Adjuvant chemotherapy improves the survival outlook for patients undergoing operations for lung metastases caused by colorectal cancer (CRC). However, a multidisciplinary approach that evaluates several factors relate...Adjuvant chemotherapy improves the survival outlook for patients undergoing operations for lung metastases caused by colorectal cancer (CRC). However, a multidisciplinary approach that evaluates several factors related to patient and tumor characteristics is necessary for managing chemotherapy treatment in metastatic CRC patients with lung disease, as such factors dictate the timing and drug regimen, which may affect treatment response and prognosis. In this study, we explore the potential of spatial metabolomics for evaluating metabolic phenotypes and therapy outcomes during the local delivery of the anticancer drug, oxaliplatin, to the lung. 12 male Yorkshire pigs underwent a 3 h left lung in vivo lung perfusion (IVLP) with various doses of oxaliplatin (7.5, 10, 20, 40, and 80 mg/L), which were administered to the perfusion circuit reservoir as a bolus. Biocompatible solid-phase microextraction (SPME) microprobes were combined with global metabolite profiling to obtain spatiotemporal information about the activity of the drug, determine toxic doses that exceed therapeutic efficacy, and conduct a mechanistic exploration of associated lung injury. Mild and subclinical lung injury was observed at 40 mg/L of oxaliplatin, and significant compromise of the hemodynamic lung function was found at 80 mg/L. This result was associated with massive alterations in metabolic patterns of lung tissue and perfusate, resulting in a total of 139 discriminant compounds. Uncontrolled inflammatory response, abnormalities in energy metabolism, and mitochondrial dysfunction next to accelerated kynurenine and aldosterone production were recognized as distinct features of dysregulated metabolipidome. Spatial pharmacometabolomics may be a promising tool for identifying pathological responses to chemotherapy.展开更多
A suite of continental flood basalts sampled over a vast exposure and stratigraphic thickness in the Emeishan large igneous province （LIP）, SW China was investigated for laser microprobe ^40Ar/^39Ar dating. There ar...A suite of continental flood basalts sampled over a vast exposure and stratigraphic thickness in the Emeishan large igneous province （LIP）, SW China was investigated for laser microprobe ^40Ar/^39Ar dating. There are two ^40Ar/^39Ar age groups for these basalts, corresponding to 259-246 Ma and 177-137 Ma, respectively. A well-defined isochron gives an eruption age of huge quantities of mafic magmas at 258.9±3.4 Ma, which is identical to previous dating and paleontological data. Much younger ^40Ar/^39Ar ages for some basalts with Iow-greenschist metamorphic facies probably recorded a late thermo-tectonic event caused by collision between the Yangtze and Qiangtang continental blocks during the Mesozoic, which resulted in the reset of argon isotope system. The ^40Ar/^39Ar age data, we present here, combined with previous dating and paleontological data, suggest relatively short duration （about 3 Ma） of mafic volcanism, which have important implication on mantle plume genesis of the Emeishan continental flood basalts in the LIP.展开更多
The Longquanguan shear zone is an important structural belt in the North China Craton, separating the underlying Fuping complex from the overlying Wutai complex. This shear zone has experienced three episodes of defor...The Longquanguan shear zone is an important structural belt in the North China Craton, separating the underlying Fuping complex from the overlying Wutai complex. This shear zone has experienced three episodes of deformation: the first and main episode is a ductile top-to-ESE shear along the gently northwest-west dipping foliations, while the other two episodes are later collapse sliding. Prolonged granites parallel to the shear foliations make one of the main compositions of the Longquanguan shear zone. These granites experienced deformation to form mylonitic rocks when they emplaced during the first episode of deformation. Structural characters of the granites and their contacts to the country rocks indicate that these granites possibly resulted from in-situ partial remelting by shearing, i.e., they are syn-deformational granites. Monazites in these mylonitic granites are magmatic minerals and their crystallization ages may represent ages of the magmatic events, and also the ages for the main deformation of the Longquanguan shear zone. Monazite electronic microprobe dating were carried on two samples of granite, which gives multiple peak ages, among which 1,846 Ma and 1,877 Ma are the main peak ages for the two samples. These ages represent the main deformation of the Longquanguan shear zone, which is consistent with the main regional geological event at about 1,850 Ma caused by the collision between the Eastern and Western Blocks in North China. The good match between the monazite ages and the corresponding regional tectono-thermal events shows the feasibility and reliability of monazite electronic microprobe dating.展开更多
A combination of NAA and micro-PIXE was used to study concentrations and distributions of platinum group elements (PGE) in ores from Xinjie Cu-Pt deposit.The NAA results of the bulk indicate that the ores belong to th...A combination of NAA and micro-PIXE was used to study concentrations and distributions of platinum group elements (PGE) in ores from Xinjie Cu-Pt deposit.The NAA results of the bulk indicate that the ores belong to the enriched Pt-Pd type.The element concentration maps of scanning micro-PIXE for the ores show that the occurence form of Pt is independent arsenide minerals. No PGE were detected in chalcopyrite of Xinjie Cu-Pt deposit. These information are economically beneficial to the mineral smelting process.展开更多
This paper describes the scanning nuclear microprobe facilities established at the Shanghai Institute of Nuclear Research. The Russion quadruplet constructed with four magnetic quadrupolets is used for microbeam forma...This paper describes the scanning nuclear microprobe facilities established at the Shanghai Institute of Nuclear Research. The Russion quadruplet constructed with four magnetic quadrupolets is used for microbeam formation. The long focus of the lens makes the working distance long enough to accommodate the scanning coils and the detectors for PIXE, RBS, RFS, NRA and SE experiments. A 5 μm focussed beam is scanned continously by a pair of coils. A multiparameter multidetector data system for the nuclear microprobe is based on the idea of Total quantitative scanning analysis (TQSA) suggested by Melbourne University. A digital graphic displayer is a good substitute for an ordinary storage oscilloscope for on- line scanned area monitoring. The new microprobe meets high demands of biologists and geologists on applications.展开更多
In order to develop new reference materials for microanalytical nuclear techniques, scanning proton microprobe (SPM) technique was used to determine homogeneity level within 100×200 μm 2 micro area on the small ...In order to develop new reference materials for microanalytical nuclear techniques, scanning proton microprobe (SPM) technique was used to determine homogeneity level within 100×200 μm 2 micro area on the small pieces of IAEA urban dust reference materials. The experimental methods were described in detail. The results show that IAEA 396A/M Vienna urban dust is homogeneous enough for small sample analysis of standard reference material (SRM).展开更多
The morphology of polyvinyl chloride/polystyrene (PVC/PS) blend samples with different mass ratios, prepared by means of solution casting and melt mixing, have been successfully examined by electron microprobe analysi...The morphology of polyvinyl chloride/polystyrene (PVC/PS) blend samples with different mass ratios, prepared by means of solution casting and melt mixing, have been successfully examined by electron microprobe analysis (EMP). This experiment was performed in a scanning electron microscope attached to an energy dispersive X-ray analyzer. Differential scanning calorimetry was also used to investigate the phase separation of the blends. The results show that PVC and PS are incompatible and the blends have sea-islands phase structures. Blends prepared via melt mixing have finer phase-dispersion than those prepared via solution casting.展开更多
An X-ray microprobe for radiobiological studies was developed which deliver precise doses of radiation to the selected individual cells.The facility used synchrotron radiation as soft X-ray source.A zone plate combini...An X-ray microprobe for radiobiological studies was developed which deliver precise doses of radiation to the selected individual cells.The facility used synchrotron radiation as soft X-ray source.A zone plate combining with a pinhole produced a fine probe from bending magnet for single cell irradiating with defined doses.The diameter of microprobe at the target position was about 2 μm by scanning a knife-edge with an AXUV photo diode.The fluxes of soft X-rays at 516.7 eV(2.4 nm) were about 5.4×104 photons/s.100mA measured with the photo diode.The absorbed dose rate for typical yeast cells was about 11.34 Gy/s with the storage current of 100 mA.A preliminary experiment for yeast cells irradiation has shown that the microprobe had a definite biological effect for radiobiological investigations.The soft X-ray microprobe at "water window" region has provided a useful tool for single cell irradiating damage and a capability of individually irradiating a certain numbers of cells each time.展开更多
Kerman region where Sarcheshmeh zone is located includes the end part of Uremia-Dokhtar Zone. Extrusive igneous group in Sarcheshmeh is as follows: 1) andesite and 2) rhyodacite. The group of intrusive igneous rocks i...Kerman region where Sarcheshmeh zone is located includes the end part of Uremia-Dokhtar Zone. Extrusive igneous group in Sarcheshmeh is as follows: 1) andesite and 2) rhyodacite. The group of intrusive igneous rocks includes 1) porphyry granite, 2) porphyry granodiorite, and 3) porphyry diorite. Nochoon mine is located at 4 km of southwest of Sarcheshmeh copper mine and it comprises of a group of extrusive igneous rocks as follows: 1) andesite, and 2) dacite. Based on results of microprobe analysis, pyroxene mineral’s composition in these rocks of diopside and augite indicates amphiboles of composite zone of magnesium hosting type and existing plagioclases in regional rock for labradorite and bitonit composition. The most common compounds are chlorite with formula among antigorite and amosite. Comparison of magnetite mineralization in Sarcheshmeh and Nochoon indicates vein mineralization in Nochoon and disseminated granulation in Sarcheshmeh and particularly concerning to chalcopyrite, Nochoon includes more veins than Sarcheshmeh.展开更多
The oxide contents of TiO2, MnO, SrO and Fe2O3 in the body and graze layers of the Jiao-Tan-Xia (JTX) and Lao-Hu-Dong (LHD) porcelains in Southern Song Dynasty (1127-1279 A.D.) have been determined using an In- ternat...The oxide contents of TiO2, MnO, SrO and Fe2O3 in the body and graze layers of the Jiao-Tan-Xia (JTX) and Lao-Hu-Dong (LHD) porcelains in Southern Song Dynasty (1127-1279 A.D.) have been determined using an In- ternational Eagle-II μ-probe EDXRF spectrometer. The results show that the contents in the body are much different from those in the graze one. Therefore, the transient thickness (TT) between the body and graze layers can be meas- ured through determination of a distance of the drift change in the chemical contents. The TT average for the JTX porcelains is 161μm, while that for the LHD porcelains is 258μm, which are consistent with a range of 0.15-0.3mm in the Ru-Yao porcelains. The different TT is related to the variances in firing temperature and raw material for manu- facturing the respective porcelains.展开更多
The electron microprobe maps the spatial distribution of elements in a rock at a sub-mineral-grain scale to provide a basis for understanding mineralization processes and to determine optimal strategies for extraction...The electron microprobe maps the spatial distribution of elements in a rock at a sub-mineral-grain scale to provide a basis for understanding mineralization processes and to determine optimal strategies for extraction of valuable target elements. Round Top Mountain (near the town of Sierra Blanca, Hudspeth County, west Texas, USA) is a peraluminous rhyolite laccolith that is homogeneously mineralized at over 500 ppm rare earths, more than 70% of which are yttrium and heavy rare earths (YHREEs). The massive deposit is exposed at the surface as a mountain some 2 km in diameter and 375 m in height. Round Top Mountain also contains Li, Be, U, Th, Nb, Ta, Ga, Rb, Cs, Sn, and F. The valuable YHREEs are hosted in yttrofluorite, which is soluble in dilute sulfuric acid. Texas Mineral Resources Corporation proposes to surface mine, crush, and heap leach the deposit. The distribution of YHREEs, and that of other trace elements, is remarkably homogeneous at outcrop drill hole scale. Here we document that YHREE mineralization appears pervasive through the rhyolite at a millimeter scale. Back scattered electron (BSE) and characteristic X-ray maps reveal the fine grain size and apparently random and dispersed spatial distribution of the yttrofluorite that hosts Round Top’s valuable YHREEs. The yttrofluorite grains do not appear to cluster at special mineralized locations, e.g., in pores or along cracks in the rhyolite. The same is apparently true of such other potentially valuable minerals as cassiterite and uranium species. These findings confirm that the distribution of YHREEs in Round Top Mountain rhyolite is homogeneous through different orders of magnitude of scale, i.e., from outcrop (as seen in the companion work in this volume) to sub-thin section. The material thus is ideal for a heap leach operation where homogeneous feedstock is crucial to consistent and economic operation. The findings also confirm and explain why mechanical separation would prove very difficult and expensive due to the astronomical number of yttrofluorite grains in even a golf-ball-size piece of Round Top rhyolite.展开更多
基金supported by the Canadian Institute of Health Research(CIHR)-Natural Sciences and Engineering Research Council(NSERC)of Canada Collaborative Health Research Projects program(Grant No.:355935)as well as by NSERC through the Industrial Research Chair(IRC)program(Program No.:#IRCPJ 184412e15).
文摘Adjuvant chemotherapy improves the survival outlook for patients undergoing operations for lung metastases caused by colorectal cancer (CRC). However, a multidisciplinary approach that evaluates several factors related to patient and tumor characteristics is necessary for managing chemotherapy treatment in metastatic CRC patients with lung disease, as such factors dictate the timing and drug regimen, which may affect treatment response and prognosis. In this study, we explore the potential of spatial metabolomics for evaluating metabolic phenotypes and therapy outcomes during the local delivery of the anticancer drug, oxaliplatin, to the lung. 12 male Yorkshire pigs underwent a 3 h left lung in vivo lung perfusion (IVLP) with various doses of oxaliplatin (7.5, 10, 20, 40, and 80 mg/L), which were administered to the perfusion circuit reservoir as a bolus. Biocompatible solid-phase microextraction (SPME) microprobes were combined with global metabolite profiling to obtain spatiotemporal information about the activity of the drug, determine toxic doses that exceed therapeutic efficacy, and conduct a mechanistic exploration of associated lung injury. Mild and subclinical lung injury was observed at 40 mg/L of oxaliplatin, and significant compromise of the hemodynamic lung function was found at 80 mg/L. This result was associated with massive alterations in metabolic patterns of lung tissue and perfusate, resulting in a total of 139 discriminant compounds. Uncontrolled inflammatory response, abnormalities in energy metabolism, and mitochondrial dysfunction next to accelerated kynurenine and aldosterone production were recognized as distinct features of dysregulated metabolipidome. Spatial pharmacometabolomics may be a promising tool for identifying pathological responses to chemotherapy.
基金supported by a Youth Foundation from the former Ministry of GeologyMineral Resources and an 0utstanding Youth Foundation from the National Natural Science Foundation of China(grant 40425014).
文摘A suite of continental flood basalts sampled over a vast exposure and stratigraphic thickness in the Emeishan large igneous province （LIP）, SW China was investigated for laser microprobe ^40Ar/^39Ar dating. There are two ^40Ar/^39Ar age groups for these basalts, corresponding to 259-246 Ma and 177-137 Ma, respectively. A well-defined isochron gives an eruption age of huge quantities of mafic magmas at 258.9±3.4 Ma, which is identical to previous dating and paleontological data. Much younger ^40Ar/^39Ar ages for some basalts with Iow-greenschist metamorphic facies probably recorded a late thermo-tectonic event caused by collision between the Yangtze and Qiangtang continental blocks during the Mesozoic, which resulted in the reset of argon isotope system. The ^40Ar/^39Ar age data, we present here, combined with previous dating and paleontological data, suggest relatively short duration （about 3 Ma） of mafic volcanism, which have important implication on mantle plume genesis of the Emeishan continental flood basalts in the LIP.
基金This work was financially supported by the key project for international collaboration, the National Natural Science Foundation of China (Project No. 40420120135).
文摘The Longquanguan shear zone is an important structural belt in the North China Craton, separating the underlying Fuping complex from the overlying Wutai complex. This shear zone has experienced three episodes of deformation: the first and main episode is a ductile top-to-ESE shear along the gently northwest-west dipping foliations, while the other two episodes are later collapse sliding. Prolonged granites parallel to the shear foliations make one of the main compositions of the Longquanguan shear zone. These granites experienced deformation to form mylonitic rocks when they emplaced during the first episode of deformation. Structural characters of the granites and their contacts to the country rocks indicate that these granites possibly resulted from in-situ partial remelting by shearing, i.e., they are syn-deformational granites. Monazites in these mylonitic granites are magmatic minerals and their crystallization ages may represent ages of the magmatic events, and also the ages for the main deformation of the Longquanguan shear zone. Monazite electronic microprobe dating were carried on two samples of granite, which gives multiple peak ages, among which 1,846 Ma and 1,877 Ma are the main peak ages for the two samples. These ages represent the main deformation of the Longquanguan shear zone, which is consistent with the main regional geological event at about 1,850 Ma caused by the collision between the Eastern and Western Blocks in North China. The good match between the monazite ages and the corresponding regional tectono-thermal events shows the feasibility and reliability of monazite electronic microprobe dating.
文摘A combination of NAA and micro-PIXE was used to study concentrations and distributions of platinum group elements (PGE) in ores from Xinjie Cu-Pt deposit.The NAA results of the bulk indicate that the ores belong to the enriched Pt-Pd type.The element concentration maps of scanning micro-PIXE for the ores show that the occurence form of Pt is independent arsenide minerals. No PGE were detected in chalcopyrite of Xinjie Cu-Pt deposit. These information are economically beneficial to the mineral smelting process.
基金The Project Supported by the Foundation of Academic Sinica
文摘This paper describes the scanning nuclear microprobe facilities established at the Shanghai Institute of Nuclear Research. The Russion quadruplet constructed with four magnetic quadrupolets is used for microbeam formation. The long focus of the lens makes the working distance long enough to accommodate the scanning coils and the detectors for PIXE, RBS, RFS, NRA and SE experiments. A 5 μm focussed beam is scanned continously by a pair of coils. A multiparameter multidetector data system for the nuclear microprobe is based on the idea of Total quantitative scanning analysis (TQSA) suggested by Melbourne University. A digital graphic displayer is a good substitute for an ordinary storage oscilloscope for on- line scanned area monitoring. The new microprobe meets high demands of biologists and geologists on applications.
文摘In order to develop new reference materials for microanalytical nuclear techniques, scanning proton microprobe (SPM) technique was used to determine homogeneity level within 100×200 μm 2 micro area on the small pieces of IAEA urban dust reference materials. The experimental methods were described in detail. The results show that IAEA 396A/M Vienna urban dust is homogeneous enough for small sample analysis of standard reference material (SRM).
基金This project is supported by National Natural Science Foundation of China (No. 59773024).
文摘The morphology of polyvinyl chloride/polystyrene (PVC/PS) blend samples with different mass ratios, prepared by means of solution casting and melt mixing, have been successfully examined by electron microprobe analysis (EMP). This experiment was performed in a scanning electron microscope attached to an energy dispersive X-ray analyzer. Differential scanning calorimetry was also used to investigate the phase separation of the blends. The results show that PVC and PS are incompatible and the blends have sea-islands phase structures. Blends prepared via melt mixing have finer phase-dispersion than those prepared via solution casting.
文摘An X-ray microprobe for radiobiological studies was developed which deliver precise doses of radiation to the selected individual cells.The facility used synchrotron radiation as soft X-ray source.A zone plate combining with a pinhole produced a fine probe from bending magnet for single cell irradiating with defined doses.The diameter of microprobe at the target position was about 2 μm by scanning a knife-edge with an AXUV photo diode.The fluxes of soft X-rays at 516.7 eV(2.4 nm) were about 5.4×104 photons/s.100mA measured with the photo diode.The absorbed dose rate for typical yeast cells was about 11.34 Gy/s with the storage current of 100 mA.A preliminary experiment for yeast cells irradiation has shown that the microprobe had a definite biological effect for radiobiological investigations.The soft X-ray microprobe at "water window" region has provided a useful tool for single cell irradiating damage and a capability of individually irradiating a certain numbers of cells each time.
文摘Kerman region where Sarcheshmeh zone is located includes the end part of Uremia-Dokhtar Zone. Extrusive igneous group in Sarcheshmeh is as follows: 1) andesite and 2) rhyodacite. The group of intrusive igneous rocks includes 1) porphyry granite, 2) porphyry granodiorite, and 3) porphyry diorite. Nochoon mine is located at 4 km of southwest of Sarcheshmeh copper mine and it comprises of a group of extrusive igneous rocks as follows: 1) andesite, and 2) dacite. Based on results of microprobe analysis, pyroxene mineral’s composition in these rocks of diopside and augite indicates amphiboles of composite zone of magnesium hosting type and existing plagioclases in regional rock for labradorite and bitonit composition. The most common compounds are chlorite with formula among antigorite and amosite. Comparison of magnetite mineralization in Sarcheshmeh and Nochoon indicates vein mineralization in Nochoon and disseminated granulation in Sarcheshmeh and particularly concerning to chalcopyrite, Nochoon includes more veins than Sarcheshmeh.
基金Supported by the City University of Hong Kong Foundation (No.9010007) the Innovation Project Funds of CAS (Nos. KZCX2-SW-118+1 种基金 KZCX3-SW-120) the NKPBR Project Fund (No.2001CCB00100) and the SKLLQG0324 Foundation.
文摘The oxide contents of TiO2, MnO, SrO and Fe2O3 in the body and graze layers of the Jiao-Tan-Xia (JTX) and Lao-Hu-Dong (LHD) porcelains in Southern Song Dynasty (1127-1279 A.D.) have been determined using an In- ternational Eagle-II μ-probe EDXRF spectrometer. The results show that the contents in the body are much different from those in the graze one. Therefore, the transient thickness (TT) between the body and graze layers can be meas- ured through determination of a distance of the drift change in the chemical contents. The TT average for the JTX porcelains is 161μm, while that for the LHD porcelains is 258μm, which are consistent with a range of 0.15-0.3mm in the Ru-Yao porcelains. The different TT is related to the variances in firing temperature and raw material for manu- facturing the respective porcelains.
文摘The electron microprobe maps the spatial distribution of elements in a rock at a sub-mineral-grain scale to provide a basis for understanding mineralization processes and to determine optimal strategies for extraction of valuable target elements. Round Top Mountain (near the town of Sierra Blanca, Hudspeth County, west Texas, USA) is a peraluminous rhyolite laccolith that is homogeneously mineralized at over 500 ppm rare earths, more than 70% of which are yttrium and heavy rare earths (YHREEs). The massive deposit is exposed at the surface as a mountain some 2 km in diameter and 375 m in height. Round Top Mountain also contains Li, Be, U, Th, Nb, Ta, Ga, Rb, Cs, Sn, and F. The valuable YHREEs are hosted in yttrofluorite, which is soluble in dilute sulfuric acid. Texas Mineral Resources Corporation proposes to surface mine, crush, and heap leach the deposit. The distribution of YHREEs, and that of other trace elements, is remarkably homogeneous at outcrop drill hole scale. Here we document that YHREE mineralization appears pervasive through the rhyolite at a millimeter scale. Back scattered electron (BSE) and characteristic X-ray maps reveal the fine grain size and apparently random and dispersed spatial distribution of the yttrofluorite that hosts Round Top’s valuable YHREEs. The yttrofluorite grains do not appear to cluster at special mineralized locations, e.g., in pores or along cracks in the rhyolite. The same is apparently true of such other potentially valuable minerals as cassiterite and uranium species. These findings confirm that the distribution of YHREEs in Round Top Mountain rhyolite is homogeneous through different orders of magnitude of scale, i.e., from outcrop (as seen in the companion work in this volume) to sub-thin section. The material thus is ideal for a heap leach operation where homogeneous feedstock is crucial to consistent and economic operation. The findings also confirm and explain why mechanical separation would prove very difficult and expensive due to the astronomical number of yttrofluorite grains in even a golf-ball-size piece of Round Top rhyolite.
