In late May 2016, a cyanobacterial harmful algal bloom(cHAB) was detected in the Maumee River, the largest tributary to Lake Erie, the southernmost lake of the Laurentian Great Lakes system. Testing on 31 May identifi...In late May 2016, a cyanobacterial harmful algal bloom(cHAB) was detected in the Maumee River, the largest tributary to Lake Erie, the southernmost lake of the Laurentian Great Lakes system. Testing on 31 May identified Planktothrix agardhii as the dominant cyanobacterium with cell abundance exceeding 1.7×10~9 cells/L and total microcystins(MC) reaching 19 μg/L MC-LR equivalents, a level over 10-fold higher than the 2015 revised U.S. Environmental Protection Agency(EPA) national health advisory levels for drinking water exposure to adults. Low river discharge coincident with negligible precipitation through the latter half of May coincided with an 80% decline in river turbidity that likely favored bloom formation by a low-light adapted P. agardhii population. Also contributing to the c HAB were high initial nutrient loads and an increase of the river temperature from 13℃ to 26℃ over this same period. The bloom persisted through 5 June with microcystins exceeding 22 μg/L MC-LR equivalents at the bloom peak. By 6 June, the river had returned to its muddy character following a rain event and sampling on 7 June detected only low levels of toxin(<0.6 μg/L) at public water systems located near the bloom origin. The elevated toxin production associated with this early onset bloom was without precedent for the Maumee River and an unique attribute of the c HAB was the high proportion of potentially-toxic genotypes. Whereas Planktothrix spp. is common in lotic environments, and has been previously detected in the Maumee, blooms are not commonly reported. This early onset, microcystin-producing c HAB provided a rare opportunity to glean insights into environmental factors that promote bloom development and dominance by Planktothrix in lotic environments.展开更多
Previously, we showed that prophylactic addition of glucose to Harsha Lake water samples could inhibit cyanobacteria growth, at least for a short period of time. The current study tested cyanobacterial control with gl...Previously, we showed that prophylactic addition of glucose to Harsha Lake water samples could inhibit cyanobacteria growth, at least for a short period of time. The current study tested cyanobacterial control with glucose for the entire Harsha Lake bloom season. Water samples (1000 ml) were collected weekly from Harsha Lake during the algal-bloom season starting June 9 and lasting until August 24, 2022. To each of two 7-liter polypropylene containers, 500 ml of Harsha Lake water was added, and the containers were placed in a controlled environment chamber. To one container labeled “Treated,” 0.15 g of glucose was added, and nothing was added to the container labeled “Control.” After that, three 25 ml samples from each container were collected and used for 16S rRNA gene sequencing each week. Then 1000 ml of Harsha Lake water was newly collected each week, with 500 ml added to each container, along with the addition of 0.15 g glucose to the “Treated” container. Sequencing data were used to examine differences in the composition of bacterial communities between Treated and Control containers. Treatment with glucose altered the microbial communities by 1) reducing taxonomic diversity, 2) largely eliminating cyanobacterial taxa, and 3) increasing the relative abundance of subsets of non-cyanobacterial taxa (such as Proteobacteria and Actinobacteriota). These effects were observed across time despite weekly inputs derived directly from Lake water. The addition of glucose to a container receiving weekly additions of Lake water suppressed the cyanobacterial populations during the entire summer bloom season. The glucose appears to stimulate the diversity of certain bacterial taxa at the expense of the cyanobacteria.展开更多
Cyanobacteria-based activated carbon(CBAC)was successfully prepared by pyrolysis-activation of Taihu cyanobacteria.When the impregnation ratio and activated temperature were 2 and 800-C,respectively,the optimal CBACs ...Cyanobacteria-based activated carbon(CBAC)was successfully prepared by pyrolysis-activation of Taihu cyanobacteria.When the impregnation ratio and activated temperature were 2 and 800-C,respectively,the optimal CBACs possessed an ultra-high specific surface(2178.90 m^(2)·g^(-1))and plenty of micro-and meso-pores,as well as a high pore volume(1.01 cm^(3)·g^(-1)).Ascribed to ultra-high surface area,π-π interaction,electrostatic interaction,as well as hydrogen-bonding interactions,the CBACs displayed huge superiority in efficient dye removal.The saturated methylene blue adsorption capacity by CBACs could be as high as 1143.4 mg·g^(-1),superior to that of other reported biomass-activated carbons.The adsorption was endothermic and modeled well by the pseudo-second-order kinetic,intra-particle diffusion,and Langmuir models.This work presented the effectiveness of Taihu cyanobacteria adsorbent ascribed to its super large specific surface area and high adsorption ability.展开更多
The combined use of morphological identification and phylogenetic characterization employing primers that target the 16S rDNA region led to the identification of ten isolates belonging to eight cyanobacterial genera i...The combined use of morphological identification and phylogenetic characterization employing primers that target the 16S rDNA region led to the identification of ten isolates belonging to eight cyanobacterial genera in the Nile River. 16S-23S ITS region was amplified to confirm two isolates to be affiliated to genus Nostoc. Using MALDI-TOF/MS, we detected the production of the hepatotoxic demethylated MC-LR by one isolate that clustered together with the genus Nostoc. Protein phosphatase inhibition assay has confirmed toxicity. Our results add to the rising importance of Nostoc as a hepatotoxin-producing cyanobacterium. Furthermore, our results stress that water municipalities in the studied region need to assess the potential threat of toxic cyanobacteria that may pose to human health and economy.展开更多
Antarctic continental lakes and ponds are among the most impoverished aquatic environments on earth but many of them support flourishing populations of cyanobacteria,eukaryotic algae,protozoans,and some multicellular ...Antarctic continental lakes and ponds are among the most impoverished aquatic environments on earth but many of them support flourishing populations of cyanobacteria,eukaryotic algae,protozoans,and some multicellular animals.In this study,we present results of a microscopic analysis of cyanobacteria and eukaryotic algae from nine diverse types of Antarctic continental water bodies during one austral summer.The results supplement and enlarge our previous studies on the limnological characteristics of the epiglacial and supraglacial lakes and ponds in Dronning Maud Land,an area that has received little attention from limnologists.The taxon with highest frequency among the samples(n=79)was Mesotaenium cf.berggrenii,a eukaryotic Zygnematophyceae,which occurred in 82%of the samples with a maximum cell density of 68 cells·mL^(-1).The taxa with second and third highest frequency were the prokaryotes Gloeocapsopsis(60%)and Leptolyngbya(41%),followed by Chlamydomonas(34%)and Cyanothece(29%).The number of taxa varied between 7-21 among the lakes and ponds,being highest in a supraglacial lake,and lowest in an epiglacial lake.The results did not reveal any obvious correlation between the abundance of any taxa and the water chemistry,but water bodies with inorganic sediments had higher cell densities and biomasses than those without sediment.This suggests the importance of sediment in supporting biological diversity in these ultraoligotrophic lakes and ponds.展开更多
The Mediterranean basin is considered one of the most vulnerable areas in the world under the impacts of global warming and changes in precipitation patterns.Oubeira,a shallow and polymictic freshwater lake located in...The Mediterranean basin is considered one of the most vulnerable areas in the world under the impacts of global warming and changes in precipitation patterns.Oubeira,a shallow and polymictic freshwater lake located in northeastern Algeria(36°50′N,08°23′E),has recently experienced a significant proliferation of harmful cyanobacteria resulting in the generation of toxins.We carried out this study in order to understand the succession patterns of dominant cyanobacteria and cyanotoxin production and the factors driving this in Oubeira Lake.A total of 26 cyanobacterial genera were identified,and among them Microcystis and Planktothrix accounted for more than 60%of the overall cell abundance.The summer/fall period was dominated by Microcystis,Planktothrix,and in lesser extend by Cylindrospermum,Cylindrospermopsis.During the fall/winter transition,Dolichospermum,Pseudanabaena,and Aphanizomenon were the dominant genera.Statistically,the bloom-forming cyanobacteria showed significant differences between months but not between stations.Intracellular microcystins(MC-LR)was detected in all collected samples(0.62-and 19.14-μg MC-LR equivalent/L),but appeared in high concentrations throughout the period of dominance of Microcystis and Planktothrix.Microcystis was more sensitive to nutrients than to temperature.Planktothrix was more dependent on temperature than on nutrients,which explains their coexistence during summer-fall period.However,both genera are positively correlated with MC-LR and would probably be the main producers of microcystins.Pseudanabaena,Dolichospermum,and Aphanizomenon co-occurred at the end of Planktothrix dominance period(December).Aphanizomenon and Pseudanabaena were correlated negatively with temperature and positively with water conductivity.Dolichospermum showed a strong positive correlation with MC-LR.Oubeira Lake,could serve as a model of how cyanobacteria blooms may develop in lakes within Mediterranean climates.展开更多
The Mesozoic and Cenozoic strata in the Junggar basin developed two sets of shallow to semi-deep lacustrine shale, namely, the Cretaceous Qingshuihe Formation (K_(1q)) and the Paleogene Anjihaihe Formation (E_(2-3a))....The Mesozoic and Cenozoic strata in the Junggar basin developed two sets of shallow to semi-deep lacustrine shale, namely, the Cretaceous Qingshuihe Formation (K_(1q)) and the Paleogene Anjihaihe Formation (E_(2-3a)). Through organic petrology and scanning electron microscope (SEM) observation, it is found that the primary hydrocarbon-generating organic matter (OM) in the two sets of strata is different. The biological precursor of the E_(2-3a) OM is mainly green algae (Pediastrum), while the precursor of K_(1q) kerogen is mainly cyanobacteria (Oscillatoria). Then, the E_(2-3a) green algae-rich shale and K_(1q) cyanobacteria-rich shale were subjected to hydrous pyrolysis and kinetic analysis, respectively. The results show that the evolution modes of hydrocarbon generation of the typical shales are very different. Green algae have the characteristics of a low oil generation threshold, heavy oil quality, and no prominent oil peak, while cyanobacteria have the characteristics of late oil generation, concentrated hydrocarbon generation, and relatively light oil quality. The characteristics of oil generation can also be well reflected in the composition evolution of the crude oil components. The carbon isotope of gas, kerogen, and extracts of the E_(2-3a) green algae-rich shale are significantly heavier than the K_(1q) cyanobacteria-rich shale, which may be related to the living habits of their biological precursors, carbon source usage, photosynthesis efficiency, and carbon fixation efficiency.展开更多
热带—亚热带潮间带沉积物中丰富的菌—藻类微生物及其复杂的生命活动对颗粒的早期胶结过程起重要作用。本文通过16S r RNA高通量基因测序方法分析西沙群岛石屿、全富岛及海南龙湾珊瑚礁滨岸带海滩岩表生微生物群落结构,确定主导海滩岩...热带—亚热带潮间带沉积物中丰富的菌—藻类微生物及其复杂的生命活动对颗粒的早期胶结过程起重要作用。本文通过16S r RNA高通量基因测序方法分析西沙群岛石屿、全富岛及海南龙湾珊瑚礁滨岸带海滩岩表生微生物群落结构,确定主导海滩岩快速胶结的共性菌株/微生物功能群。表层生长草皮海藻的全富岛海滩岩原核生物多样性最高。龙湾潮间带上部暴露时间较长的海滩岩比潮间带下部海滩岩原核生物多样性高。相对固结的石屿海滩岩原核生物多样性最低。4个取样点具有较为相似的细菌门类组成,物种丰度由大到小依次为变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteriota)、蓝细菌门(Cyanobacteria)、绿弯菌门(Chloroflexi)、芽单胞菌门(Gemmatimonadota)和脱硫杆菌门(Desulfobacterota)。不同点位的海滩岩形成环境中蓝细菌主要种类大体相同,其中眉藻(Calothrixsp.)是主导海滩岩早期胶结的共性钙化菌株,通过分泌胞外聚合物成为碳酸盐矿物沉淀的“热点”。群落中检出的反硝化细菌和硫酸盐还原菌表明海滩岩内部氧化还原梯度较大,表面下极浅处即存在缺氧微环境。微生物群落内强烈的光合作用、硫酸盐还原作用和反硝化作用不断提高微环境pH值,有利碳酸盐矿物在粒间孔内的成核和生长并加速颗粒胶结。展开更多
基金Supported by a Harmful Algal Bloom Research Initiative Grant(No.R/HAB-2-ODHE)from the Ohio Department of Higher Education(GSB,RMLM)Ohio Sea Grant College Program grant R/ER-114(GSB,RMLM)
文摘In late May 2016, a cyanobacterial harmful algal bloom(cHAB) was detected in the Maumee River, the largest tributary to Lake Erie, the southernmost lake of the Laurentian Great Lakes system. Testing on 31 May identified Planktothrix agardhii as the dominant cyanobacterium with cell abundance exceeding 1.7×10~9 cells/L and total microcystins(MC) reaching 19 μg/L MC-LR equivalents, a level over 10-fold higher than the 2015 revised U.S. Environmental Protection Agency(EPA) national health advisory levels for drinking water exposure to adults. Low river discharge coincident with negligible precipitation through the latter half of May coincided with an 80% decline in river turbidity that likely favored bloom formation by a low-light adapted P. agardhii population. Also contributing to the c HAB were high initial nutrient loads and an increase of the river temperature from 13℃ to 26℃ over this same period. The bloom persisted through 5 June with microcystins exceeding 22 μg/L MC-LR equivalents at the bloom peak. By 6 June, the river had returned to its muddy character following a rain event and sampling on 7 June detected only low levels of toxin(<0.6 μg/L) at public water systems located near the bloom origin. The elevated toxin production associated with this early onset bloom was without precedent for the Maumee River and an unique attribute of the c HAB was the high proportion of potentially-toxic genotypes. Whereas Planktothrix spp. is common in lotic environments, and has been previously detected in the Maumee, blooms are not commonly reported. This early onset, microcystin-producing c HAB provided a rare opportunity to glean insights into environmental factors that promote bloom development and dominance by Planktothrix in lotic environments.
文摘Previously, we showed that prophylactic addition of glucose to Harsha Lake water samples could inhibit cyanobacteria growth, at least for a short period of time. The current study tested cyanobacterial control with glucose for the entire Harsha Lake bloom season. Water samples (1000 ml) were collected weekly from Harsha Lake during the algal-bloom season starting June 9 and lasting until August 24, 2022. To each of two 7-liter polypropylene containers, 500 ml of Harsha Lake water was added, and the containers were placed in a controlled environment chamber. To one container labeled “Treated,” 0.15 g of glucose was added, and nothing was added to the container labeled “Control.” After that, three 25 ml samples from each container were collected and used for 16S rRNA gene sequencing each week. Then 1000 ml of Harsha Lake water was newly collected each week, with 500 ml added to each container, along with the addition of 0.15 g glucose to the “Treated” container. Sequencing data were used to examine differences in the composition of bacterial communities between Treated and Control containers. Treatment with glucose altered the microbial communities by 1) reducing taxonomic diversity, 2) largely eliminating cyanobacterial taxa, and 3) increasing the relative abundance of subsets of non-cyanobacterial taxa (such as Proteobacteria and Actinobacteriota). These effects were observed across time despite weekly inputs derived directly from Lake water. The addition of glucose to a container receiving weekly additions of Lake water suppressed the cyanobacterial populations during the entire summer bloom season. The glucose appears to stimulate the diversity of certain bacterial taxa at the expense of the cyanobacteria.
基金financially supported by the Key Research&Development program of Zhejiang Province(2021C03196)the National Key Research and Development Program of China(2022YFE0128600)the Natural Science Foundation of Zhejiang Province(LY22B060011).
文摘Cyanobacteria-based activated carbon(CBAC)was successfully prepared by pyrolysis-activation of Taihu cyanobacteria.When the impregnation ratio and activated temperature were 2 and 800-C,respectively,the optimal CBACs possessed an ultra-high specific surface(2178.90 m^(2)·g^(-1))and plenty of micro-and meso-pores,as well as a high pore volume(1.01 cm^(3)·g^(-1)).Ascribed to ultra-high surface area,π-π interaction,electrostatic interaction,as well as hydrogen-bonding interactions,the CBACs displayed huge superiority in efficient dye removal.The saturated methylene blue adsorption capacity by CBACs could be as high as 1143.4 mg·g^(-1),superior to that of other reported biomass-activated carbons.The adsorption was endothermic and modeled well by the pseudo-second-order kinetic,intra-particle diffusion,and Langmuir models.This work presented the effectiveness of Taihu cyanobacteria adsorbent ascribed to its super large specific surface area and high adsorption ability.
文摘The combined use of morphological identification and phylogenetic characterization employing primers that target the 16S rDNA region led to the identification of ten isolates belonging to eight cyanobacterial genera in the Nile River. 16S-23S ITS region was amplified to confirm two isolates to be affiliated to genus Nostoc. Using MALDI-TOF/MS, we detected the production of the hepatotoxic demethylated MC-LR by one isolate that clustered together with the genus Nostoc. Protein phosphatase inhibition assay has confirmed toxicity. Our results add to the rising importance of Nostoc as a hepatotoxin-producing cyanobacterium. Furthermore, our results stress that water municipalities in the studied region need to assess the potential threat of toxic cyanobacteria that may pose to human health and economy.
基金supported by AF-NSFC mobility program from the Academy of Finland(Grant no.333170)National Natural Science Foundation of China(Grant no.52211530038).
文摘Antarctic continental lakes and ponds are among the most impoverished aquatic environments on earth but many of them support flourishing populations of cyanobacteria,eukaryotic algae,protozoans,and some multicellular animals.In this study,we present results of a microscopic analysis of cyanobacteria and eukaryotic algae from nine diverse types of Antarctic continental water bodies during one austral summer.The results supplement and enlarge our previous studies on the limnological characteristics of the epiglacial and supraglacial lakes and ponds in Dronning Maud Land,an area that has received little attention from limnologists.The taxon with highest frequency among the samples(n=79)was Mesotaenium cf.berggrenii,a eukaryotic Zygnematophyceae,which occurred in 82%of the samples with a maximum cell density of 68 cells·mL^(-1).The taxa with second and third highest frequency were the prokaryotes Gloeocapsopsis(60%)and Leptolyngbya(41%),followed by Chlamydomonas(34%)and Cyanothece(29%).The number of taxa varied between 7-21 among the lakes and ponds,being highest in a supraglacial lake,and lowest in an epiglacial lake.The results did not reveal any obvious correlation between the abundance of any taxa and the water chemistry,but water bodies with inorganic sediments had higher cell densities and biomasses than those without sediment.This suggests the importance of sediment in supporting biological diversity in these ultraoligotrophic lakes and ponds.
基金Supported by the General Directorate for Scientific Research and Technological DevelopmentAlgeria (No.D00L03UN230120150002)。
文摘The Mediterranean basin is considered one of the most vulnerable areas in the world under the impacts of global warming and changes in precipitation patterns.Oubeira,a shallow and polymictic freshwater lake located in northeastern Algeria(36°50′N,08°23′E),has recently experienced a significant proliferation of harmful cyanobacteria resulting in the generation of toxins.We carried out this study in order to understand the succession patterns of dominant cyanobacteria and cyanotoxin production and the factors driving this in Oubeira Lake.A total of 26 cyanobacterial genera were identified,and among them Microcystis and Planktothrix accounted for more than 60%of the overall cell abundance.The summer/fall period was dominated by Microcystis,Planktothrix,and in lesser extend by Cylindrospermum,Cylindrospermopsis.During the fall/winter transition,Dolichospermum,Pseudanabaena,and Aphanizomenon were the dominant genera.Statistically,the bloom-forming cyanobacteria showed significant differences between months but not between stations.Intracellular microcystins(MC-LR)was detected in all collected samples(0.62-and 19.14-μg MC-LR equivalent/L),but appeared in high concentrations throughout the period of dominance of Microcystis and Planktothrix.Microcystis was more sensitive to nutrients than to temperature.Planktothrix was more dependent on temperature than on nutrients,which explains their coexistence during summer-fall period.However,both genera are positively correlated with MC-LR and would probably be the main producers of microcystins.Pseudanabaena,Dolichospermum,and Aphanizomenon co-occurred at the end of Planktothrix dominance period(December).Aphanizomenon and Pseudanabaena were correlated negatively with temperature and positively with water conductivity.Dolichospermum showed a strong positive correlation with MC-LR.Oubeira Lake,could serve as a model of how cyanobacteria blooms may develop in lakes within Mediterranean climates.
基金supported by Xinjiang Oilfield Company of China(No.2020-C4006).
文摘The Mesozoic and Cenozoic strata in the Junggar basin developed two sets of shallow to semi-deep lacustrine shale, namely, the Cretaceous Qingshuihe Formation (K_(1q)) and the Paleogene Anjihaihe Formation (E_(2-3a)). Through organic petrology and scanning electron microscope (SEM) observation, it is found that the primary hydrocarbon-generating organic matter (OM) in the two sets of strata is different. The biological precursor of the E_(2-3a) OM is mainly green algae (Pediastrum), while the precursor of K_(1q) kerogen is mainly cyanobacteria (Oscillatoria). Then, the E_(2-3a) green algae-rich shale and K_(1q) cyanobacteria-rich shale were subjected to hydrous pyrolysis and kinetic analysis, respectively. The results show that the evolution modes of hydrocarbon generation of the typical shales are very different. Green algae have the characteristics of a low oil generation threshold, heavy oil quality, and no prominent oil peak, while cyanobacteria have the characteristics of late oil generation, concentrated hydrocarbon generation, and relatively light oil quality. The characteristics of oil generation can also be well reflected in the composition evolution of the crude oil components. The carbon isotope of gas, kerogen, and extracts of the E_(2-3a) green algae-rich shale are significantly heavier than the K_(1q) cyanobacteria-rich shale, which may be related to the living habits of their biological precursors, carbon source usage, photosynthesis efficiency, and carbon fixation efficiency.
文摘热带—亚热带潮间带沉积物中丰富的菌—藻类微生物及其复杂的生命活动对颗粒的早期胶结过程起重要作用。本文通过16S r RNA高通量基因测序方法分析西沙群岛石屿、全富岛及海南龙湾珊瑚礁滨岸带海滩岩表生微生物群落结构,确定主导海滩岩快速胶结的共性菌株/微生物功能群。表层生长草皮海藻的全富岛海滩岩原核生物多样性最高。龙湾潮间带上部暴露时间较长的海滩岩比潮间带下部海滩岩原核生物多样性高。相对固结的石屿海滩岩原核生物多样性最低。4个取样点具有较为相似的细菌门类组成,物种丰度由大到小依次为变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteriota)、蓝细菌门(Cyanobacteria)、绿弯菌门(Chloroflexi)、芽单胞菌门(Gemmatimonadota)和脱硫杆菌门(Desulfobacterota)。不同点位的海滩岩形成环境中蓝细菌主要种类大体相同,其中眉藻(Calothrixsp.)是主导海滩岩早期胶结的共性钙化菌株,通过分泌胞外聚合物成为碳酸盐矿物沉淀的“热点”。群落中检出的反硝化细菌和硫酸盐还原菌表明海滩岩内部氧化还原梯度较大,表面下极浅处即存在缺氧微环境。微生物群落内强烈的光合作用、硫酸盐还原作用和反硝化作用不断提高微环境pH值,有利碳酸盐矿物在粒间孔内的成核和生长并加速颗粒胶结。
