[Objective] To screen out a microbial flocculant with good decolorization effect on methyl orange wastewater,and study the effect of different cultural conditions on decolorization effect of methyl orange.[Method] Abs...[Objective] To screen out a microbial flocculant with good decolorization effect on methyl orange wastewater,and study the effect of different cultural conditions on decolorization effect of methyl orange.[Method] Absorbance of methyl orange solution before and after decolorization was determined by spectrophotometer,and the decolorization rate was calculated to compare the effects of different cultural conditions on removal rate of methyl orange.[Result]An optimal actinomycete stain（F-1-2） was screened out,and the best cultural condition was as follows：with sucrose as carbon source and NaNO3 as nitrogen source,cultured in constant temperature oscillator at 150 r/min,30℃ for 72 h.Under the optimal condition,the removal rate against methyl orange could reach 68.4%.[Conclusion]Different culture conditions have great impact on decolorization effect of strain.展开更多
A new kind of compounded microbial flocculant (CMBF) for water and wastewater treatment has been developed through biological technology. In order to discuss its biological security, four groups of experiments, rat ac...A new kind of compounded microbial flocculant (CMBF) for water and wastewater treatment has been developed through biological technology. In order to discuss its biological security, four groups of experiments, rat acute toxicity test via mouth, salmonella assay in vitro, mouse micronucleus in vivo test and teratogenesis test were conducted to evaluate its general toxicity, genotoxicity and generative toxicity. The experimental results showed that this type of compounded microbial flocculant was a substantial non-toxic substance based on the fact that LD50 value was over 10 mg/kg. The results from salmonella in vivo test and mouse micronucleus in vivo test revealed that the compounded microbial flocculant is a genetically non-toxic substance. Furthermore, compounded microbial flocculant has little effect on the growth of all the rats, and any morphologic abnormal phenomenon hasn’t appeared.展开更多
The flocculation effect of the Phanerochaete chrysosporium on the coal slurry were studied by orthogonal experimental method in this study.The results of research show that the P.chrysosporium has a good effect on flo...The flocculation effect of the Phanerochaete chrysosporium on the coal slurry were studied by orthogonal experimental method in this study.The results of research show that the P.chrysosporium has a good effect on flocculating coal slurry.The optimum combination of the experimental is the P.chrysosporium,which is cultured in 2 days,mixed with 2 mL coagulant and 2 mL broth.The flocculant is a broken liquid with pH value of 6.The hightest flocculation ratio is 93.5%.The result of FTIR shows that the biological extraction of P.chrysosporium contains a lot of acidic polysaccharides that has the effect on flocculation.Microbial flocculant molecules and particles of coal slurry could be flocculated by‘‘absorption bridge’’.展开更多
Microbial flocculant （MBF） is a kind of novel flocculant, which is widely used for the food industry, highly concentrated organic waste water treatment and the urban water supply technology. Due to its characteristi...Microbial flocculant （MBF） is a kind of novel flocculant, which is widely used for the food industry, highly concentrated organic waste water treatment and the urban water supply technology. Due to its characteristics of superior flocculation effect, without secondary pollution to the environment and being easily degraded, MBF has been emerging as a hotspot of water treatment research in recent years. In this paper, the latest progress of the preparation of MBFs and the flocculating mechanism were presented. The practical application research of MBFs for water treatment was reviewed. Finally, suggestions and development prospect of research on MBFs were discussed.展开更多
This paper dealt with non-parallelism between the effect of microbial flocculant (MBF) on sewerage disposal and the flocculation rate, the high flocculation rate doesn’t mean the fine disposal effectiveness, and reve...This paper dealt with non-parallelism between the effect of microbial flocculant (MBF) on sewerage disposal and the flocculation rate, the high flocculation rate doesn’t mean the fine disposal effectiveness, and revealed the problem encountered when the exclusive parameter―flocculation rate is used to evaluate the effect of MBF on sewerage disposal. The results showed that MBF made from different carbon sources have some influence on the effectiveness of sewerage disposal; flocculation rate cannot authentically reflect the status of sewerage disposal. When the exclusive parameter―flocculation rate is used to evaluate the effect of MBF on sewerage disposal, it would be exaggerated, especially in disposing COD-low sewerage. The authors considered that when flocculation rate is used to evaluate the effect of MBF on sewerage disposal, it is better to take COD-removal rate into account.展开更多
A high effective bioflocculant-producing strain named LB1 was isolated and screened from the leachate by routine micro- method. The strain was identified as genus Pseudomonas according to the morphologic and physiolog...A high effective bioflocculant-producing strain named LB1 was isolated and screened from the leachate by routine micro- method. The strain was identified as genus Pseudomonas according to the morphologic and physiological-biochemical characteristics of the strain. The biological characteristics of the microbial flocculants produced by LB1 were investigated. The results show that the optimal production period of microbial flocculant using LB1 is 96 h; and the products, found in the fermentation liquor, comprise the extracellular organic matter （EOM） of LB1 generated during LB1 growth and the secondary metabolites in the anaphase of LB1. Meanwhile, the mycelia can improve the performance of the microbial flocculants. Because heat has a greater influence on the active substance in the microbial flocculants than other factors, the activity of the flocculants decreases with increasing temperature and the time of heating. The flocculants can flocculate mud-containing and melanin-containing wastewater effectively with a flocculation ratio of 85.1% and 92.2%, respectively. The optimal heating temperature varies from 20 to 45 ℃. When flocculating the two wastewater, the flocculating activity of the flocculants is above 57.7% and 70.9%, respectively, in a wide pH range from 3 to 11.展开更多
[Objective] The purpose was to select out and identify a flocculant producing strain which could produce high active flocculant.[Method] The strain producing high active flocculant was isolated out and purified throug...[Objective] The purpose was to select out and identify a flocculant producing strain which could produce high active flocculant.[Method] The strain producing high active flocculant was isolated out and purified through medium culture and the selected strain was identified through observing its culture characters and determining its physiological and biochemical property.[Result] Fourteen strains of bacteria with flocculant producing function were isolated from tested soil samples through isolation,purification and preliminary screening using dilution-spread plate method and plate streaking method.Five strains of flocculant producing bacteria showing higher flocculation activity were selected out after second screening and their flocculation rates were higher than 70%;the flocculation activity of one strain among them was still stable after multiple subculturings,its flocculation rate was always above 90% and it was marked as TS-1.TS-1 was encapsulated Gram-positive bacillus and there was no lipid in it,such as poly-β-hydroxybutyric acid.TS-1 was Bacillus amyloliquefaciens,so it was named Bacillus TS-1.[Conclusion] The strain selected out in this experiment could be used in the flocculation and biochemical treatment of wastewater from starch industry.展开更多
基金Supported by National Natural Science Foundation of China(51064011)Jiangxi Education Science " Twelfth Five-year" Planning Program (10YB335)Science Foundation for Youths of Jiangxi Educational Committee (GJJ09599)~~
文摘[Objective] To screen out a microbial flocculant with good decolorization effect on methyl orange wastewater,and study the effect of different cultural conditions on decolorization effect of methyl orange.[Method] Absorbance of methyl orange solution before and after decolorization was determined by spectrophotometer,and the decolorization rate was calculated to compare the effects of different cultural conditions on removal rate of methyl orange.[Result]An optimal actinomycete stain（F-1-2） was screened out,and the best cultural condition was as follows：with sucrose as carbon source and NaNO3 as nitrogen source,cultured in constant temperature oscillator at 150 r/min,30℃ for 72 h.Under the optimal condition,the removal rate against methyl orange could reach 68.4%.[Conclusion]Different culture conditions have great impact on decolorization effect of strain.
文摘A new kind of compounded microbial flocculant (CMBF) for water and wastewater treatment has been developed through biological technology. In order to discuss its biological security, four groups of experiments, rat acute toxicity test via mouth, salmonella assay in vitro, mouse micronucleus in vivo test and teratogenesis test were conducted to evaluate its general toxicity, genotoxicity and generative toxicity. The experimental results showed that this type of compounded microbial flocculant was a substantial non-toxic substance based on the fact that LD50 value was over 10 mg/kg. The results from salmonella in vivo test and mouse micronucleus in vivo test revealed that the compounded microbial flocculant is a genetically non-toxic substance. Furthermore, compounded microbial flocculant has little effect on the growth of all the rats, and any morphologic abnormal phenomenon hasn’t appeared.
基金fnancial support provided by the National Natural Science Foundation of China(No.51274012)for this work is gratefully acknowledged
文摘The flocculation effect of the Phanerochaete chrysosporium on the coal slurry were studied by orthogonal experimental method in this study.The results of research show that the P.chrysosporium has a good effect on flocculating coal slurry.The optimum combination of the experimental is the P.chrysosporium,which is cultured in 2 days,mixed with 2 mL coagulant and 2 mL broth.The flocculant is a broken liquid with pH value of 6.The hightest flocculation ratio is 93.5%.The result of FTIR shows that the biological extraction of P.chrysosporium contains a lot of acidic polysaccharides that has the effect on flocculation.Microbial flocculant molecules and particles of coal slurry could be flocculated by‘‘absorption bridge’’.
文摘Microbial flocculant （MBF） is a kind of novel flocculant, which is widely used for the food industry, highly concentrated organic waste water treatment and the urban water supply technology. Due to its characteristics of superior flocculation effect, without secondary pollution to the environment and being easily degraded, MBF has been emerging as a hotspot of water treatment research in recent years. In this paper, the latest progress of the preparation of MBFs and the flocculating mechanism were presented. The practical application research of MBFs for water treatment was reviewed. Finally, suggestions and development prospect of research on MBFs were discussed.
基金Granted by the Funds of Chinese Academy of Sciences for the HundredTalents Program and the National Natural Science Foundation of China(Grant No. NSFC40463001).
文摘This paper dealt with non-parallelism between the effect of microbial flocculant (MBF) on sewerage disposal and the flocculation rate, the high flocculation rate doesn’t mean the fine disposal effectiveness, and revealed the problem encountered when the exclusive parameter―flocculation rate is used to evaluate the effect of MBF on sewerage disposal. The results showed that MBF made from different carbon sources have some influence on the effectiveness of sewerage disposal; flocculation rate cannot authentically reflect the status of sewerage disposal. When the exclusive parameter―flocculation rate is used to evaluate the effect of MBF on sewerage disposal, it would be exaggerated, especially in disposing COD-low sewerage. The authors considered that when flocculation rate is used to evaluate the effect of MBF on sewerage disposal, it is better to take COD-removal rate into account.
基金Science and Technology Key Projects ofSichuan Province (No.2008SZ0008)Foundation of Southwest Jiaotong University (No.2007B10)
文摘A high effective bioflocculant-producing strain named LB1 was isolated and screened from the leachate by routine micro- method. The strain was identified as genus Pseudomonas according to the morphologic and physiological-biochemical characteristics of the strain. The biological characteristics of the microbial flocculants produced by LB1 were investigated. The results show that the optimal production period of microbial flocculant using LB1 is 96 h; and the products, found in the fermentation liquor, comprise the extracellular organic matter （EOM） of LB1 generated during LB1 growth and the secondary metabolites in the anaphase of LB1. Meanwhile, the mycelia can improve the performance of the microbial flocculants. Because heat has a greater influence on the active substance in the microbial flocculants than other factors, the activity of the flocculants decreases with increasing temperature and the time of heating. The flocculants can flocculate mud-containing and melanin-containing wastewater effectively with a flocculation ratio of 85.1% and 92.2%, respectively. The optimal heating temperature varies from 20 to 45 ℃. When flocculating the two wastewater, the flocculating activity of the flocculants is above 57.7% and 70.9%, respectively, in a wide pH range from 3 to 11.
基金Supproted by the Key Project of Chinese Ministry of Education(211189)~~
文摘[Objective] The purpose was to select out and identify a flocculant producing strain which could produce high active flocculant.[Method] The strain producing high active flocculant was isolated out and purified through medium culture and the selected strain was identified through observing its culture characters and determining its physiological and biochemical property.[Result] Fourteen strains of bacteria with flocculant producing function were isolated from tested soil samples through isolation,purification and preliminary screening using dilution-spread plate method and plate streaking method.Five strains of flocculant producing bacteria showing higher flocculation activity were selected out after second screening and their flocculation rates were higher than 70%;the flocculation activity of one strain among them was still stable after multiple subculturings,its flocculation rate was always above 90% and it was marked as TS-1.TS-1 was encapsulated Gram-positive bacillus and there was no lipid in it,such as poly-β-hydroxybutyric acid.TS-1 was Bacillus amyloliquefaciens,so it was named Bacillus TS-1.[Conclusion] The strain selected out in this experiment could be used in the flocculation and biochemical treatment of wastewater from starch industry.
