Cell metabolite analysis is of great interest to analytical chemists and physiologists, with some metabolites having been identified as important indicators of major diseases such as cancer. A highthroughput and sensi...Cell metabolite analysis is of great interest to analytical chemists and physiologists, with some metabolites having been identified as important indicators of major diseases such as cancer. A highthroughput and sensitive method for drug metabolite analysis will largely promote the drug discovery industry. The basic barrier of metabolite analysis comes from the interference of complex components in cell biological system and low abundance of target substances. As a powerful tool in biosample analysis, microfluidic chip enhances the sensitivity and throughput by integrating multiple functional units into one chip. In this review, we discussed three critical steps of establishing functional microfluidic platform for cellular metabolism study. Cell in vitro culture model, on chip sample pretreatment, and microchip combined detectors were described in details and demonstrated by works in five years. And a brief summary was given to discuss the advantages as well as challenges of applying microchip method in cell metabolite and biosample analysis.展开更多
In this study,we developed a microfluidic paper analysis device(μPAD)for distance-based detection of Ag^(+)in water.TheμPAD was manufactured by wax printing method on filter paper.Then,a layer of gold nanoparticles(...In this study,we developed a microfluidic paper analysis device(μPAD)for distance-based detection of Ag^(+)in water.TheμPAD was manufactured by wax printing method on filter paper.Then,a layer of gold nanoparticles(AuNPs)was deposited and ascorbic acid was printed on the channel.In the detection,Ag^(+)was reduced by ascorbic acid and coated on the surface of the AuNPs on the channel,forming Au@Ag core/shell nanoparticles.Based on the capillary flow principle,diff erent concentrations of Ag^(+)formed diff erent distances of color ribbons.Thus,quantitative detection of Ag^(+)can be achieved by measuring the distance of the color ribbon.The detection limit of this method was as low as 1 mg·L^(-1)within 15 min and the interference of common metal ions in water can be eliminated.In conclusion,this method had successfully realized the leap from colorimetry to direct reading,realizing fast read and easy manipulation with low-cost.展开更多
基金financially supported by National Natural Science Foundation of China(Nos.8137337391213305+1 种基金21227006)CERS–China Equipment and Education Resources System(No.CERS-1-75)
文摘Cell metabolite analysis is of great interest to analytical chemists and physiologists, with some metabolites having been identified as important indicators of major diseases such as cancer. A highthroughput and sensitive method for drug metabolite analysis will largely promote the drug discovery industry. The basic barrier of metabolite analysis comes from the interference of complex components in cell biological system and low abundance of target substances. As a powerful tool in biosample analysis, microfluidic chip enhances the sensitivity and throughput by integrating multiple functional units into one chip. In this review, we discussed three critical steps of establishing functional microfluidic platform for cellular metabolism study. Cell in vitro culture model, on chip sample pretreatment, and microchip combined detectors were described in details and demonstrated by works in five years. And a brief summary was given to discuss the advantages as well as challenges of applying microchip method in cell metabolite and biosample analysis.
基金supported by the Graduate Student Innovation Project of China University of Petroleum(East China)in 2020(No.YCX2020054)the financial support by the National Natural Science Foundation of China(No.21876206,21505157)+1 种基金the Key Fundamental Research Fund of Shandong Province(ZR2020ZD13)the Youth Innovation and Technology projects of Universities in Shandong Province(2020KJC007,ZR2020MB064)
文摘In this study,we developed a microfluidic paper analysis device(μPAD)for distance-based detection of Ag^(+)in water.TheμPAD was manufactured by wax printing method on filter paper.Then,a layer of gold nanoparticles(AuNPs)was deposited and ascorbic acid was printed on the channel.In the detection,Ag^(+)was reduced by ascorbic acid and coated on the surface of the AuNPs on the channel,forming Au@Ag core/shell nanoparticles.Based on the capillary flow principle,diff erent concentrations of Ag^(+)formed diff erent distances of color ribbons.Thus,quantitative detection of Ag^(+)can be achieved by measuring the distance of the color ribbon.The detection limit of this method was as low as 1 mg·L^(-1)within 15 min and the interference of common metal ions in water can be eliminated.In conclusion,this method had successfully realized the leap from colorimetry to direct reading,realizing fast read and easy manipulation with low-cost.
