Natural tissues contain highly organized cellular architecture.One of the major challenges in tissue engineering is to develop engineered tissue constructs that promote cellular growth in physiological directionality....Natural tissues contain highly organized cellular architecture.One of the major challenges in tissue engineering is to develop engineered tissue constructs that promote cellular growth in physiological directionality.To address this issue,micro-patterned polydimethylsiloxane(PDMS)substrates have been widely used in cell sheet engineering due to their low microfabrication cost,higher stability,excellent biocompatibility,and most importantly,ability to guide cellular growth and patterning.However,the current methods for PDMS surface modification either require a complicated procedure or generate a non-uniform surface coating,leading to the production of poor-quality cell layers.A simple and efficient surface coating method is critically needed to improve the uniformity and quality of the generated cell layers.Herein,a fast,simple and inexpensive surface coating method was analyzed for its ability to uniformly coat polydopamine(PD)with or without collagen on micro-grated PDMS substrates without altering essential surface topographical features.Topographical feature,stiffness and cytotoxicity of these PD and/or collagen based surface coatings were further analyzed.Results showed that the PD-based coating method facilitated aligned and uniform cell growth,therefore holds great promise for cell sheet engineering as well as completely biological tissue biomanufacturing.展开更多
Engineered targets are expected to play a key role in future high-power laser experiments calling for joined, extensive knowledge in materials properties, engineering techniques and plasma physics. In this work, we pr...Engineered targets are expected to play a key role in future high-power laser experiments calling for joined, extensive knowledge in materials properties, engineering techniques and plasma physics. In this work, we propose a novel patterning procedure of self-supported 10 μm thick Au and Cu foils for obtaining micrometre-sized periodic gratings as targets for high-power laser applications. Accessible techniques were considered, by using cold rolling, electronbeam lithography and the Ar-ion milling process. The developed patterning procedure allows efficient control of the grating and foil surface on large area. Targets consisting of patterned regions of 450 μm × 450 μm, with 2 μm periodic gratings, were prepared on 25 mm × 25 mm Au and Cu free-standing foils, and preliminary investigations of the microtargets interacting with an ultrashort, relativistic laser pulse were performed. These test experiments demonstrated that,in certain conditions, the micro-gratings show enhanced laser energy absorption and higher efficiency in accelerating charge particle beams compared with planar thin foils of similar thickness.展开更多
基金supported by the National Institutes of Health(1R15CA202656 and 1R15HL115521-01A1)the National Science Foundation(1703570)to FZ.
文摘Natural tissues contain highly organized cellular architecture.One of the major challenges in tissue engineering is to develop engineered tissue constructs that promote cellular growth in physiological directionality.To address this issue,micro-patterned polydimethylsiloxane(PDMS)substrates have been widely used in cell sheet engineering due to their low microfabrication cost,higher stability,excellent biocompatibility,and most importantly,ability to guide cellular growth and patterning.However,the current methods for PDMS surface modification either require a complicated procedure or generate a non-uniform surface coating,leading to the production of poor-quality cell layers.A simple and efficient surface coating method is critically needed to improve the uniformity and quality of the generated cell layers.Herein,a fast,simple and inexpensive surface coating method was analyzed for its ability to uniformly coat polydopamine(PD)with or without collagen on micro-grated PDMS substrates without altering essential surface topographical features.Topographical feature,stiffness and cytotoxicity of these PD and/or collagen based surface coatings were further analyzed.Results showed that the PD-based coating method facilitated aligned and uniform cell growth,therefore holds great promise for cell sheet engineering as well as completely biological tissue biomanufacturing.
基金supported by the ELI-NP Phase Ⅱ project,co-financed by the Romanian Government and the European Union through the European Regional Development Fund-the Competitiveness Operational Programme(contract No.1/07.07.2016,COP,ID 1334)Funding from the National Program‘Installations and Strategic Objectives of National Interest’and‘Nucleu’project PN19060105 of the Romanian Governmentfunding from the European Union Framework Programme for Research and Innovation Horizon 2020 under grant agreement No.871161.
文摘Engineered targets are expected to play a key role in future high-power laser experiments calling for joined, extensive knowledge in materials properties, engineering techniques and plasma physics. In this work, we propose a novel patterning procedure of self-supported 10 μm thick Au and Cu foils for obtaining micrometre-sized periodic gratings as targets for high-power laser applications. Accessible techniques were considered, by using cold rolling, electronbeam lithography and the Ar-ion milling process. The developed patterning procedure allows efficient control of the grating and foil surface on large area. Targets consisting of patterned regions of 450 μm × 450 μm, with 2 μm periodic gratings, were prepared on 25 mm × 25 mm Au and Cu free-standing foils, and preliminary investigations of the microtargets interacting with an ultrashort, relativistic laser pulse were performed. These test experiments demonstrated that,in certain conditions, the micro-gratings show enhanced laser energy absorption and higher efficiency in accelerating charge particle beams compared with planar thin foils of similar thickness.
