The optimal process conditions for solvent-free microwave extraction(SFME)of essential oils from Cinnamomum longepaniculatum deciduous leaves after moisture conditioning were established by response surface methodolog...The optimal process conditions for solvent-free microwave extraction(SFME)of essential oils from Cinnamomum longepaniculatum deciduous leaves after moisture conditioning were established by response surface methodology(RSM).A Box-Behnken design(BBD)was applied to evaluate the effects of three independent variables:moisture content(A:54%–74%),microwave power(B:300–500 W)and microwave time(C:20–40 min),on the extraction yield of essential oil.The compounds of the essential oils obtained by SFME,hydro-distillation and microwaveassisted hydro-distillation(MADE)were identified by gas chromatography-mass spectrometry(GC-MS),and the total lipids of C.longepaniculatum fresh leaves and deciduous leaves were analyzed.The correlation analysis of the response regression model indicated that quadratic polynomial model could be employed to optimize the extraction of essential oil.The optimal extraction condition was A:58%,B:400 W and C:28 min.In the optimal condition,the maximum extraction yield was 4.475 mL/100 g dw and higher than that by MADE.The main compound of the essential oil was eucalyptol(63.54%),and total oxygenated compounds was 78.95%,mainly caused by SFME and the metabolism of endophytic bacteria with decreasing content of phospholipids and fatty acids.Analysis of variance under the extraction condition illustrated high fitness of the model and the success of RSM for optimizing and reflecting the expected process condition.SFME combined with moisture regulation was an effective method for extracting essential oil from C.longepaniculatum deciduous leaves.展开更多
Process parameters of microwave assisted extraction (MAE) of the polysaccharides from pumpkin viz. extraction temperature, time and liquid-solid ratio were studied by using single factor and response surface methodo...Process parameters of microwave assisted extraction (MAE) of the polysaccharides from pumpkin viz. extraction temperature, time and liquid-solid ratio were studied by using single factor and response surface methodology method. The results showed that the liquid-solid ratio was the most important factor in polysaccharides yield, followed the extraction temperature was the least important factor. The optimum microwave assisted extraction co by ndi extraction time, and tions for the highest polysaccharides yield from pumpkin (16.76%-4-0.38%) were obtained by using the response surface methodology with extraction time of 29 min, an extraction temperature of 79 ℃ and a liquid-solid ratio of 22 mL·g^-1. Validation experiment result well agreed with predicted value.展开更多
Orange pomace is the solid waste of the orange juice industry which accounts for approximately 50%of the quantity of the fruits processed into juice and is a good raw material for production of high added value produc...Orange pomace is the solid waste of the orange juice industry which accounts for approximately 50%of the quantity of the fruits processed into juice and is a good raw material for production of high added value products with diverse uses.Orange pomace is rich in polyphenols and flavonoids which can substitute the potentially hazardous or less desirable chemical antioxidants/antimicrobials used in agro-food and cosmetics industry.In this work,an eco-friendly aqueous microwave assisted extraction of orange pomace was investigated and optimized in order to produce aqueous bioactive antioxidant/antimicrobial extracts.A three factorial Response Surface Optimization methodology with centered Box&Behnken experimental design was used to obtain optimum values of total polyphenols and total flavonoids and build predictive models for their optimal extraction conditions.The three optimization factors in terms of applied process parameters were(a)water/solid ratio,(b)extraction temperature and(c)extraction time.The effectiveness and statistical soundness of the two corresponding models regarding optimal total polyphenols and flavonoids were verified by Analysis of Variance(ANOVA).展开更多
Pomegranate pomace is the solid waste of the pomegranate juice industry which accounts for approximately 50%of the quantity of the fruits,which is processed into juice and is a good raw material for production of high...Pomegranate pomace is the solid waste of the pomegranate juice industry which accounts for approximately 50%of the quantity of the fruits,which is processed into juice and is a good raw material for production of high added value products with diverse uses.Pomegranate pomace is rich in polyphenols and flavonoids which could substitute the potentially hazardous synthetic antioxidants/antimicrobials used in agro-food and cosmetics sectors.In this work,eco-friendly aqueous microwave assisted extraction of pomegranate pomace was investigated and optimized in order to produce effectively novel natural antioxidant/antimicrobial extracts.A three-factorial response surface optimization methodology with centered Box&Behnken experimental design was used to obtain the predictive models and the maximum values of total polyphenols,total flavonoids and total antioxidant capacity(TAC).The three optimization factors involved were:(a)water/solid ratio;(b)extraction temperature;(c)extraction time and the effectiveness and robustness of the three models were statistically verified by ANOVA.展开更多
An improved solvent-free microwave extraction(ISFME) was used for the extraction of essential oil from dried Cinnamomum cassia Presl. Two kinds of solid microwave absorption media[carbonyl iron powder(CIP) and gra...An improved solvent-free microwave extraction(ISFME) was used for the extraction of essential oil from dried Cinnamomum cassia Presl. Two kinds of solid microwave absorption media[carbonyl iron powder(CIP) and graphite powder(GP)] were used. When ISFME was applied, the heating rate was enhanced and the extraction time was obviously shorter than that consumed in conventional solvent-free microwave extraction(CSFME). Twenty-eight kinds of compounds in the essential oil were identified, and the total content fractions of the compounds obtained by CIP-ISFME, GP-ISFME, CSFME, and hydrodistillation(HD) were 96.65%, 96.06%, 97.22%, and 96.29%, respectively. The compounds in the essential oil obtained from Cinnamomum cassia Presl. by ISFME, CSFME, and HD were compared and the quantity of the essential oil was almost the same. The ISFME has been proved to be a feasible way to extract essential oil from dried samples, and there are several obvious advantages in ISFME over those of HD and CSFME, for example, shorter extraction time(30 min) and lower energy consumption.展开更多
In this study, laurel essential oils were obtained by using solvent-free microwave extraction (SFME) and hydrodistillation (HD) methods from Laurus nobilis leaves and determined their antioxidant and antimicrobial act...In this study, laurel essential oils were obtained by using solvent-free microwave extraction (SFME) and hydrodistillation (HD) methods from Laurus nobilis leaves and determined their antioxidant and antimicrobial activity. Extraction time was reduced by about 43% in SFME at 622 W and 67% in SFME at 249 W compared to hydrodistillation. Essential oil of laurel was extracted by SFME at 622 W (100%) and 249 W (40%) power levels and HD inhibited oxidation generated by ABTS radical by 93.88%, 94.13% and 92.06%, respectively. Trolox equivalent antioxidant capacities (TEAC) of essential oils were 0.18 mM/mL oil for SFME at 622 W, 1.36 mM/mL oil for SFME at 249 W and 2.40 mM/mL oil for HD (p < 0.05). Essential oils of L. nobilis were extracted by SFME at 100% and 40% power levels and HD inhibited linoleic acid peroxidation by 70.57%, 63.53% and 89.18% respectively. Inhibition effects of laurel essential oils obtained by SFME at different power levels and HD on DPPH radical cation oxidation were not significantly different. The strongest antioxidant activity against DPPH radical was found in the essential oil obtained by SFME at 100% power level. Essential oils displayed antimicrobial activity against Staphylococcus aureus 6538P, Escherichia coli O157:H7 and Salmonella typhimurium NRRL E 4463 except for Listeria monocytogenes. The inhibitory effect on Staphylococcus aureus 6538P survival of laurel oil obtained from SFME by using lower power level was found to be lower than that obtained from SFME at 100% power level and HD展开更多
To analyze the chemical constituents of volatile oils from the fruit of Periploca forrestii Schltr.which were collected in Guizhou province.This volatile oils were extracted by simultaneous distillation extraction (SD...To analyze the chemical constituents of volatile oils from the fruit of Periploca forrestii Schltr.which were collected in Guizhou province.This volatile oils were extracted by simultaneous distillation extraction (SDE)and microwave assistant extraction(MAE) method,and ananlyzed by gas chromatography-mass spectrometry(GC-MS)method.The main chemical compounds were methyl salicylate,β-amyrin and β-amyrin acetate etc.The compounds from the volatile oils extracted with SDE and MAE are quite different.The results provide scientific proof for the further development and utilization of Periploca forrestii Schltr.展开更多
Objective: To study and compare the conventional extraction procedure with microwave assisted extraction (MAE) for some Ayurvedic Rasayana drugs and to evaluate their antioxidant potential and carry out the charact...Objective: To study and compare the conventional extraction procedure with microwave assisted extraction (MAE) for some Ayurvedic Rasayana drugs and to evaluate their antioxidant potential and carry out the characterization of extracts by thin layer chromatography. Methods: Three Ayurvedic rasayana plants Allium sativum Linn., Bombax ceiba Linn. and Inula racemosa Hook. were evaluated for an improved MAE methodology by determining the effects of grinding degree, extraction solvent, effect of dielectric constant and duration of time on the extractive value. Antioxidant potential of all three drugs was evaluated with 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical scavenging activity and reducing power was determined by using Gallic acid as standard. Further thin layer chromatographic (TLC) analysis was performed on pre-activated Silica Gel G plates and Rf value were compared with those reported for the important biomarkers. Results: The total extractive value for Allium sativum Linn. was 36.95% (w/w) and 49.95% (w/w) for ethanol extraction respectively. In case of Bombax ceiba Linn. the yield of aqueous extract by MAE was 50% (w/w) compared to 42% (w/w) in ethanol (50% v/v). Percent yield of Inula racernosa Hook. in aqueous extract was found to be 27.55% (w/w) which was better than ethanol extract (50%) where the yield was 25.95% (w/w). Upon antioxidant activity evaluation A. safivum extract showed an absorbance of 0.980 ± 0.92 at concentration of 500 μg with maximum reducing capacity. This was followed by B. ceiba Linn. 0.825 ± 0.98 and I. racemosa Hook. with 0.799 ± 2.01 at a concentration of 500 μ g. TLC based standardization of A. sativum Linn. extract shows single spot with Rf value of 0.38, B. ceiba Linn. extract shows Rf values were 0.23, 0.58, 0.77, 0.92 and I. racemosa Hook. extract spot had a Rf value of 0.72. Conclusions: A significant improvement in extractive values was observed as a factor of time and other advantages by using MAE technology. All three drugs have high antioxidant potential and a TLC profiling similar to reported ones. The presence of fructan type polysaccharide can be further utilized for bioactivity directed fracUonation and evaluation of immunomodulatory activity.展开更多
In this study the antioxidant activity of barley malt rootlet (BMR) extracts w</span><span style="font-family:"">as</span><span style="font-family:""> evaluated in...In this study the antioxidant activity of barley malt rootlet (BMR) extracts w</span><span style="font-family:"">as</span><span style="font-family:""> evaluated in heat treated corn oil up to 5 hours at 185</span><span style="font-family:"">°</span><span style="font-family:"">C frying temperature. The antioxidant activity </span><span style="font-family:"">of </span><span style="font-family:"">BMR extracts was measured at 25, 50, 100 and 150 ppm concentrations. The free and bound antioxidant phenolics were extracted from BMR using three different extraction methods. Conventional solvent extraction (CSE), microwave assisted extraction (MAE) and autoclave assisted pretreated solvent extraction (APSE). In the present experiment, the total phenolic content and antioxidant activity of the various extracts w</span><span style="font-family:"">ere</span><span style="font-family:""> measured. Thiobarbituric acid reactive substances (TBARS) assay was used to evaluate the ability of the BMR to protect lipid peroxidation in corn oil at 185</span><span style="font-family:"">°</span><span style="font-family:"">C frying temperature. The formation of TBARS at 5 hours of heat treated corn oil ha</span><span style="font-family:"">s</span><span style="font-family:""> shown similar antioxidant levels in 150 ppm butylated hydroxytoluene (BHT) or MAE free phenolic extract added to corn oil. TBARS value for BHT was 1.896 ± 0.013 μg/mL of corn oil and for MAE was 1.896 ± 0.034 μg/mL of corn oil. The highest level of antioxidant activity was found for the free phenolic extracts. The order of inhibition of oxidation was found to be for free phenolics as follows: BHT (100 ppm) > APSE (50 ppm) > MAE (100 ppm) > CSE (100 ppm).展开更多
Recently, biodiesel has received much more attention. Soybean oil, rapeseed oil, palm oil and corn oil are primary feedstock for biodiesel production. However, biodiesel production from these traditional oil-rich crop...Recently, biodiesel has received much more attention. Soybean oil, rapeseed oil, palm oil and corn oil are primary feedstock for biodiesel production. However, biodiesel production from these traditional oil-rich crops is limited by land availability, climate, and environmental and social issues regarding the use of feed and food crops for fuel. Oleaginous microorganisms, including microalgae, bacteria, yeast and fungi can be cultivated with high lipid contents and used as promising feedstock for biodiesel production. However, the high cost of biodiesel production using oil microorganisms has been the biggest obstacle for its industrialization. The process of biodiesel production from microorganisms involves many steps, of which the lipids extraction is the most important and costly. Therefore, searching for an effective and economical extraction system is critical. Various approaches of lipids extraction are discussed in this review, including traditional extraction procedures such as solvent extraction, pressing and solvent integrated extraction, as well as some new procedures.展开更多
基金supports of the Wuhan Scientific and Technical Payoffs Transformation Project(2019030703011505)Enterprise Technology Innovation and Development Projects(2021BLB151)Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2021-OCRI).
文摘The optimal process conditions for solvent-free microwave extraction(SFME)of essential oils from Cinnamomum longepaniculatum deciduous leaves after moisture conditioning were established by response surface methodology(RSM).A Box-Behnken design(BBD)was applied to evaluate the effects of three independent variables:moisture content(A:54%–74%),microwave power(B:300–500 W)and microwave time(C:20–40 min),on the extraction yield of essential oil.The compounds of the essential oils obtained by SFME,hydro-distillation and microwaveassisted hydro-distillation(MADE)were identified by gas chromatography-mass spectrometry(GC-MS),and the total lipids of C.longepaniculatum fresh leaves and deciduous leaves were analyzed.The correlation analysis of the response regression model indicated that quadratic polynomial model could be employed to optimize the extraction of essential oil.The optimal extraction condition was A:58%,B:400 W and C:28 min.In the optimal condition,the maximum extraction yield was 4.475 mL/100 g dw and higher than that by MADE.The main compound of the essential oil was eucalyptol(63.54%),and total oxygenated compounds was 78.95%,mainly caused by SFME and the metabolism of endophytic bacteria with decreasing content of phospholipids and fatty acids.Analysis of variance under the extraction condition illustrated high fitness of the model and the success of RSM for optimizing and reflecting the expected process condition.SFME combined with moisture regulation was an effective method for extracting essential oil from C.longepaniculatum deciduous leaves.
基金Supported by National Natural Science Foundation (31071579)Key Program of Heilongjiang Province Science Foundation (ZP201013)
文摘Process parameters of microwave assisted extraction (MAE) of the polysaccharides from pumpkin viz. extraction temperature, time and liquid-solid ratio were studied by using single factor and response surface methodology method. The results showed that the liquid-solid ratio was the most important factor in polysaccharides yield, followed the extraction temperature was the least important factor. The optimum microwave assisted extraction co by ndi extraction time, and tions for the highest polysaccharides yield from pumpkin (16.76%-4-0.38%) were obtained by using the response surface methodology with extraction time of 29 min, an extraction temperature of 79 ℃ and a liquid-solid ratio of 22 mL·g^-1. Validation experiment result well agreed with predicted value.
基金co-financed by the European Regional Development Fund of the European Union and Greek national funds through the Operational Program Competitiveness,Entrepreneurship and Innovation,under the call Research-Create-Innovate(project code:T1EDK-03942).
文摘Orange pomace is the solid waste of the orange juice industry which accounts for approximately 50%of the quantity of the fruits processed into juice and is a good raw material for production of high added value products with diverse uses.Orange pomace is rich in polyphenols and flavonoids which can substitute the potentially hazardous or less desirable chemical antioxidants/antimicrobials used in agro-food and cosmetics industry.In this work,an eco-friendly aqueous microwave assisted extraction of orange pomace was investigated and optimized in order to produce aqueous bioactive antioxidant/antimicrobial extracts.A three factorial Response Surface Optimization methodology with centered Box&Behnken experimental design was used to obtain optimum values of total polyphenols and total flavonoids and build predictive models for their optimal extraction conditions.The three optimization factors in terms of applied process parameters were(a)water/solid ratio,(b)extraction temperature and(c)extraction time.The effectiveness and statistical soundness of the two corresponding models regarding optimal total polyphenols and flavonoids were verified by Analysis of Variance(ANOVA).
文摘Pomegranate pomace is the solid waste of the pomegranate juice industry which accounts for approximately 50%of the quantity of the fruits,which is processed into juice and is a good raw material for production of high added value products with diverse uses.Pomegranate pomace is rich in polyphenols and flavonoids which could substitute the potentially hazardous synthetic antioxidants/antimicrobials used in agro-food and cosmetics sectors.In this work,eco-friendly aqueous microwave assisted extraction of pomegranate pomace was investigated and optimized in order to produce effectively novel natural antioxidant/antimicrobial extracts.A three-factorial response surface optimization methodology with centered Box&Behnken experimental design was used to obtain the predictive models and the maximum values of total polyphenols,total flavonoids and total antioxidant capacity(TAC).The three optimization factors involved were:(a)water/solid ratio;(b)extraction temperature;(c)extraction time and the effectiveness and robustness of the three models were statistically verified by ANOVA.
文摘An improved solvent-free microwave extraction(ISFME) was used for the extraction of essential oil from dried Cinnamomum cassia Presl. Two kinds of solid microwave absorption media[carbonyl iron powder(CIP) and graphite powder(GP)] were used. When ISFME was applied, the heating rate was enhanced and the extraction time was obviously shorter than that consumed in conventional solvent-free microwave extraction(CSFME). Twenty-eight kinds of compounds in the essential oil were identified, and the total content fractions of the compounds obtained by CIP-ISFME, GP-ISFME, CSFME, and hydrodistillation(HD) were 96.65%, 96.06%, 97.22%, and 96.29%, respectively. The compounds in the essential oil obtained from Cinnamomum cassia Presl. by ISFME, CSFME, and HD were compared and the quantity of the essential oil was almost the same. The ISFME has been proved to be a feasible way to extract essential oil from dried samples, and there are several obvious advantages in ISFME over those of HD and CSFME, for example, shorter extraction time(30 min) and lower energy consumption.
基金The TUBİTAK,The Scientific and Technologic Research Council of Turkey(Grant No.TOVAG 104 O 265)financially supported this study.
文摘In this study, laurel essential oils were obtained by using solvent-free microwave extraction (SFME) and hydrodistillation (HD) methods from Laurus nobilis leaves and determined their antioxidant and antimicrobial activity. Extraction time was reduced by about 43% in SFME at 622 W and 67% in SFME at 249 W compared to hydrodistillation. Essential oil of laurel was extracted by SFME at 622 W (100%) and 249 W (40%) power levels and HD inhibited oxidation generated by ABTS radical by 93.88%, 94.13% and 92.06%, respectively. Trolox equivalent antioxidant capacities (TEAC) of essential oils were 0.18 mM/mL oil for SFME at 622 W, 1.36 mM/mL oil for SFME at 249 W and 2.40 mM/mL oil for HD (p < 0.05). Essential oils of L. nobilis were extracted by SFME at 100% and 40% power levels and HD inhibited linoleic acid peroxidation by 70.57%, 63.53% and 89.18% respectively. Inhibition effects of laurel essential oils obtained by SFME at different power levels and HD on DPPH radical cation oxidation were not significantly different. The strongest antioxidant activity against DPPH radical was found in the essential oil obtained by SFME at 100% power level. Essential oils displayed antimicrobial activity against Staphylococcus aureus 6538P, Escherichia coli O157:H7 and Salmonella typhimurium NRRL E 4463 except for Listeria monocytogenes. The inhibitory effect on Staphylococcus aureus 6538P survival of laurel oil obtained from SFME by using lower power level was found to be lower than that obtained from SFME at 100% power level and HD
文摘To analyze the chemical constituents of volatile oils from the fruit of Periploca forrestii Schltr.which were collected in Guizhou province.This volatile oils were extracted by simultaneous distillation extraction (SDE)and microwave assistant extraction(MAE) method,and ananlyzed by gas chromatography-mass spectrometry(GC-MS)method.The main chemical compounds were methyl salicylate,β-amyrin and β-amyrin acetate etc.The compounds from the volatile oils extracted with SDE and MAE are quite different.The results provide scientific proof for the further development and utilization of Periploca forrestii Schltr.
基金the Advance Institute of Biotech and Paramedical Sciences for providing all required facilities regarding the fulfillment of this research project
文摘Objective: To study and compare the conventional extraction procedure with microwave assisted extraction (MAE) for some Ayurvedic Rasayana drugs and to evaluate their antioxidant potential and carry out the characterization of extracts by thin layer chromatography. Methods: Three Ayurvedic rasayana plants Allium sativum Linn., Bombax ceiba Linn. and Inula racemosa Hook. were evaluated for an improved MAE methodology by determining the effects of grinding degree, extraction solvent, effect of dielectric constant and duration of time on the extractive value. Antioxidant potential of all three drugs was evaluated with 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical scavenging activity and reducing power was determined by using Gallic acid as standard. Further thin layer chromatographic (TLC) analysis was performed on pre-activated Silica Gel G plates and Rf value were compared with those reported for the important biomarkers. Results: The total extractive value for Allium sativum Linn. was 36.95% (w/w) and 49.95% (w/w) for ethanol extraction respectively. In case of Bombax ceiba Linn. the yield of aqueous extract by MAE was 50% (w/w) compared to 42% (w/w) in ethanol (50% v/v). Percent yield of Inula racernosa Hook. in aqueous extract was found to be 27.55% (w/w) which was better than ethanol extract (50%) where the yield was 25.95% (w/w). Upon antioxidant activity evaluation A. safivum extract showed an absorbance of 0.980 ± 0.92 at concentration of 500 μg with maximum reducing capacity. This was followed by B. ceiba Linn. 0.825 ± 0.98 and I. racemosa Hook. with 0.799 ± 2.01 at a concentration of 500 μ g. TLC based standardization of A. sativum Linn. extract shows single spot with Rf value of 0.38, B. ceiba Linn. extract shows Rf values were 0.23, 0.58, 0.77, 0.92 and I. racemosa Hook. extract spot had a Rf value of 0.72. Conclusions: A significant improvement in extractive values was observed as a factor of time and other advantages by using MAE technology. All three drugs have high antioxidant potential and a TLC profiling similar to reported ones. The presence of fructan type polysaccharide can be further utilized for bioactivity directed fracUonation and evaluation of immunomodulatory activity.
文摘In this study the antioxidant activity of barley malt rootlet (BMR) extracts w</span><span style="font-family:"">as</span><span style="font-family:""> evaluated in heat treated corn oil up to 5 hours at 185</span><span style="font-family:"">°</span><span style="font-family:"">C frying temperature. The antioxidant activity </span><span style="font-family:"">of </span><span style="font-family:"">BMR extracts was measured at 25, 50, 100 and 150 ppm concentrations. The free and bound antioxidant phenolics were extracted from BMR using three different extraction methods. Conventional solvent extraction (CSE), microwave assisted extraction (MAE) and autoclave assisted pretreated solvent extraction (APSE). In the present experiment, the total phenolic content and antioxidant activity of the various extracts w</span><span style="font-family:"">ere</span><span style="font-family:""> measured. Thiobarbituric acid reactive substances (TBARS) assay was used to evaluate the ability of the BMR to protect lipid peroxidation in corn oil at 185</span><span style="font-family:"">°</span><span style="font-family:"">C frying temperature. The formation of TBARS at 5 hours of heat treated corn oil ha</span><span style="font-family:"">s</span><span style="font-family:""> shown similar antioxidant levels in 150 ppm butylated hydroxytoluene (BHT) or MAE free phenolic extract added to corn oil. TBARS value for BHT was 1.896 ± 0.013 μg/mL of corn oil and for MAE was 1.896 ± 0.034 μg/mL of corn oil. The highest level of antioxidant activity was found for the free phenolic extracts. The order of inhibition of oxidation was found to be for free phenolics as follows: BHT (100 ppm) > APSE (50 ppm) > MAE (100 ppm) > CSE (100 ppm).
基金Acknowledgements This work was financed by the National Natural Science Foundation of China (Grant No. 20976140). The authors are grateful to Department of Chemical Engineering, School of Engineering, University of Louisiana at Lafayette, USA and Key Laboratory for Green Chemical Process of Ministry of Education for providing the condition of researching.
文摘Recently, biodiesel has received much more attention. Soybean oil, rapeseed oil, palm oil and corn oil are primary feedstock for biodiesel production. However, biodiesel production from these traditional oil-rich crops is limited by land availability, climate, and environmental and social issues regarding the use of feed and food crops for fuel. Oleaginous microorganisms, including microalgae, bacteria, yeast and fungi can be cultivated with high lipid contents and used as promising feedstock for biodiesel production. However, the high cost of biodiesel production using oil microorganisms has been the biggest obstacle for its industrialization. The process of biodiesel production from microorganisms involves many steps, of which the lipids extraction is the most important and costly. Therefore, searching for an effective and economical extraction system is critical. Various approaches of lipids extraction are discussed in this review, including traditional extraction procedures such as solvent extraction, pressing and solvent integrated extraction, as well as some new procedures.