Objective To approach the effect of microgene pSVPoMcat modified Schwan n cell (SC) on the regeneration and repair of injured spinal cord.Method Spinal cord hemi－transection models were made with the cutting method i...Objective To approach the effect of microgene pSVPoMcat modified Schwan n cell (SC) on the regeneration and repair of injured spinal cord.Method Spinal cord hemi－transection models were made with the cutting method in healthy SD ra ts. Microgene pSVPoMcat modified SC(group A),highly purified SC(group B),and glu tin sponge (control group C)were randomly implanted into the cut. After 3 month living ,the host rats were scanned by MRI, and observed under EM. Result Spinal signals at the injury region nearly recovered to normal in group A.No recovery w as found in group B.Malacosis was found in group C.TEM findings: regeneration of large number of myelinated and nonmyelinated axons and SC proliferation in grou p A, myelinated axon regeneration and SC necrosis in group B, non myelinated and nonmyelinated axon in group C.Conclusion Implantation of microgene pSVPoMcat mo dified SC could promote the repair of injured spinal cord.展开更多
Objective: To observe the repair effect of Schwann cells (SCs) modified by microgene pSVPoMcat on injured spinal cord in rats. Methods: Semi transection injury at the level of T 8 of spinal cord was made with cutting ...Objective: To observe the repair effect of Schwann cells (SCs) modified by microgene pSVPoMcat on injured spinal cord in rats. Methods: Semi transection injury at the level of T 8 of spinal cord was made with cutting method on 120 Sprague Dawley (SD) rats. Then 40 rats implanted with SCs modified by microgene pSVPoMcat were taken as Group A, 40 rats implanted with simple SCs as Group B and the other 40 rats were taken as the control group (Group C). The functional recovery of the rats was observed through combined behavioral score (CBS) and cortical somatosensory evoked potential (CSEP), and the expression of the glial fibrillary acidic protein (GFAP) was measured with in situ hybridization and immunocytochemistry. At 3 months after operation, the rats were examined with magnetic resonance image (MRI), and the neurofilaments (NF) of the axons were stained with immunohistochemical method. Results: GFAP expression in Group A was significantly lower than that of the other 2 groups. MRI showed that the spinal signals in the injured area recovered fundamentally in Group A, didnt recover in Group B and malacia focus was found in Group C, which was same as the results of NF staining. Wave amplitudes in incubation periods in Group A and Group B tended to recover. It recovered to the normal level in Group A, which was similar to the results of CBS.Conclusions: SCs modified by microgene pSVPoMcat can inhibit GFAP expression, improve the growth of the axons and the functional recovery of neurons after spinal cord injury.展开更多
文摘Objective To approach the effect of microgene pSVPoMcat modified Schwan n cell (SC) on the regeneration and repair of injured spinal cord.Method Spinal cord hemi－transection models were made with the cutting method in healthy SD ra ts. Microgene pSVPoMcat modified SC(group A),highly purified SC(group B),and glu tin sponge (control group C)were randomly implanted into the cut. After 3 month living ,the host rats were scanned by MRI, and observed under EM. Result Spinal signals at the injury region nearly recovered to normal in group A.No recovery w as found in group B.Malacosis was found in group C.TEM findings: regeneration of large number of myelinated and nonmyelinated axons and SC proliferation in grou p A, myelinated axon regeneration and SC necrosis in group B, non myelinated and nonmyelinated axon in group C.Conclusion Implantation of microgene pSVPoMcat mo dified SC could promote the repair of injured spinal cord.
文摘Objective: To observe the repair effect of Schwann cells (SCs) modified by microgene pSVPoMcat on injured spinal cord in rats. Methods: Semi transection injury at the level of T 8 of spinal cord was made with cutting method on 120 Sprague Dawley (SD) rats. Then 40 rats implanted with SCs modified by microgene pSVPoMcat were taken as Group A, 40 rats implanted with simple SCs as Group B and the other 40 rats were taken as the control group (Group C). The functional recovery of the rats was observed through combined behavioral score (CBS) and cortical somatosensory evoked potential (CSEP), and the expression of the glial fibrillary acidic protein (GFAP) was measured with in situ hybridization and immunocytochemistry. At 3 months after operation, the rats were examined with magnetic resonance image (MRI), and the neurofilaments (NF) of the axons were stained with immunohistochemical method. Results: GFAP expression in Group A was significantly lower than that of the other 2 groups. MRI showed that the spinal signals in the injured area recovered fundamentally in Group A, didnt recover in Group B and malacia focus was found in Group C, which was same as the results of NF staining. Wave amplitudes in incubation periods in Group A and Group B tended to recover. It recovered to the normal level in Group A, which was similar to the results of CBS.Conclusions: SCs modified by microgene pSVPoMcat can inhibit GFAP expression, improve the growth of the axons and the functional recovery of neurons after spinal cord injury.
