Factors including Mg^2＋, dNTP and primer that affected RAPD were studied using orthogonal experimental design, and at the same time, anneal temperature, extending time and cycles were also studied. Finally, PCR react...Factors including Mg^2＋, dNTP and primer that affected RAPD were studied using orthogonal experimental design, and at the same time, anneal temperature, extending time and cycles were also studied. Finally, PCR reaction system that is feasible for black soil microbial PCR amplification was determined, containing 7 ng DNA template, 20 pM random primers, 1.5 U Taq DNA polymerase, 3.0 mM MgCl2 and 0.2 mmol ·L^-1 dNTP, with procedure： 40 cycles for 3 min at 94 ℃ （temperature）, 40 s at 37 ℃ （annealing）, 1.5 min at 72 ℃ （extension）, a final elongation step at 72 ℃ for 7 min.展开更多
Peri-implantitis has been defined as an inflamematory condition involving dental implants, surrounding mucosa and bone, which lose supporting bone. Although high success rates for endosseous implants have been reporte...Peri-implantitis has been defined as an inflamematory condition involving dental implants, surrounding mucosa and bone, which lose supporting bone. Although high success rates for endosseous implants have been reported, failures occur, and some implants are lost or removed. At least 10% of the failures have been suggested to be the result of peri-implantitis. One of the major causes of the peri-implantitis is the bacterial colonization of implant surfaces but additional risk factors such as periodontitis, poor oral hygiene, tobacco consumption, prepost operative therapies and genetic susceptibility should be considered. In the present study a real-time PCR bases assay was designed to detect and quantify red complex species, then used to investigate 307 periodontal pocket samples from 127 periodontitis patients and 180 controls. Results demonstrated a significant higher prevalence of red complex species and increased amount of Porphyromonas gingivalis and Treponema denticolain periodontal pocket of periodontitis. Since a higher risk of peri-implantitis occurs in periodontally affected patients, detection and treatment of bacteria is a fundamental objective to ensure dental implant survival.展开更多
Bioevolution is still a main puzzle and attracts many scientists to research on it. Here I present that organisms have two impor- tant properties, definite structure and self-reproduction. Based on the number and conn...Bioevolution is still a main puzzle and attracts many scientists to research on it. Here I present that organisms have two impor- tant properties, definite structure and self-reproduction. Based on the number and connection of the structural units, organisms can be di- vided into three groups, unicellular, particellular and polycellular organisms. It can be called polycellular evolution that organisms evolve from unicellular, particellular to polycellular. Also it can be called diploid evolution that organisms evolve from haplobes to diplobes, two groups based on the reproductive differences. Some concepts like spore, zygote and embryos are redefined in the paper. Moreover, I present that embryos are the most important boundary of bioevolution and organisms can be divided into two evolutionary phases, the lower and the higher. The lower organisms, Kingdom Microbia (kingd. nov.), are inembryonate, which include Acytophyla (phyl. nov.), bacteria, protozoa, fungi and inembryonate algae. The higher organisms are embryonate and have two branches, Kingdom Plantae and Kingdom Animalia. Plantae are sessile and, autotrophic or sporogenic, which include higher plants and Nudembryophyta (phyl. nov.). Animalia are heterotrophic and, motile or gametogenic, which include all multicellular animals. The new system, which reflects the two important phases of bioevolu- tion and two branches of higher organisms, can really correct the problem of different kingdoms in different researches or by different re- searchers.展开更多
基金This Work was Supported by the National Natural Science Foundation of China (30230250, 30370819) Heilongjiang ProvinceScience Foundation (C0215)
文摘Factors including Mg^2＋, dNTP and primer that affected RAPD were studied using orthogonal experimental design, and at the same time, anneal temperature, extending time and cycles were also studied. Finally, PCR reaction system that is feasible for black soil microbial PCR amplification was determined, containing 7 ng DNA template, 20 pM random primers, 1.5 U Taq DNA polymerase, 3.0 mM MgCl2 and 0.2 mmol ·L^-1 dNTP, with procedure： 40 cycles for 3 min at 94 ℃ （temperature）, 40 s at 37 ℃ （annealing）, 1.5 min at 72 ℃ （extension）, a final elongation step at 72 ℃ for 7 min.
文摘Peri-implantitis has been defined as an inflamematory condition involving dental implants, surrounding mucosa and bone, which lose supporting bone. Although high success rates for endosseous implants have been reported, failures occur, and some implants are lost or removed. At least 10% of the failures have been suggested to be the result of peri-implantitis. One of the major causes of the peri-implantitis is the bacterial colonization of implant surfaces but additional risk factors such as periodontitis, poor oral hygiene, tobacco consumption, prepost operative therapies and genetic susceptibility should be considered. In the present study a real-time PCR bases assay was designed to detect and quantify red complex species, then used to investigate 307 periodontal pocket samples from 127 periodontitis patients and 180 controls. Results demonstrated a significant higher prevalence of red complex species and increased amount of Porphyromonas gingivalis and Treponema denticolain periodontal pocket of periodontitis. Since a higher risk of peri-implantitis occurs in periodontally affected patients, detection and treatment of bacteria is a fundamental objective to ensure dental implant survival.
文摘Bioevolution is still a main puzzle and attracts many scientists to research on it. Here I present that organisms have two impor- tant properties, definite structure and self-reproduction. Based on the number and connection of the structural units, organisms can be di- vided into three groups, unicellular, particellular and polycellular organisms. It can be called polycellular evolution that organisms evolve from unicellular, particellular to polycellular. Also it can be called diploid evolution that organisms evolve from haplobes to diplobes, two groups based on the reproductive differences. Some concepts like spore, zygote and embryos are redefined in the paper. Moreover, I present that embryos are the most important boundary of bioevolution and organisms can be divided into two evolutionary phases, the lower and the higher. The lower organisms, Kingdom Microbia (kingd. nov.), are inembryonate, which include Acytophyla (phyl. nov.), bacteria, protozoa, fungi and inembryonate algae. The higher organisms are embryonate and have two branches, Kingdom Plantae and Kingdom Animalia. Plantae are sessile and, autotrophic or sporogenic, which include higher plants and Nudembryophyta (phyl. nov.). Animalia are heterotrophic and, motile or gametogenic, which include all multicellular animals. The new system, which reflects the two important phases of bioevolu- tion and two branches of higher organisms, can really correct the problem of different kingdoms in different researches or by different re- searchers.
