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纳米Al_(2)O_(3)改性沥青的制备及其性能研究 被引量:1
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作者 王苗 邢红梅 张晓炜 《城市道桥与防洪》 2024年第3期240-244,M0019,共6页
为了研究纳米Al_(2)O_(3)改性剂对A-70#基质沥青物理性能、流变特性的影响及其微观改性机理。基于室内试验制备了不同掺量(1%、2%、3%、4%、5%)的纳米Al_(2)O_(3)改性沥青,对其进行三大指标、黏度、流变、抗老化性能及其傅里叶红外光谱... 为了研究纳米Al_(2)O_(3)改性剂对A-70#基质沥青物理性能、流变特性的影响及其微观改性机理。基于室内试验制备了不同掺量(1%、2%、3%、4%、5%)的纳米Al_(2)O_(3)改性沥青,对其进行三大指标、黏度、流变、抗老化性能及其傅里叶红外光谱试验测试。结果表明:加入纳米Al_(2)O_(3)之后,基质沥青的软化点和黏度提高,针入度及延度下降,纳米Al_(2)O_(3)最佳掺量为4%。由流变试验结果可知,纳米Al_(2)O_(3)可以提升基质沥青的车辙因子,显著增强其高温稳定性,抗老化性能得以提升,但对低温抗裂性能不利。根据微观分析结果,纳米Al_(2)O_(3)与基质沥青之间既产生了物理变化,又有微弱的化学反应存在。 展开更多
关键词 纳米Al_(2)O_(3) 改性沥青 物理性能 流变特性 微观机理
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Al_(2)O_(3)/SiO_(2)/ZrO_(2)三元复合气凝胶材料的制备及性能研究
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作者 徐长伟 杨鑫 +1 位作者 张忠伦 王明铭 《化工新型材料》 CAS CSCD 北大核心 2024年第10期101-107,共7页
以六水合氯化铝(AlCl_(3)·6H_(2)O)、正硅酸乙酯(TEOS)和八水合氧氯化锆(ZrOCl_(2)·8H_(2)O)为混合前驱体,以环氧丙烷(PO)作为凝胶网络诱导剂,乙醇和水作为溶剂,采用溶胶-凝胶工艺和二氧化碳超临界干燥技术制备出了Al_(2)O_(3... 以六水合氯化铝(AlCl_(3)·6H_(2)O)、正硅酸乙酯(TEOS)和八水合氧氯化锆(ZrOCl_(2)·8H_(2)O)为混合前驱体,以环氧丙烷(PO)作为凝胶网络诱导剂,乙醇和水作为溶剂,采用溶胶-凝胶工艺和二氧化碳超临界干燥技术制备出了Al_(2)O_(3)-SiO_(2)-ZrO_(2)三元复合气凝胶。研究了不同PO添加量、醇水体积比和Al/Si/Zr摩尔比对其性能和结构的影响,采用扫描电镜、比表面积分析仪和多功能粉体物理特性测试仪对气凝胶的凝胶形态、微观形貌及孔结构进行表征。结果表明:当PO添加量为88mL,醇水体积比为8∶9,Al/Si/Zr摩尔比为12∶3∶1和12∶4∶1时制备出的气凝胶结构性能良好,且凝胶时间在较合适的范围内,具有较低的密度为0.125g/cm^(3)和0.143g/cm^(3),较高的比表面积为683.14m^(2)/g和684.56m^(2)/g。 展开更多
关键词 硅铝锆复合气凝胶 凝胶时间 微观形貌 孔结构
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Quantitative determination of PFC3D microscopic parameters 被引量:5
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作者 LI Zhuo RAO Qiu-hua 《Journal of Central South University》 SCIE EI CAS CSCD 2021年第3期911-925,共15页
It is important to calibrate micro-parameters for applying partied flow code(PFC)to study mechanical characteristics and failure mechanism of rock materials.Uniform design method is firstly adopted to determine the mi... It is important to calibrate micro-parameters for applying partied flow code(PFC)to study mechanical characteristics and failure mechanism of rock materials.Uniform design method is firstly adopted to determine the microscopic parameters of parallel-bonded particle model for three-dimensional discrete element particle flow code(PFC3D).Variation ranges of microscopic of the microscopic parameters are created by analyzing the effects of microscopic parameters on macroscopic parameters(elastic modulus E,Poisson ratio v,uniaxial compressive strengthσc,and ratio of crack initial stress to uniaxial compressive strengthσci/σc)in order to obtain the actual uniform design talbe.The calculation equations of the microscopic and macroscopic parameters of rock materials can be established by the actual uniform design table and the regression analysis and thus the PFC3D microscopic parameters can be quantitatively determined.The PFC3D simulated results of the intact and pre-cracked rock specimens under uniaxial and triaxial compressions(including the macroscopic mechanical parameters,stress−strain curves and failure process)are in good agreement with experimental results,which can prove the validity of the calculation equations of microscopic and macroscopic parameters. 展开更多
关键词 quantitative relationship of microscopic and macroscopic parameters uniform design method three-dimensional particle flow code(PFC3D) ROCK
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Microscopic phase-field modeling of atomic anti-site behaviors in precipitation progress of Ni_3(AlFe) 被引量:1
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作者 梁敏洁 廖海洪 +1 位作者 丁文江 陈铮 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2010年第10期1963-1967,共5页
The effects of temperature on atomic anti-site behaviors in L12-Ni3(AlFe) phases were studied using microscopic phase-field dynamic model in precipitation progress of Ni75Al20Fe5 alloy.The results show that with the i... The effects of temperature on atomic anti-site behaviors in L12-Ni3(AlFe) phases were studied using microscopic phase-field dynamic model in precipitation progress of Ni75Al20Fe5 alloy.The results show that with the increase of temperature,the formation of NiAl and AlNi anti-sites is much easier in Ni3(AlFe),and Ni and Al anti-site atoms show clearly stronger temperature-dependent than Fe anti-site atoms.The evolution progress of anti-site atoms is completed at the initial growth stage of L12-Ni3(AlFe) phases.The site occupation probabilities of Ni atoms on the sublattice A(NiNi,face centers sites of FCC),and Al and Fe atoms on the sublattice B(AlAl and FeAl,corners sites of FCC) all present the degressive tendency with the temperature increasing.Fe atoms mainly prefer to occupy the Al sublattice at the whole temperature range. 展开更多
关键词 anti-site microscopic phase-field PRECIPITATION Ni3(AlFe)
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Electron Microscopic In-situ Observation for Preparation of Ultrafine La_2O_3 Nanophase Powder 被引量:1
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作者 高愈尊 李永洪 张泰宋 《Journal of Rare Earths》 SCIE EI CAS CSCD 1996年第3期206-209,共4页
In-situ observation in high voltage electron microscope(HVEM) was used to investigate transformation from amorphous La(OH)3 to nanophase La2O3. Electron diffraction pattern indicates that the transition temperature fr... In-situ observation in high voltage electron microscope(HVEM) was used to investigate transformation from amorphous La(OH)3 to nanophase La2O3. Electron diffraction pattern indicates that the transition temperature from La(OH)3 to nanophase La2O3 is 400 ℃. Particle sizes for nanophase La2O3 are from 20 to 50 nm. 展开更多
关键词 La_2O_3 NANOPHASE Insitu observation High voltage electron microscope
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Microscopic Observation and Stability Study on the Fe_3s_4 Nanocrystals Synthesizedunder Thermal and Humid Conditions
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作者 GAO Shang HUANG Fei +6 位作者 SONG Dan LI Guanglu LIU Jia GAO Wenyuan LIU Qiang FENG Taiwei ZHAO Ran 《矿物学报》 CAS CSCD 北大核心 2013年第S1期26-26,共1页
Fe3S4 is important magnetic mineral that widely exists in the sediments of lakes and oceans. It can not only instruct reducing environment that contains organic matter and sulfate, but also provide paleomagnetic signa... Fe3S4 is important magnetic mineral that widely exists in the sediments of lakes and oceans. It can not only instruct reducing environment that contains organic matter and sulfate, but also provide paleomagnetic signal for paleoenvironmet research. Simultaneously, as a new type of magnetic material, it causes attention. Because Fe3S4 generally exists as an unstable intermediate, it is stringent in preparation conditions. Although some scholars have conducted on the synthesis experiments of Fe3S4 materials, the research on its stable conditions, formation mechanism and evolution process is not yet depth. Accordingly, defining the stable conditions and revealing evolution law of Fe3S4 nanocrystals have important significance for the determination of environmental conditions and the preparation of pure Fe3S4 nanomaterials. 展开更多
关键词 microscopIC OBSERVATION STABILITY Fe3S4 THERMAL and humid CONDITIONS
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Microscopic Theory of the Thermodynamic Properties of Sr_3Ru_2O_7
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作者 李伟正 吴从军 《Chinese Physics Letters》 SCIE CAS CSCD 2016年第3期86-90,共5页
The thermodynamic properties of the bilayer ruthenate compound Sr3Ru2O7 at very low temperatures are inves- tigated by using a tight-binding model yielding the realistic band structure combined with the on-site intera... The thermodynamic properties of the bilayer ruthenate compound Sr3Ru2O7 at very low temperatures are inves- tigated by using a tight-binding model yielding the realistic band structure combined with the on-site interactions treated at the mean-field level. We find that both the total density of states at the Fermi energy and the entropy exhibit a sudden increase near the critical magnetic field for the nematic phase, echoing the experimental find- ings. A new mechanism to explain the anisotropic transport properties is proposed based on scatterings at the anisotropic domain boundaries. Our results suggest that extra cares are necessary to isolate the contributions due to the quantum criticality from the band structure singularity in Sr3Ru2O7. 展开更多
关键词 in as on IT of RU microscopic Theory of the Thermodynamic Properties of Sr3Ru2O7 SR IS that
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ELECTRON MICROSCOPIC OBSERVATION OF SIMIAN SARCOMA VIRUS TRANSFORMED NIH 3T3 CELLS IN VITRO
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作者 田竟生 苏树芸 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1989年第2期16-18,共3页
For electronic microscopic observation, we found SSV-transformed NIH 3T3 cells were different from non-transformed cells. In SSV-transformed NIH 3T3 cells nuclei cytoplasma ratio was increased and in cytoplasma the ri... For electronic microscopic observation, we found SSV-transformed NIH 3T3 cells were different from non-transformed cells. In SSV-transformed NIH 3T3 cells nuclei cytoplasma ratio was increased and in cytoplasma the ribosomes (polyribosomes were attached to the swollen rough endoplasmic reticulum. It was likely that ribosomes were lined together functionally and structionally to produce specific protein (PDGF-like protein). 展开更多
关键词 NIH ELECTRON microscopIC OBSERVATION OF SIMIAN SARCOMA VIRUS TRANSFORMED NIH 3T3 CELLS IN VITRO RER
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Studies on mitochondrial macromolecular syntheses in various organs of aging animals labeled with 3H-precursors as revealed by electron microscopic radioautography
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作者 Tetsuji Nagata 《Advances in Bioscience and Biotechnology》 2010年第4期241-262,共22页
In order to study the aging changes of intramitochondrial macromolecular synthesis in various organs of aging animals during the development and aging, 10 groups of developing and aging mice, each consisting of 3 indi... In order to study the aging changes of intramitochondrial macromolecular synthesis in various organs of aging animals during the development and aging, 10 groups of developing and aging mice, each consisting of 3 individuals, total 30, from fetal day 19 to postnatal newborn at day 1, 3, 9, 14 and adult at month 1, 2, 6, 12 to 24 were injected with 3H-thymidine a DNA precurson, another 10 groups consisting of 3 individuals, total 30, were injected with 3H-uridine a RNA precursor, and another 10 groups of 30 individuals were injected with 3H-leucine a protein precursor, total 90 individuals. Then, all the animals were sacrificed 1 hr after the injections and the liver tissues, the lung tissues, the kidney tissues, the testis and ovary tissues, the adrenal tissues were taken out, fixed and processed for electron microscopic radioautography. On electron microscopic radioautograms obtained from each animal, ten photographs in respective groups, numbers of mitochondria per cell profile area, numbers of labeled mitochondria per cell and the mitochondrial labeling index (LI) labeled with 3H-thymidine showing DNA synthesis, LI labeled with 3H-uridine showing RNA synthesis, and LI labeled with 3H-leucine showing protein synthesis, in various organs, were counted and the results in various organs in respective aging groups were compared, respectively. From the results, it was demonstrated that the numbers of mitochondria in hepatocytes as well as in 3 zones of the adrenal cortex, the zona glomerulosa, fasciculata and reticularis of mice at various ages increased from fetal day 19 to postnatal month 1 due to development and aging of animals. On the other hand, the numbers of labeled mitochondria and the labeling indices of intramitochondrial DNA syntheses incorporating 3H-thymidine in hepatocytes and 3 zones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. The numbers of labeled mitochondria and the labeling indices of intramitochondrial RNA syntheses incorporating 3H-uridine in hepatocytes and 3 zones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. The numbers of labeled mitochondria and the labeling indices of intramitochondrial RNA syntheses incorporating 3H-uridine in hepatocytes and 3 ones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. Moreover, some other organs such as the lung and the testis were also review and discussed. From the results, it was shown that the activity of intramitochnodrial DNA synthesis, RNA synthesis, and protein synthesis in hepatocytes, adrenal cortical cells, pulmonary cells and testicular cells in developing and aging mice as well as some other organs increased and decreased due to development and aging. 展开更多
关键词 MITOCHONDRIAL Macromolecular SYNTHESES AGING ANIMALS Labeled 3H-Precursors ELECTRON microscopIC Radioautography
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超音速激光沉积Cu-Al_(2)O_(3)-石墨复合涂层微观结构及耐磨损性能
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作者 孙启帆 林钟卫 +3 位作者 刘博 章钢 李波 姚建华 《表面技术》 EI CAS CSCD 北大核心 2024年第5期115-125,共11页
目的研究不同石墨含量对超音速激光沉积Cu-Al_(2)O_(3)-石墨复合涂层的微观组织、显微硬度、耐磨损性能的影响。方法利用扫描电子显微镜、能量色谱仪、维氏硬度计、激光共聚焦扫描显微系统、X射线衍射仪、摩擦磨损测试对复合涂层的微观... 目的研究不同石墨含量对超音速激光沉积Cu-Al_(2)O_(3)-石墨复合涂层的微观组织、显微硬度、耐磨损性能的影响。方法利用扫描电子显微镜、能量色谱仪、维氏硬度计、激光共聚焦扫描显微系统、X射线衍射仪、摩擦磨损测试对复合涂层的微观组织、显微硬度、耐磨损性能及磨损机制进行分析。结果随着原始粉末中镀铜石墨质量占比的增加,Cu-Al_(2)O_(3)-石墨复合涂层的沉积效率逐渐降低。基于Al_(2)O_(3)颗粒的原位喷丸效应及激光辐照的加热软化效应,复合涂层具有致密的微观组织,且复合涂层与基体界面结合良好。单一添加Al_(2)O_(3)颗粒可以将Cu涂层的硬度从108.19HV0.2提高至121.82HV0.2。随着石墨含量的增大,涂层的显微硬度逐渐降低,镀铜石墨在原始粉末中的质量分数从5%增至15%,Cu-Al_(2)O_(3)-石墨复合涂层的硬度从116.09HV0.2降至94.17HV0.2。添加石墨能够在复合涂层表面形成固体润滑层,降低复合涂层的摩擦因数,提升涂层的耐磨损性能。CuAlGr10复合涂层具有最优的耐磨损性能,磨损率为0.7×10^(−4)mm^(3)/(N·m)。此外,由于激光辐照促进了复合涂层内部颗粒间的界面结合,均匀分散在石墨润滑相中的Al_(2)O_(3)颗粒作为负载支撑和耐磨相,可进一步降低复合涂层的磨损率。结论Cu-Al_(2)O_(3)-石墨复合涂层优异的耐磨性能是润滑相石墨颗粒和硬质增强相Al_(2)O_(3)颗粒共同作用的结果,石墨的添加能够降低复合涂层的摩擦因数,提升涂层的耐磨损性能,但过量的石墨颗粒会对涂层产生割裂作用,导致增强相Al_(2)O_(3)颗粒脱离涂层,从而加剧涂层的磨损。 展开更多
关键词 超音速激光沉积 Cu-Al_(2)O_(3)-石墨复合涂层 微观组织 显微硬度 耐磨损性能 磨损机制
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低温环境下PbTiO_(3)钙钛矿晶粒的演化过程
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作者 于海涛 陈国新 +2 位作者 吴海辰 梁芮 卢焕明 《理化检验(物理分册)》 CAS 2024年第5期1-3,共3页
利用透射电子显微镜研究了PbTiO_(3)钙钛矿在低温条件下的晶体结构及晶粒演化过程。结果表明:低温条件下,PbTiO_(3)钙钛矿未出现明显的相转变;在电子束辐照条件下,PbTiO_(3)晶体出现细晶化现象,说明电子束对其结构变化存在诱导作用;在... 利用透射电子显微镜研究了PbTiO_(3)钙钛矿在低温条件下的晶体结构及晶粒演化过程。结果表明:低温条件下,PbTiO_(3)钙钛矿未出现明显的相转变;在电子束辐照条件下,PbTiO_(3)晶体出现细晶化现象,说明电子束对其结构变化存在诱导作用;在室温和低温环境下,PbTiO_(3)晶体均未产生位错缺陷,且低温环境使晶粒细化过程变慢;电子辐照作用使材料的表面能变大,导致晶粒尺寸变小,从而出现细晶化现象。 展开更多
关键词 PbTiO_(3)钙钛矿 透射电子显微镜 细晶化现象 相变 表面能
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Acoustical properties of a 3D printed honeycomb structure filled with nanofillers:Experimental analysis and optimization for emerging applications
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作者 Jeyanthi Subramanian Vinoth kumar Selvaraj +3 位作者 Rohan Singh Ilangovan S Naresh Kakur Ruban Whenish 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第5期248-258,共11页
The novelty of this research lies in the successful fabrication of a 3D-printed honeycomb structure filled with nanofillers for acoustic properties,utilizing an impedance tube setup in accordance with ASTM standard E ... The novelty of this research lies in the successful fabrication of a 3D-printed honeycomb structure filled with nanofillers for acoustic properties,utilizing an impedance tube setup in accordance with ASTM standard E 1050-12.The Creality Ender-3,a 3D printer,was used for printing the honeycomb structures,and polylactic acid(PLA)material was employed for their construction.The organic,inorganic,and polymeric compounds within the composites were identified using fourier transformation infrared(FTIR)spectroscopy.The structure and homogeneity of the samples were examined using a field emission scanning electron microscope(FESEM).To determine the sound absorption coefficient of the 3D printed honeycomb structure,numerous samples were systematically developed using central composite design(CCD)and analysed using response surface methodology(RSM).The RSM mathematical model was established to predict the optimum values of each factor and noise reduction coefficient(NRC).The optimum values for an NRC of 0.377 were found to be 1.116 wt% carbon black,1.025 wt% aluminium powder,and 3.151 mm distance between parallel edges.Overall,the results demonstrate that a 3Dprinted honeycomb structure filled with nanofillers is an excellent material that can be utilized in various fields,including defence and aviation,where lightweight and acoustic properties are of great importance. 展开更多
关键词 3D printing Honeycomb structure ACOUSTICS Field emission scanning electron microscope Response surface methodology
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GPa级高压对Mg-3Al合金凝固组织及性能的影响
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作者 代鹏林 华永乐 +2 位作者 阔洋 叶杰 林小娉 《热加工工艺》 北大核心 2024年第9期80-83,共4页
利用XRD、SEM、EDS研究了Mg-3Al合金在石墨型铸造(5℃水冷,1 atm(1.01325×105Pa))和高压凝固条件下(2、3 GPa)的凝固组织及性能。结果表明,Mg-3Al合金在不同凝固压力下均由α-Mg组成,但其组织形貌由石墨型铸造的等轴状转变为高压... 利用XRD、SEM、EDS研究了Mg-3Al合金在石墨型铸造(5℃水冷,1 atm(1.01325×105Pa))和高压凝固条件下(2、3 GPa)的凝固组织及性能。结果表明,Mg-3Al合金在不同凝固压力下均由α-Mg组成,但其组织形貌由石墨型铸造的等轴状转变为高压下的枝晶团状;GPa级高压能使Mg-3Al合金中更多的Al溶质固溶到α-Mg基体,同时也可以溶质原子的形式存在于枝晶间和晶界处发生偏聚现象。在3 GPa高压下Mg-3Al合金的抗压强度达到372 MPa,硬度达到46 HV,两者显著高于石墨型铸造Mg-3Al合金的356 MPa和41 HV。 展开更多
关键词 Mg-3Al合金 显微组织 高压凝固 力学性能
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Fe_(2)O_(3)对建窑黄兔毫釉纹路及结构的影响
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作者 方圆 李豪 +7 位作者 江财水 包启富 刘昆 陈涛升 于欢 徐轶男 程智鹏 周健儿 《中国陶瓷》 CAS CSCD 北大核心 2024年第7期66-73,共8页
以建阳当地原料红土、建瓯石、草木灰等为主要原料,研究不同Fe_(2)O_(3)含量对建窑兔毫釉黄色纹路及显微结构的影响规律,解析毫纹的变化机理。实验结果表明:Fe_(2)O_(3)含量影响着釉中ε-Fe_(2)O_(3)晶体析出的数量、形态及尺寸,使得毫... 以建阳当地原料红土、建瓯石、草木灰等为主要原料,研究不同Fe_(2)O_(3)含量对建窑兔毫釉黄色纹路及显微结构的影响规律,解析毫纹的变化机理。实验结果表明:Fe_(2)O_(3)含量影响着釉中ε-Fe_(2)O_(3)晶体析出的数量、形态及尺寸,使得毫纹呈现不同形态。Fe_(2)O_(3)含量为0.11 mol,釉中主要为玻璃相,析出晶体较少,形态为不规则块状,尺寸约50~100 nm,釉面呈灰青色而未出现毫纹。Fe_(2)O_(3)含量增至0.13~0.15 mol,ε-Fe_(2)O_(3)晶体析出较多,形态为短柱状,尺寸约50~150 nm,釉面形成细短蓝白色毫纹。当Fe_(2)O_(3)含量增至0.17~0.19 mol,ε-Fe_(2)O_(3)晶体发育形成单层和多层枝状晶花,尺寸约250~450 nm,釉面形成较长拉丝状黄色兔毫纹。Fe_(2)O_(3)含量增至0.21~0.23 mol,ε-Fe_(2)O_(3)晶体发育成树叶状大晶花,尺寸约1~2μm,釉面形成柿红。釉中Fe_(2)O_(3)含量处于0.15~0.21 mol,釉面形成的黄色毫纹数量适中,形态纤细,呈较好的拉丝状。 展开更多
关键词 建窑黄兔毫 Fe_(2)O_(3)含量 ε-Fe_(2)O_(3)晶体 毫纹形态 显微结构
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Caspase-3参与调控蟾酥诱导人肺腺癌(ASTC-a-1)细胞的凋亡 被引量:11
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作者 陈同生 王小平 +2 位作者 孙磊 王会营 王龙祥 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2008年第1期85-90,共6页
采用基因质粒转染技术、荧光发射谱检测分析以及荧光共振能量转移(FRET)受体光漂白技术,首次在活细胞中实时检测中药蟾酥(Chan-Su,CS或bufonis venenum)诱导人肺腺癌(ASTC-a-1)细胞凋亡过程中caspase-3的活化特性.采用CCK-8(Cell Counei... 采用基因质粒转染技术、荧光发射谱检测分析以及荧光共振能量转移(FRET)受体光漂白技术,首次在活细胞中实时检测中药蟾酥(Chan-Su,CS或bufonis venenum)诱导人肺腺癌(ASTC-a-1)细胞凋亡过程中caspase-3的活化特性.采用CCK-8(Cell Couneing Kit-8)技术检测发现,蟾酥对细胞的活性具有显著的抑制作用;蟾酥处理稳定表达FRET质粒SCAT3的人肺腺癌细胞后,在不同的时间检测活细胞中SCAT3的荧光光谱;利用共聚焦扫描荧光显微成像技术检测蟾酥处理后细胞的形态,从而进一步证实蟾酥诱导细胞凋亡.实验结果表明:a.蟾酥可以有效抑制人肺腺癌(ASTC-a-1)细胞的增殖活性并诱导细胞的死亡.蟾酥对细胞的抑制作用具有剂量依赖性;b.蟾酥处理细胞6h后能检测到明显的细胞凋亡小体,连续作用24h后细胞全部皱褶,并有部分细胞破碎;c.蟾酥作用细胞2h就能明显切割细胞内的SCAT3,细胞内SCAT3的切割程度随着蟾酥作用时间的延长而增加,24h内细胞内的SCAT3完全被切割.受体光漂白实验也证实了该结论,表明caspase-3参与调控了蟾酥诱导的细胞凋亡过程. 展开更多
关键词 蟾酥 荧光光谱 caspae-3 荧光共振能量转移(FRET) 激光共聚焦荧光显微成像
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荧光探针Fluo-3/AM和FuraRed测量单细胞中比率钙 被引量:6
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作者 马晓冬 朱晓亮 +5 位作者 赵清 张瑾 张飒 雷国华 黄行许 鲍永耀 《中国医学物理学杂志》 CSCD 1999年第3期180-182,共3页
应用ACAS570激光扫描共聚焦显微镜(laserscanningconfocalmicroscope,LSCM简称共焦显微镜)和钙离子指示剂Fluo-3/AM、FuraRed荧光探剂双标记技术,测定了单个活细胞内比率钙的动态变化。结果显示37℃,Fluo-3/AM浓度10μmol/L,... 应用ACAS570激光扫描共聚焦显微镜(laserscanningconfocalmicroscope,LSCM简称共焦显微镜)和钙离子指示剂Fluo-3/AM、FuraRed荧光探剂双标记技术,测定了单个活细胞内比率钙的动态变化。结果显示37℃,Fluo-3/AM浓度10μmol/L,FuraRed浓度10μmol/L的条件下,昆明小鼠巨噬细胞负载1h左右即可获良好的标记效果。用比率探针测量细胞内Ca^2+的动态变化,使Ca^2+的定性定量测定不受染料浓度、细胞大小、照射光强度以及一定程度的光漂白和染料泄漏等因素的影响,提供了一种准确定性定量细胞内Ca^2+的实时、动态、原位变化的新方法。 展开更多
关键词 激光扫描 共聚焦显微镜 比率钙 荧光探针
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消癌平诱导人肺腺癌(ASTC-a-1)细胞内caspase-3活化的荧光光谱分析 被引量:12
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作者 陈同生 王小平 +3 位作者 王治平 王龙祥 王会营 邢达 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2008年第6期1327-1331,共5页
采用CCK-8技术检测发现传统中药消癌平(XAP)对人肺腺癌(ASTC-a-1)细胞的增殖活性具有显著的抑制作用。利用共聚焦扫描荧光显微成像、荧光共振能量转移(FRET)及其受体光漂白技术证实了基于FRET技术构建的SCAT3质粒在ASTC-a-1细胞中的稳... 采用CCK-8技术检测发现传统中药消癌平(XAP)对人肺腺癌(ASTC-a-1)细胞的增殖活性具有显著的抑制作用。利用共聚焦扫描荧光显微成像、荧光共振能量转移(FRET)及其受体光漂白技术证实了基于FRET技术构建的SCAT3质粒在ASTC-a-1细胞中的稳定表达。将消癌平加入细胞培养液中培育细胞,并在不同的时间检测活细胞内SCAT3的荧光发射光谱,从而监测细胞中caspase-3的活化状态。实验结果表明:(1)消癌平可以有效抑制人肺腺癌(ASTC-a-1)细胞的增殖活性并诱导细胞的死亡,消癌平对细胞的抑制作用具有剂量依赖性。(2)消癌平处理细胞72 h后,细胞内大量的SCAT3被切割,表明细胞内caspase-3的活化水平明显升高。(3)将含消癌平的细胞培养液与细胞共同培养24 h,然后采用没有消癌平的细胞培养液培养细胞48和72 h后,细胞内SCAT3的光谱没有明显改变,表明消癌平作用细胞24 h内没有显著激活细胞内的caspase-3。 展开更多
关键词 消癌平 荧光光谱 caspae-3 荧光共振能量转移(FRET) 激光共聚焦荧光显微镜
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紫杉醇诱导不依赖于Caspase-3细胞类似Paraptosis的荧光光谱分析 被引量:6
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作者 陈同生 王小平 +2 位作者 孙磊 王会营 王龙祥 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2008年第11期2623-2627,共5页
研究了紫杉醇(Taxol)诱导人肺腺癌细胞(ASTC-a-1)类似Paraptosis的特征和机理。采用CCK-8技术检测了抑制细胞活性的特性,结果表明大于70μmol.L-1浓度的紫杉醇可以显著抑制细胞活性;采用激光共聚焦扫描荧光成像从形态上检测了紫杉醇诱... 研究了紫杉醇(Taxol)诱导人肺腺癌细胞(ASTC-a-1)类似Paraptosis的特征和机理。采用CCK-8技术检测了抑制细胞活性的特性,结果表明大于70μmol.L-1浓度的紫杉醇可以显著抑制细胞活性;采用激光共聚焦扫描荧光成像从形态上检测了紫杉醇诱导细胞死亡的形态特征,表明紫杉醇诱导了类似副凋亡(Paraptosis)特征的细胞肿胀和细胞质空泡化,而且没有细胞膜皱褶、细胞核浓缩等细胞凋亡的特征;通过基因质粒转染在细胞转染稳定表达基于荧光共振能量转移(FRET)质粒SCAT3,并利用FRET受体光漂白技术和荧光光谱检测分析技术研究了紫杉醇诱导细胞类似Paraptosis过程中Caspase-3的活化特性,结果表明在紫杉醇诱导细胞质肿胀和细胞死亡的过程中Caspase-3没有被活化。以上研究结果表明紫杉醇可以通过类似Paraptosis的方式明显诱导细胞程序性死亡,该过程不依赖于Caspase-3的活性。 展开更多
关键词 紫杉醇(Taxol) Paraptosis 荧光光谱 Caspae-3 荧光共振能量转移(FRET) 激光共聚焦荧光显微镜
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ErbB-3结合蛋白Ebp1对NIH3T3细胞生长增殖的影响 被引量:4
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作者 许东元 刘兰 +2 位作者 李良昌 金政 李光昭 《解剖学杂志》 CAS CSCD 北大核心 2008年第6期764-766,共3页
目的:探讨erbB-3结合蛋白Ebpl对NIH3T3细胞生长增殖的影响。方法:将真核表达质粒pEGFP-C1和Ebpl基因经双酶切后用T4连接酶连接,并经鉴定序列正确后,用脂质体转染剂Lipofectin Reagent稳定转染NIH3T3细胞,免疫细胞化学显色,在激光共聚焦... 目的:探讨erbB-3结合蛋白Ebpl对NIH3T3细胞生长增殖的影响。方法:将真核表达质粒pEGFP-C1和Ebpl基因经双酶切后用T4连接酶连接,并经鉴定序列正确后,用脂质体转染剂Lipofectin Reagent稳定转染NIH3T3细胞,免疫细胞化学显色,在激光共聚焦扫描显微镜下观察Ebp1蛋白的表达和定位;通过平板集落形成率观察细胞生长增殖能力的改变。结果:重组质粒鉴定正确,成功构建Ebp1稳定表达的NIH3T3细胞系,并在显微镜下可见绿色荧光,免疫细胞化学显色可见Ebp1蛋白表达;转染目的基因的细胞克隆形成率明显升高。结论:成功构建Ebp1稳定表达细胞系,Ebp1融合蛋白表达于胞质,呈不均匀颗粒状、环状分布;Ebp1在体外能增强NIH3T3细胞生长增殖。 展开更多
关键词 ErbB-3结合蛋白 NIH3T3细胞 细胞增殖 激光共聚焦显微镜
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采用Fluo-3 AM-ester探针研究桃不同组织的Ca^2+信号分布 被引量:2
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作者 鲁振华 王志强 +3 位作者 彭斌 牛良 崔国朝 刘淑娥 《果树学报》 CAS CSCD 北大核心 2011年第5期915-917,I0001,共4页
钙作为植物最重要的矿质元素之一,在植物生理和生化活动中起重要作用。采用激光扫描共聚焦显微镜,以Fluo-3 AM-ester为荧光探针研究了桃果肉和叶柄组织中Ca2+的分布,结果表明Fluo-3 AM-ester可作为荧光探针对桃不同组织中游离的Ca2+信... 钙作为植物最重要的矿质元素之一,在植物生理和生化活动中起重要作用。采用激光扫描共聚焦显微镜,以Fluo-3 AM-ester为荧光探针研究了桃果肉和叶柄组织中Ca2+的分布,结果表明Fluo-3 AM-ester可作为荧光探针对桃不同组织中游离的Ca2+信号强度和分布特征进行分析。不同的物镜观察(10×和20×)下,Ca2+荧光信号相对强度差异不大;在叶柄中Ca2+荧光信号主要分布于维管束,在果实中多分布于细胞壁;但叶柄中荧光信号分布明显高于果肉组织,这与钙在不同组织中的功能有关。 展开更多
关键词 Ca2+信号 激光扫描共聚焦显微镜 Fluo-3AM-ester
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