In this work,two kinds of novel microfluidic cells separation chips were described on the principle of crossflow filtration by using micro-electro mechanical systems(MEMS) technology.Whole blood is a complex mixture o...In this work,two kinds of novel microfluidic cells separation chips were described on the principle of crossflow filtration by using micro-electro mechanical systems(MEMS) technology.Whole blood is a complex mixture of various cells,such as red blood cells(RBC),white blood cells(WBC) and so on.Separation and collection of different kinds of cells is the required first step for the subsequent clinical and basic research assays.One-level filtration microfluidic chips with three coiled channels spaced by two arrays of parallel microfabricated filtration barriers of post-type or dam-type along the stream,were successfully used to separate and collect RBC and WBC from whole blood via their different sizes.The effects of the dilution times and the length of separation channels with different filtration barriers on RBC separation efficiency were investigated.When the whole blood was diluted by 50 times,the RBC separation efficiency was 91.2% and the WBC separation efficiency was 27.4% by using the microfluidic chip with dam-type filtration barriers of 160 mm long.The WBC separation efficiency was two times more than that by using microfluidic chip based on the dead-end filtration principle.The second kind of microfluidic chip had multilevel filtration barriers of post-type which was used to separate and collect plasma,RBC and WBC from whole blood.展开更多
A novel microfluidic chip is developed for crossflow filtration plasma from the whole blood which is carried out in a continuous manner. This microfluidic chip was made of a silicon substrate sealed with a compound co...A novel microfluidic chip is developed for crossflow filtration plasma from the whole blood which is carried out in a continuous manner. This microfluidic chip was made of a silicon substrate sealed with a compound cover. The silicon substrate fabricated by micro-electro-mechanical system (MEMS) technology consisted of microposts array, microchannels and reservoirs. Then the silicon substrate was characterized by Scaning Electron Microscopy (SEM). The performance of the microfluidic chip was valued by the experiments of plasma isolation. During more than one hour of continuous blood infusion through the chip, there were no problems of jamming or clogging, and the plasma selectivity of 97.78% was achieved. Due to the chip’s simple structure and control mechanism with a continuous, real time operating manner, this microfluidic chip is easily expected to be integrated into micro total analytical system (μTAS) which will create a microanalysis system for point-of-care diagnostics.展开更多
文摘In this work,two kinds of novel microfluidic cells separation chips were described on the principle of crossflow filtration by using micro-electro mechanical systems(MEMS) technology.Whole blood is a complex mixture of various cells,such as red blood cells(RBC),white blood cells(WBC) and so on.Separation and collection of different kinds of cells is the required first step for the subsequent clinical and basic research assays.One-level filtration microfluidic chips with three coiled channels spaced by two arrays of parallel microfabricated filtration barriers of post-type or dam-type along the stream,were successfully used to separate and collect RBC and WBC from whole blood via their different sizes.The effects of the dilution times and the length of separation channels with different filtration barriers on RBC separation efficiency were investigated.When the whole blood was diluted by 50 times,the RBC separation efficiency was 91.2% and the WBC separation efficiency was 27.4% by using the microfluidic chip with dam-type filtration barriers of 160 mm long.The WBC separation efficiency was two times more than that by using microfluidic chip based on the dead-end filtration principle.The second kind of microfluidic chip had multilevel filtration barriers of post-type which was used to separate and collect plasma,RBC and WBC from whole blood.
基金Supported by National Natural Science Foundation of China (Grant No. 60701019 and 605010200)National High Technology Research and Development Program of China (Grant No. 2006AAQ4Z355)the Knowledge Innovation Program of Chinese Academy of Sciences
文摘A novel microfluidic chip is developed for crossflow filtration plasma from the whole blood which is carried out in a continuous manner. This microfluidic chip was made of a silicon substrate sealed with a compound cover. The silicon substrate fabricated by micro-electro-mechanical system (MEMS) technology consisted of microposts array, microchannels and reservoirs. Then the silicon substrate was characterized by Scaning Electron Microscopy (SEM). The performance of the microfluidic chip was valued by the experiments of plasma isolation. During more than one hour of continuous blood infusion through the chip, there were no problems of jamming or clogging, and the plasma selectivity of 97.78% was achieved. Due to the chip’s simple structure and control mechanism with a continuous, real time operating manner, this microfluidic chip is easily expected to be integrated into micro total analytical system (μTAS) which will create a microanalysis system for point-of-care diagnostics.
