为进一步对大豆中的miR482家族有更全面的了解,本研究以大豆miR482家族为研究对象,对大豆中miR482家族的前体及成熟序列的特征、靶基因预测及其表达模式进行分析。在通过miRBase搜索得到的gma-miR482家族成熟序列中,除gma-miR482b/e均位...为进一步对大豆中的miR482家族有更全面的了解,本研究以大豆miR482家族为研究对象,对大豆中miR482家族的前体及成熟序列的特征、靶基因预测及其表达模式进行分析。在通过miRBase搜索得到的gma-miR482家族成熟序列中,除gma-miR482b/e均位于chr.20以外,其余序列均位于不同染色体。gma-miR482家族成熟序列核心区域具有较高的保守性,进一步分析共预测到24个gma-miR482的候选靶基因,其中大部分属于NBS-LRR家族(Nucleotide binding site leucine-rich repeats)。靶基因表达模式分析表明gma-miR482可能在大豆的不同发育时期发挥作用。本研究相关数据为深入解析大豆miR482家族的功能及分子调控机制提供了支持。展开更多
Verticillium wilt of potato is caused by the fungus pathogen Verticillium dahliae. Present sRNA sequencing data revealed that miR482 was in response to V. dahliae infection, but the function in potato is elusive. Here...Verticillium wilt of potato is caused by the fungus pathogen Verticillium dahliae. Present sRNA sequencing data revealed that miR482 was in response to V. dahliae infection, but the function in potato is elusive. Here, we characterized potato miR482 family and its putative role resistance to Verticillium wilt. Members of the potato miR482 superfamily are variable in sequence, but all variants target a class of disease-resistance proteins with nucleotide binding site （NBS） and leucine-rich repeat （LRR） motifs. When potato plantlets were infected with V. dahliae, the expression level of miR482e was downregulated, and that of several NBS-LRR targets of miR482e were upregulated. Transgenic potato plantlets overexpressing miR482e showed hypersensitivity to V. dahliae infection. Using sRNA and degradome datasets, we validated that miR482e targets mRNAs of NBS-LRR disease-resistance proteins and triggers the production of trans-acting （ta）- siRNAs, most of which target mRNAs of defense-relatedproteins. Thus, the hypersensitivity of transgenic potato could be explained by enhanced miR482e and miR482e-derived ta- siRNA-mediated silencing on NBS-LRR-disease-resistance pro- teins. It is speculated that a miR482-mediated silencing cascade mechanism is involved in regulating potato resistance against V. dahliae infection and could be a counter defense action of plant in response to pathogen infection.展开更多
文摘为进一步对大豆中的miR482家族有更全面的了解,本研究以大豆miR482家族为研究对象,对大豆中miR482家族的前体及成熟序列的特征、靶基因预测及其表达模式进行分析。在通过miRBase搜索得到的gma-miR482家族成熟序列中,除gma-miR482b/e均位于chr.20以外,其余序列均位于不同染色体。gma-miR482家族成熟序列核心区域具有较高的保守性,进一步分析共预测到24个gma-miR482的候选靶基因,其中大部分属于NBS-LRR家族(Nucleotide binding site leucine-rich repeats)。靶基因表达模式分析表明gma-miR482可能在大豆的不同发育时期发挥作用。本研究相关数据为深入解析大豆miR482家族的功能及分子调控机制提供了支持。
基金supported financially by grants from the National Natural Science Foundation of China (11171155)the National Pear Industry Technology System (CARS-29)a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions: Modern horticultural science (PAPD)
文摘Verticillium wilt of potato is caused by the fungus pathogen Verticillium dahliae. Present sRNA sequencing data revealed that miR482 was in response to V. dahliae infection, but the function in potato is elusive. Here, we characterized potato miR482 family and its putative role resistance to Verticillium wilt. Members of the potato miR482 superfamily are variable in sequence, but all variants target a class of disease-resistance proteins with nucleotide binding site （NBS） and leucine-rich repeat （LRR） motifs. When potato plantlets were infected with V. dahliae, the expression level of miR482e was downregulated, and that of several NBS-LRR targets of miR482e were upregulated. Transgenic potato plantlets overexpressing miR482e showed hypersensitivity to V. dahliae infection. Using sRNA and degradome datasets, we validated that miR482e targets mRNAs of NBS-LRR disease-resistance proteins and triggers the production of trans-acting （ta）- siRNAs, most of which target mRNAs of defense-relatedproteins. Thus, the hypersensitivity of transgenic potato could be explained by enhanced miR482e and miR482e-derived ta- siRNA-mediated silencing on NBS-LRR-disease-resistance pro- teins. It is speculated that a miR482-mediated silencing cascade mechanism is involved in regulating potato resistance against V. dahliae infection and could be a counter defense action of plant in response to pathogen infection.
