Angiogenesis is a key process in regenerative medicine generally, as well as in the specific field of nerve regeneration. However, no convenient and objective method for evaluating the angiogenesis of tissue-engineere...Angiogenesis is a key process in regenerative medicine generally, as well as in the specific field of nerve regeneration. However, no convenient and objective method for evaluating the angiogenesis of tissue-engineered nerves has been reported. In this study, tissue-engineered nerves were constructed in vitro using Schwann cells differentiated from rat skin-derived precursors as supporting cells and chitosan nerve conduits combined with silk fibroin fibers as scaffolds to bridge 10-mm sciatic nerve defects in rats. Four weeks after surgery, three-dimensional blood vessel reconstructions were made through MICROFIL perfusion and micro-CT scanning, and parameter analysis of the tissue-engineered nerves was performed. New blood vessels grew into the tissue-engineered nerves from three main directions： the proximal end, the distal end, and the middle. The parameter analysis of the three-dimensional blood vessel images yielded several parameters, including the number, diameter, connection, and spatial distribution of blood vessels. The new blood vessels were mainly capillaries and microvessels, with diameters ranging from 9 to 301 μm. The blood vessels with diameters from 27 to 155 μm accounted for 82.84% of the new vessels. The microvessels in the tissue-engineered nerves implanted in vivo were relatively well-identified using the MICROFIL perfusion and micro-CT scanning method, which allows the evaluation and comparison of differences and changes of angiogenesis in tissue-engineered nerves implanted in vivo.展开更多
Thin microfll - polypropylene knitted fabric that is knitted to use new single plated theory on large diameter circular weft knitting machine is a new functional fabric. After military use, the reflective opinions are...Thin microfll - polypropylene knitted fabric that is knitted to use new single plated theory on large diameter circular weft knitting machine is a new functional fabric. After military use, the reflective opinions are that it is light, thin, soft, smooth - touch, and it has excellent elasticity, good air perviousness, large water vapor permeability . In this paper, an analysis is held to use grey system theory, and the thermohydro - comfort durability of this microfil - polypropylene knitted fabric is compared with that of common knitted fabrics. The analytical result proves the microfil - polypropylene knitted fabric is satisfactory under the climatic conditions that human body needs to eliminate or retain the quantity of heat. It can be use to seam up not only physical ability training uniform of serviceman but also civil sporty and sportswear et cetera.展开更多
基金supported by the National Natural Science Foundation of ChinaNo.81130080
文摘Angiogenesis is a key process in regenerative medicine generally, as well as in the specific field of nerve regeneration. However, no convenient and objective method for evaluating the angiogenesis of tissue-engineered nerves has been reported. In this study, tissue-engineered nerves were constructed in vitro using Schwann cells differentiated from rat skin-derived precursors as supporting cells and chitosan nerve conduits combined with silk fibroin fibers as scaffolds to bridge 10-mm sciatic nerve defects in rats. Four weeks after surgery, three-dimensional blood vessel reconstructions were made through MICROFIL perfusion and micro-CT scanning, and parameter analysis of the tissue-engineered nerves was performed. New blood vessels grew into the tissue-engineered nerves from three main directions： the proximal end, the distal end, and the middle. The parameter analysis of the three-dimensional blood vessel images yielded several parameters, including the number, diameter, connection, and spatial distribution of blood vessels. The new blood vessels were mainly capillaries and microvessels, with diameters ranging from 9 to 301 μm. The blood vessels with diameters from 27 to 155 μm accounted for 82.84% of the new vessels. The microvessels in the tissue-engineered nerves implanted in vivo were relatively well-identified using the MICROFIL perfusion and micro-CT scanning method, which allows the evaluation and comparison of differences and changes of angiogenesis in tissue-engineered nerves implanted in vivo.
基金Hubei Province Key Program, The Development of Microfil - polypropylene Knitted Fabric
文摘Thin microfll - polypropylene knitted fabric that is knitted to use new single plated theory on large diameter circular weft knitting machine is a new functional fabric. After military use, the reflective opinions are that it is light, thin, soft, smooth - touch, and it has excellent elasticity, good air perviousness, large water vapor permeability . In this paper, an analysis is held to use grey system theory, and the thermohydro - comfort durability of this microfil - polypropylene knitted fabric is compared with that of common knitted fabrics. The analytical result proves the microfil - polypropylene knitted fabric is satisfactory under the climatic conditions that human body needs to eliminate or retain the quantity of heat. It can be use to seam up not only physical ability training uniform of serviceman but also civil sporty and sportswear et cetera.
