This paper described a highly sensitive chemiluminescence detection system for micro-chip electrophoresis (MCE) based on luminol-hydrogen peroxide reaction catalyzed by the metal ions. The micro-chip was composed of p...This paper described a highly sensitive chemiluminescence detection system for micro-chip electrophoresis (MCE) based on luminol-hydrogen peroxide reaction catalyzed by the metal ions. The micro-chip was composed of poly(dimethylsiloxane) (PDMS) and glass, and was fabricated by micro-machining technology. The surface of channels was dynamically modified by polydimethylacrylamide (PDMA) in order to eliminate unhomogeneous electroosmotic flow (EOF) of the PDMS/glass chip, adsorption of molecules, and improve hydrophobicity on PDMS surface. The detection modes, reagent mix procedures and reaction conditions were optimized and the detection limit of 5×10 mol/L for cobalt (II) was achieved by MCE with chemiluminescence detection, which was about four orders of magnitude more sensitive than that reported in the reference.展开更多
我国是慢性病大国,癌症、糖尿病、心脏病、癫痫、高血压等疾病已成为首要致死原因,医疗负担占比70%,形势严峻.相比口服,注射等传统给药方式,植入式精准靶向治疗按时按需、精准适量,见效迅速,几乎无副作用,被证实是慢性病的有效治疗方式...我国是慢性病大国,癌症、糖尿病、心脏病、癫痫、高血压等疾病已成为首要致死原因,医疗负担占比70%,形势严峻.相比口服,注射等传统给药方式,植入式精准靶向治疗按时按需、精准适量,见效迅速,几乎无副作用,被证实是慢性病的有效治疗方式.目前我国在这一领域刚刚起步,尤其在高端植入微纳集成系统级芯片(System of Chip,SoC)、微纳系统集成和封装可靠性等方面,缺少原创性核心技术和共性技术支撑平台,严重落后于欧美发达国家,相关高端医疗器械及核心部件只能高价进口.因此,需要通过突破相关关键共性技术,核心部件国产化,从创新源头推动高端医疗器械发展,从而加速推动我国医疗器械产业化落地,打破欧美日发达国家垄断.本研究以突破医疗用微纳集成芯片和微纳集成系统关键共性技术为目的,解决了微电子小尺寸、高精度、低功耗等难题.通过三维异质集成构建植入式精准靶向给药系统和迷走神经刺激器,实现精准靶向给药、无线充电、生物电信号采集、电刺激等功能,项目样机已通过生物相容性测试和动物实验验证.展开更多
文摘This paper described a highly sensitive chemiluminescence detection system for micro-chip electrophoresis (MCE) based on luminol-hydrogen peroxide reaction catalyzed by the metal ions. The micro-chip was composed of poly(dimethylsiloxane) (PDMS) and glass, and was fabricated by micro-machining technology. The surface of channels was dynamically modified by polydimethylacrylamide (PDMA) in order to eliminate unhomogeneous electroosmotic flow (EOF) of the PDMS/glass chip, adsorption of molecules, and improve hydrophobicity on PDMS surface. The detection modes, reagent mix procedures and reaction conditions were optimized and the detection limit of 5×10 mol/L for cobalt (II) was achieved by MCE with chemiluminescence detection, which was about four orders of magnitude more sensitive than that reported in the reference.
文摘我国是慢性病大国,癌症、糖尿病、心脏病、癫痫、高血压等疾病已成为首要致死原因,医疗负担占比70%,形势严峻.相比口服,注射等传统给药方式,植入式精准靶向治疗按时按需、精准适量,见效迅速,几乎无副作用,被证实是慢性病的有效治疗方式.目前我国在这一领域刚刚起步,尤其在高端植入微纳集成系统级芯片(System of Chip,SoC)、微纳系统集成和封装可靠性等方面,缺少原创性核心技术和共性技术支撑平台,严重落后于欧美发达国家,相关高端医疗器械及核心部件只能高价进口.因此,需要通过突破相关关键共性技术,核心部件国产化,从创新源头推动高端医疗器械发展,从而加速推动我国医疗器械产业化落地,打破欧美日发达国家垄断.本研究以突破医疗用微纳集成芯片和微纳集成系统关键共性技术为目的,解决了微电子小尺寸、高精度、低功耗等难题.通过三维异质集成构建植入式精准靶向给药系统和迷走神经刺激器,实现精准靶向给药、无线充电、生物电信号采集、电刺激等功能,项目样机已通过生物相容性测试和动物实验验证.
