目的探讨miRNA155-5p在异基因造血干细胞移植后口腔慢性移植物抗宿主病(chronic graft versus host disease,cGVHD)患者血浆中的表达水平及意义。方法选取异基因造血干细胞移植后确诊为口腔cGVHD的患者18人纳入口腔cGVHD组,异基因造血...目的探讨miRNA155-5p在异基因造血干细胞移植后口腔慢性移植物抗宿主病(chronic graft versus host disease,cGVHD)患者血浆中的表达水平及意义。方法选取异基因造血干细胞移植后确诊为口腔cGVHD的患者18人纳入口腔cGVHD组,异基因造血干细胞移植后没有出现口腔cGVHD的患者18人为无口腔cGVHD组,对照组纳入健康者18人。收集三组血液,检测血浆中miRNA155-5p的表达水平并对比分析。结果口腔cGVHD组患者血浆中miRNA155-5p水平低于健康对照组;差异有统计学意义(P<0.05)。结论血浆中miRNA155-5p表达异常可能参与口腔c GVHD的发生。展开更多
In the state of acute myocardial ischemia,miRNA expression can regulate related genes and proteins,reduce myocardial cell damage,and thus play a protective role in the myocardium.However,the specific mechanism still n...In the state of acute myocardial ischemia,miRNA expression can regulate related genes and proteins,reduce myocardial cell damage,and thus play a protective role in the myocardium.However,the specific mechanism still needs to be further explored.Recent studies have found that the opening of the mitoKATP channel can regulate mitochondrial autophagy,and the initiation of miRNA-DNA methylation plays a regulatory role in inducing cell autophagy.The applicant research team previously found that Qishen Yiqi Dropping Pills could significantly improve myocardial ischemia by mediating MitokATP channels to regulate mitochondrial autophagy.,and animal experiments have confirmed that miR-155 plays a significant role in the aspect of autophagy regulates,inflammatory reaction and Vascular smooth muscle cell migration.Therefore,the applicant innovatively proposed that Qishen Yiqi Dropping Pills can regulate miRNA155-DNA methylation to mediate the opening of mitoKATP,thereby regulating mitochondrial autophagy and improving myocardial ischemia.In this paper,the association between mitochondrial autophagy and oxidative stress injury after myocardial ischemia was described,and the possible mechanism of Qisen Yiqi dropping pills regulating mitochondrial autophagy by regulating miRNA155-DNA methylation to mediate MitokATP to improve myocardial ischemia reperfusion injury was discussed,so as to provide theoretical ideas for related research.展开更多
目的:分析miRNA155、癌胚抗原(Carcinoembryonic antigen,CEA)、神经元特异性烯醇酶(Neuron specific enolase,NSE)联合检测在早期诊断肺癌的价值。方法:将我院2018年4月至2021年1月期间收治的52例早期肺癌作为病例组,同期接受检查的40...目的:分析miRNA155、癌胚抗原(Carcinoembryonic antigen,CEA)、神经元特异性烯醇酶(Neuron specific enolase,NSE)联合检测在早期诊断肺癌的价值。方法:将我院2018年4月至2021年1月期间收治的52例早期肺癌作为病例组,同期接受检查的40例肺部良性病变者做为病变组,同期接受健康体检的42例作为健康组。三组均接受miRNA155、CEA以及NSE检查。对比上述三者单独检测以及联合检测对早期肺癌的诊断价值。结果:病例组miRNA155、CEA以及NSE水平均高于病变组和健康组(P<0.05);病例组miRNA155、CEA以及NSE阳性率均高于病变组和健康组(P<0.05);miRNA155、CEA以及NSE诊断符合率、敏感度、特异度均低于联合检测(P<0.05)。结论:miRNA155、CEA、NSE在早期肺癌中均有表达,且联合检测对早期肺癌诊断价值更高。展开更多
目的检测湿疹患儿1,25-二羟维生素D3(1,25-dihydroxyvitamin D3,1,25(OH)2D3)、维生素D受体(vitamin D receptor,VDR)、微小核糖核酸155(micro RNA155,miRNA155)、细胞因子信号抑制因子一1(Suppressor of cytokine signaling-1,SOCS1)...目的检测湿疹患儿1,25-二羟维生素D3(1,25-dihydroxyvitamin D3,1,25(OH)2D3)、维生素D受体(vitamin D receptor,VDR)、微小核糖核酸155(micro RNA155,miRNA155)、细胞因子信号抑制因子一1(Suppressor of cytokine signaling-1,SOCS1)及淋巴细胞亚群的量,并探讨其相互关系。方法采用电化学发光法检测受试者外周血1,25(OH)2D3的表达,流式细胞术检测患者T细胞百分比及CD3、CD4、CD8阳性细胞含量,采用RT-PCR方法检测皮损处VDR、miRNA155、SOCS1。结果湿疹患儿1,25(OH)2D3及VDR(29.25±11.03 nmol/L,1.74±0.92)明显低于健康儿童(55.49±21.66 nmol/L,3.62±1.68);湿疹患儿T细胞亚群68.65±16.74%及CD4+的T淋巴细胞亚群38.83±10.82%明显高于对照组(64.47±17.23,33.25±11.95%),CD8+的T淋巴细胞亚群25.68±6.81%明显低于对照组29.01±7.93%;湿疹患者皮损处VDR、SOCS的表达(1.74±0.92,1.78±1.02)明显低于对照组(3.62±1.68,3.13±1.70),miRNA1551的表达5.41±3.23明显高于对照组2.06±0.84。结论湿疹患儿1,25(OH)2D3、VDR表达异常,且其可调节湿疹患儿miR155/SOCS1通路。展开更多
目的探讨系统性红斑狼疮(SLE)女性患者外周血中miR-155及其靶基因C/EBPβ的表达水平与滤泡辅助性T细胞(follicular helper T cell,Tfh)和滤泡调节性T细胞(follicular T regulatory cell,Tfr)频率的相关性。方法选择陆军军医大学第一附...目的探讨系统性红斑狼疮(SLE)女性患者外周血中miR-155及其靶基因C/EBPβ的表达水平与滤泡辅助性T细胞(follicular helper T cell,Tfh)和滤泡调节性T细胞(follicular T regulatory cell,Tfr)频率的相关性。方法选择陆军军医大学第一附属医院收治的29例SLE女性患者,以同期来院体检的20名女性健康人作为对照,利用流式细胞仪检测外周血Tfh和Tfr细胞的频率,并统计Tfh/Tfr比值,利用实时定量聚合酶链反应(qPCR)和免疫印迹法分别检测miR-155和C/EBPβ的表达,同时分析以上检测指标与SLE疾病活性指数(SLEDAI)的关联性,以及miR-155和C/EBPβ的表达水平与Tfh和Tfr细胞频率和Tfh/Tfr比值的相关性。结果SLE女性患者外周血CD4^(+)T细胞中Tfh和Tfr细胞频率均与SLEDAI呈正相关,且与Tfh/Tfr比值呈负相关趋势,但无显著性差异;miR-155和C/EBPβ在活动期患者中的表达显著高于缓解期和对照组,且与SLEDAI正相关;患者中miR-155和C/EBPβ的表达水平均与Tfh细胞频率显著正相关。结论SLE女性患者外周血miR-155和C/EBPβ的表达水平同SLEDAI及Tfh细胞频率正相关,提示miR-155和C/EBPβ可能通过调控Tfh细胞的分化与功能而参与SLE疾病的发生与发展。展开更多
基金Regional Fund Project of National Natural Science Foundation of China(No.81460712)the Young and Middle-aged Teachers’Scientific Research Basic Ability Enhancement Project of Guangxi Universities(No.2020KY07026)Guangxi Graduate Education Innovation Program(No.YCXJ2021035)。
文摘In the state of acute myocardial ischemia,miRNA expression can regulate related genes and proteins,reduce myocardial cell damage,and thus play a protective role in the myocardium.However,the specific mechanism still needs to be further explored.Recent studies have found that the opening of the mitoKATP channel can regulate mitochondrial autophagy,and the initiation of miRNA-DNA methylation plays a regulatory role in inducing cell autophagy.The applicant research team previously found that Qishen Yiqi Dropping Pills could significantly improve myocardial ischemia by mediating MitokATP channels to regulate mitochondrial autophagy.,and animal experiments have confirmed that miR-155 plays a significant role in the aspect of autophagy regulates,inflammatory reaction and Vascular smooth muscle cell migration.Therefore,the applicant innovatively proposed that Qishen Yiqi Dropping Pills can regulate miRNA155-DNA methylation to mediate the opening of mitoKATP,thereby regulating mitochondrial autophagy and improving myocardial ischemia.In this paper,the association between mitochondrial autophagy and oxidative stress injury after myocardial ischemia was described,and the possible mechanism of Qisen Yiqi dropping pills regulating mitochondrial autophagy by regulating miRNA155-DNA methylation to mediate MitokATP to improve myocardial ischemia reperfusion injury was discussed,so as to provide theoretical ideas for related research.
文摘目的:分析miRNA155、癌胚抗原(Carcinoembryonic antigen,CEA)、神经元特异性烯醇酶(Neuron specific enolase,NSE)联合检测在早期诊断肺癌的价值。方法:将我院2018年4月至2021年1月期间收治的52例早期肺癌作为病例组,同期接受检查的40例肺部良性病变者做为病变组,同期接受健康体检的42例作为健康组。三组均接受miRNA155、CEA以及NSE检查。对比上述三者单独检测以及联合检测对早期肺癌的诊断价值。结果:病例组miRNA155、CEA以及NSE水平均高于病变组和健康组(P<0.05);病例组miRNA155、CEA以及NSE阳性率均高于病变组和健康组(P<0.05);miRNA155、CEA以及NSE诊断符合率、敏感度、特异度均低于联合检测(P<0.05)。结论:miRNA155、CEA、NSE在早期肺癌中均有表达,且联合检测对早期肺癌诊断价值更高。
文摘目的检测湿疹患儿1,25-二羟维生素D3(1,25-dihydroxyvitamin D3,1,25(OH)2D3)、维生素D受体(vitamin D receptor,VDR)、微小核糖核酸155(micro RNA155,miRNA155)、细胞因子信号抑制因子一1(Suppressor of cytokine signaling-1,SOCS1)及淋巴细胞亚群的量,并探讨其相互关系。方法采用电化学发光法检测受试者外周血1,25(OH)2D3的表达,流式细胞术检测患者T细胞百分比及CD3、CD4、CD8阳性细胞含量,采用RT-PCR方法检测皮损处VDR、miRNA155、SOCS1。结果湿疹患儿1,25(OH)2D3及VDR(29.25±11.03 nmol/L,1.74±0.92)明显低于健康儿童(55.49±21.66 nmol/L,3.62±1.68);湿疹患儿T细胞亚群68.65±16.74%及CD4+的T淋巴细胞亚群38.83±10.82%明显高于对照组(64.47±17.23,33.25±11.95%),CD8+的T淋巴细胞亚群25.68±6.81%明显低于对照组29.01±7.93%;湿疹患者皮损处VDR、SOCS的表达(1.74±0.92,1.78±1.02)明显低于对照组(3.62±1.68,3.13±1.70),miRNA1551的表达5.41±3.23明显高于对照组2.06±0.84。结论湿疹患儿1,25(OH)2D3、VDR表达异常,且其可调节湿疹患儿miR155/SOCS1通路。
文摘目的探讨系统性红斑狼疮(SLE)女性患者外周血中miR-155及其靶基因C/EBPβ的表达水平与滤泡辅助性T细胞(follicular helper T cell,Tfh)和滤泡调节性T细胞(follicular T regulatory cell,Tfr)频率的相关性。方法选择陆军军医大学第一附属医院收治的29例SLE女性患者,以同期来院体检的20名女性健康人作为对照,利用流式细胞仪检测外周血Tfh和Tfr细胞的频率,并统计Tfh/Tfr比值,利用实时定量聚合酶链反应(qPCR)和免疫印迹法分别检测miR-155和C/EBPβ的表达,同时分析以上检测指标与SLE疾病活性指数(SLEDAI)的关联性,以及miR-155和C/EBPβ的表达水平与Tfh和Tfr细胞频率和Tfh/Tfr比值的相关性。结果SLE女性患者外周血CD4^(+)T细胞中Tfh和Tfr细胞频率均与SLEDAI呈正相关,且与Tfh/Tfr比值呈负相关趋势,但无显著性差异;miR-155和C/EBPβ在活动期患者中的表达显著高于缓解期和对照组,且与SLEDAI正相关;患者中miR-155和C/EBPβ的表达水平均与Tfh细胞频率显著正相关。结论SLE女性患者外周血miR-155和C/EBPβ的表达水平同SLEDAI及Tfh细胞频率正相关,提示miR-155和C/EBPβ可能通过调控Tfh细胞的分化与功能而参与SLE疾病的发生与发展。
