Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as zebrafish.However,the sample adsorption and scat...Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as zebrafish.However,the sample adsorption and scattering cause shading of the light-sheet illumination,preventing the even 3D image of thick samples.Herein,we report a continuous-rotational light-sheet microscope(CR-LSM)that enables simultaneous 3D bright-field and fluorescence imaging.With a high-accuracy rotational stage,CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation angles.Then,3D morphology and fluorescent structure were reconstructed with a developed algorithm.Using CR-LSM,zebrafish’s whole-fish contour and blood vessel structures were obtained simultaneously.展开更多
ABU Robocon是由亚太广播联盟举办的针对亚太地区高校学生的国际教育活动,我校团队在相关机器人的研发中,自主开发了一套基于RTOS的STM32单片机通信发送管理系统,可以很好地解决传统发送系统在RTOS环境下出现的发送不完整和发送时间较...ABU Robocon是由亚太广播联盟举办的针对亚太地区高校学生的国际教育活动,我校团队在相关机器人的研发中,自主开发了一套基于RTOS的STM32单片机通信发送管理系统,可以很好地解决传统发送系统在RTOS环境下出现的发送不完整和发送时间较长的问题,并通过实验证明了本文所设计的系统相较传统方法的优势。本通信系统已经被应用到了ABU Robocon的备赛和比赛环节,可以实现上下位机的高效通信。展开更多
基金the National Natural Science Foundation of China(62205368)the Key Research and Development Program of Jiangsu Province(BE2020664).
文摘Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as zebrafish.However,the sample adsorption and scattering cause shading of the light-sheet illumination,preventing the even 3D image of thick samples.Herein,we report a continuous-rotational light-sheet microscope(CR-LSM)that enables simultaneous 3D bright-field and fluorescence imaging.With a high-accuracy rotational stage,CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation angles.Then,3D morphology and fluorescent structure were reconstructed with a developed algorithm.Using CR-LSM,zebrafish’s whole-fish contour and blood vessel structures were obtained simultaneously.