The serum levels of IL-12 and IL-10 in mice after infected with Babesia microti （B. microti） and Babesia rodhaini （B. rodhaini） were examined. Collected the mice serum and examined the concentration of IL-12 and I...The serum levels of IL-12 and IL-10 in mice after infected with Babesia microti （B. microti） and Babesia rodhaini （B. rodhaini） were examined. Collected the mice serum and examined the concentration of IL-12 and IL-10 by using ELISA after infection with B. microti and B. rodhaini at 0, 3, 6, 9, 12, 18, 24, 36, 72, 96 h in mice. The results showed that B. microti infection resulted in IL-12 increasing, which peaked at 3 and 24 h after the infection, while same infection did not induce a significant change in IL-10 compared to uninfected mice. When mice were infected with B. rodhaini, any significant changes were not decteted both in IL-12 and IL-10 in comparison with uninfected animals during the period of 3-72 h after infection. Instead, a significant decline in IL-12 and IL-10 was found compared to uninfected mice 96 h after infection with B. rodhaini. It indicates that the mutagenetic cytokine is IL-12 in the serum of mice after infection with B. microti, and no any significant changes were detected in both IL-12 and IL-10 from 0 to 72 h after infected with B. rodhaini.展开更多
Background: Babesiosis is an emerging tick-borne zoonotic infectious disease.Babesia microti is responsible for most cases of human babesiosis globally.It is important to investigate the prevalence of B.microti in the...Background: Babesiosis is an emerging tick-borne zoonotic infectious disease.Babesia microti is responsible for most cases of human babesiosis globally.It is important to investigate the prevalence of B.microti in the mammalian host population of a specific region in order to elucidate mechanisms of pathogen transmission and to define geographic areas where humans face the greatest risk of exposure.The aim of this study is to understand the prevalence and genotypes of B.microti in the small mammals that are found in Beijing,China.Methods:: We trapped small mammals from all of the 16 urban,suburban,and outer suburban districts of Beijing during the years 2014,2017 and 2018.Genomic DNA was extracted from the heart tissues individually and the Babesia 18S rRNA gene was detected by PCR.The genotypes of B.microti were identified based on sequence alignments and phylogenetic analysis.The morphology of the parasites was observed under light microscopy.The risk factors were analyzed statistically based on both univariate analyses and multivariate logistic regression.Results: A total of 1391 small mammals were collected.Positive infection of B.microti was detected in 12.1%(168/1391)of small mammals from 15 out of the 16 districts.Both Kobe-type and U.S.-type B.microti,accounting for 9.5%and 2.7%,respectively,were identified.Classic diverse morphologic forms of B.microti were observed.Specific types of ecological habitats including shrub areas,broad-leaved forest,and cropland were revealed to be risk factors associated with B.microti infection.Conclusions: This study demonstrated the wide prevalence of B.microti infection in eight species of small mammals in Beijing,with Kobe-type more prevalent than U.S.-type.This study provides fundamental information for the development of informed prevention and control measures by public health authorities;the data gathered indicates a need for further monitoring of both clinical diseases in individuals presenting with babesiosis-like symptoms,as well as the infection status of ticks in high risk areas.展开更多
目的:考察布渣叶固形物质量、总黄酮提取物的溶解度,研究温度、湿度、强光照射等对其稳定性的影响。方法:以总黄酮、牡荆苷、异牡荆苷、水仙苷为指标,采用摇瓶法测定布渣叶总黄酮提取物在水、0.1 mol·L-1盐酸溶液和p H 6.8磷酸盐...目的:考察布渣叶固形物质量、总黄酮提取物的溶解度,研究温度、湿度、强光照射等对其稳定性的影响。方法:以总黄酮、牡荆苷、异牡荆苷、水仙苷为指标,采用摇瓶法测定布渣叶总黄酮提取物在水、0.1 mol·L-1盐酸溶液和p H 6.8磷酸盐缓冲液(PBS)中的表观溶解度与平衡溶解度,并考察光照、温度、湿度对其稳定性的影响。结果:布渣叶总黄酮提取物表观溶解度及总黄酮的平衡溶解度在0.1 mol·L-1、盐酸溶液水和p H 6.8 PBS中均属于略溶;以牡荆苷、异牡荆苷为指标,布渣叶总黄酮提取物在0.1 mol·L-1盐酸溶液、水和p H 6.8 PBS中平衡溶解度均为几乎不溶;以水仙苷为指标,布渣叶总黄酮提取物在0.1 mol·L-1盐酸溶液、水和p H 6.8 PBS中平衡溶解度均为极微溶解。稳定性试验结果显示光照、高温、高湿条件对布渣叶总黄酮提取物的含量几乎无影响。结论:随着介质的p H增大,布渣叶总黄酮提取物及其指标成分的溶解度减小。在高湿高温强光照射条件下,布渣叶总黄酮提取物的4个指标成分均较为稳定。研究结果为布渣叶总黄酮提取物的剂型设计以及其他药学研究提供实验依据。展开更多
文摘The serum levels of IL-12 and IL-10 in mice after infected with Babesia microti （B. microti） and Babesia rodhaini （B. rodhaini） were examined. Collected the mice serum and examined the concentration of IL-12 and IL-10 by using ELISA after infection with B. microti and B. rodhaini at 0, 3, 6, 9, 12, 18, 24, 36, 72, 96 h in mice. The results showed that B. microti infection resulted in IL-12 increasing, which peaked at 3 and 24 h after the infection, while same infection did not induce a significant change in IL-10 compared to uninfected mice. When mice were infected with B. rodhaini, any significant changes were not decteted both in IL-12 and IL-10 in comparison with uninfected animals during the period of 3-72 h after infection. Instead, a significant decline in IL-12 and IL-10 was found compared to uninfected mice 96 h after infection with B. rodhaini. It indicates that the mutagenetic cytokine is IL-12 in the serum of mice after infection with B. microti, and no any significant changes were detected in both IL-12 and IL-10 from 0 to 72 h after infected with B. rodhaini.
文摘Background: Babesiosis is an emerging tick-borne zoonotic infectious disease.Babesia microti is responsible for most cases of human babesiosis globally.It is important to investigate the prevalence of B.microti in the mammalian host population of a specific region in order to elucidate mechanisms of pathogen transmission and to define geographic areas where humans face the greatest risk of exposure.The aim of this study is to understand the prevalence and genotypes of B.microti in the small mammals that are found in Beijing,China.Methods:: We trapped small mammals from all of the 16 urban,suburban,and outer suburban districts of Beijing during the years 2014,2017 and 2018.Genomic DNA was extracted from the heart tissues individually and the Babesia 18S rRNA gene was detected by PCR.The genotypes of B.microti were identified based on sequence alignments and phylogenetic analysis.The morphology of the parasites was observed under light microscopy.The risk factors were analyzed statistically based on both univariate analyses and multivariate logistic regression.Results: A total of 1391 small mammals were collected.Positive infection of B.microti was detected in 12.1%(168/1391)of small mammals from 15 out of the 16 districts.Both Kobe-type and U.S.-type B.microti,accounting for 9.5%and 2.7%,respectively,were identified.Classic diverse morphologic forms of B.microti were observed.Specific types of ecological habitats including shrub areas,broad-leaved forest,and cropland were revealed to be risk factors associated with B.microti infection.Conclusions: This study demonstrated the wide prevalence of B.microti infection in eight species of small mammals in Beijing,with Kobe-type more prevalent than U.S.-type.This study provides fundamental information for the development of informed prevention and control measures by public health authorities;the data gathered indicates a need for further monitoring of both clinical diseases in individuals presenting with babesiosis-like symptoms,as well as the infection status of ticks in high risk areas.
文摘目的:考察布渣叶固形物质量、总黄酮提取物的溶解度,研究温度、湿度、强光照射等对其稳定性的影响。方法:以总黄酮、牡荆苷、异牡荆苷、水仙苷为指标,采用摇瓶法测定布渣叶总黄酮提取物在水、0.1 mol·L-1盐酸溶液和p H 6.8磷酸盐缓冲液(PBS)中的表观溶解度与平衡溶解度,并考察光照、温度、湿度对其稳定性的影响。结果:布渣叶总黄酮提取物表观溶解度及总黄酮的平衡溶解度在0.1 mol·L-1、盐酸溶液水和p H 6.8 PBS中均属于略溶;以牡荆苷、异牡荆苷为指标,布渣叶总黄酮提取物在0.1 mol·L-1盐酸溶液、水和p H 6.8 PBS中平衡溶解度均为几乎不溶;以水仙苷为指标,布渣叶总黄酮提取物在0.1 mol·L-1盐酸溶液、水和p H 6.8 PBS中平衡溶解度均为极微溶解。稳定性试验结果显示光照、高温、高湿条件对布渣叶总黄酮提取物的含量几乎无影响。结论:随着介质的p H增大,布渣叶总黄酮提取物及其指标成分的溶解度减小。在高湿高温强光照射条件下,布渣叶总黄酮提取物的4个指标成分均较为稳定。研究结果为布渣叶总黄酮提取物的剂型设计以及其他药学研究提供实验依据。
