The most common methods for three-dimensional reconstruction of peripheral nerve fascicles include histological and radiology techniques. Histological techniques have many drawbacks including an enormous manual worklo...The most common methods for three-dimensional reconstruction of peripheral nerve fascicles include histological and radiology techniques. Histological techniques have many drawbacks including an enormous manual workload and poor image registration. Micro-magnetic resonance imaging（Micro-MRI）, an emerging radiology technique, has been used to report results in the brain, liver and tumor tissues. However, micro-MRI usage for obtaining intraneural structures has not been reported. The aim of this study was to present a new imaging method for three-dimensional reconstruction of peripheral nerve fascicles by ~1T micro-MRI. Freshly harvested sciatic nerve samples from an amputated limb were divided into four groups. Two different scanning conditions（Mannerist Solution/GD-DTPA contrast agent, distilled water） were selected, and both T1 and T2 phases programmed for each scanning condition. Three clinical surgeons evaluated the quality of the images via a standardized scale. Moreover, to analyze deformation of the two-dimensional image, the nerve diameter and total area of the micro-MRI images were compared after hematoxylin-eosin staining. The results show that rapid micro-MRI imaging method can be used for three-dimensional reconstruction of the fascicle structure. Nerve sample immersed in contrast agent（Mannerist Solution/GD-DTPA） and scanned in the T1 phase was the best. Moreover, the nerve sample was scanned freshly and can be recycled for other procedures. MRI images show better stability and smaller deformation compared with histological images. In conclusion, micro-MRI provides a feasible and rapid method for three-dimensional reconstruction of peripheral nerve fascicles, which can clearly show the internal structure of the peripheral nerve.展开更多
目的:分析自发性脊髓脑脊液漏所致自发性颅内低压(SIH)的临床特点。方法:对42例保守治疗无效的自发性脊髓脑脊液漏所致SIH患者的临床资料和影像学表现进行回顾性分析,并按病程长短分为短病程组(n=27)和长病程组(n=15),比较两组患者的临...目的:分析自发性脊髓脑脊液漏所致自发性颅内低压(SIH)的临床特点。方法:对42例保守治疗无效的自发性脊髓脑脊液漏所致SIH患者的临床资料和影像学表现进行回顾性分析,并按病程长短分为短病程组(n=27)和长病程组(n=15),比较两组患者的临床表现和影像学特征。结果:直立性头痛是SIH患者最典型的临床表现,出现率92.9%(39/42);在长病程组中头痛的发生比例明显低于短病程组(80%与100%,P=0.040)。与短病程组比较,长病程组的腰椎穿刺压力≥60.0 mm H2O的患者数明显增加(60.0%与20.8%,P=0.019),平均腰椎穿刺压力增高[(64.7±42.1)mm H2O与(40.0±33.8)mm H2O,P=0.038)],硬膜下出血的发生率升高(50.0%与11.6%,P=0.018)。所有患者接受CT脊髓造影检查均发现脑脊液漏点,38例为多发漏点,4例仅表现为单个漏点。结论:SIH的临床表现多样,随病程延长,其临床表现和腰椎穿刺压力可有部分改善,影像学检查如颅脑MRI、CT脊髓造影能帮助诊断;病程延长后硬膜下出血的发生概率增加,需积极干预。展开更多
基金supported by grants from the National Key Research and Development Plan of China,No.31670986(to QTZ)the Science and Technology Project of Guangdong Province of China,No.2014B020227001,2017A050501017(to QTZ)the Science and Technology Project of Guangzhou of China,No.201807010082(to QTZ),201704030041(to JQ)
文摘The most common methods for three-dimensional reconstruction of peripheral nerve fascicles include histological and radiology techniques. Histological techniques have many drawbacks including an enormous manual workload and poor image registration. Micro-magnetic resonance imaging（Micro-MRI）, an emerging radiology technique, has been used to report results in the brain, liver and tumor tissues. However, micro-MRI usage for obtaining intraneural structures has not been reported. The aim of this study was to present a new imaging method for three-dimensional reconstruction of peripheral nerve fascicles by ~1T micro-MRI. Freshly harvested sciatic nerve samples from an amputated limb were divided into four groups. Two different scanning conditions（Mannerist Solution/GD-DTPA contrast agent, distilled water） were selected, and both T1 and T2 phases programmed for each scanning condition. Three clinical surgeons evaluated the quality of the images via a standardized scale. Moreover, to analyze deformation of the two-dimensional image, the nerve diameter and total area of the micro-MRI images were compared after hematoxylin-eosin staining. The results show that rapid micro-MRI imaging method can be used for three-dimensional reconstruction of the fascicle structure. Nerve sample immersed in contrast agent（Mannerist Solution/GD-DTPA） and scanned in the T1 phase was the best. Moreover, the nerve sample was scanned freshly and can be recycled for other procedures. MRI images show better stability and smaller deformation compared with histological images. In conclusion, micro-MRI provides a feasible and rapid method for three-dimensional reconstruction of peripheral nerve fascicles, which can clearly show the internal structure of the peripheral nerve.
文摘目的:分析自发性脊髓脑脊液漏所致自发性颅内低压(SIH)的临床特点。方法:对42例保守治疗无效的自发性脊髓脑脊液漏所致SIH患者的临床资料和影像学表现进行回顾性分析,并按病程长短分为短病程组(n=27)和长病程组(n=15),比较两组患者的临床表现和影像学特征。结果:直立性头痛是SIH患者最典型的临床表现,出现率92.9%(39/42);在长病程组中头痛的发生比例明显低于短病程组(80%与100%,P=0.040)。与短病程组比较,长病程组的腰椎穿刺压力≥60.0 mm H2O的患者数明显增加(60.0%与20.8%,P=0.019),平均腰椎穿刺压力增高[(64.7±42.1)mm H2O与(40.0±33.8)mm H2O,P=0.038)],硬膜下出血的发生率升高(50.0%与11.6%,P=0.018)。所有患者接受CT脊髓造影检查均发现脑脊液漏点,38例为多发漏点,4例仅表现为单个漏点。结论:SIH的临床表现多样,随病程延长,其临床表现和腰椎穿刺压力可有部分改善,影像学检查如颅脑MRI、CT脊髓造影能帮助诊断;病程延长后硬膜下出血的发生概率增加,需积极干预。
