BACKGROUND Studies have shown that long non-coding RNAs(lncRNAs)play a key role in almost all key physiological and pathological processes,including different types of malignant tumors.Our previous lncRNA microarray r...BACKGROUND Studies have shown that long non-coding RNAs(lncRNAs)play a key role in almost all key physiological and pathological processes,including different types of malignant tumors.Our previous lncRNA microarray results have shown that lncRNA XLOC_001659 is upregulated in esophageal cancer(EC)tissues,with a fold change of 20.9 relative to normal esophageal tissues.But its effect and the molecular biological mechanisms on proliferation and invasion of EC cells remain unclear.AIM To investigate the effect of lncRNA XLOC_001659 on esophageal squamous cell carcinoma(ESCC)cells and explore the molecular biological mechanisms involved.METHODS RT-qPCR assay was used to quantify the expression levels of lncRNAXLOC-001659 and miR-490-5p.The proliferative capacity of the cells was determined using CCK8 and colony formation assays,and the effect of lncRNAXLOC-001659 on the invasion of ESCC cells was determined by Transwell assay.Dualluciferase reporter assay was used to detect the target genes of lncRNAXLOC-001659 and miR-490-5p.RESULTS The results of RT-qPCR showed that the expression of lncRNAXLOC_001659 was upregulated in ESCC cells.CCK-8 assay showed that knockdown of lncRNAXLOC_001659 significantly inhibited ESCC cell proliferation.Colony formation and Transwell invasion assays showed that knockdown of lncRNAXLOC_001659 or overexpression of miR-490-5p significantly inhibited ESCC cell growth and invasion.Furthermore,lncRNAXLOC_001659 acts as an endogenous sponge by competitively binding to miR-490-5p to downregulate miR-490-5p.Further results confirmed that miR-490-5p targeted PIK3CA,and the recovery of PIK3CA rescued lncRNAXLOC_001659 knockdown or miR-490-5p overexpression-mediated inhibition of cell proliferation and invasion,which suggested the presence of an lncRNAXLOC_001659/miR-490-5p/PIK3CA regulatory axis.CONCLUSION Knockdown of lncRNA XLOC_001659 inhibits proliferation and invasion of ESCC cells via regulation of miR-490-5p/PIK3CA,suggesting that it may play a role in ESCC tumorigenesis and progression.展开更多
基金Supported by Excellent Youth Development Fund of Zhengzhou University,No.1621328001
文摘BACKGROUND Studies have shown that long non-coding RNAs(lncRNAs)play a key role in almost all key physiological and pathological processes,including different types of malignant tumors.Our previous lncRNA microarray results have shown that lncRNA XLOC_001659 is upregulated in esophageal cancer(EC)tissues,with a fold change of 20.9 relative to normal esophageal tissues.But its effect and the molecular biological mechanisms on proliferation and invasion of EC cells remain unclear.AIM To investigate the effect of lncRNA XLOC_001659 on esophageal squamous cell carcinoma(ESCC)cells and explore the molecular biological mechanisms involved.METHODS RT-qPCR assay was used to quantify the expression levels of lncRNAXLOC-001659 and miR-490-5p.The proliferative capacity of the cells was determined using CCK8 and colony formation assays,and the effect of lncRNAXLOC-001659 on the invasion of ESCC cells was determined by Transwell assay.Dualluciferase reporter assay was used to detect the target genes of lncRNAXLOC-001659 and miR-490-5p.RESULTS The results of RT-qPCR showed that the expression of lncRNAXLOC_001659 was upregulated in ESCC cells.CCK-8 assay showed that knockdown of lncRNAXLOC_001659 significantly inhibited ESCC cell proliferation.Colony formation and Transwell invasion assays showed that knockdown of lncRNAXLOC_001659 or overexpression of miR-490-5p significantly inhibited ESCC cell growth and invasion.Furthermore,lncRNAXLOC_001659 acts as an endogenous sponge by competitively binding to miR-490-5p to downregulate miR-490-5p.Further results confirmed that miR-490-5p targeted PIK3CA,and the recovery of PIK3CA rescued lncRNAXLOC_001659 knockdown or miR-490-5p overexpression-mediated inhibition of cell proliferation and invasion,which suggested the presence of an lncRNAXLOC_001659/miR-490-5p/PIK3CA regulatory axis.CONCLUSION Knockdown of lncRNA XLOC_001659 inhibits proliferation and invasion of ESCC cells via regulation of miR-490-5p/PIK3CA,suggesting that it may play a role in ESCC tumorigenesis and progression.
