Temporal change of plankton size structure preserved by Lugol's solution:a FlowCAM study

Plankton size structure is crucial for understanding marine ecosystem dynamics and the associated biogeochemical processes.A fixation step by acid Lugol’s solution has been commonly employed to preserve plankton samples in the field.However,the acid Lugol’s solution can bias the estimation of size structure and the preserved plankton size structure can vary with time.Here,we explore the impact of sample storage time on the size-structure of the plankton community preserved by Lugol’s solution.Two short-term experiments and one long-term experiment were conducted to explore the change of plankton community size structure with the storage time:covering from a week to a month,and to nearly seven months based on particle-size data obtained by continuous Flow Cytometer and Microscope(FlowCAM)measurements.We found a linear change of plankton size with the storage time in short-term periods(less than 3 months)with a decrease of the slope but an increase of the intercept for the normalized biomass size spectrum(NBS S).However,there were opposite trends for NBSS with increasing slope but decreasing intercept after3 months.The potential causes of the distinct patterns of the NBSS parameters are addressed in terms of the interplay between particle aggregation and fragmentation.We found large changes in plankton biovolume and abundance among different size classes,which may indicate a distinct effect of acid Lugol’s solution on various plankton size classes.The mechanism driving temporal change in the size-structure of the Lugolfixed plankton community was further discussed in terms of particle aggregation and fragmentation.Finally,we emphasize that the effect of storage time should be taken into account when interpreting or comparing data of plankton community acquired from samples with various storage durations.

Improved STCA Model for Multi-Lane Using Driving Guidance under CVIS

In a multi-lane area,the increasing randomness of lane changes contributes to traffic insecurity and local traffic flow instability.A study on safe lane shifting activity that focuses on threat assessment under real-time knowledge is necessary to enhance smooth vehicle flow.This paper proposed amore comprehensive lane changing guidance rule to investigate the status of surrounding vehicles to accommodate future vehicle-on-road collaborative environments based on these parameters 1)lane change demand and 2)treat assessment function.The collaborative relationships between vehicles are analyzed using a cellular automata model based on their location,velocity,and acceleration.We analyze and examine the relationship between the number of lanes and traffic flow when the road capacity is heavily mined via intelligent lane changing.Our analysis can further provide theoretical guidance for the selection of road expansion mode.Our proposed STCA-L is compared based on the average speed,average flow,lane changing frequency,spatial and temporal pattern of STCA,STCA-I,and STCA-S,and STCA-M under different vehicle densities.The numerical simulation results show that our proposed STCA-L provides themost flexible lane changing guidance in the multi-lanes road.Moreover,the simulated results show that the exponential growth of physical space cannot provide the corresponding increase in the average flow of vehicles.

Thapsigargin increases apoptotic cell death in human hepatoma BEL-7404 cells

Effects of thapsigargin, an inhibitor of Ca2+-ATPase in surface of endoplasmic reticulum, on apoptotic cell death were studied in human hepatoma cells of BEL-7404 cell line by using both flow cytometry and electron mi-croscopy Propidium iodide staining and flow cytome- try revealed that in the serum-free condition, thapsigar-gin increased the rate of apoptosis of BEL- 7404 cells in a dose-dependent manner. Prolongation of the period of serum-free condition enhanced the apoptosis induced by thapsigargin treatment. Morphological observation with electron microscope further demonstrated that chromatin condensation and fragmentation, apoptotic bodies existed in TG-treated cells, supporting that thapsigargin is a po-tent activator of apoptosis in the cells.

Effect of tempering temperature on strain hardening exponent and flow stress curve of 1000MPa grade steel for construction machinery

To study the effect of tempering temperature on strain hardening exponent and flow stress curve,one kind of 1000 MPa grade low carbon bainitic steel for construction machinery was designed,and the standard uniaxial tensile tests were conducted at room temperature.A new flow stress model,which could predict the flow behavior of the tested steels at different tempering temperatures more efficiently,was established.The relationship between mobile dislocation density and strain hardening exponent was discussed based on the dislocation-stress relation.Arrhenius equation and an inverse proportional function were adopted to describe the mobile dislocation,and two mathematical models were established to describe the relationship between tempering temperature and strain hardening exponent.Nonlinear regression analysis was applied to the Arrhenius type model,hence,the activation energy was determined to be 37.6kJ/mol.Moreover,the square of correlation coefficient was 0.985,which indicated a high reliability between the fitted curve and experimental data.By comparison with the Arrhenius type curve,the general trend of the inverse proportional fitting curve was coincided with the experimental data points except of some fitting errors.Thus,the Arrhenius type model can be adopted to predict the strain hardening exponent at different tempering temperatures.

On the impact of connected automated vehicles in freeway work zones:a cooperative cellular automata model based approach

Purpose–Freeway work zones have been traffic bottlenecks that lead to a series of problems,including long travel time,high-speed variation,driver’s dissatisfaction and traffic congestion.This research aims to develop a collaborative component of connected and automated vehicles(CAVs)to alleviate negative effects caused by work zones.Design/methodology/approach–The proposed cooperative component is incorporated in a cellular automata model to examine how and to what scale CAVs can help in improving traffic operations.Findings–Simulation results show that,with the proposed component and penetration of CAVs,the average performances(travel time,safety and emission)can all be improved and the stochasticity of performances will be minimized too.Originality/value–To the best of the authors’knowledge,this is the first research that develops a cooperative mechanism of CAVs to improve work zone performance.