An increasing data indicates that altered microRNAs(miRNAs)participate in the radiation-induced DNA damage response.However,a correlation of mRNA and miRNA profiles across the entire genome and in response to irradiat...An increasing data indicates that altered microRNAs(miRNAs)participate in the radiation-induced DNA damage response.However,a correlation of mRNA and miRNA profiles across the entire genome and in response to irradiation has not been thoroughly assessed.We analyzed miRNA microarray data collected from HeLa cells after ionizing radiation(IR),quantified the expression profiles of mRNAs and performed comparative analysis of the data sets using target prediction algorithms,Gene Ontology(GO)analysis,pathway analysis,and gene network construction.The results showed that the altered miRNAs were involved in regulation of various cellular functions.miRNA-gene network analyses revealed that miR-186,miR-106b,miR-15a/b,CCND1and CDK6 played vital role in the cellular radiation response.Using qRT-PCR,we confirmed that twenty-two miRNAs showed differential expression in HeLa cells treated with IR and some of these miRNAs affected cell cycle progression.This study demonstrated that miRNAs influence gene expression in the entire genome during the cellular radiation response and suggested vital pathways for further research.展开更多
Ionizing radiation can cause radiation injury to the human body under certain circumstances,such as unexpected radiation accidents and nuclear terrorist attacks.Methods that can accurately and rapidly measure the biol...Ionizing radiation can cause radiation injury to the human body under certain circumstances,such as unexpected radiation accidents and nuclear terrorist attacks.Methods that can accurately and rapidly measure the biological effects are necessary for rational triage and treatment of affected patients.In recent decades,significant researches attention has been focused on the development of accurate and efficient biodosimeters.In this review,we summarize recent developments in biological dosimeters,with a primary focus on cell-based and molecular markers,including comet assay,dicentric chromosome aberration,γ-H2AX,micronuclei,microRNA,lncRNA,and 8-Oxo-dG.展开更多
Non-coding RNAs(ncRNAs)have attracted wide attention in the field of ionizing radiation(IR).They have been found to be involved in cell development,proliferation,differentiation,and apoptosis in both clinical radiothe...Non-coding RNAs(ncRNAs)have attracted wide attention in the field of ionizing radiation(IR).They have been found to be involved in cell development,proliferation,differentiation,and apoptosis in both clinical radiotherapy and radiation protection studies.Despite the production of an increasing number of relevant reports in recent years,the molecular mechanisms involved are yet to be clearly understood.In the last few years,ncRNAs have received a lot of research attention,especially microRNAs,long noncoding RNAs,and circular RNAs,which have a well-known association with the development of human disease and are closely related to the biological phenotypic changes caused by IR.With recent developments of research methods,these ncRNAs might play a significant regulatory role in future radiation protection and radiotherapy.This review will summarize the research progress of various types of ncRNAs as biomarkers of IR and potential new targets for radiotherapy,aiming to provide references for future research and the formulation of new strategies.展开更多
Objective miR-663 a has been reported to be downregulated by X-ray irradiation and participates in radiation-induced bystander effect via TGF-β1.The goal of this study was to explore the role of mi R-663 a during rad...Objective miR-663 a has been reported to be downregulated by X-ray irradiation and participates in radiation-induced bystander effect via TGF-β1.The goal of this study was to explore the role of mi R-663 a during radiation-induced Epithelium-to-mesenchymal transition(EMT).Methods TGF-β1 or IR was used to induce EMT.After mi R-663 a transfection,cell migration and cell morphological changes were detected and the expression levels of mi R-663 a,TGF-β1,and EMT-related factors were quantified.Results Enhancement of cell migration and promotion of mesenchymal changes induced by either TGF-β1 or radiation were suppressed by mi R-663 a.Furthermore,both X-ray and carbon ion irradiation resulted in the upregulation of TGF-β1 and downregulation of mi R-663 a,while the silencing of TGF-β1 by mi R-663 a reversed the EMT process after radiation.Conclusion Our findings demonstrate an EMT-suppressing effect by mi R-663 a via TGF-β1 in radiationinduced EMT.展开更多
目的探讨低剂量电离辐射引起DNA拓扑异构酶IIα(TOPO IIα)蛋白质和mRNA表达变化规律。方法用W estern b lot和RT-PCR的方法分别检测Hela细胞在接受低剂量电离辐射后不同时间、不同剂量和多次辐射的累积后蛋白质和mRNA的变化。结果细胞...目的探讨低剂量电离辐射引起DNA拓扑异构酶IIα(TOPO IIα)蛋白质和mRNA表达变化规律。方法用W estern b lot和RT-PCR的方法分别检测Hela细胞在接受低剂量电离辐射后不同时间、不同剂量和多次辐射的累积后蛋白质和mRNA的变化。结果细胞受5Gy的照射时,TOPOⅡα蛋白的表达较对照在4h已开始下降,12h降为最低,16h已开始恢复,mRNA在4h开始下降,8h降为最低,12h开始恢复,较蛋白的变化早;细胞受不同剂量照射后12h,TOPOⅡα蛋白和mRNA的表达均较对照降低,且下降程度随剂量增加而增加,且具有次数累加效应。结论低剂量电离辐射引起TOPO IIα表达的变化具时间效应、剂量效应和多次累加效应,mRNA的变化早于蛋白质的变化。展开更多
基金supported by the National Basic Research Program of China(2010CB912801 and 2007CB914601)the National Natural Science Foundation of China(31170713)the Beijing Municipal Natural Science Foundation(5112029)
文摘An increasing data indicates that altered microRNAs(miRNAs)participate in the radiation-induced DNA damage response.However,a correlation of mRNA and miRNA profiles across the entire genome and in response to irradiation has not been thoroughly assessed.We analyzed miRNA microarray data collected from HeLa cells after ionizing radiation(IR),quantified the expression profiles of mRNAs and performed comparative analysis of the data sets using target prediction algorithms,Gene Ontology(GO)analysis,pathway analysis,and gene network construction.The results showed that the altered miRNAs were involved in regulation of various cellular functions.miRNA-gene network analyses revealed that miR-186,miR-106b,miR-15a/b,CCND1and CDK6 played vital role in the cellular radiation response.Using qRT-PCR,we confirmed that twenty-two miRNAs showed differential expression in HeLa cells treated with IR and some of these miRNAs affected cell cycle progression.This study demonstrated that miRNAs influence gene expression in the entire genome during the cellular radiation response and suggested vital pathways for further research.
基金This review was funded by Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2017-1001-08)National Natural Science Foundation of China(81772243,31971168,81803172).
文摘Ionizing radiation can cause radiation injury to the human body under certain circumstances,such as unexpected radiation accidents and nuclear terrorist attacks.Methods that can accurately and rapidly measure the biological effects are necessary for rational triage and treatment of affected patients.In recent decades,significant researches attention has been focused on the development of accurate and efficient biodosimeters.In this review,we summarize recent developments in biological dosimeters,with a primary focus on cell-based and molecular markers,including comet assay,dicentric chromosome aberration,γ-H2AX,micronuclei,microRNA,lncRNA,and 8-Oxo-dG.
基金the Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions,the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)China,and the Nuclear Energy Development Project,China(No.2016-1295).
文摘Non-coding RNAs(ncRNAs)have attracted wide attention in the field of ionizing radiation(IR).They have been found to be involved in cell development,proliferation,differentiation,and apoptosis in both clinical radiotherapy and radiation protection studies.Despite the production of an increasing number of relevant reports in recent years,the molecular mechanisms involved are yet to be clearly understood.In the last few years,ncRNAs have received a lot of research attention,especially microRNAs,long noncoding RNAs,and circular RNAs,which have a well-known association with the development of human disease and are closely related to the biological phenotypic changes caused by IR.With recent developments of research methods,these ncRNAs might play a significant regulatory role in future radiation protection and radiotherapy.This review will summarize the research progress of various types of ncRNAs as biomarkers of IR and potential new targets for radiotherapy,aiming to provide references for future research and the formulation of new strategies.
基金funded by the National Natural Science Foundation of China[no.U1932208,YANG Kai]the Science and Technology Research Project of Gansu Province[no.17JR5RA307 and 145RTSA012,WANG Ju Fang]the Science and Technology Research Project of Gansu Province[no.21JR7RA108,DING Nan]
文摘Objective miR-663 a has been reported to be downregulated by X-ray irradiation and participates in radiation-induced bystander effect via TGF-β1.The goal of this study was to explore the role of mi R-663 a during radiation-induced Epithelium-to-mesenchymal transition(EMT).Methods TGF-β1 or IR was used to induce EMT.After mi R-663 a transfection,cell migration and cell morphological changes were detected and the expression levels of mi R-663 a,TGF-β1,and EMT-related factors were quantified.Results Enhancement of cell migration and promotion of mesenchymal changes induced by either TGF-β1 or radiation were suppressed by mi R-663 a.Furthermore,both X-ray and carbon ion irradiation resulted in the upregulation of TGF-β1 and downregulation of mi R-663 a,while the silencing of TGF-β1 by mi R-663 a reversed the EMT process after radiation.Conclusion Our findings demonstrate an EMT-suppressing effect by mi R-663 a via TGF-β1 in radiationinduced EMT.
