To explore the relationship between microsomal triglyceride transfer protein (MT P) gene variation and diabetic dyslipidemia among Chinese Methods Using PCR restriction fragment length polymorphism (PCR RFLP) anal...To explore the relationship between microsomal triglyceride transfer protein (MT P) gene variation and diabetic dyslipidemia among Chinese Methods Using PCR restriction fragment length polymorphism (PCR RFLP) analysis and gene sequencing, we studied the influence of a common MTP gene polymorphism in the p romoter region on the apoB containing lipoproteins in 44 Chinese type 2 diabeti c subjects and 32 non diabetic volunteers Results A common functional G/T polymorphism in 493 bp upstream from the transcriptional start point was detected among native Chinese There were 41 carriers (53 9%) of the MTP 493 G/G genotype, 28 (36 8%) of the MTP 493 G/T genotype and 7 (9 3%) of the MTP 493 T/T genotype The allele frequency of M TP 493 T in the diabetic group was 0 30 The MTP 493 T/T diabetic group had significantly higher TG ( P <0 05), VLDL CH ( P <0 05) and smaller LDL pa rticle size ( P <0 001) than the MTP 493 common genotype group Conclusion Genetic variation in the MTP promoter is likely to be highly involved in the pro duction of dyslipidemia in type 2 diabetic subjects展开更多
This study was performed for investigation the relationship between variants of MTP gene polymorphism and the development of NAFLD in patients with and without MS.The study was included 174 NAFLD patients(106 with MS ...This study was performed for investigation the relationship between variants of MTP gene polymorphism and the development of NAFLD in patients with and without MS.The study was included 174 NAFLD patients(106 with MS and 68 without MS),and 141 healthy control subjects.The 493 G/T polymorphism of MTP gene was evaluated by PCR-RFLP method.The frequency of MTP TT genotype and T allele were significantly higher in NAFLD patients when compared to healthy controls.Moreover,a significant association in MTP gene polymorphism was observed in NAFLD patients with MS compared to NAFLD patients without MS and controls.Our study suggested that MTP 493 G/T gene polymorphism may act as susceptibility biomarker for NAFLD and MS.展开更多
文摘To explore the relationship between microsomal triglyceride transfer protein (MT P) gene variation and diabetic dyslipidemia among Chinese Methods Using PCR restriction fragment length polymorphism (PCR RFLP) analysis and gene sequencing, we studied the influence of a common MTP gene polymorphism in the p romoter region on the apoB containing lipoproteins in 44 Chinese type 2 diabeti c subjects and 32 non diabetic volunteers Results A common functional G/T polymorphism in 493 bp upstream from the transcriptional start point was detected among native Chinese There were 41 carriers (53 9%) of the MTP 493 G/G genotype, 28 (36 8%) of the MTP 493 G/T genotype and 7 (9 3%) of the MTP 493 T/T genotype The allele frequency of M TP 493 T in the diabetic group was 0 30 The MTP 493 T/T diabetic group had significantly higher TG ( P <0 05), VLDL CH ( P <0 05) and smaller LDL pa rticle size ( P <0 001) than the MTP 493 common genotype group Conclusion Genetic variation in the MTP promoter is likely to be highly involved in the pro duction of dyslipidemia in type 2 diabetic subjects
基金This work was supported by grant from National Research Centre,Egypt(Project Number 10010205).
文摘This study was performed for investigation the relationship between variants of MTP gene polymorphism and the development of NAFLD in patients with and without MS.The study was included 174 NAFLD patients(106 with MS and 68 without MS),and 141 healthy control subjects.The 493 G/T polymorphism of MTP gene was evaluated by PCR-RFLP method.The frequency of MTP TT genotype and T allele were significantly higher in NAFLD patients when compared to healthy controls.Moreover,a significant association in MTP gene polymorphism was observed in NAFLD patients with MS compared to NAFLD patients without MS and controls.Our study suggested that MTP 493 G/T gene polymorphism may act as susceptibility biomarker for NAFLD and MS.
