were optimized. The method was used to determine the content of amikacin commercially available with satisfactory results.

Optimizing Methylene Blue Removal from Textile Effluents: Comparative Study of Adsorption Efficiency Using Raw and Activated Carbon Derived from Gmelina Wood Wastes

This research investigates the efficacy of activated Gmelina Wood Sawdust (GWS) as an adsorbent for the removal of methylene blue (MB) dye from aqueous solutions, in comparison with raw GWS. The study employs laboratory experiments to assess the percentage of dye removal across various temperature and pH conditions. The adsorption process is scrutinized under different parameters, encompassing contact time, initial dye concentration, adsorbent dosage, temperature, and pH. Results demonstrate that activated GWS surpasses its raw counterpart, showcasing superior MB dye removal percentages. Extended contact times increased initial dye concentrations, and higher adsorbent dosages contribute positively to removal efficiency, while temperature exhibits an inverse relationship with dye removal. Optimal adsorption occurs at a pH of 7.0, aligning with the adsorbent’s zero-point charge (pHzpc), underscoring the role of surface charge in the adsorption process. This study underscores the potential of activated GWS as an economical and promising adsorbent material for addressing pollutants. Furthermore, the utilization of activated carbon derived from abundant agricultural waste underscores an environmentally conscious approach to adsorption applications. The ability to tailor the size and properties of activated carbon particles opens avenues for optimizing adsorption capabilities, thereby presenting opportunities for enhanced water treatment solutions.

Solution Blowing of Palygorskite-Based Nanofibers for Methylene Blue Adsorption

Palygorskite(PG)adsorbent with superior adsorption property and ion-exchange ability is highly desired in the field of dye removal.However,it generates high amounts of precipitation due to the granular form,resulting in secondary pollution after adsorption.Herein,the novel high porosity PG-based nanofibers that are easy for operating and retrieving have been fabricated using effective solution blowing and subsequent calcination.The obtained highly efficient adsorption nanofibers exhibit large specific surface area about 170.50 m^(2)/g with average diameter from 243 nm to 365 nm.Based on the abovementioned nanofibrous structure and negatively charged PG,the solution blowing of PG-based nanofibers(SBPNs)showed high adsorption capacity for methylene blue(MB)(112.36 mg/g).In addition,the adsorption of SBPNs is well described by the Langmuir isotherm model.This work provides new SBPNs forming process for the fields of dye removal,which may achieve the production of PG adsorbents at the industrial level.

Batch Studies for Methylene Blue Removal and Recovery by Untreated Coffee Residues

This paper reports the practicability of using coffee residues to remove widely used basic dyes like MB （Methylene Blue） from wastewaters. The effect of different batch system parameters, namely stirring speed, pH, initial dye concentration and contact time were studied. Moreover, in continuous fixed-bed column systems, the effects of parameters such as bed-depth, flow rate and initial dye concentration were examined. The experimental batch systems data were simulated using （a） Freundlich, Langmuir and Sips isotherm models and （b） 1st order, 2nd order, and intra-particle kinetic models. The results revealed that the MB is fairly adsorbed on coffee residues. This process could be a low cost technique for the removal of basic dyes from aqueous systems.

Investigation of Methylation Profiles of TP53, Caspase 9, Caspase 8, Caspase 3 Genes Treated with DNA Methyl Transferase Inhibitor (DNMTi) Zebularine (ZEB) and Caffeic Acid Phenethyl Ester (CAPE) on MCF-7 and MDA-MB-231 Breast Cancer Cell Lines

Purpose: MCF-7 (ER+, WTP53) and MDA-MB-231 (ER Met, Mutant P53) Caffeic Acid Phenethyl Ester (CAPE) and DNA Methyl Transferase Inhibitor (DNMTi) in breast cancer cell lines of Zebularine (ZEB) single and combined application of TP53, caspase-9, caspase 8 and caspase-3 genes as a result of the use of single and combined drug methylation profiles are aimed to be evaluated by specific PCR method. Material-Metods: In the MCF-7 and MDA-MB-231 breast cancer cell lines, MTT test and survival analysis were performed as a result of single and combined application of CAPE and Zebularine and Methylation Specific PCR was performed to examine the methylation of caspase-3, caspase-8, caspase-9 and TP53 genes. Results: According to the results of 24-hour drug administration, the IC50 for the MCF-7 cell line was determined as 200 μM, for CAPE 40 μM and for the combined values of 50 μM ZEB + 5 μM CAPE. The effects of caspase-3, caspase-8, caspase-9 and TP53 genes on the methylation level of ZEB, CAPE and ZEB + CAPE drug combination were determined by using bisulfite modified DNAs in MCF-7 and MDA-MB-231 cell lines. Discussion: In the MCF-7 cell line, the 120 μM ZEB viability rate was 51%, and the viability of 80 μM ZEB MDA-MB-231 breast cancer cells decreased by 59.7%. After 20 μM CAPE, viability in MCF-7 cells decreased by 31% in 120 μM CAPE and MDA-MB-231 cells decreased by 41%. The viability with 40 μM CAPE decreased by 19% in MDA-MB-231 cells. It was found that 20 μM CAPE concentration was associated with TP53 methylation in MCF-7 cell lines. The 80 μM ZEB concentration was found to be closely related to the unmethylated status of the TP53 gene. These results obtained with 50 μM ZEB + 5 μM CAPE application were found to be related to the methylated-unmetylated status of the TP53 gene in half (50%). For the caspase-9 gene of MDA-MB-231 cells, 80 μM ZEB concentration was found to be associated with unmetylated status. The effective use of drugs with low concentrations of the drug dose provides a more appropriate approach in terms of treatment.

超声波照射激活亚甲基蓝(MB)声动力损伤牛血清白蛋白(BSA)

应用紫外-可见(UV-Vis)光谱和荧光光谱研究了超声波激活亚甲基蓝(MB)对牛血清白蛋白(BSA)分子的损伤,考察了超声波照射时间,MB浓度,溶液酸度对BSA分子损伤的影响。结果表明,在一定条件下,BSA分子的损伤程度随着超声波照射时间,MB浓度和pH值增加而增加,即UV-Vis光谱表现出越来越明显的增色效应,荧光光谱表现出越来越明显的猝灭现象。探讨了超声波激活MB对BSA分子损伤的可能机理。

人工砂MB值对水泥砂浆性能的影响研究

在人工砂原岩开采及加工生产过程中混入的黏土矿物导致人工砂MB值超标,会对水泥砂浆某些重要性能造成负面影响。考虑不同黏土矿物物理化学特性差异大,研究人工砂MB值相同时不同黏土矿物对水泥砂浆性能影响的规律,对指导不同MB值人工砂的使用具有重要意义。通过使用膨胀性黏土蒙脱石或非膨胀性黏土伊利石,分别配制相同MB值、相同级配的人工砂,采用相同砂浆配合比,分别进行砂浆和易性、力学性能、抗冻性能、干燥收缩性能试验。结果表明,含相同MB值、不同黏土矿物类型的砂浆的性能存在明显差异。在人工砂具有相同MB值时,含蒙脱石砂浆与伊利石砂浆相比,和易性较差,但力学性能、抗冻和干燥收缩性能较好。

PVC-P(VC-VAC)-MBS共混超滤膜的制备及其性能

利用非溶剂致相转化法(NIPS)制备了PVC-P(VC-VAC)-MBS平板和中空纤维超滤膜,讨论了氯乙烯-醋酸乙烯共聚物[P(VC-VAC)]的含量对膜机械性能、亲水性、纯水通量及截留率等性能的影响。同时,通过添加抗冲击改性剂甲基丙烯酸甲酯-丁二烯-苯乙烯共聚物(MBS),考察了MBS对PVC平板超滤膜机械性能的影响.实验结果表明,当铸膜液中P(VCVAC)的质量分数为4%时,亲水性最好,纯水通量可由42.5L/(m2·h)增大到155L/(m2·h);同时,膜的机械性能明显增强,P(VC-VAC)和MBS树脂可以有效地改善PVC超滤膜的亲水性和机械性能,是优良的PVC膜共混改性材料.

5-Aza-CdR对MDA-MB-231细胞PPAR-γ基因甲基化的影响

目的研究去甲基化药物5-氮杂-2-脱氧胞嘧啶(5-aza-2.-deoxycytidine,5-Aza-CdR)对三阴性乳腺癌(triple-negative breast cancer,TNBC)细胞MDA-MB-231凋亡及过氧化物酶体增殖物激活受体-γ(peroxisome proliferator-activated receptor-γ,PPAR-γ)基因表达的影响。方法 5-Aza-CdR作用MDA-MB-231细胞后,流式细胞术检测MDA-MB-231细胞凋亡,实时荧光定量PCR(real-time fluorescent quantive PCR,q PCR)检测PPAR-γ m RNA的表达,甲基特异性PCR(methylmion specific PCR,MSP)检测PPAR-γ甲基化的改变。结果流式细胞结果表明,随着药物浓度增加,细胞处理48 h后,凋亡率增加,呈剂量依赖性。5、10、15和20μmol/L组凋亡率分别为(14.1±2.3)%、(25.4±3.3)%、(32.7±2.8)%、(43.1±1.9)%,与对照组的(6.9±0.8)%相比差异有统计学意义(P<0.05);q PCR结果显示,随着药物浓度增加,PPAR-γ m RNA表达上调;MSP显示PPAR-γ甲基化水平降低。结论 5-Aza-CdR能降低PPAR-γ甲基化状态,使PPAR-γ表达增加,促进MDA-MB-231细胞凋亡。PPAR-γ甲基化水平改变可能是引起MDA-MB-231细胞生物学改变的机制之一。

一锅法合成纳米复合材料MB@ZIF-67及其表征研究

采用一锅法将染料亚甲基蓝(MB)成功的组装入类沸石咪唑酯骨架结构材料Co(mIm)_2(ZIF-67)的孔道中,合成了纳米复合材料MB@ZIF-67,并对其进行了表征。同时研究了ZIF-67和MB@ZIF-67对染料甲基橙(MO)的吸附,结果表明:两者对MO的最大吸附量分别为12.8和15.5mg/g;最大去除率分别为63.7%和77.5%。吸附过程是ZIF-67骨架中的钴离子与MO分子中—SO_3^-之间的静电引力引起的。同时二者的吸附量相差2.7mg/g,说明吸附过程中MB@ZIF-67与MO之间存在较大的范德华力。

分光光度法测定砂MB值的试验研究

试验用分光光度法测定砂MB值的可行性。对比传统色晕法和分光光度法测定的结果,证明分光光度法测得的MB值较色晕法略高,但其测量精度优于色晕法。两种方法测得的结果存在强烈的相关性,可采用回归的公式进行相互换算。

用于促黄体生成素电化学传感器的MWCNT/MB/AuNP纳米复合材料

制备了一种用于促黄体生成素(LH)电化学传感器的多壁碳纳米管/亚甲基蓝/金纳米颗粒(MWCNT/MB/AuNP)纳米复合材料。利用光学显微镜(OM)、扫描电子显微镜(SEM)和X射线能量色散谱仪(EDS)对制备的纳米复合材料进行形貌表征。采用方波伏安(SWV)法和循环伏安(CV)法对AuNP的催化能力、纳米复合材料合成的最优化比例和氧化还原性能进行测试;对利用纳米复合材料制备的LH电化学传感器进行SWV测试。实验结果表明该纳米复合材料具有良好的氧化还原性能;利用纳米复合材料制备的电化学传感器具有良好的重复性和较宽的线性范围,能够在3.5~140 mIU/mL浓度内进行LH的检测。该纳米复合材料可推广应用于其他类型电化学传感器的制备。

病理性黄疸新生儿蓝光照射疗效与血清α-GST、CK-MB的关系

目的:探讨病理性黄疸新生儿蓝光照射疗效与血清α-谷胱甘肽S转移酶(α-GST)、肌酸激酶同工酶MB(CK-MB)水平的关系。方法:选取2019年1月至2020年12月期间洛阳市妇幼保健院、解放军联勤保障部队第989医院收治的92例病理性黄疸新生儿作为研究对象,所有患儿均采取蓝光照射治疗,在治疗前测定血清α-GST、CK-MB水平,并在治疗7 d后统计患儿蓝光照射治疗无效的发生情况,并分析血清α-GST、CK-MB水平与患儿蓝光照射疗效的关系。结果:92例病理性黄疸新生儿蓝光照射治疗后,治疗有效共68例,治疗有效率为73.91%,治疗无效共24例,无效率26.09%;治疗无效患儿的血清α-GST、CK-MB水平均高于治疗有效患儿,差异有统计学意义(P<0.05);经logistic回归分析,血清α-GST、CK-MB水平是患儿蓝光照射治疗无效的危险因素。结论:患儿蓝光照射治疗无效的可能性较高,血清α-GST、CK-MB水平与病理性黄疸新生儿蓝光照射疗效具有密切关联性。

High Efficient Photocatalytic Degradation of 3,7-Bis(Dimethylamino)-Phenothiazin-5-Ium Chloride Dye and Kinetics of H2 Evolution of N2H4H2O by Synthesized CdS/NiS Nanocomposite by Electrochemical Method

CdS/NiS nanocomposites were synthesized by electrochemical method. Ni and Cd is one of the important II-VI semiconducting materials with a direct band gap of 3.26 eV which finds applications in electrical conductivity and photo-catalysis. The synthesized nanocomposites were characterized by BET, UV-VIS, XRD, FE-SEM (EDAX) techniques. X-Ray diffraction (XRD) reveals crystallite size to be 23.22 nm which was calculated using Williamson-Hall (W-H) plot method. The energy of the band gap for CdS/NiS could be thus estimated to be 3.26 eV. The photocatalytic activity of the sample was evaluated by the degradation of textile dye methylene Blue (MB) in aqueous solutions under UV radiation. Hydrogen energy is regarded as a promising alternative in terms of energy conversion and storage. Hydrogen Evolution Reaction (HER) was carried out in both visible light and UV light by using Hydrazine (N2H4H2O) in the presence of CdS/NiS nanocomposite. The synthesized photocatalyst shows applicable performance for kinetics of Hydrogen Evolution Reaction (HER) in Visible light and UV light. The decomposition of hydrazine (N2H4H2O) proceeded rapidly to generate free hydrogen rich gas through OH radical contact with CdS/NiS nanocomposite at room temperature. The rate of HER is limited by either proton adsorption onto an active site or evolution of formed hydrogen from the surface. A high Tafel slope is indicative of proton adsorption as the rate limiting step, while a lower Tafel slope (20 - 45 mV) indicates that the evolution of molecules hydrogen from the catalyst is rate limiting. In the present case the Tafel slopes for visible light 23.5 mV and 42.5 mV for UV light. Blank experiments show poor activity for HER i.e. 10.1 - 13.5 mV.

外源茉莉酸甲酯诱导脐橙果实抗青霉病与活性氧代谢的关系

【目的】通过探究外源茉莉酸甲酯(Methyl Jasmonate,MeJA)诱导脐橙果实采后抗青霉病与活性氧代谢的关系,为MeJA应用于控制果实采后病害提供理论依据。【方法】以纽荷尔和龙回红脐橙果实为试材,在温度(26±1)℃、湿度85%~95%的环境下,使用浓度为50μmol·L^(-1) MeJA密闭熏蒸处理24 h后,接种青霉病菌Penicillium italicum(1.25×10^(6) spores·mL^(-1))20µL,以无菌水处理为对照,另设100μmol·L^(-1)水杨苷异羟肟酸(salicyhydroxamic acid,SHAM)喷施处理,测定脐橙果实活性氧代谢相关指标并分析关键酶基因表达水平。【结果】50μmol·L^(-1) MeJA熏蒸处理显著提高2个脐橙品种果实的谷胱甘肽还原酶(GR)、抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、单脱氢抗坏血酸还原酶(MDHAR)和脱氢抗坏血酸还原酶(DHAR)活性以及对应酶基因CsGR、CsAPX、Cu-ZnSOD、CsCAT、CsMDHAR、CsDHAR3的相对表达量,在纽荷尔中MeJA处理分别为对照的1.10、1.16、1.12、1.69、1.21、1.43、1.16、1.42、1.56、1.36、1.58、2.32倍,在龙回红中MeJA处理分别为对照的1.13、1.49、1.02、1.41、1.26、1.79、2.98、1.59、1.10、1.79、1.57、1.31倍;同时,显著提高了还原型谷胱甘肽(GSH)、抗坏血酸(AsA)含量和DPPH自由基清除率,降低了过氧化氢(H2O2)含量和超氧阴离子(O2.-)产生速率,延缓了丙二醛(MDA)含量的积累。【结论】使用MeJA于接种前24 h熏蒸脐橙果实,能提高柑橘果实活性氧代谢相关酶活性以及关键酶基因的表达量,增强果实对青霉病的抗性。