Osteoblast Behavior on Hierarchical Micro-/Nano-Structured Titanium Surface

In the present work, osteoblast behavior on a hierarchical micro-/nano-structured titanium surface was investigated. A hi- erarchical hybrid micro-/nano-structured titanium surface topography was produced via Electrolytic Etching （EE）. MG-63 cells were cultured on disks for 2 h to 7 days. The osteoblast response to the hierarchical hybrid micro-/nano-structured titanium surface was evaluated through the osteoblast cell morphology, attachment and proliferation. For comparison, MG-63 cells were also cultured on Sandblasted and Acid-etched （SEA） as well as Machined （M） surfaces respectively. The results show signifi- cant differences in the adhesion rates and proliferation levels of MG-63 cells on EE, SLA, and M surfaces. Both adhesion rate and proliferation level on EE surface are higher than those on SLA and M surfaces. Therefore, we may expect that, comparing with SLA and M surfaces, bone growth on EE surface could be accelerated and bone formation could be promoted at an early stage, which could be applied in the clinical practices for immediate and early-stage loadings.

Research on Nanofabrication Technology of Micro-/Nano-Stereo Rapid Prototyping of PCVD

At present, the most common micro/nano-scale fabri ca tion processes include the plane silicon process based on IC technology, stereo silicon process, LIGA, quasi-LIGA based on near ultra violet deep lithography, MEMS, energy beam etching and micro/nano-machining, etc. A common problem for t hese processes is the difficulty to fabricate arbitrary form for 3-dimensional micro/nano-parts, devices or mechanisms. To develop advanced MEMS manufacturin g technology, and to achieve fabrication of true 3-dimensional parts, devices or mechanisms, this paper proposes a nanofabrication technology for rapid proto typing of 3-dimensional parts, using plasma chemical vapor deposition (PCVD). This process can be describes as follows: A laser beam is produced by a low power, quasi molecule laser. It enters the vac uum chamber through a window, and is focused on with the substrate surface. A ga s in the chamber is ionized by the laser beam to produce PCVD on the substrate s urface, and forms a particle of the size of Ф100 nm (its thickness is about 100 nm). When the laser beam moves along X-axis, many particles form a line. Then the laser beam moves one step in Y-axis to form a new line. A plane is complete d by many lines. Then the substrate moves in Z-axis to form new plane. Eventu ally, many planes form a 3-dimensional component. Using available CAD/CAM softw are with this process, rapid prototyping of complex components can be achieved. A nanometer precision linear motor, such as that described in Chinese national p atent (patent No. ZL 98 2 16753.9), can be used to obtain the nanometer precisio n movements in the process. The process does not require mask, can be used for v arious rapid prototyping materials, to obtain high fabrication precision (its sc ale precision is 15 nm), and larger ratio of height to width of micro/nano-stru cture. It can find widespread applications in the fabrication of micro-mechani sm, trimming IC, and fabricating minilens, etc.

Impacts of Micro- and Nano-Plastics on Soil Properties and Plant Production in Agroecosystems: A Mini-Review

Micro- and nano-plastics (MNPs) are tiny plastic particles resulting from plastic product degradation. Soil MNPs have been identified as potential influential factors affecting various soil properties and crop biomass productivity. This mini-review provides a synthesis of recent findings concerning their effects on soil physicochemical properties, microorganisms, organic carbon content, soil nutrients, greenhouse gas emissions, soil fauna, and their impacts on plant ecophysiology, growth, and production. The results indicate that MNPs may markedly impede soil aggregation ability, increase porosity, decrease soil bulk density, enhance water retention capacity, influence soil pH and electrical conductivity, and escalate soil water evaporation. Exposure to MNPs may predominantly induce changes in soil microbial composition, reducing the diversity and complexity of microbial communities and microbial activity while enhancing soil organic carbon stability, influencing soil nutrient dynamics, and stimulating organic carbon decomposition and denitrification processes, leading to elevated soil respiration and methane emissions, and potentially decreasing soil nitrous oxide emission. Additionally, MNPs may adversely affect soil fauna, diminish seed germination rates, promote plant root growth, yet impair plant photosynthetic efficacy and biomass productivity. These findings contribute to a better understanding of the impacts and mechanistic foundations of MNPs. Future research avenues are suggested to further explore the impacts and economic implications.

Autophagy mediates osteoporotic bone regeneration induced by micro-/nano-structured modification on hydroxyapatite bioceramics

Osteoporosis(OP)is an age-related disease of bone metabolism,characterized by bone mass loss and bone mi-croarchitecture deterioration,the poor osteogenesis microenvironment of OP caused hardly repairing of the bone defects.As a dynamic process to fuel cellular renovation,autophagy has been proved to play a vital role in regulating cell differentiation and maintaining bone homeostasis.Traditional bone repairing biomaterials are hardly repairing the bone defects under OP pathological microenvironment.Therefore,it is essential to develop-ment novel biomaterials to improve osteoporotic osteogenesis.Compared to biochemical cues,biophysical cues exhibited more advantages in biocompatible and side effects.Herein,inspired by the importance of enhanced au-tophagic response in osteoporotic environment,we intend to utilize the micro-/nano-structured hydroxyapatite(mnHA)bioceramics as the mimic structure of natural bone tissue to regulate autophagic activity in ovariectomy bone mesenchymal stem cells(OVX-BMSCs),finally promote to bone regeneration in OP condition.The results indicated that mnHA bioceramics promoted cell adhesion and osteogenesis of OVX-BMSCs,and enhanced au-tophagy level in OVX-BMSCs.In the calvarial defects of OVX-rats,the mnHA scaffold acquired excellent bone repair effect.According to the current findings,regulating the level of autophagy could be a promising strategy for improve osteoporotic osteogenesis in the future.

Al_2O_3-Y_2O_3 Nano- and Micro-Composite Coatings on Fe-9Cr-Mo Alloy

Al2O3-Y2O3 nano- and micro-composite coatings were deposited on Fe-9Cr-Mo substrates by using sol-gel composite coating technology. The processing includes dipping samples in a sol-gel solution dispersed with fine ceramic powders, which are prepared by high-energy ball milling. High-resolution microscopy （FE-SEM） analyses show that the coating is composed of composite particle clusters with an average diameter of 1μm, and the coating is relatively dense without cracking during drying and sintering stages. XRD analyses show that the oxide coating is mainly composed of α-Al2O3 and γ-Al2O3. The oxidation tests performed at 600℃ in air show that the coatings are provided with much improved resistance against high temperature oxidation and scale spallation. It is indicated that nano-structured composite particles and reactive elements are integrated into the coatings, which plays an important role in preventing agglomeration of nano-particles and initiation of cracks.

Preparation and Mechanical Properties of Micro- and Nano-sized SiC/Fluoroelastomer Composites

Microand nano-sized SiC/fluoroelastomer （FKM） composites were prepared by a mechanical mixing method. These composites were first characterized by a rotorless rheometer. Then the effects of micro- and nano-sized SiC on hardness, static and dynamic mechanical properties of the composites were investigated. The increasing amount of the SiC filler increased the curing efficiency of the biphenyl curing system, which was evident from the rheometric properties of the resulting composites. The tensile properties of composite increased with the increasing of micro- and nano-sized SiC content. When the micro- and nano-sized SiC content was higher than 20 phr, the composites showed almost unchanged tensile properties. The increasing of the tensile property was mainly attributed to the well dispersed micro- and nano-sized SiC particles characterized by SEM images. Compared to pure FKM, the composites exhibited a higher glass transition temperature and lower tan peak value.

TPU/Nano-ZnO复合改性沥青的性能研究及微观机制

为促进聚合物/纳米改性沥青在耐久性路面中的应用,在实验室将不同掺量的聚氨酯及纳米氧化锌添加到A-70#基质沥青中制备了复合改性沥青。采用传统物理性能试验、动态剪切流变试验(DSR)、弯曲梁流变试验(BBR)研究了其物理性能与流变特性,基于响应面法的优化设计来明确两种改性剂的最佳掺量。借助傅里叶红外光谱试验(FTIR)对其微观改性机理进行探讨。采用高压紫外汞灯环境箱对改性沥青进行不同时间的紫外老化,分析其抗紫外老化能力,并基于主成分分析法评价了老化性能测试指标的显著性。结果表明:聚氨酯与纳米氧化锌的共同作用提高了基质沥青的高温稳定性及低温抗裂性,两种改性剂的最佳掺量分别为5%、3%。根据FTIR结果,复合改性沥青的改性过程既存在物理共混,又有化学加成反应。聚氨酯及纳米氧化锌的加入在基质沥青紫外老化过程中能够抑制羰基、亚砜基等极性基团的生成,复数剪切模量、羰基指数、劲度模量及亚砜基指数对沥青紫外老化性能的影响最为显著。

Elastic modulus of claystone evaluated by nano-/micro-indentation tests and meso-compression tests

Toarcian claystone such as that of the Callovo-Oxfordian is a qualified multiphase material. The claystone samples tested in this study are composed of four main mineral phases: silicates(clay minerals, quartz,feldspars, micas)(z86%), sulphides(pyrite)(z3%), carbonates(calcite, dolomite)(z10%) and organic kerogen(z1%). Three sets of measurements of the modulus of deformability were compared as determined in(i) nanoindentation tests with a constant indentation depth of 2 mm,(ii) micro-indentation tests with a constant indentation depth of 20 mm, and(iii) meso-compression tests with a constant displacement of 200 mm. These three experimental methods have already been validated in earlier studies. The main objective of this study is to demonstrate the influence of the scaling effect on the modulus of deformability of the material. Different frequency distributions of the modulus of deformability were obtained at the different sample scales:(i) in nano-indentation tests, the distribution was spread between 15 GPa and 90 GPa and contained one peak at34 GPa and another at 51 GPa;(ii) in the micro-indentation tests, the distribution was spread between 25 GPa and 60 GPa and displayed peaks at 26 GPa and 37 GPa; and(iii) in the meso-compression tests, a narrow frequency distribution was obtained, ranging from 25 GPa to 50 GPa and with a maximum at around 35 GPa.

In-situ hydrocarbon formation and accumulation mechanisms of micro- and nano-scale pore-fracture in Gulong shale, Songliao Basin, NE China

By conducting experimental analyses, including thermal pyrolysis, micro-/nano-CT, argon-ion polishing field emission scanning electron microscopy (FE-SEM), confocal laser scanning microscopy (CLSM), and two-dimensional nuclear magnetic resonance (2D NMR), the Gulong shale oil in the Songliao Basin was investigated with respect to formation model, pore structure and accumulation mechanism. First, in the Gulong shale, there are a large number of pico-algae, nano-algae and dinoflagellates, which were formed in brackish water environment and constituted the hydrogen-rich oil source materials of shale. Second, most of the oil-generating materials of the Qingshankou Formation shale exist in the form of organo-clay complex. During organic matter thermal evolution, clay minerals had double effects of suppression and catalytic hydrogenation, which expanded shale oil window and increased light hydrocarbon yield. Third, the formation of storage space in the Gulong Shale was related to dissolution and hydrocarbon generation. With the diagenesis, micro-/nano-pores increased, pore diameter decreased and more bedding fractures appeared, which jointly gave rise to the unique reservoir with dual media (i.e. nano-scale pores and micro-scale bedding fractures) in the Gulong shale. Fourth, the micro-/nano-scale oil storage unit in the Gulong shale exhibits independent oil/gas occurrence phase, and shows that all-size pores contain oils, which occur in condensate state in micropores or in oil-gas two phase (or liquid) state in macropores/mesopores. The understanding about Gulong shale oil formation and accumulation mechanism has theoretical and practical significance for advancing continental shale oil exploration in China.

A Status Graph Based Control Allocation Algorithm of Digital Micro-Thruster Array for Micro/Nano-Satellites Orbit Control Application

Digital micro-thruster arrays can be used for special missions of micro/nano-satellites with the requirements of high precision and small impulse.This paper presents a novel control allocation algorithm for the digital micro-thruster array,namely status graph based control allocation(SGBCA)algorithm,which aims at finding the optimal micro thrusters combination scheme to realize the sequential control synthesis for micro/nano-satellite during real-time orbit control tasks.A mathematical model is set up for the control allocation of this multivariate over-actuated system.Through dividing thrusters into disjoint segments by offline calculation and combining segments dynamically online to provide a sequence of the required impulse for the micro/nano-satellite,the time complexity of the control allocation algorithm decreases significantly.All levels of impulse can be generated by the digital micro thruster arrays and the service life of the arrays can be extended using the segment converting strategy proposed in this paper.The simulation indicates that the algorithm can satisfy the requirements of real-time orbit control for micro/nano-satellites.

TC4合金表面Cu/micro-WC复合镀层和Cu/nano-WC复合镀层的性能

在TC4合金表面分别制备了Cu/micro-WC复合镀层和Cu/nano-WC复合镀层。比较了两种复合镀层的表面形貌、化学成分和显微硬度,同时分析了两种复合镀层的摩擦特性。结果表明:两种复合镀层都由Cu、W、C元素组成,显微硬度都明显低于TC4合金的显微硬度;摩擦试验前后,两种复合镀层表面轮廓曲线的形态都存在明显的不同;与Cu/micro-WC复合镀层相比,Cu/nano-WC复合镀层的表面形貌较好,W元素的质量分数较高,耐磨性较强。

Determinationofelasticmodulus of claystone:Nano-/micro-indentation andmeso-compression tests used to investigate impact of alkaline fluid propagation over 18 years

Micro-mechanical properties of a claystone were tested after undergoing alkaline perturbation on site(Tournemire,CD borehole)for 18 years.In a saturated context and outside the excavation disturbed zone(EDZ),the claystone exhibits a 11.6-mm black rim at the cement/paste interface,which shows a different mechanical behaviour from the rest of the claystone.Three sets of measurements of elastic modulus were performed using:(i)nano-indentation tests with a constant indentation depth of 2 mm,(ii)microindentation tests with a constant indentation depth of 20 mm,and(iii)meso-compression tests with a constant displacement of 200 mm.The increase of the modulus of deformability in the black rim is between 15 GPa and 20 GPa according to the scale.Moreover,an overall decrease of the modulus of deformability from the smallest to the largest scale is observed in each zone.In view of the mineralogy and petrographic observations,higher values of modulus of deformability in the black rim are related to carbonate content and its distribution.Precipitation of cementitious carbonates as inclusions and very thin partings leads to hardening of the claystone.

Shugan Jieyu capsule effects on peripheral blood micro-124, micro- 132, and brain-derived neurotrophic factor in patients with mild to moderate depression

BACKGROUND To assess the effectiveness of Shugan Jieyu capsules on peripheral blood miR-124,miR-132,and brain-derived neurotrophic factor(BDNF)levels in patients with mild to moderate depression following coronary artery intervention[percuta-neous coronary intervention(PCI)]for coronary heart disease.Patients with mild-to-moderate depression of the liver-qi stagnation type after PCI for coronary heart disease at the 305th Hospital of the People’s Liberation Army were enrolled from June 2022 to November 2023 and randomly assigned to two groups:Experimental(treated with Shugan Jieyu capsules)and control(tr-eated with escitalopram oxalate tablets).This study compared the antidepressant effects of these treatments using 17-item Hamilton Rating Scale for Depression(HAMD-17)scores,metabolic equivalents,low-density lipoprotein cholesterol,BDNF,high-sensitivity C-reactive protein levels,miR-124 and miR-132 levels,distribution of immune-related lymphocyte subsets,and traditional Chinese me-dicine syndrome scores before and after 6 weeks of treatment.RESULTS No significant difference was observed in any index between the two groups before treatment(P>0.05).After treatment,the total efficacy rates were 93.33%and 90.00%in the experimental and control groups,respectively.Experimental group had significantly lower scores for the main and secondary syndromes compared to the control group(P<0.05).No significant difference was observed in the metabolic equivalents between the two groups be-fore and after treatment(P>0.05).The levels of low-density lipoprotein cholesterol,high-sensitivity C-reactive pro-tein,and miR-132 were significantly lower,whereas those of miR-124,BDNF,CD3+T lymphocytes,CD3+CD4+T helper lymphocytes,and CD3+CD4+/CD3+CD8+cells were significantly higher in the experimental group com-pared to the control group(P<0.05).The incidence of adverse reactions during experimental group was signi-ficantly lower than that in control group(P<0.05).CONCLUSION Shugan Jieyu capsules have good efficacy in patients with mild-to-moderate depression after PCI,and its me-chanism may contribute to the regulation of miR-124,miR-132,BDNF levels,and lymphoid immune cells.

Modification of Nano-α-Al2O3 and Its Influence on the Surface Properties of Waterborne Polyurethane Resin Composite Passivation Films

Silane coupling agent KH560 was used to modify the surface of nano-α-Al2O3 in ethanol-aqueous solution with different proportions. The particle size of nano-α-Al2O3 was determined by nano-particle size analyzer, and the effects of nano-α-Al2O3 content, ethanol-aqueous solution ratio and KH560 dosage on the dispersion and particle size of nano-α-Al2O3 were investigated. The material structure before and after modification was determined by Fourier transform infrared spectroscopy (FTIR). Aqueous polyurethane resin and inorganic components are combined with modified nano-α-Al2O3 dispersion to form chromium-free passivation solution. The solution is coated on the galvanized sheet, the adhesion and surface hardness are tested, the bonding strength of the coating and the surface hardness of the substrate are discussed. The corrosion resistance and surface morphology of the matrix were investigated by electrochemical test, neutral salt spray test and scanning electron microscope test. The chromium-free passivation film formed after the modification of nano-α-Al2O3 increases the surface hardness of galvanized sheet by about 85%. The corrosion resistance of the film is better than that of a single polyurethane film. The results show that the surface hardness and corrosion resistance of polyurethane resin composite passivation film are significantly improved by the introduction of nano-α-Al2O3.

Oil Pollutants Degradation of Nano-MgO in Micro-Polluted Water

The removal of oil pollutants from water and purifying process of oil-polluted water are studied through catalytic degradation method with nano-MgO. The results indicated that catalytic degradation effect of nano-MgO on the oil pollutants was associated with dosage of nano-MgO, pH and water temperature. When oil content was 1.8 mg/L, 0.17 g nano-MgO was used and the removal rate of oil was 93.92%. Furthermore, nano-Mgo was a non-photosensitive catalyst. GC/MS analysis showed that the amount of petroleum-based pollutants in water was reduced 73.77% from the previous 61 kinds to 16 kinds, and the total peak area was reduced 96.05% after catalytic degradation of nano-MgO. Therefore, nano-MgO has an excellent effect on the catalytic degradation of oil pollutants and can be applied in the treatment of oil wastewaters.

基于刻蚀处理的池沸腾传热性能实验研究

为探究刻蚀处理铜板表面对池沸腾传热性能的改善效果,搭建了可视化实验平台,制备不同刻蚀浓度和刻蚀时间处理的表面,采用去离子水为工质,进行饱和池沸腾实验.结果表明,氨水刻蚀浓度越大,表面的换热性能越强,氨水刻蚀时间越长,表面换热性能越强.热流密度为43.2 W/cm^(2)时,0.05 mol/L氨水刻蚀4 h表面、0.05 mol/L氨水刻蚀5 h表面、0.05 mol/L氨水刻蚀8 h表面、0.03 mol/L氨水刻蚀4 h表面、0.05 mol/L氨水刻蚀4 h表面和0.10 mol/L氨水刻蚀4 h表面上的换热系数分别提高了46%、92%、105%、36%、45%、78%.此外,对其表面进行表征,结果说明了其优异的沸腾性能.刻蚀处理增强了表面粗糙度,有效面积,从而促进液体的补充,能够在较小的过热度下达到更高的临界热流密度.可视化研究结果表明,刻蚀表面具有更多的成核位点,更小的气泡离开直径和离开频率.这些结果显示出刻蚀表面强化池沸腾换热的潜力.

Nano-diamond/Ni复合电刷镀层的组织形貌的研究

以45#钢为基体,制备了普通镍镀层和nano-diamond/Ni复合刷镀层,采用扫描电镜、能谱仪、X射线衍射仪和金相显微镜等设备对比研究了两种镀层的组织形貌。金相显微镜和扫描电镜分析结果表明,与普通镍镀层相比,nano-diamond/Ni复合刷镀层的表面更加平整、致密,组织更加细化;nano-dia-mond/Ni复合刷镀层表面呈典型的"菜花头"形状,弥散分布的纳米金刚石被镍包裹。能谱仪分析结果表明,nano-diamond/Ni复合刷镀层中纳米颗粒特征元素C的质量分数为7.37%。X射线衍射分析结果表明,随着纳米金刚石加入量的增加,镀层表面晶粒尺寸逐渐减小。

微波辐射KF/nano-γ-Al_2O_3催化的Gewald反应研究

以KF/nano-γ-Al2O3为碱性催化剂,在微波辐射作用下发生三组分Gewald反应,合成了一类2-氨基噻吩类化合物,并探索了不同反应条件对收率的影响。实验结果表明,该方法具有催化剂廉价高效、反应时间短、产品收率高、后处理简便等优点。

Effect of a Hybrid Micro/Nano-integrated Titanium Surface on Behavior of Rat Osteoblasts

The objective of this study was to investigate the effect of a new combined micro/nanoscale implant surface feature on osteoblasts＇ behaviors including cell morphology, adhesion, proliferation, differentiation, and mineralization in vitro. A new micro/nano-hybrid topography surface was fabricated on commercial pure titanium（Cp Ti） by a two-step sandblasted acid-etching and subsequent alkali-and heattreatment（SA-AH）. The conventional sandblasted/acid-etching（SA） treatment and alkali and heat（AH） treatment were also carried out on the Cp Ti as controls. Surface microstructures of the Ti disc samples were assessed by scanning electron microscopy（SEM）. The neonatal rat calvaria-derived osteoblasts were seeded on these discs and the initial cell morphology was evaluated by SEM and immunofluorescence. Initial adhesion of the cells was then assayed by DAPI staining at 1, 2, and 4 h after seeding. The Cell Counting Kit-8（CCact K8） assay, gene expression of osteoblastic markers（ALP, Col 1, OCN, BSP, OSX, Cbfα1） and Alizarin Red S staining assays were monitored respectively for cell proliferations, differentiation and mineralization. The results show significant differences in osteoblast＇s behaviors on the four kinds of Ti surfaces. Compared with Cp Ti surface, the SA and AH treatment can significantly promote cell adhesion, differentiation and mineralization of osteoblasts. In particular, the combined SA and AH treatments exhibit synergistic effects in comparison with the treatment of SA and AH individually, and are more favorable for stimulating a series of osteogenous responses from cell adhesion to mineralization of osteoblasts. In summary, this study provides some new evidence that the integrated micro/nanostructure on the Cp Ti surface may promote bone osseointegration between the Ti implantbone interfaces in vitro.

纳米金颗粒@介孔二氧化硅修饰钛种植体促进高糖条件下的成骨分化

背景:微纳米结构改性是钛种植体表面处理的热点研究领域。糖尿病高血糖环境会影响钛种植体与骨组织形成稳定的结合,探索通过表面微纳结构改性来提高钛种植体在高糖环境下的成骨活性是有必要的。目的:观察钛表面纳米金颗粒@介孔二氧化硅涂层对体外高糖条件下成骨分化的影响。方法:分别制备纳米金颗粒悬浮液与介孔二氧化硅,将二者按一定比例混合在去离子水中,制备纳米金颗粒@介孔二氧化硅悬浮液。取钛片,分3组处理:光滑组经过水砂纸打磨处理,纳米管组经过水砂纸打磨处理后采用阳极氧化技术制备二氧化钛纳米管涂层,实验组制备二氧化钛纳米管涂层后浸泡于纳米金颗粒@介孔二氧化硅悬浮液中,制备纳米金颗粒@介孔二氧化硅涂层,表征3组钛片表面的微观形貌、亲水性。将大鼠骨髓间充质干细胞接种于3组钛片表面,通过细胞活/死荧光染色及CCK-8实验检测细胞增殖,DAPI/鬼笔环肽染色检测细胞黏附。将大鼠骨髓间充质干细胞接种于3组钛片表面,加入高糖成骨诱导培养基培养,通过碱性磷酸酶与茜素红S染色检测成骨分化。结果与结论:(1)扫描电镜下可见光滑组钛片表面均匀平坦,纳米管组钛片表面排列紧密的二氧化钛纳米管阵列,实验组钛片在二氧化钛纳米管表面及内部均负载纳米金颗粒@介孔二氧化硅;纳米管组、实验组钛片亲水性均优于光滑组。(2)细胞活/死荧光染色结果显示,3组钛片表面的细胞活力均高于90%;CCK-8实验结果显示,实验组细胞增殖率高于光滑组、纳米管组;DAPI/鬼笔环肽染色结果显示,二氧化钛纳米管涂层、纳米金颗粒@介孔二氧化硅涂层更有利于细胞黏附。(3)碱性磷酸酶与茜素红S染色结果显示,实验组钛片表面细胞碱性磷酸酶活性与细胞外基质矿化程度均高于光滑组、纳米管组。(4)结果表明,纳米金颗粒@介孔二氧化硅涂层可增强钛表面生物活性,促进高糖环境下的成骨分化。