A simple,eco-friendly.sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-（β-hydroxyethyl）rutosidcs.The procedure was based on the use of an anionic surfactant ...A simple,eco-friendly.sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-（β-hydroxyethyl）rutosidcs.The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-（β-hydroxyethyl）rutosides fluorescence enhancement,increasing considerably the sensitivity of detection.All the variables affecting the fluorescence intensity were studied and optimized.The flow rate was 5 mL/min with detection at 450 nm（after excitation at 346 nm）.A linear correlation between drug amount and peak area was established for 0-（β-hydroxyethyl）rulosides in the range of 0.01-200 μg/mL with a detection limit of0.001 μg/mL（s/n = 3）.Validation processes were performed by recovering studies with satisfactory results.The new methodology can be employed for the routine analysis of 0-（P-hydroxyethyl）rutosides in bulks as well as in commercial formulations.展开更多
基金the National University of San Luis(Project 22/Q228)INQUISAL-CONICET(Instituto de Quimica de San Luis-Consejo Nacional de Investigaciones Cientificas y Tecnicas, PIP-CONICET 11220100100405) for the financial support
文摘A simple,eco-friendly.sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-（β-hydroxyethyl）rutosidcs.The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-（β-hydroxyethyl）rutosides fluorescence enhancement,increasing considerably the sensitivity of detection.All the variables affecting the fluorescence intensity were studied and optimized.The flow rate was 5 mL/min with detection at 450 nm（after excitation at 346 nm）.A linear correlation between drug amount and peak area was established for 0-（β-hydroxyethyl）rulosides in the range of 0.01-200 μg/mL with a detection limit of0.001 μg/mL（s/n = 3）.Validation processes were performed by recovering studies with satisfactory results.The new methodology can be employed for the routine analysis of 0-（P-hydroxyethyl）rutosides in bulks as well as in commercial formulations.
