Application of traditional Chinese medicine acupoint needle embedding combined with emotional nursing in patients with gynecological malignant tumors

BACKGROUND Few relevant literature reports on applying acupoint press-needle embedding combined with emotional nursing in patients with a gynecological malignant tumor.AIM To explore the effect of traditional Chinese medicine acupoint needle embedding combined with emotional nursing on chemotherapy-related nausea and vomiting(CINV),cancer-related fatigue(CRF)and psychological state in patients with gynecological malignant tumors.METHODS Retrospective analysis of the clinical information of 84 patients with gynecological malignant tumors treated in our hospital from August 2020 to December 2022 Led to the development of an observation group(n=42)and a control group(n=42)based on various nursing approaches.Ondansetron hydrochloride injection was administered to the individuals in the control group.However,the observation group received emotional nursing based on the control group and acupoint pressneedle embedding of traditional Chinese medicine.Patients in both groups received the chemotherapy regimen of paclitaxel liposome+carbo-platin/cisplatin.For four weeks,both groups intervened.The CINV grade,quality of life,CRF,psychological status and sleep quality scores of the two groups before and RESULTS After intervention,the degree of CINV in the observation group was significantly better than that in the control group.After intervention,the scores of each dimension and total score of FLIE scale were significantly higher than those in the control group.After intervention,the scores of each dimension and total score of Piper Fatigue Scale were significantly lower than those in the control group(P<0.05).After intervention,the scores of avoidance and yield dimensions in the observation group were significantly lower than those in the control group,and the scores of confrontation dimension were significantly higher than those in the control group(P<0.05).After intervention,the sleep quality score of the observation group was significantly lower than that of the control group,and the Karnofsky Performance Status scale score was significantly higher than that of the control group(P<0.05).CONCLUSION The acupuncture point needle embedding of traditional Chinese medicine combined with emotional nursing can further reduce the incidence of chemotherapy-related nausea and vomiting in patients with gynecological malignant tumors,improve the quality of life and the degree of CRF,alleviate the bad psychological state,adopt a positive way to face the disease and treatment,and improve the quality of sleep and quality of life.

Investigation of the quality of life,mental status in patients with gynecological cancer and its influencing factors

BACKGROUND Having a gynecological tumor or undergoing treatment can be a traumatic experience for women,as it affects their self-image and sexual relationships and can lead to psychological reactions.Psychological adjustment following cancer occurrence remains a key issue among the survivors.AIM To examine the current status of quality of life(QoL),anxiety,and depression in patients with gynecological cancer and to analyze the factors associated with it.METHODS Data for 160 patients with gynecological malignancies treated at Shanxi Bethune Hospital from June 2020 to June 2023 were collected and analyzed retrospectively.Patients’QoL was assessed using the European Organization for Research on Treatment of Cancer Quality of Life Questionnaire Core 30 and the Functional Assessment of Cancer Therapy-General Questionnaire.Their emotional status was evaluated using the Self-Rating Anxiety/Depression Scale.The associated factors of anxiety and depression were analyzed.RESULTS The overall QoL score of the patients 6 months after surgery was 76.39±3.63 points.This included low levels of social and emotional function and severe fatigue and pain.The scores for physiological,functional,emotional,social,and family well-being exhibited an upward trend following surgery compared with those before surgery.One month after surgery,some patients experienced anxiety and depression,with an incidence of 18.75%and 18.13%,respectively.Logistic analysis revealed that good sleep was a protective factor against anxiety and depression in patients with gynecological tumors,whereas physical pain was a risk factor.CONCLUSION Patients with gynecological malignancies often experience anxiety and depression.By analyzing the factors that affect patients’QoL,effective nursing measures can be administered.

The Pre-Diagnosis History of Gynecologic Tumors in Children

Introduction: Gynecologic tumors represent less than 5% of all solid tumors in children. Patients and Method: Through a prospective and descriptive study over 4-years, we included girls aged less than 15 years admitted for a gynecologic tumor. We collected and analyzed the medical data, and paid particular attention to the pre-diagnosis history. Results: Eleven girls met our criteria with a mean age of 8.5 years (2 - 13 years). Abdominal pain, abdominal distension or protrusion of a vaginal mass was the main symptoms. The patients were seen at least by one doctor outside the surgical structure [1] [2] [3]. Four of eleven were received by two different doctors before carrying out the evocative radiological assessment. The average consultation delay was 7-months (24 hours to 18 months). There were nine ovarian tumors and two vaginal tumors. The histological study revealed five of eleven (45%) malignant tumors. Conclusion: Gynecological tumors in children are rare. However, there is a significant proportion of malignant tumors. All doctors can be confronted with it and should not delay the radiological assessment.

Gynecological tumors in patients with Peutz-Jeghers syndrome (PJS)

Peutz-Jeghers syndrome (PJS) is an autosomal dominant disorder characterized by the development of hamartomatous polyposis in the gastrointestinal tract and melanin-pigmented macules on the skin mucosa. The responsible gene is a tumor suppressor, STK11/LKB1, on chromosome 19p13.3. PJS complicates with benign and malignant tumors in various organs. In gynecology, there has been a particular focus on complications of PJS with sex cord tumor with annular tubules (SCTAT) and minimal deviation adenocarcinoma (MDA), which are rare diseases. Approximately 36% of patients with SCTAT are complicated with PJS and these patients are characterized by multifocal, bilateral, small and benign lesions that develop into tumors with mucinous to serous ratios of 8:1. In addition, 10% of cases of MDA are complicated with PJS and mutation of STK11, the gene responsible for PJS, has a major effect on onset and prognosis. The disease concept of lobular endocervical glandular hyper-plasia (LEGH) has recently been proposed and LEGH is thought to be a potential premalignant lesion of MDA, however, the relationship between PJS and LEGH remains unclear. Several case reports of PJS patients complicated with gynecological tumors have been published and further studies are needed to determine the underlying

Psychological Intervention and Nursing Analysis of Gynecological Malignant Tumors during Chemotherapy

Objectives:To explore the clinical effect of psychological intervention and nursing during the chemotherapy of gynecological malignant tumor.Methods:120 patients with gynecologic malignancies were selected as subjects.According to the nursing method,these patients were divided into intervention group and control group,with 60 cases in each group.The patients in the control group were given routine care,and the patients in the observation group were given psychological intervention care on the basis of routine nursing.Before and after treatment,the anxiety and depression of the two groups were compared using the Self-rating Anxiety Scale(SAS)and the Self-rating Depression Scale(SDS).The satisfaction and adverse reactions of the two groups were compared.The results were statistically analyzed.Results:After nursing intervention,the anxiety and depression scores of the intervention group were lower than those of the control group(P<0.05).The incidence of nausea,vomiting and fatigue in the intervention group was significantly lower than that in the control group(P<0.01).There was no significant difference in the symptoms of diarrhea between the two groups(P>0.05).After the treatment,the satisfaction of the intervention group was significantly higher than that of the control group(P<0.05).Conclusions:During the chemotherapy of gynecological malignant tumor patients,psychological intervention nursing can alleviate the anxiety and depression of patients,improve the complications,and improve the satisfaction of patients.It is worthy of clinical application.

miRNAs对食管癌发生发展的影响及中医药的干预作用

食管癌是常见的上消化道恶性肿瘤,在我国食管癌的总患病数和死亡数占全球的一半,极大地加重了我国癌症防治及医疗保障体系的负担。食管癌早期发病隐匿、不易察觉,导致多数患者在确诊时已处于中晚期,不利于治疗和预后。微小RNA(miRNAs)作为新型生物标志物,在肿瘤的诊断、病情监测和预后评估中发挥着重要作用。近年研究表明,miRNAs在食管癌微环境的形成,放化疗抵抗,肿瘤细胞增殖、凋亡、侵袭、自噬等过程中扮演着抑癌基因和抗癌基因的双重角色,是食管癌潜在的干预靶点,具有较高的研究价值和意义。但miRNAs在食管癌进展中的作用机制尚不明确,限制其作为肿瘤标志物应用于食管癌的临床诊断、治疗和预后。基于此,文章就miRNAs在食管癌发生发展中的双重调控机制及中医药干预研究进行系统综述,旨在明确miRNAs如何影响食管癌的发生与发展及潜在治疗策略,以期为食管癌的临床防治提供新思路。

Advances of Prevention and Nursing of Deep Venous Thrombosis after Gynecological Tumor Operation

Emainly includes deep venous thrombosis(DVT)and pulmonary thromboembolism(PTE).DVT is caused by the thrombogenesis of red blood cells,platelets and fibrous protein which obstructs the venous backflow leading to inflam-matory changes in the wall of the vein.PTE is caused by the caducous blood clots of DVT that runs with the blood to the lungs.Because DVT and PTE are the venous thromboembolic disease process in two stages,the prevention of DVT and PTE is very important for preventing the rapid onset of PTE and high mortality rate of the postoperative complications.The changes of female hormones and the blood concentration and lipid metabolism disorders make venous thromboembolism more likely to occur during pregnancy.Once the pulmonary vessels were blocked,the patients’life would be threatened severely.To find a more effective way to prevent postoperative venous thrombosis in gynecology we review the prevention and treatment of deep venous thrombosis after gynecological surgery in this paper.

B7同源物4分子对乳腺癌细胞miRNA表达谱的影响

目的通过解析免疫调控分子B7同源物4(B7-H4)对乳腺癌miRNA表达谱的影响,探讨B7-H4介导肿瘤免疫逃逸和乳腺癌进展的潜在机制.方法采用miRNA测序分析B7-H4敲除或过表达的乳腺癌细胞系,筛选共同的差异miRNA;通过qRT-PCR验证差异miRNA,采用TargetFinder和TargetScan预测差异miRNA的靶基因,同时进行基因本体(GO)和京都基因与基因组百科全书数据库(KEGG)信号通路的富集;采用Kaplan-Meier plotter对关键miRNA及靶基因进行生存分析.结果B7-H4敲除后有415个miRNA发生显著变化,而过表达后有134个差异miRNA,通过叠加筛选到36个共同的差异miRNA,通过qRT-PCR验证筛选获得14个差异miRNA;对其进行下游靶基因预测,得到TP53AIP1、RAD52、CDH7、FOXA1、CDH2和ZEB1等涉及细胞增殖和转移功能的基因;富集分析发现,靶基因主要参与癌症进展相关的MAPK和Ras信号通路等,推测其可能参与乳腺癌的病理进展.结论B7-H4通过参与乳腺癌miRNA的表观调控,影响关键靶基因的表达,介导细胞增殖和转移,影响乳腺癌的进展.

外泌体miRNA参与肿瘤化疗耐药的机制

背景:化疗作为癌症治疗的常见手段,前期具有一定的治疗效果,但随着化疗次数增加,肿瘤细胞对药物的抵抗性也逐渐增加,即化疗耐药性。目前化疗耐药性产生的主要机制包括化疗药物未能达到肿瘤部位,药物活性丧失导致药效下降,肿瘤细胞通过重塑代谢功能、基因表型和微环境逃避药物攻击等,但具体机制还有待深入探讨。目的:归纳并总结不同肿瘤中外泌体miRNA参与耐药的机制,提出潜在靶点。方法:以“hematological malignancies,liver cancer,lung cancer,breast cancer,melanoma,colorectal cancer,drug resistance,exosomal miRNAs”为英文检索词,检索PubMed数据库。检索时间范围为2013年1月至2023年8月。通过阅读文题和摘要进行初步筛选,排除与此次研究内容相关性差或内容重复的文献,最后纳入60篇文献进行综述。结果与结论:(1)外泌体miRNA进入细胞间质和循环,通过发挥局部旁分泌或远端效应,参与不同肿瘤生物进程的形成和发展,包括肿瘤微环境重塑、血管生成和耐药性产生;(2)外泌体miRNA可以透过细胞膜进入另一个细胞的胞浆内,通过传递miRNA启动或抑制受体细胞中的信号通路,在化疗过程中维持细胞存活、增殖和转移能力,从而导致耐药产生;(3)目前已经鉴定出许多与化疗耐药有关的外泌体miRNA,并将其作为常见肿瘤治疗靶点来开展研究;(4)虽然外泌体miRNA作为潜在的生物标志物和治疗靶点具有很大的前景,但仍有一些研究缺陷需要解决:一是缺乏用于外泌体分离和表征的标准化方案,导致不同研究中使用的方法存在差异;二是虽然外泌体中miRNA的存在和丰度已被广泛研究,但其功能作用和作用机制尚不完全清楚,阐明外泌体miRNA调控的特定靶点和途径可以为它们的生物学意义和潜在的治疗应用提供有价值的见解。解决这些研究缺陷将有助于更全面了解外泌体miRNA及其在各个领域的潜在应用,包括诊断、治疗和个性化医疗。

唾液腺腺样囊性癌微环境中肿瘤相关巨噬细胞外泌体差异表达miRNAs的筛选及验证

目的:探讨唾液腺腺样囊性癌(salivary adenoid cystic carcinoma,SACC)微环境中肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)外泌体miRNAs表达特征,分析其在SACC进展中的作用。方法:将SACC细胞与巨噬细胞共培养,获得SACC相关TAMs。应用超速离心法分别提取以上细胞外泌体,采用透射电镜、Western免疫印迹、外泌体纳米微粒追踪检测进行验证。对TAMs外泌体与对照组巨噬细胞外泌体进行RNA-seq测序,分析差异表达的miRNAs。利用miRanda和RNAhybrid软件对差异miRNAs进行靶基因预测,再对差异miRNAs作用的靶基因的集合分别进行GO和KEGG富集分析。利用qRT-PCR、CCK-8、细胞划痕实验、Transwell实验验证TAMs外泌体miRNAs表达及功能。采用SPSS 22.0软件包对数据进行统计学分析。结果:筛选出1595个差异表达的miRNAs,其中15个miRNAs表达差异变化具有统计学意义(P<0.05)。差异miRNAs作用靶基因富集分析发现,TAMs外泌体miRNAs靶基因参与调控癌症相关信号通路。qRT-PCR结果显示,TAMs外泌体has-miR-21-5p表达上调最显著。CCK-8、细胞划痕实验和Transwell实验发现,TAMs外泌体hsa-miR-21-5p促进SACC细胞增殖、迁移与侵袭能力。结论:TAMs外泌体hsa-miR-21-5p促进SACC细胞恶性进展。TAMs外泌体miRNAs可能在SACC进展中发挥重要作用,有望为SACC的诊治提供新的策略。

血清miRNA-183与CA199联合检测在胃癌诊断和预后评估中的价值

目的:探讨血清miRNA-183与糖类抗原199(CA199)联合检测在胃癌诊断和预后评估中的价值。方法:选取2017年1月-2021年12月在安徽医科大学附属滁州医院确诊的52例胃癌患者,将其病例资料纳入胃癌组,另选同期诊断为胃部良性病变的40例患者纳入对照组。采集其外周静脉血,分别采用逆转录实时荧光定量PCR(RT-qPCR)和化学发光免疫法检测血清miRNA-183、CA199水平,比较胃癌患者与对照组间的差异,绘制受试者工作特征曲线(ROC),分析以上指标单独及联合检测在胃癌诊断中的价值。分析血清miRNA-183和CA199水平与胃癌患者临床病理指标的关系,采用Kaplan-Meier生存曲线分析血清miRNA-183和CA199水平对胃癌患者预后的影响。结果:与对照组相比,胃癌组患者血清中miRNA-183表达水平明显升高,差异具有统计学意义(P<0.05)。组织病理分型为低-未分化、T3~T4以及TNM分期为Ⅲ~Ⅳ期胃癌患者血清miRNA-183的表达水平较组织病理分型为中-高分化、T1~T2和TNM分期为Ⅰ~Ⅱ期的胃癌患者明显升高(P<0.05);组织病理分型为低-未分化、TNM分期为Ⅲ-Ⅳ期胃癌患者血清CA199的表达水平较组织病理分型为中-高分化、TNM分期为Ⅰ-Ⅱ期的胃癌患者亦明显升高(P<0.05)。采用ROC曲线分析得出miRNA-183相对表达量4.11为诊断胃癌的最佳临界值,灵敏度与特异度分别为67.31%和80%,ROC曲线下面积(AUC)为0.771;CA199以46.17 U/mL为诊断胃癌的最佳临界值,敏感度与特异度分别为53.8%和87.5%,AUC为0.724;联合检测血清miRNA-183与CA199诊断胃癌对应的敏感度及特异度分别为76.92%和87.5%,AUC为0.882。miRNA-183联合CA199诊断胃癌的AUC大于miRNA-183或CA199独立诊断,差异均具有统计学意义(P<0.05)。Kaplan-Meier分析显示,miRNA-183高表达水平组患者的预后较miRNA-183低水平组患者差,miRNA-183和CA199均高表达患者预后更差(P<0.05)。结论:胃癌患者血清miRNA-183表达明显升高,且与肿瘤的T分期、TNM分期、组织学分级相关;血清miRNA-183与CA199联合检测可以提高胃癌诊断效能,预测胃癌患者的预后。

Innovative insights into extrachromosomal circular DNAs in gynecologic tumors and reproduction

Originating but free from chromosomal DNA,extrachromosomal circular DNAs(eccDNAs)are organized in circular form and have long been found in unicellular and multicellular eukaryotes.Their biogenesis and function are poorly understood as they are characterized by sequence homology with linear DNA,for which few detection methods are available.Recent advances in high-throughput sequencing technologies have revealed that eccDNAs play crucial roles in tumor formation,evolution,and drug resistance as well as aging,genomic diversity,and other biological processes,bringing it back to the research hotspot.Several mechanisms of eccDNA formation have been proposed,including the breakage-fusion-bridge(BFB)and translocation-deletion-amplification models.Gynecologic tumors and disorders of embryonic and fetal development are major threats to human reproductive health.The roles of eccDNAs in these pathological processes have been partially elucidated since the first discovery of eccDNA in pig sperm and the double minutes in ovarian cancer ascites.The present review summarized the research history,biogenesis,and currently available detection and analytical methods for eccDNAs and clarified their functions in gynecologic tumors and reproduction.We also proposed the application of eccDNAs as drug targets and liquid biopsy markers for prenatal diagnosis and the early detection,prognosis,and treatment of gynecologic tumors.This review lays theoretical foundations for future investigations into the complex regulatory networks of eccDNAs in vital physiological and pathological processes.

miRNA与放射性肺损伤相关性研究进展

miRNA几乎在所有的真核生物中均有表达,在许多生物学进程和疾病发展中扮演着不同的角色,最近它与放射性肺损伤(RILI)之间的联系也正逐渐被揭开。作为放疗最常见的并发症之一,RILI目前的治疗措施仍非常有限。本文对miRNA调节RILI的潜在机制进行综述,介绍了miRNA在基因组水平对RILI的保护,阐述了miRNA通过调节细胞因子的分泌和核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)炎症小体的功能减轻放射性肺炎以及抑制转化生长因子-β(TGF-β)/Smad信号通路和上皮细胞-间充质转化延缓放射性肺纤维化的进程。

Roles of endoplasmic reticulum stress and apoptosis signaling pathways in gynecologic tumor cells：A systematic review

Efficient functioning of the endoplasmic reticulum(ER) is very important for most cellular activities, such as protein folding and modification. The ER closely interacts with other organelles, including the Golgi body, endosome, membrane, and mitochondria, providing lipids and proteins for the repair of these organelles. ER stress can be induced by various abnormal materials in the cell. ER stress is a compensatory intracellular environment disorder that occurs during areaction. ER can sense the stress and respond to it through translational attenuation, upregulation of the genes for ER chaperones and related proteins, and degradation of unfolded proteins by a quality-control system, but excessive ER activation can cause cell death. The Pubmed and Web of Science databases were searched for full-text articles, and the terms "endoplasmic reticulum stress/unfolded protein response/gynecologic tumor cell apoptosis" were used as key words. Thirty-five studies of ER stress and unfolded protein response published from 2000 to 2016 were analyzed. Stress triggers apoptosis through a variety of signaling pathways. Increasing evidence has shown that the ER plays an important role in tumor cell diseases. The present review discusses the molecular mechanisms underlying unfolded protein response and its ability to promote survival and proliferation in gynecologic tumor cells.

Clinical analysis of abdominal aorta block in operation of gynecologic tumor

Objective:To evaluate the clinical effects of the abdominal aorta block in controlling haemorrhage during operations of the gynecologic tumor. Methods: From July 1965 to January 2005. we collected patients (n = 49) of gynecologic tumor complicated with haemorrhage during operations, who were divided into 3 groups: preventive blocking group (PG, n=12), treatment blocking group (TG, n = 20) used abdominal aorta block technique with sterilized cotton band and silica gel tube, and control group (CG, n = 17) which were used the regular haemostatic methods, such as ligature, suture and ribbon gauze packing. During operations, the vital signs including the amount of bleeding and transfusion were measured. Results: Compared with the CG, the amount of bleeding and transfusion in the PG and TG decreased significantly (P<0. 01). After using the technique, 32 cases of haemorrhage were controlled completely. All patients finished operation smoothly in the end and the vital signs were stable. The vision field of operation was clear and the operating time was shortened dramatically (3. 0 h vs 5. 7 h and 3. 8 h vs 5. 7 h, P< 0. 01). No complications caused by the block occurred in the post-operation. Conclusion: Lower abdominal aorta block is safe and effective in controlling haemorrhage during operations of the gynecologic tumor.

探究CUL4B在结直肠癌患者来源的肿瘤类器官中调控miRNAs

目的探究CUL4B在肿瘤患者来源的结直肠癌肿瘤类器官中调控miRNAs。方法利用干扰CUL4B的和其对照的PDOs样本,进行miRNA-seq,对差异miRNAs的靶基因进行KEGG和GO等分析。利用TargetScan和miRDB 2个数据库分别预测可能受CUL4B调控的并且评分≥85分的miRNAs,结合miRNA-seq差异miRNAs进行韦恩分析。结果miRNA-seq结果显示共有41个差异miRNAs。TargetScan和miRDB两个数据库和测序数据韦恩分析发现miR495-3p、miR381-3p和miR148b-5p 3个共同交集的miRNAs。KEGG结果显示代谢通路富集的基因个数最多,溶酶体富集基因Q值最小。GO分析结果显示单有机体过程、蛋白质结合和细胞质富集基因最多。流式细胞仪检测发现干扰CUL4B的表达并不影响PDOs的细胞周期。结论CUL4B可能是通过代谢通路和溶酶体2个信号通路调控miR495-3p、miR381-3p和miR148b-5p。

Research Progress on the Role of miRNA-448 in Tumor

MicroRNA (miRNA) is a class of endogenous non-coding and regulatory single stranded small RNA molecules, about 18 - 24 nucleotides in length. More than 800 miRNA coding genes have been identified in the human genome, and about 113 target genes are predicted to be regulated, which distinguishes it from most oligonucleotides and functional RNA degraded fragments. It is involved in the regulation of cell proliferation, differentiation, apoptosis and other cell activities. At the same time, it is abnormal in liver cancer, breast cancer, glioma and other tumors, becoming a new biomarker, and participating in cancer differentiation, invasion and metastasis through the interaction with multiple target genes, such as SATB1, NF-KB and 5-HT2B. Mir-448 is abnormally expressed in a variety of tumor cells, such as liver cancer and non-small cell lung cancer. mirNA-448 may play a very important role in tumors. In order to deepen the understanding of the role of mirNA-448 in tumors, this paper reviews the research progress of the role of mirNA-448 in tumors.

基于miRNA-132信号通路探究胡椒碱对肝癌血管形成及肿瘤抑制作用

目的:基于miRNA-132信号通路探究胡椒碱对肝癌血管形成及肿瘤抑制作用。方法:制备肝癌细胞转移瘤,将裸鼠分为空白组(不制备肝癌裸鼠模型)、模型组(肝癌小鼠模型组)、胡椒碱组(肝癌小鼠模型给予胡椒碱干预组)、miR-132组(肝癌小鼠模型给予miR-132类似物组)、联合组(肝癌小鼠模型给予胡椒碱联合miR-132类似物组)。比较各组裸鼠的瘤体积和肿瘤抑制率;比较肿瘤组织病理学变化;用ELISA检测血清中血管内皮生长因子(VEGF)含量;比较微血管密度(MVD)数量;用蛋白质印迹法检测肝组织中miR-132、GP73表达。结果:和模型组相比,miR-132组、胡椒碱组和联合组裸鼠的瘤体积均显著降低,且联合组瘤体积较胡椒碱组和miR-132组降低更明显(P<0.05)。和模型组相比,miR-132组、胡椒碱组和联合组裸鼠肿瘤生长抑制率显著增加(P<0.05);联合组裸鼠的肿瘤生长抑制率明显高于miR-132组和胡椒碱组(P<0.05)。模型组裸鼠血清中VEGF含量和MVD数量和空白组相比显著增加(P<0.05);和模型组相比,miR-132组、胡椒碱组和联合组裸鼠血清中VEGF含量和MVD数量均显著减少,且联合组血清中VEGF含量和MVD显著低于胡椒碱组(P<0.05)。和空白组相比,模型组裸鼠肝组织中miR-132表达显著降低,GP73蛋白表达显著增加(P<0.05);和模型组相比,miR-132组、胡椒碱组和联合组裸鼠肝组织中miR-132表达均明显增加,GP73蛋白表达均显著降低,且联合组中miR-132和GP73表达变化最大(P<0.05)。双荧光素酶检测报告结果显示,过表达miR-132可降低GP73活性(P<0.05)。结论:胡椒碱可通过调控miR-132负向调控GP73抑制肝癌血管形成,同时也能显著抑制肿瘤的生长。

miRNA-15a/16调控Bmi-1蛋白在卵巢癌顺铂化疗耐药中的作用

目的探讨miRNA-15a和miRNA-16在逆转卵巢癌顺铂化疗耐药过程中的作用机制。方法使用miRNA-15a和miRNA-16模拟物转染人卵巢癌顺铂耐药细胞株CoC1/DDP,予顺铂处理,qRT-PCR检测正常培养CoC1/DDP细胞组、顺铂处理组、阴性对照组、转染miRNA-15a组、转染miRNA-16组和过表达的Bmi-1质粒的转染miRNA-15a和miRNA-16组的miRNA-15a、miRNA-16的表达水平,同时应用Western blot检测各组细胞中Bmi-1的表达水平,CCK-8法、Annexin V/PI法检测每组细胞的存活及凋亡情况、γ-H2AX免疫荧光检测细胞凋亡情况。结果卵巢癌顺铂耐药细胞株CoC1/DDP中miRNA-15a和miRNA-16低表达,Bmi-1蛋白高表达。过表达miRNA-15a、miRNA-16水平,Bmi-1蛋白下降(P<0.05),细胞存活率下降(P<0.05),DNA凋亡明显增加(P<0.05),DNA损伤程度更严重(P<0.05)。过表达Bmi-1质粒可提高细胞活度(P<0.05),降低细胞凋亡率(P<0.05)。结论Bmi-1蛋白可能是miRNA-15a和miRNA-16的调节蛋白靶点,高表达miRNA-15a和miRNA-16可以通过降低Bmi-1蛋白来提高顺铂对卵巢癌细胞的敏感性。通过对卵巢癌顺铂化疗耐药性分子标记的预测和对卵巢癌肿耐药性目标的攻克提供了新的思路。

肿瘤教化血小板转录组中miRNA区分肺结节良、恶性的可行性研究进展

肺癌是世界上致死率最高的恶性肿瘤之一,液体活检是目前肿瘤研究的热点,已有部分应用于临床,具有早期筛查、定期检测疗效、预测预后等优势。其中肿瘤教化血小板作为新型液体活检标志物来源,是目前用于检测早期NSLCL的热门生物标志物,可通过分析肿瘤教化血小板的转录组可以区分健康人与肺癌患者。而目前发现部分miRNA在肺结节的良恶性鉴别方面有着重要意义。本文就肿瘤教化血小板转录组中miRNA区分肺结节良、恶性的可行性研究进展进行综述。