A single molecule detection technique was developed by the combination of a single channel poly （dimethylsiloxane）/glass micro-fluidic chip and fluorescence correlation spectroscopy （FCS）. This method was successf...A single molecule detection technique was developed by the combination of a single channel poly （dimethylsiloxane）/glass micro-fluidic chip and fluorescence correlation spectroscopy （FCS）. This method was successfully used to determine the proportion of two model components in the mixture containing fluorescein and the rhodamine-green succinimidyl ester.展开更多
On the basis of the research on the status and problems of micro/nano bio-particles manipulation using dielectrophoresis, the theoretical basis and the model simulation of micro/nano bio-particles manipu-lation using ...On the basis of the research on the status and problems of micro/nano bio-particles manipulation using dielectrophoresis, the theoretical basis and the model simulation of micro/nano bio-particles manipu-lation using light-induced dielectrophoresis were discussed. The space distribution of electric field and dielectrophoresis forces in different heights were also obtained. On this basis, the core component of the micro manipulation system, that is, photoconductive layer of the chip, was completed in the mate-rial selection, fabricating process and performance analysis testing. Then the voltage drop of the sus-pension and the effective voltage frequency spectrum were obtained. Finally, by combining the machine vision detection with real-time tracking system, the micro/nano bio-particles manipulation platform based on the light-induced dielectrophoreisis was established, and then the manipulations for micro/nano bio-particles, such as quick collection, transport, separation, were implemented. This provided a basis for rapid, accurate, and low-cost detection of serious diseases based on the micro-fluidic biochip and early diagnosis.展开更多
A new structure and working principle of the chip integrated with micro valveless pump for capillary electrophoresis was proposed in this paper. The micro valveless pump with plane structure has advantages of simple s...A new structure and working principle of the chip integrated with micro valveless pump for capillary electrophoresis was proposed in this paper. The micro valveless pump with plane structure has advantages of simple structure, and the process technology is compatible with existing micro chips for capillary electrophoresis. Based upon the mathematical model, simulation study of micro pump was carried out to investigate the influence of structural parameters on flow characteristics, and the performance of the integrated micro pump was also tested with different control parameters. The simulation results agree with the experimental results. Three samples, which are amino acid, fluorescein and buffer solution, have been examined with this chip. The results of the primary experiments showed that the micro valveless pump was promising in the integration and automatization of miniature integrated fluidic systems.展开更多
利用原位聚合法在玻璃微管道内制备阴离子交换型固相萃取(SPE)微柱,以NO-2 为分析对象,针对NaNO2 KI Luminol发光体系设计微流控芯片,并将SPE微柱与微流控芯片连接起来组建成带有SPE微柱的复合式微流控芯片。分析了SPE微柱对NO-2 的吸...利用原位聚合法在玻璃微管道内制备阴离子交换型固相萃取(SPE)微柱,以NO-2 为分析对象,针对NaNO2 KI Luminol发光体系设计微流控芯片,并将SPE微柱与微流控芯片连接起来组建成带有SPE微柱的复合式微流控芯片。分析了SPE微柱对NO-2 的吸附保留与富集作用,在复合式微流控芯片上,实现了NO-2的进样、分离富集和检测,通过漏点曲线和交换容量两种方法分析了SPE微柱的柱容量。为控制SPE微柱的最大进样体积提供有利保障,并实现了食品中NO-2 的在线分离富集与检测。展开更多
基金This work was financially supported by the National Natural Science Foundation of China. （No.20271033, 20335020, 90408014）.
文摘A single molecule detection technique was developed by the combination of a single channel poly （dimethylsiloxane）/glass micro-fluidic chip and fluorescence correlation spectroscopy （FCS）. This method was successfully used to determine the proportion of two model components in the mixture containing fluorescein and the rhodamine-green succinimidyl ester.
基金Supported by the National High Technology Research and Development Program of China ("863" Program) (Grant No. 2006AA04Z351)the National Natural Science Foundation of China (Grant Nos. 50675033, 30770553)
文摘On the basis of the research on the status and problems of micro/nano bio-particles manipulation using dielectrophoresis, the theoretical basis and the model simulation of micro/nano bio-particles manipu-lation using light-induced dielectrophoresis were discussed. The space distribution of electric field and dielectrophoresis forces in different heights were also obtained. On this basis, the core component of the micro manipulation system, that is, photoconductive layer of the chip, was completed in the mate-rial selection, fabricating process and performance analysis testing. Then the voltage drop of the sus-pension and the effective voltage frequency spectrum were obtained. Finally, by combining the machine vision detection with real-time tracking system, the micro/nano bio-particles manipulation platform based on the light-induced dielectrophoreisis was established, and then the manipulations for micro/nano bio-particles, such as quick collection, transport, separation, were implemented. This provided a basis for rapid, accurate, and low-cost detection of serious diseases based on the micro-fluidic biochip and early diagnosis.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.50335010,50175098).
文摘A new structure and working principle of the chip integrated with micro valveless pump for capillary electrophoresis was proposed in this paper. The micro valveless pump with plane structure has advantages of simple structure, and the process technology is compatible with existing micro chips for capillary electrophoresis. Based upon the mathematical model, simulation study of micro pump was carried out to investigate the influence of structural parameters on flow characteristics, and the performance of the integrated micro pump was also tested with different control parameters. The simulation results agree with the experimental results. Three samples, which are amino acid, fluorescein and buffer solution, have been examined with this chip. The results of the primary experiments showed that the micro valveless pump was promising in the integration and automatization of miniature integrated fluidic systems.
