Background:Myocardial infarction(MI)is known worldwide for its important disabling features,including myocarditis and cardiomyocyte apoptosis.It is believed that microRNA(miRNA)has a role in the cellular processes of ...Background:Myocardial infarction(MI)is known worldwide for its important disabling features,including myocarditis and cardiomyocyte apoptosis.It is believed that microRNA(miRNA)has a role in the cellular processes of apoptosis and myocarditis,and miR-219a-5p has been found to suppress the inflammatory response.However,unknown is the precise mechanism by which miR-219a-5p contributes to MI.Methods:We measured the expression of miR-219a-5p and evaluated its effects on target proteins,inflammatory factors,and apoptosis in a mouse model of MI.Echocardiography was utilized to examine the MI clinical index,and triphenyl tetrazolium chloride staining was employed to analyze the infarcted region.Enzyme-linked immunosorbent assay and Western blotting measured serum and molecular markers in heart tissues.To quantify the association with miR-219a-5p and ATPase sarcoplasmic/endoplasmic reticulum Ca^(2+) transporting 2(ATP2A2),the luciferase activity assay and Pearson’s correlation analysis were employed.Results:MiR-219a-5p exhibited low expression in a mouse model of MI,and its amplification prevented both apoptotic and inflammatory reactions.Specifically,miR-219a-5p targeted ATP2A2.Conclusion:In a mouse model of MI,miR-219a-5p exerted a potent protective effect via direct targeting of ATP2A2.展开更多
[目的]分析mi R-196a-2基因多态位点rs11614913与乳腺癌易感性的关系。[方法]检索外文数据库Pubmed、Science Direct和中文数据库CNKI、万方,收集截止到2016年5月31日发表的关于mi R-196a-2基因多态位点rs11614913与乳腺癌易感关系的病...[目的]分析mi R-196a-2基因多态位点rs11614913与乳腺癌易感性的关系。[方法]检索外文数据库Pubmed、Science Direct和中文数据库CNKI、万方,收集截止到2016年5月31日发表的关于mi R-196a-2基因多态位点rs11614913与乳腺癌易感关系的病例对照研究,按纳入与排除标准筛选文献,利用Meta分析的方法对各研究数据进行统计学处理及异质性检验,评估发表偏倚并进行敏感性分析。[结果]共纳入15篇研究mi R-196a-2基因多态位点rs11614913与乳腺癌易感关系的文献,包括6362例病例和7392例对照,结果显示：等位基因模型（T vs C）、隐形模型（TT vs CC＋CT）和相加模型（TT vs CC）与降低乳腺癌发病风险相关,差异均有统计学意义（OR=0.89,95%CI：0.81~0.99;OR=0.83,95%CI：0.72~0.96;OR=0.79,95%CI：0.65~0.97）。按种族进行亚组分析后发现,在亚洲人群中,等位基因模型（T vs C）、显性模型（TT＋CT vs CC）、隐形模型（TT vs CC＋CT）和相加模型（TT vs CC）也与降低乳腺癌发病风险相关（OR=0.85,95%CI：0.75~0.95;OR=0.83,95%CI：0.70~0.99;OR=0.78,95%CI：0.67~0.91;OR=0.72,95%CI：0.57~0.91）,其余模型均不能认为与乳腺癌的易感性相关。高加索人群的各遗传模型均与乳腺癌的发病风险无显著相关性。[结论]mi R-196a-2基因多态位点rs11614913的T等位基因和TT基因型可能与降低乳腺癌的发病风险相关。展开更多
基金supported by the National Nature Science Foundation of the People’s Republic of China(No.81400225 for Zulong Sheng and No.82000382 for Yanru He)the Jiangsu Provincial Medical Youth Talent(No.QNRC2016815).
文摘Background:Myocardial infarction(MI)is known worldwide for its important disabling features,including myocarditis and cardiomyocyte apoptosis.It is believed that microRNA(miRNA)has a role in the cellular processes of apoptosis and myocarditis,and miR-219a-5p has been found to suppress the inflammatory response.However,unknown is the precise mechanism by which miR-219a-5p contributes to MI.Methods:We measured the expression of miR-219a-5p and evaluated its effects on target proteins,inflammatory factors,and apoptosis in a mouse model of MI.Echocardiography was utilized to examine the MI clinical index,and triphenyl tetrazolium chloride staining was employed to analyze the infarcted region.Enzyme-linked immunosorbent assay and Western blotting measured serum and molecular markers in heart tissues.To quantify the association with miR-219a-5p and ATPase sarcoplasmic/endoplasmic reticulum Ca^(2+) transporting 2(ATP2A2),the luciferase activity assay and Pearson’s correlation analysis were employed.Results:MiR-219a-5p exhibited low expression in a mouse model of MI,and its amplification prevented both apoptotic and inflammatory reactions.Specifically,miR-219a-5p targeted ATP2A2.Conclusion:In a mouse model of MI,miR-219a-5p exerted a potent protective effect via direct targeting of ATP2A2.
文摘[目的]分析mi R-196a-2基因多态位点rs11614913与乳腺癌易感性的关系。[方法]检索外文数据库Pubmed、Science Direct和中文数据库CNKI、万方,收集截止到2016年5月31日发表的关于mi R-196a-2基因多态位点rs11614913与乳腺癌易感关系的病例对照研究,按纳入与排除标准筛选文献,利用Meta分析的方法对各研究数据进行统计学处理及异质性检验,评估发表偏倚并进行敏感性分析。[结果]共纳入15篇研究mi R-196a-2基因多态位点rs11614913与乳腺癌易感关系的文献,包括6362例病例和7392例对照,结果显示：等位基因模型（T vs C）、隐形模型（TT vs CC＋CT）和相加模型（TT vs CC）与降低乳腺癌发病风险相关,差异均有统计学意义（OR=0.89,95%CI：0.81~0.99;OR=0.83,95%CI：0.72~0.96;OR=0.79,95%CI：0.65~0.97）。按种族进行亚组分析后发现,在亚洲人群中,等位基因模型（T vs C）、显性模型（TT＋CT vs CC）、隐形模型（TT vs CC＋CT）和相加模型（TT vs CC）也与降低乳腺癌发病风险相关（OR=0.85,95%CI：0.75~0.95;OR=0.83,95%CI：0.70~0.99;OR=0.78,95%CI：0.67~0.91;OR=0.72,95%CI：0.57~0.91）,其余模型均不能认为与乳腺癌的易感性相关。高加索人群的各遗传模型均与乳腺癌的发病风险无显著相关性。[结论]mi R-196a-2基因多态位点rs11614913的T等位基因和TT基因型可能与降低乳腺癌的发病风险相关。
