Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury

Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord injury.Microglia and astrocytes play key roles in the spinal cord injury micro-environment and share a close interaction.However,the mechanisms involved remain unclear.In this study,we found that after spinal cord injury,resting microglia(M0)were polarized into pro-inflammatory phenotypes(MG1 and MG3),while resting astrocytes were polarized into reactive and scar-forming phenotypes.The expression of growth arrest-specific 6(Gas6)and its receptor Axl were significantly down-regulated in microglia and astrocytes after spinal cord injury.In vitro experiments showed that Gas6 had negative effects on the polarization of reactive astrocytes and pro-inflammatory microglia,and even inhibited the cross-regulation between them.We further demonstrated that Gas6 can inhibit the polarization of reactive astrocytes by suppressing the activation of the Yes-associated protein signaling pathway.This,in turn,inhibited the polarization of pro-inflammatory microglia by suppressing the activation of the nuclear factor-κB/p65 and Janus kinase/signal transducer and activator of transcription signaling pathways.In vivo experiments showed that Gas6 inhibited the polarization of pro-inflammatory microglia and reactive astrocytes in the injured spinal cord,thereby promoting tissue repair and motor function recovery.Overall,Gas6 may play a role in the treatment of spinal cord injury.It can inhibit the inflammatory pathway of microglia and polarization of astrocytes,attenuate the interaction between microglia and astrocytes in the inflammatory microenvironment,and thereby alleviate local inflammation and reduce scar formation in the spinal cord.

Seasonal dynamics of gas regulation service in forest ecosystem

Using the 3-year observational data from ChinaFlux （Chinese Terrestrial Ecosystem Flux Research Network）, we studied the gas regulation flux dynamics and cumulative process of gas regulation value in Qianyanzhou middle subtropical plantation （QYF） and Changbai Mountain temperate mixed forest （CBF）. The gas regulation service was differentiated into vegetation gas regulation service and net ecosystem gas regulation service. Carbon tax approach, reforestation cost approach and industrial oxygen approach were employed to calculate gas regulation value. Results show that there was significant seasonal variation in vegetation gas regulation flux. Daily CO2 uptake fluxes averaged 82.00 kg·ha^-·d^-1 and 59.37 kg·ha^-·d^-1 and the corresponding 02 emission fluxes were 59.65 kg·ha^-·d^-1 and 43.19 kg·ha^-·d^-1 for QYF and CBF, respectively. The cumulative curves of vegetation gas regulation value always followed a sigmoid shape, and the annual gas regulation value produced by vegetation was RMB 14342.69 yuan·ha^-1 and RMB 10384.18 yuan·ha^-1 for both QYF and CBF, respectively. In terms of monthly net ecosystem gas regulation service, QYF appeared as a CO2 sink and O2 source for the whole year, while CBF appeared to be a CO2 sink and O2 source mainly in the period between May and September. The cumulative curves of net ecosystem gas regulation value presented a sigmoid （＂S＂） shape for QYF, while a unimodal type curve for CBF. The annual net ecosystem gas regulation value was 8470.52 yuan·ha^-1 and 5091.98yuan·ha^-1 for QYF and CBF, respectively. The economic value of both the vegetation gas regulation service and net ecosystem gas regulation service were mainly produced between May and October.

Economic valuation of gas regulation as a service by rice-duck-fish complex ecosystem

Valuating the fimction of ecosystem services is crucial for accounting green GDP, making a conservation policy of ecological environment and the decision of regional development as well as sustainable development strategy. R ice-duck-fish symbiosis has been promoted in several developing countries as a way of increasing incomes for rice farmers, but investigations of its value have mainly focused on direct economic＇ benefits, such as food and raw material production. Few studies have been conducted on the estimation of indirect services provided by rice-duck-fish complex ecosystem. The gas regulation service and its economic values provided by rice-duck-fish complex ecosystem were studied in Wuhan, China. The major components of gas regulation are O2 emission and greenhouse gases （GHGs, CO2） regulation. The results show that O2 emission from different treatments （including rice-duck （RD）, rice-fish （RF）, rice-duck-fish （RDF） and rice （CK）） ranged from 26,370 kg/ha to 33,910 kg/ha per year. with an economic value of 10,050-12,920 yuan/ha per ）,ear （Chinese currency： 1 euro =10.2475 yuan, August 28, 2007）. The net GHGs exchange varied from 1,200 to 3,320 kg/ha per year; and its economic value ranged from 1,040 yuan/ha to 2,900 yuan/ha per year Consequently, the total economic value of gas regulation provided by symbiosis complex ecosystems ranged from 11,090 yuan/ha to 15,820 yuan/ha per year. and the maximum overall economic value of gas regulation was provided by RDF complex ecosystem. The work will be useful for further understanding of the functions of rice-duck-fish complex ecos.vstem services and supplying the theoretical references to agricultural policy.

Effect of integration of mechanical ball milling and flue gas desulfurization gypsum on dealkalization of bauxite residue

The synergistic impact of mechanical ball milling and flue gas desulfurization(FGD)gypsum on the dealkalization of bauxite residue was investigated through integrated analyses of solution chemistry,mineralogy,and microtopography.The results showed a significant decrease in Na_(2)O content(>30 wt.%)of FGD gypsum-treated bauxite residue after 30 min of mechanical ball milling.Mechanical ball milling resulted in differentiation of the elemental distribution,modification of the minerals in crystalline structure,and promotion in the dissolution of alkaline minerals,thus enhancing the acid neutralization capacity of bauxite residue.5 wt.%FGD gypsum combined with 30 min mechanical ball milling was optimal for the dealkalization of bauxite residue.

Regulations and Practice on Flue Gas Denitrification for Coal-Fired Power Plants in China

In China, according to the relative up-to-date regulations and standards, the maincontrol measure for NOX emission of coal-fired power plants is, in principle, low NOXcombustion. However, in recent years, more and more newlyapproved coal-fired plantswere required to install flue gas denitrification equipment. This article expounds if fluegas denitrification is necessary from several aspects, including constitution of NOX, itsimpact to environment, operation ofdeNOXequipment in USA, as wellas the differencein ambient air quality standard between China and World Health Organization. It setsforth themes in urgent need of study and areas where deNOX equipment is necessaryfor new projects, besides a recommendation that the emission standards for thermalpowerplants should be revised as soon as possible in China.

Experimental and Numerical Study on the Gas Explosion in Urban Regulator Station

Regulator station is an important part in the urban gas transmission and distribution system.Once gas explosion occurs,the real explosion process and consequences of methane gas explosion in the regulator station were not revealed systematically.In this study,a full-scale experiment was carried out to simulate the regulator station explosion process,and some numerical simulations with a commercial CFD software called FLACS were conducted to analyze the effect of ignition and vent conditions on the blast overpressure and flame propagation.The experimental results demonstrated that the peak overpressure increased as the distance from the vent increased within a certain distance.And the maximum overpressure appeared 3 m away from the door,which was about 36.6 kPa.It was found that the pressure-time rising curves obtained from the simulation are basically the same as the ones from the experiment,however,the time of reaching the peak pressure was much shorter.The numerical simulation results show that the peak overpressures show an increase trend as the ignition height decreased and the vent relief pressure increased.It indicates that the damage and peak overpressure of gas explosion could be well predicted by FLACS in different styles of regulator station.In addition,the results help us to understand the internal mechanism and development process of gas explosion better.It also offers technical support for the safety protection of the urban regulator station.

Integrated and dual-responsive lipopeptide nanovector with parallel effect to tumor and micro-environment regulation by efficient gene and drug co-delivery

The integrated lipopeptide(RVA)/gene complexes are fabricated with bi-directional regulation on tumor cells and micro-environment.After self-assembling and target coating modification,the poly(γ-glutamic acid)(γ-PGA)/RVA nano-vectors can sequentially respond to pH&redox stimuli,and guarantee efficient therapeutic gene delivery and control release of all-trans retinoic acid.The design provides a facile but promising strategy to treat refractory cancers.

Study on Production Regulation of Reservoir under Different Demand

Natural gas production is related to the demand for gas, which is low in summer and high in winter. While the gas storage is still being demonstrated and constructed, oil and gas fields should formulate and implement production control schemes suitable for gas reservoirs. The realization of natural gas production can not only meet the demand of gas consumption, but also ensure the scientific and efficient development of gas reservoirs, and meet the needs of dynamic analysis of gas reservoirs at different development stages and scientific research of gas reservoirs. In this paper, KAPPA Workstation 5.20 software is used to determine the inflow dynamic model of a single well. The nodal method is used to determine the reasonable production and peak shaving capacity in combination with the critical fluid carrying capacity of gas wells and the erosion rate of gas wells. The reasonable production allocation in each period, i.e. the production control scheme, is obtained. It solves the scientific and efficient development of natural gas in X gas field, which is still under the construction of gas storage, and provides guidance for gas reservoir development management and regulation.

Investigation of Yield Regulation of Red Grapes on the Concentration of Some Crucial Wine Components

An essential concept of making quality wine is that the quality comes from the vineyard. The dissolved materials and components determine the value of wine. Their amount and proportion are essentially influenced by grape variety, terroir, the way of cultivation, weather conditions, grape quality, the time of vintage and the method of grape and wine processing. In the present study the effect on wine quality of different ways of yield regulations was examined in wines of three consecutive years. Besides basic parameters, total polyphenol and resveratrol contents were determined in wines of Blaufr?nkisch and Merlot varieties. Our measurements were carried out by spectrophotometry, gas and liquid chromatography. Our investigations confirmed that yield regulation led to increased extract content and outstanding quality of the produced wine.

Numerical Simulation of Oil and Gas Two-Phase Flow in Deep Condensate Gas Reservoirs in Bohai Buried Hills

The BZ19-6 gas field is characterized by high temperature and high pressure (HTHP), high condensate content, little difference between the formation pressure and dew point pressure, and large amount of reverse condensate liquid. During the early stage of depletion development, the production gas-oil ratio (GOR) and production capacity remain relatively stable, which is inconsistent with the conventional reverse condensate seepage law. In view of the static and dynamic conflict in development and production, indoor high-temperature and high-pressure PVT experiment was carried out to reveal the mist-like condensation phenomenon of fluids in the BZ19-6 formation. And the seepage characteristics of condensate gas reservoirs with various degrees of depletion under the condition of HTHP were analyzed based on production performance. The change rule of fluid phase state was analyzed in response to the characterization difficulties of the seepage mechanism. The fluid state was described using the miscible mechanism. And the interphase permeability interpolation coefficient was introduced based on interfacial tension. By doing so, the accurate characterization of the “single-phase flow of condensate gas-near-miscible mist-like quasi single-phase flow-oil-gas two-phase flow” during the development process was achieved. Then the accurate fitting of key indicators for oilfield development was completed, and the distribution law of formation pressure and the law of condensate oil precipitation under different reservoir conditions are obtained. Based on research results, the regulation strategy of variable flow rate production was developed. Currently, the work system has been optimized for 11 wells, achieving a “zero increase” in the GOS of the gas field and an annual oil increase of 22,000 cubic meters.

An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 Regulates Petal Expansion in Rose

The speed of flower opening is closely related to their ornamental period.Ethylene functions as a negative regulator involved in the regulation of the petal expansion process.In this study,we isolated a NF-YC transcription factor gene,RhNF-YC9,fromrose petals.RhNF-YC9 expression was induced at the early stages of flower opening but was inhibited by ethylene treatment.Silencing RhNF-YC9 decreased the speed of petal expansion from stage 2 to stage 5.The expressions of 11 cell expansion-related genes involved in cell wall loosening,cell turgor modulation,and cytoskeleton remodeling were significantly down-regulated in RhNF-YC9-silenced petals.We also found that silencing RhNF-YC9 decreased the expression of gibberellin acid(GA)biosynthetic gene RhGA20ox while significantly increasing the transcripts of GA catabolic gene RhGA2ox,reducing the accumulation of GA4 and GA7.The influence of ethylene treatment on the expression of RhGA20ox and RhGA2ox showed the same trend.These results together suggested that RhNF-YC9 positively regulated the speed of petal expansion and mediated the crosstalk between ethylene and GA.Our findings revealed a new insight into the function of NF-YC transcription factors involved in ethylene-regulated petal expansion.

Examining the Effects of Environmental Policy on Shale Gas Production: The Case of Alberta, Canada

The increase in natural gas production in North America resulting from the implementation of new technologies related to the fracturing (fracking) of natural gas-bearing shale reservoirs has enhanced the security of supply and lowered energy costs in the continent. Yet the environmental impact associated with shale gas development has raised concerns and debate among energy and environmental policy makers as to how best to address these concerns. As Canada’s largest producer of natural gas, the Province of Alberta is an example of a jurisdiction with numerous regulations for dealing with such environmental risks. This paper applies the CO/RE model of Konschnik and Bolingin examining Alberta’s environmental regulatory framework and the impact;it will have on further shale gas production in the province. Aside from the identification of risks associated with increased seismicity, the results of this examination suggest that the current regulatory environment does not appear to have any adverse effect on current and future shale gas production within the province. Furthermore, Alberta’s environmental regulation has influenced shale gas producers to pursue innovation in technology and engineering practice and has helped establish a collaborative approach to mitigating environmental risk.

Strategies for Improving Gas Supplies for Sustainable Power Generation in Ghana

Technologies for gas conversion have attracted more serious attention, but energy efficiency, supply deficiencies, as well as other risks undermining the gas supply industry, make it difficult to achieve sustainability in gas supply in Ghana. This study explored strategies for improving supplies for sustainable power generation in Ghana. Specifically, the study investigates the risks associated with the gas supply industry and also investigates strategies for improving gas supplies in Ghana. A descriptive study that applied both qualitative and quantitative tools was applied to data collection and analysis to allow for an in-depth analysis of the findings of the research. The population was concentrated on the regulators (Energy Commission) and consumers (VRA and Sunon Asogli, and GNGC/Eni in the supply domains. In all, 357 population was targeted with a sample of 151. Probability (simple random sampling) and non-probability (purposive) sampling approaches were applied to source participants for the study. Statistical inferential tools that guided the analysis included means, standard deviation, Relative Importance Index (RII). The study identified issues of power sector debt due to the weak financial background of the gas supply sector, poor infrastructure, issues of corruption created by delays, poor transparency and weak regulatory framework as key risk factors in the gas supply industry in Ghana. The study also concluded that strategies to support the drive to achieve sustainability in the gas supply industry should involve a planned action towards providing regular and improved infrastructure in transportation and gas supply pipelines to enhance the visibility of the local gas industry and to also meet both domestic and industrial demand for gas products. Again, there should be the establishment and maintenance of functional gas-related policies and regulators, as well as an emergency supply plan to address any shocks that may tend to impact the gas supply industry in Ghana.

Using the EMD Method to Determine Fault Criterion for Medium-low Pressure Gas Regulators

By extracting the outlet pressure data of gas regulators,this paper uses the EMD toolbox of the MATLAB software,which can perform data decomposition and the Hilbert-Huang Transform to find the rules with fault data.Eventually,the medium-low pressure gas regulator fault criterion can be established.

Transcription regulation by DNA methylation under stressful conditions in human cancer

Background： We aim to address one question： do cancer vs. normal tissue cells execute their transcription regulation essentially the same or differently, and why？ Methods： We utilized an integrated computational study of cancer epigenomes and transcriptomes of 10 cancer types, by using penalized linear regression models to evaluate the regulatory effects of DNA methylations on gene expressions. Results： Our main discoveries are： （i） 56 genes have their expressions consistently regulated by DNA methylation specifically in cancer, which enrich pathways associated with micro-environmental stresses and responses, particularly oxidative stress; （ii） the level of involvement by DNA methylation in transcription regulation increases as a cancer advances for majority of the cancer types examined; （iii） transcription regulation in cancer vs. control tissue cells are substantially different, with the former being largely done through direct DNA methylation and the latter mainly done via transcriptional factors; （iv） the altered DNA methylation landscapes in cancer vs. control are predominantly accomplished by DNMTI, TET3 and CBX2, which are predicted to be the result of persistent stresses present in the intracellular and micro-environments of cancer cells, which is consistent with the general understanding about epigenomic functions. Conclusions： Our integrative analyses discovered that a large class of genes is regulated via direct DNA methylation of the genes in cancer, comparing to TFs in normal cells. Such genes fall into a few stress and response pathways. As a cancer advances, the level of involvement by direct DNA methylation in transcription regulation increases for majority of the cancer types examined.

Research on Online Safety Precaution Technology of a High-medium Pressure Gas Regulator

In view of some of the characteristics of a high-medium pressure gas regulator, such as small fault samples, many fault types and complex fault features etc, in order to improve the accuracy of fault precaution in this paper, a fault diagnosis method based on the combination of the improved wavelet packet and pressure harmonic distortion rate is proposed. On the basis of the harmonic distortion rate of the outlet pressure and the energy value of each frequency band obtained by improved wavelet packet decomposition, the rules for fault data were summarized. Finally, a safety precaution model of the high-medium pressure gas regulator is established.

Physiological responses and adjustment mechanisms of the dominate species of natural vegetation of Eastern Tengger Desert

Reaumuria soongorica and Salsola passerina have significant differences in ecophysiological characteristics, which change with the environmental variations. Although they live together for a long period of time, their adaptive mechanisms to environmental stresses are very different. As two extreme xerophytes, Reaumuria soongorica and Salsola passerina differ significantly from other psammophytes in ecophysiological characteristics; they can survive in lower water potential, and can even grow in piedmont areas. Low water potential may be related to the existence of osmosis-regulating substance, such as praline, which can strengthen the capacity of water absorption. Compared to other psammophytes, Reaumuria soongorica and Salsola passerina have a higher degree of photo-inhibition under the same condition, and the photo-inhibition can lead to destruction of the photosynthetic pigment, nevertheless, this photo-inhibition can be repaired under suitable conditions in the morning and evening.

Gas sensing selectivity of oxygen-regulated SnO2 films with different microstructure and texture

The selectivity of gas sensing materials is increasingly important for their applications. The oxygenregulated SnO2 films with(110) and(101) preferred orientation were obtained through magnetron sputtering, followed by annealing treatment. Their micro-structure, surface morphology and gas response were investigated by advanced structural characterization and property measurement. The results showed that the as-prepared(110)-oriented SnO2 film was oxygen-rich and had more adsorption sites while the as-prepared(101)-oriented SnO2 film was oxygen-poor and more sensitive to de-oxidation.H2 gas sensitivity, response speed, selectivity between H2 and CO of the(110)-orientated SnO2 film was superior to that of the(101)-orientated SnO2 film. After treated at high temperature and high vacuum,the reduction of gas-sensing properties of the annealed(110) SnO2 film was much more than that of the annealed(101) SnO2 film. The lattice oxygen was responsible for the difference in gas-sensing response between(110) and(101)-oriented SnO2 films under oxygen regulation. This work indicated the gassensing selectivity of the different crystal planes in SnO2 film, providing a significant reference for design and extension of the related materials.

Regulation of phagocytosis by TAM receptors and their ligands

The TAM family of receptors is preferentially expressed by professional and non-professional phagocytes,including macrophages,dendritic cells and natural killer cells in the immune system,osteoclasts in bone,Sertoli cells in testis,and retinal pigmental epithelium cells in the retina.Mutations in the Mertk single gene or in different combinations of the double or triple gene mutations in the same cell cause complete or partial impairment in phagocytosis of their preys;and as a result,either the normal apoptotic cells cannot be efficiently removed or the tissue neighbor cells die by apoptosis.This scenario of TAM regulation represents a widely adapted model system used by phagocytes in all different tissues.The present review will summarize current known functional roles of TAM receptors and their ligands,Gas 6 and protein S,in the regulation of phagocytosis.

Design and Availability Research of a Flammable and Explosive Volatiles Monitoring and Early Warning System (FEVMEW) for the Bus Crowded Places

In order to reduce the arson or accidental fire losses, we developed a gas sensitive detector used for the rapid detection and early warning of flammables in crowded places such as buses. A MEMS (Micro-Electro-Mechanical System) based thin film semiconductor was fabricated as the gas sensor. To obtain the target gas selective response, the surface of the sensitive film was modified with highly active metal catalytic nano-particles. Thus the anti-interference ability was improved and the false alarm rate was effectively reduced. Furthermore, the modular embedded system for information acquisition and transmission was developed. Supported by the Airflow Precision control system (APs), the rapid warning of volatile gas of flammable substances was realized. Experiments showed that RAs has satisfied selectivity to volatiles of usual flammable liquid, such as the output voltage reaches 3 V (0 - 3.3 V). With simulation about the actual installation state in bus, MWs sounds an alarm at 2 minutes after splashing 50 mL 92# petrol to the floor. For the last two years, FEVMEW has been integrated into more than 4000 buses in Hefei. This design has been proved feasible according to the actual operation.