Objective：The role of methylseleninic acid （MSeA）, a selenium compound, has been documented in cancer chemoprevention. However, the therapeutic effect of MSeA in combination with paclitaxel, a chemotherapeutic agen...Objective：The role of methylseleninic acid （MSeA）, a selenium compound, has been documented in cancer chemoprevention. However, the therapeutic effect of MSeA in combination with paclitaxel, a chemotherapeutic agent used to treat ovarian cancer, is unknown. In this study, we investigated the effect of combination treatment of MSeA and paclitaxel against ovarian cancer cells. Methods：Ovarian cancer cells（A2780） were treated with different concentrations of MSeA, paclitaxel alone or in combination. The individual and combined concentrations of drugs that achieved certain cells growth/death were determined using a sulforhodamine B（SRB） assay. Drug effects on cell viability were further confirmed using floating cell count and trypan blue exclusion assay. The mean Values ± standard deviation were calculated and compared between treatment groups using unpaired t test. Results： The concentration of paclitaxel alone that inhibited 50% of cell growth（IC50） was 0.5 μmol/L. This concentration increased to 1.2 μmol/L when paclitaxel was given in sequential combination with MSeA. The number of dead cells after the combination treatment did not show a significance increase when compared with drug alone. Conclusion：Pretreatment with MSeA did not enhance the paclitaxel effect against A2780 ovarian cancer cells.展开更多
Background and Objective Lung cancer has become the most frequent malignant cancer which threatens human health and life in the world. Although progresses have been made in
Objective and Methods Lung cancer has a fastest growing rate of morbidity and mortality among malignant tumors and poses a great threat to the human health. Chemotherapy, as one
Background and Objective The disease incidence and mortality of lung cancer has been increased dramatically for recent 50 years in many countries in the world. In2002, the new cases
Background and Objective Lung cancer has the fastest increasing rate of morbidity and mortality all over the world and appears to be one of the most dangerous malignant tumors
Background and Objective Lung cancer is the rst killer of human being in the whole world. Recently, although many treatment strategies have been developed, the anti-cancer effects
Background and Objective Lung cancer, which has been proved to have fastest increasing rate of morbidity and mortality, appears to be one of the most dangerous malignant tumor that
Methanesulphonate was investigated as a potential contributor to the sulphur budget based on the analysis on Antarctic snow/ice from Coffins Ice Cap, King George Island (62°10’ S, 58°50,W). The anion was fo...Methanesulphonate was investigated as a potential contributor to the sulphur budget based on the analysis on Antarctic snow/ice from Coffins Ice Cap, King George Island (62°10’ S, 58°50,W). The anion was found to be present at a mean concentration of 0. 17 μeq. L-1 with a maximum of 0.73 μeq. L-1. A distinct seasonal variation exists in the top 10 m of the core, equivalent to more than two years of deposition. Dating resulted from δ18O profile suggests that the principal peaks of methanesulphonate are associated with snow deposited in autumn and secondary peaks in spring. The seasonal patterns of methanesulphonate in the subantarctic snow displays a phase difference from that observed in marine air from low and middle latitudes.展开更多
基金supported in part by a research grant form the American Cancer Society(IRG0219706)a comprehensive cancer center support grant from the National Cancer Institute(CA016056)
文摘Objective：The role of methylseleninic acid （MSeA）, a selenium compound, has been documented in cancer chemoprevention. However, the therapeutic effect of MSeA in combination with paclitaxel, a chemotherapeutic agent used to treat ovarian cancer, is unknown. In this study, we investigated the effect of combination treatment of MSeA and paclitaxel against ovarian cancer cells. Methods：Ovarian cancer cells（A2780） were treated with different concentrations of MSeA, paclitaxel alone or in combination. The individual and combined concentrations of drugs that achieved certain cells growth/death were determined using a sulforhodamine B（SRB） assay. Drug effects on cell viability were further confirmed using floating cell count and trypan blue exclusion assay. The mean Values ± standard deviation were calculated and compared between treatment groups using unpaired t test. Results： The concentration of paclitaxel alone that inhibited 50% of cell growth（IC50） was 0.5 μmol/L. This concentration increased to 1.2 μmol/L when paclitaxel was given in sequential combination with MSeA. The number of dead cells after the combination treatment did not show a significance increase when compared with drug alone. Conclusion：Pretreatment with MSeA did not enhance the paclitaxel effect against A2780 ovarian cancer cells.
基金supported by a grant from the key project of the National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30430300)National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30670922)INTERNATION Scienc and Techniquie COOPRATION PROGRAM OF CHINA (ISCP) (to Qinghua ZHOU)(No.2006DFB32330)
文摘Background and Objective Lung cancer has become the most frequent malignant cancer which threatens human health and life in the world. Although progresses have been made in
基金supported by the grants from the Key Project of National Natural Science Foundation of China (No.30430300 , to Qinghua ZHOU)Key Projects of Tianjin Sci-Tech Support Program (No. 07SYSYSF05000 and No. 06YF-SZSF05300, to Qinghua ZHOU)
文摘Objective and Methods Lung cancer has a fastest growing rate of morbidity and mortality among malignant tumors and poses a great threat to the human health. Chemotherapy, as one
基金supported by a grant from the key project of the National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30430300)National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30670922)INTERNATION Scienc and Techniquie COOPRATION PROGRAM OF CHINA (ISCP) (to Qinghua ZHOU)(No.2006DFB32330)
文摘Background and Objective The disease incidence and mortality of lung cancer has been increased dramatically for recent 50 years in many countries in the world. In2002, the new cases
基金supported by a grant from the key project of the National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30430300)National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30670922)INTERNATION Scienc and Techniquie COOPRATION PROGRAM OF CHINA (ISCP) (to Qinghua ZHOU)(No.2006DFB32330)
文摘Background and Objective Lung cancer has the fastest increasing rate of morbidity and mortality all over the world and appears to be one of the most dangerous malignant tumors
基金supported by a grant from the key project of the National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30430300)National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30670922)INTERNATION Scienc and Techniquie COOPRATION PROGRAM OF CHINA (ISCP) (to Qinghua ZHOU)(No.2006DFB32330)
文摘Background and Objective Lung cancer is the rst killer of human being in the whole world. Recently, although many treatment strategies have been developed, the anti-cancer effects
基金supported by a grant from the key project of the National Natural Science Foundation of China (to QinghuaZHOU)(No. 30430300)National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30670922)INTERNATION Scienc and Techniquie COOPRATION PROGRAM OF CHINA (ISCP) (to Qinghua ZHOU)(No.2006DFB32330)
文摘Background and Objective Lung cancer, which has been proved to have fastest increasing rate of morbidity and mortality, appears to be one of the most dangerous malignant tumor that
文摘Methanesulphonate was investigated as a potential contributor to the sulphur budget based on the analysis on Antarctic snow/ice from Coffins Ice Cap, King George Island (62°10’ S, 58°50,W). The anion was found to be present at a mean concentration of 0. 17 μeq. L-1 with a maximum of 0.73 μeq. L-1. A distinct seasonal variation exists in the top 10 m of the core, equivalent to more than two years of deposition. Dating resulted from δ18O profile suggests that the principal peaks of methanesulphonate are associated with snow deposited in autumn and secondary peaks in spring. The seasonal patterns of methanesulphonate in the subantarctic snow displays a phase difference from that observed in marine air from low and middle latitudes.
