Knowledge of oxygen consumption rates and asphyxiation points in fish is important to determine appropriate stocking and water quality management in aquaculture. The oxygen consumption rate and asphyxiation point in C...Knowledge of oxygen consumption rates and asphyxiation points in fish is important to determine appropriate stocking and water quality management in aquaculture. The oxygen consumption rate and asphyxiation point in Chanodichthys mongolicus were detected under laboratory conditions using an improved respirometer chamber. The results revealed that more accurate estimates can be obtained by adjusting the volume of the respirometer chamber, which may avoid system errors caused by either repeatedly adjusting fish density or selecting different equipment specifications. The oxygen consumption rate and asphyxiation point of C. mongolicus increased with increasing water temperature and decreasing fish size. Changes in the C. mongolicus oxygen consumption rate were divided into three stages at water temperatures of 11-33~C： （I） a low temperature oxygen consumption rate stage when water temperature was 11-19~C, （2） the optimum temperature oxygen consumption rate stage when water temperature was 19-23~C, and （3） a high temperature oxygen consumption rate stage when water temperature was 〉27~C. The temperature quotients （Q10） obtained suggested that C. mongolicus preferred a temperature range of 19-23~C. At 19~C, C. mongolicus exhibited higher oxygen consumption rates during the day when the maximum values were observed at 10：00 and 14：00 than at night when the minimum occurred at 02：00.展开更多
In order to investigate detonation propagation and distribution problems of premixed CH_4 ＋ 2O_2 mixture around a concrete structure such as a refuge chamber,detonation experiments in one small size tube were conduct...In order to investigate detonation propagation and distribution problems of premixed CH_4 ＋ 2O_2 mixture around a concrete structure such as a refuge chamber,detonation experiments in one small size tube were conducted. A simulation method was developed to obtain the flow field load distribution in the coal mine lane and pressure load of each part for the refuge chamber. Firstly,a physical model of a full-size explosiontest lane was established,which included the refuge chamber. With the calculations of the exact initial detonation pressure,the propagation characteristics of CH_4 ＋ 2O_2 premixed mixture detonation in the lane was simulated. Simulation results of various parts from AUTODYN are recorded,and the shock wave arrival time and the pressure peak can be observed from the detonation pressure-time curve over the changing propagation distance. So curve differences in different locations cannot be ignored. Then by detonation experiments in the small size tube,detonation pressure-time curves and velocity were obtained. Finally the simulation waveform of variation curve agreed well with the experimental results,which validated the detonation simulation method. The difference between shockwaves of the two sensors confirmed that detonation wave changed along with distance and time. These results should be taken into serious consideration for the detonation progration and distribution problem in future researches.展开更多
AIM To assess whether higher sensitivity of colonic epithelium to hypoxia at the serosal side is associated with oxygen transfer asymmetry.METHODS Rats were fed either with normal chow or a lowsodium diet. Tissues wer...AIM To assess whether higher sensitivity of colonic epithelium to hypoxia at the serosal side is associated with oxygen transfer asymmetry.METHODS Rats were fed either with normal chow or a lowsodium diet. Tissues were mounted as flat sheets in a modified, airtight Ussing chamber with oxygen meters in each hemichamber. Mucosal samples from normal diet animals were studied under control conditions, in low-chloride solution and after adding chloride secretion inhibitors and chloride secretagogues. Samples from sodium-deprived rats were studied before and after ouabain addition. In separate experiments, the correlation between short-circuit current and oxygen consumption was analyzed. Finally, hypoxia was induced in one hemichamber to assess the relationship between its oxygen content and the oxygen pressure differencebetween both hemichambers. RESULTS In all studied conditions, oxygen consumption was larger in the serosal hemichamber than in the mucosal one(P = 0.0025 to P < 0.0001). Short-circuit current showed significant correlation with both total oxygen consumption(r = 0.765; P = 0.009) in normoxia and oxygen consumption in the serosal hemichamber(r = 0.754; P = 0.011) during mucosal hypoxia, but not with oxygen consumption in the mucosal hemichamber. When hypoxia was induced in the mucosal hemichamber, an oxygen pressure difference of 13 k Pa with the serosal hemichamber was enough to keep its oxygen content constant. However, when hypoxia was induced in the serosal hemichamber, the oxygen pressure difference with the mucosal hemichamber necessary to keep its oxygen content constant was 40 k Pa(P < 0.0001). CONCLUSION Serosal oxygen supply is more readily available to support short-circuit current. This may be partly due to a rectifying behavior of transepithelial oxygen transfer.展开更多
The Hyperbaric and Diving Medicine Centre of Singapore provides regular treatment for various UHMS indications for hyperbaric treatment in our dual multi-place connected chambers. When any single patient requires evac...The Hyperbaric and Diving Medicine Centre of Singapore provides regular treatment for various UHMS indications for hyperbaric treatment in our dual multi-place connected chambers. When any single patient requires evacuation during the treatment, a connected dual chamber facility is advantageous to avoid disruption for the remaining patients. We share our experience and procedures to facilitate repeated unexpected evacuation with minimal disruption to other patients undergoing treatment, in an elderly semiactivity dependent patient who had gastrointestinal symptoms during treatment.展开更多
为实现燃烧室组件的精确建模及其动力学特性的仿真研究,以零维时滞燃烧室模型为基础,考虑燃烧室内喷射、雾化、蒸发、混合、化学反应过程,采用针栓喷注器SMD(Sauter Mean Diameter,索特尔平均直径)经验关联式以及液滴高压蒸发理论对液氧...为实现燃烧室组件的精确建模及其动力学特性的仿真研究,以零维时滞燃烧室模型为基础,考虑燃烧室内喷射、雾化、蒸发、混合、化学反应过程,采用针栓喷注器SMD(Sauter Mean Diameter,索特尔平均直径)经验关联式以及液滴高压蒸发理论对液氧/甲烷推进剂组合的燃烧时滞进行求解,建立了基于液滴高压蒸发理论的变时滞燃烧室模型。基于1 kg/s级推力室开展热试车验证了变时滞燃烧室模型的准确性,结果表明:所建立的变时滞燃烧室模型可以较为准确地预测燃烧室的压力以及温度动态响应过程,与试验结果相比,稳态压力以及温度误差均在6%以内,压力参数动态响应时间的误差在14%以内,仿真结果具有较高的精度。基于变时滞燃烧室模型开展仿真研究,研究发现:液氧液滴初始粒径以及燃烧室温度作为影响液氧液滴寿命的主要因素,主导着液氧时滞的变化;变时滞模型可以根据工况参数动态计算推进剂燃烧时滞,启动初期喷注器雾化效果较差,液滴最大粒径达到800μm,且燃烧室温度低,进而导致燃烧时滞偏大,最大达到了1100 ms,约为稳定工作状态下燃烧时滞的40倍。本文所建立的变时滞燃烧室模型可根据工况参数对燃烧时滞进行动态计算,相较于传统时滞模型,其燃烧时滞的变化趋势更符合发动机实际工作过程,同时其室压的响应时间、稳态值也更接近实验值,该模型未来可为实际发动机时序设计等提供仿真支撑。展开更多
Background:Hypoxia is a significant risk factor of hypertension.However,no studies have used transcutaneous tissue partial pressure of oxygen(TcPO_(2))and partial pressure of carbon dioxide(TcPCO_(2))monitors to measu...Background:Hypoxia is a significant risk factor of hypertension.However,no studies have used transcutaneous tissue partial pressure of oxygen(TcPO_(2))and partial pressure of carbon dioxide(TcPCO_(2))monitors to measure the respective partial pressures in healthy individuals.Oxygen saturation(SpO_(2))is often used for traditional monitoring of vital signs.This study investigated the changes in TcPO_(2)and SpO_(2)values during rapid changes in altitude.The trial was registered at ClinicalTrials.gov(registration no.NCT06076057).Methods:Healthy adult volunteers were instructed to sit vertically in a hypobaric oxygen chamber,which ascended from 0 m to 2500 m at a uniform speed within 10 min.The Danish Radiometer TCM4 was used to measure TcPO_(2)and TcPCO_(2)with the ventral side of the upper arm as the measurement site.The Shenzhen Kerokan P0D-1 W pulse oximeter was used to measure heart rate and SpO_(2),with values recorded once every 500 m.Results:Altogether,49 healthy volunteers were recruited between March 2023 and August 2023.With increasing altitude,TcPO_(2)and SpO_(2)decreased significantly(P<0.01).During the ascent from 0 m,TcPO_(2)began to change statistically at 500 m(P<0.05),whereas SpO_(2)began to change statistically at 1000 m(P<0.05).At the same altitude,the difference in TcPO_(2)was greater than the difference in SpO_(2).At 1000 m,there were statistically significant changes in TcPO_(2)and SpO_(2)(P<0.001).At altitudes>500 m,statistical significance was identified between TcPO_(2)in both sexes(P<0.05).Statistical significance in TcPCO_(2)and heart rate was observed at the different elevations(P<0.05).Conclusion:In acutely changing low-pressure hypoxic environments,TcPO_(2)changed more dramatically than SpO_(2).展开更多
基金Supported by the Central-Level Non-Profit Scientific Research Institutes Special Funds(Nos.2014A07XK04,HSY201403)the Harbin Science and Technology Project(No.2012AA6CN037)the National Key Technology Research and Development Program of China(No.2012BAD25B09)
文摘Knowledge of oxygen consumption rates and asphyxiation points in fish is important to determine appropriate stocking and water quality management in aquaculture. The oxygen consumption rate and asphyxiation point in Chanodichthys mongolicus were detected under laboratory conditions using an improved respirometer chamber. The results revealed that more accurate estimates can be obtained by adjusting the volume of the respirometer chamber, which may avoid system errors caused by either repeatedly adjusting fish density or selecting different equipment specifications. The oxygen consumption rate and asphyxiation point of C. mongolicus increased with increasing water temperature and decreasing fish size. Changes in the C. mongolicus oxygen consumption rate were divided into three stages at water temperatures of 11-33~C： （I） a low temperature oxygen consumption rate stage when water temperature was 11-19~C, （2） the optimum temperature oxygen consumption rate stage when water temperature was 19-23~C, and （3） a high temperature oxygen consumption rate stage when water temperature was 〉27~C. The temperature quotients （Q10） obtained suggested that C. mongolicus preferred a temperature range of 19-23~C. At 19~C, C. mongolicus exhibited higher oxygen consumption rates during the day when the maximum values were observed at 10：00 and 14：00 than at night when the minimum occurred at 02：00.
基金Supported by the National Science Foundation of China(E041003)the Fundamental Research Funds for the Central Universities(FRF-TP-15-105 A1)the Postdoctoral Science Foundation of China(2015M580049)
文摘In order to investigate detonation propagation and distribution problems of premixed CH_4 ＋ 2O_2 mixture around a concrete structure such as a refuge chamber,detonation experiments in one small size tube were conducted. A simulation method was developed to obtain the flow field load distribution in the coal mine lane and pressure load of each part for the refuge chamber. Firstly,a physical model of a full-size explosiontest lane was established,which included the refuge chamber. With the calculations of the exact initial detonation pressure,the propagation characteristics of CH_4 ＋ 2O_2 premixed mixture detonation in the lane was simulated. Simulation results of various parts from AUTODYN are recorded,and the shock wave arrival time and the pressure peak can be observed from the detonation pressure-time curve over the changing propagation distance. So curve differences in different locations cannot be ignored. Then by detonation experiments in the small size tube,detonation pressure-time curves and velocity were obtained. Finally the simulation waveform of variation curve agreed well with the experimental results,which validated the detonation simulation method. The difference between shockwaves of the two sensors confirmed that detonation wave changed along with distance and time. These results should be taken into serious consideration for the detonation progration and distribution problem in future researches.
基金Supported by The Secretaría de Ciencia,Técnica y Posgrado,National University of Cuyo,Mendoza 5500 Argentina to Saraví FD,No.06/J457
文摘AIM To assess whether higher sensitivity of colonic epithelium to hypoxia at the serosal side is associated with oxygen transfer asymmetry.METHODS Rats were fed either with normal chow or a lowsodium diet. Tissues were mounted as flat sheets in a modified, airtight Ussing chamber with oxygen meters in each hemichamber. Mucosal samples from normal diet animals were studied under control conditions, in low-chloride solution and after adding chloride secretion inhibitors and chloride secretagogues. Samples from sodium-deprived rats were studied before and after ouabain addition. In separate experiments, the correlation between short-circuit current and oxygen consumption was analyzed. Finally, hypoxia was induced in one hemichamber to assess the relationship between its oxygen content and the oxygen pressure differencebetween both hemichambers. RESULTS In all studied conditions, oxygen consumption was larger in the serosal hemichamber than in the mucosal one(P = 0.0025 to P < 0.0001). Short-circuit current showed significant correlation with both total oxygen consumption(r = 0.765; P = 0.009) in normoxia and oxygen consumption in the serosal hemichamber(r = 0.754; P = 0.011) during mucosal hypoxia, but not with oxygen consumption in the mucosal hemichamber. When hypoxia was induced in the mucosal hemichamber, an oxygen pressure difference of 13 k Pa with the serosal hemichamber was enough to keep its oxygen content constant. However, when hypoxia was induced in the serosal hemichamber, the oxygen pressure difference with the mucosal hemichamber necessary to keep its oxygen content constant was 40 k Pa(P < 0.0001). CONCLUSION Serosal oxygen supply is more readily available to support short-circuit current. This may be partly due to a rectifying behavior of transepithelial oxygen transfer.
文摘The Hyperbaric and Diving Medicine Centre of Singapore provides regular treatment for various UHMS indications for hyperbaric treatment in our dual multi-place connected chambers. When any single patient requires evacuation during the treatment, a connected dual chamber facility is advantageous to avoid disruption for the remaining patients. We share our experience and procedures to facilitate repeated unexpected evacuation with minimal disruption to other patients undergoing treatment, in an elderly semiactivity dependent patient who had gastrointestinal symptoms during treatment.
文摘为实现燃烧室组件的精确建模及其动力学特性的仿真研究,以零维时滞燃烧室模型为基础,考虑燃烧室内喷射、雾化、蒸发、混合、化学反应过程,采用针栓喷注器SMD(Sauter Mean Diameter,索特尔平均直径)经验关联式以及液滴高压蒸发理论对液氧/甲烷推进剂组合的燃烧时滞进行求解,建立了基于液滴高压蒸发理论的变时滞燃烧室模型。基于1 kg/s级推力室开展热试车验证了变时滞燃烧室模型的准确性,结果表明:所建立的变时滞燃烧室模型可以较为准确地预测燃烧室的压力以及温度动态响应过程,与试验结果相比,稳态压力以及温度误差均在6%以内,压力参数动态响应时间的误差在14%以内,仿真结果具有较高的精度。基于变时滞燃烧室模型开展仿真研究,研究发现:液氧液滴初始粒径以及燃烧室温度作为影响液氧液滴寿命的主要因素,主导着液氧时滞的变化;变时滞模型可以根据工况参数动态计算推进剂燃烧时滞,启动初期喷注器雾化效果较差,液滴最大粒径达到800μm,且燃烧室温度低,进而导致燃烧时滞偏大,最大达到了1100 ms,约为稳定工作状态下燃烧时滞的40倍。本文所建立的变时滞燃烧室模型可根据工况参数对燃烧时滞进行动态计算,相较于传统时滞模型,其燃烧时滞的变化趋势更符合发动机实际工作过程,同时其室压的响应时间、稳态值也更接近实验值,该模型未来可为实际发动机时序设计等提供仿真支撑。
基金supported by grants from Shandong Province Key R&D Program(2021CXGC011301).
文摘Background:Hypoxia is a significant risk factor of hypertension.However,no studies have used transcutaneous tissue partial pressure of oxygen(TcPO_(2))and partial pressure of carbon dioxide(TcPCO_(2))monitors to measure the respective partial pressures in healthy individuals.Oxygen saturation(SpO_(2))is often used for traditional monitoring of vital signs.This study investigated the changes in TcPO_(2)and SpO_(2)values during rapid changes in altitude.The trial was registered at ClinicalTrials.gov(registration no.NCT06076057).Methods:Healthy adult volunteers were instructed to sit vertically in a hypobaric oxygen chamber,which ascended from 0 m to 2500 m at a uniform speed within 10 min.The Danish Radiometer TCM4 was used to measure TcPO_(2)and TcPCO_(2)with the ventral side of the upper arm as the measurement site.The Shenzhen Kerokan P0D-1 W pulse oximeter was used to measure heart rate and SpO_(2),with values recorded once every 500 m.Results:Altogether,49 healthy volunteers were recruited between March 2023 and August 2023.With increasing altitude,TcPO_(2)and SpO_(2)decreased significantly(P<0.01).During the ascent from 0 m,TcPO_(2)began to change statistically at 500 m(P<0.05),whereas SpO_(2)began to change statistically at 1000 m(P<0.05).At the same altitude,the difference in TcPO_(2)was greater than the difference in SpO_(2).At 1000 m,there were statistically significant changes in TcPO_(2)and SpO_(2)(P<0.001).At altitudes>500 m,statistical significance was identified between TcPO_(2)in both sexes(P<0.05).Statistical significance in TcPCO_(2)and heart rate was observed at the different elevations(P<0.05).Conclusion:In acutely changing low-pressure hypoxic environments,TcPO_(2)changed more dramatically than SpO_(2).
