Objective: To verify the existence of microRNAs(miRNAs) extracted from fresh ginseng decoction.Methods: Fresh ginseng was prepared into decoction according to the conventional method. The miRNA were extracted from the...Objective: To verify the existence of microRNAs(miRNAs) extracted from fresh ginseng decoction.Methods: Fresh ginseng was prepared into decoction according to the conventional method. The miRNA were extracted from the condensed ginseng decoction by plant microRNA extraction kit. Then miRNA were treated by DNase I and subjected to agarose gel electrophoresis and Agilent 2100 bioanalysis. Mi R-159 and mi R-6135, which were highly expressed in ginseng, were selected and verified by real-time quantitative PCR to detect the expression in the decoction.Results: Ginseng miRNA were successfully extracted from fresh decoction. Mi R-159 and mi R-6135 were expressed in fresh decoction with lower levels than those of fresh ginseng.Conclusion: miRNAs stably existed after processing, and retained some stability after high-temperature treatment. The findings provide a valuables basis for the further studies on ginseng miRNAs.展开更多
基金supported by the National Natural Science Foundation for Young Scholars of China(no.81403195)Natural Science Foundation of Guangdong Province(no.S2013010015418)
文摘Objective: To verify the existence of microRNAs(miRNAs) extracted from fresh ginseng decoction.Methods: Fresh ginseng was prepared into decoction according to the conventional method. The miRNA were extracted from the condensed ginseng decoction by plant microRNA extraction kit. Then miRNA were treated by DNase I and subjected to agarose gel electrophoresis and Agilent 2100 bioanalysis. Mi R-159 and mi R-6135, which were highly expressed in ginseng, were selected and verified by real-time quantitative PCR to detect the expression in the decoction.Results: Ginseng miRNA were successfully extracted from fresh decoction. Mi R-159 and mi R-6135 were expressed in fresh decoction with lower levels than those of fresh ginseng.Conclusion: miRNAs stably existed after processing, and retained some stability after high-temperature treatment. The findings provide a valuables basis for the further studies on ginseng miRNAs.
