In western China, most petroliferous basins are superposed due to their multi-periodic tectonic evolution, and the mechanisms of petroleum migration and accumulation are so complex that much more sophis- ticated metho...In western China, most petroliferous basins are superposed due to their multi-periodic tectonic evolution, and the mechanisms of petroleum migration and accumulation are so complex that much more sophis- ticated methodologies are necessary for depiction of these mechanisms and identification of petroleum occurrences. For this purpose, in this article, a new methodology was formulated which includes： （I） ver- tical identification of petroleum migration and accumulation fluid dynamic systems in the superposed basins; （2） analysis of the effect of large scale regional faults and fault combinations on the fluids exchange between the vertically identified different systems; （3） analysis of petroleum migration and accumulation in each vertically identified system, and establishment of appropriate geological model of petroleum migration and accumulation for each vertically identified system. Using this methodology, the satisfactory results obtained in the Lunnan Uplift of Tarim Basin and Ludong Uplift of Jungar Basin case studies are： （1） existence of different vertical fluid dynamic systems in western China＇s superposed basins which are very necessary for understanding the mechanism of petroleum migration and accumu- lation; （2） in deep system, long-distance lateral petroleum migration and accumulation mainly take place along the long time exposed unconformity with weathered, fractured or karst reservoir rocks; （3） regio- nal faults are the main conducts for fluids migration from deep system up to middle and/or upper sys- tems. As to middle and/or upper systems, regional faults play a role of ＂petroleum source＂. Small faults within middle and/or upper systems conduct petroleum to carrier beds with less impeding force; （4） petroleum migrated from deep system vertically up to middle and/or upper systems will migrate lat- erally in carrier beds of these systems and accumulate to form nools near or far from faults.展开更多
目的探讨MAP4K1在黑色素瘤(cutaneous melanoma,CM)细胞和组织中表达及对恶性CM细胞A375迁移、侵袭的影响和机制。方法收集自2010年1月至2015年12月重庆市中医院皮肤科诊治的CM患者54例,采用GEPIA在线分析CM组织中MAP4K1的表达,实时定...目的探讨MAP4K1在黑色素瘤(cutaneous melanoma,CM)细胞和组织中表达及对恶性CM细胞A375迁移、侵袭的影响和机制。方法收集自2010年1月至2015年12月重庆市中医院皮肤科诊治的CM患者54例,采用GEPIA在线分析CM组织中MAP4K1的表达,实时定量聚合酶链法和免疫组织化学法检测CM组织中MAP4K1 m RNA和蛋白的表达水平;以MAP4K1基因表达最高的A375细胞为研究对象,采用脂质体转染法将MAP4K1抑制质粒和对照质粒转染至A375细胞;免疫蛋白印迹实验和实时定量聚合酶链法分别检测MAP4K1的基因敲除;分别通过Transwell实验检测MAP4K1敲除后对细胞侵袭的影响;荧光素酶报告实验检测MAP4K1对YAP/TAZ的调控;GEPIA验证MAP4K1与Hippo-YAP通路下游靶点的表达相关性;免疫蛋白印迹实验验证MAP4K1敲除后对Hippo-YAP信号通路的影响。结果与癌旁组织相比,MAP4K1 m RNA和蛋白在皮肤恶性CM细胞和组织中的表达上调(P<0.001);MAP4K1基因敲除A375细胞迁移和侵袭的能力明显受到抑制(P<0.001);MAP4K1敲除后YAP/TAZ的转录活性及下游的靶分子下调;MAP4K1基因敲除能抑制Hippo-YAP通路传导。结论MAP4K1在CM中表达上调,可能作为潜在的癌基因调控Hippo-YAP通路促进CM的发生发展。展开更多
基金provided by the National Basic Research Program of China (No. 2006CB20235)
文摘In western China, most petroliferous basins are superposed due to their multi-periodic tectonic evolution, and the mechanisms of petroleum migration and accumulation are so complex that much more sophis- ticated methodologies are necessary for depiction of these mechanisms and identification of petroleum occurrences. For this purpose, in this article, a new methodology was formulated which includes： （I） ver- tical identification of petroleum migration and accumulation fluid dynamic systems in the superposed basins; （2） analysis of the effect of large scale regional faults and fault combinations on the fluids exchange between the vertically identified different systems; （3） analysis of petroleum migration and accumulation in each vertically identified system, and establishment of appropriate geological model of petroleum migration and accumulation for each vertically identified system. Using this methodology, the satisfactory results obtained in the Lunnan Uplift of Tarim Basin and Ludong Uplift of Jungar Basin case studies are： （1） existence of different vertical fluid dynamic systems in western China＇s superposed basins which are very necessary for understanding the mechanism of petroleum migration and accumu- lation; （2） in deep system, long-distance lateral petroleum migration and accumulation mainly take place along the long time exposed unconformity with weathered, fractured or karst reservoir rocks; （3） regio- nal faults are the main conducts for fluids migration from deep system up to middle and/or upper sys- tems. As to middle and/or upper systems, regional faults play a role of ＂petroleum source＂. Small faults within middle and/or upper systems conduct petroleum to carrier beds with less impeding force; （4） petroleum migrated from deep system vertically up to middle and/or upper systems will migrate lat- erally in carrier beds of these systems and accumulate to form nools near or far from faults.
文摘目的探讨MAP4K1在黑色素瘤(cutaneous melanoma,CM)细胞和组织中表达及对恶性CM细胞A375迁移、侵袭的影响和机制。方法收集自2010年1月至2015年12月重庆市中医院皮肤科诊治的CM患者54例,采用GEPIA在线分析CM组织中MAP4K1的表达,实时定量聚合酶链法和免疫组织化学法检测CM组织中MAP4K1 m RNA和蛋白的表达水平;以MAP4K1基因表达最高的A375细胞为研究对象,采用脂质体转染法将MAP4K1抑制质粒和对照质粒转染至A375细胞;免疫蛋白印迹实验和实时定量聚合酶链法分别检测MAP4K1的基因敲除;分别通过Transwell实验检测MAP4K1敲除后对细胞侵袭的影响;荧光素酶报告实验检测MAP4K1对YAP/TAZ的调控;GEPIA验证MAP4K1与Hippo-YAP通路下游靶点的表达相关性;免疫蛋白印迹实验验证MAP4K1敲除后对Hippo-YAP信号通路的影响。结果与癌旁组织相比,MAP4K1 m RNA和蛋白在皮肤恶性CM细胞和组织中的表达上调(P<0.001);MAP4K1基因敲除A375细胞迁移和侵袭的能力明显受到抑制(P<0.001);MAP4K1敲除后YAP/TAZ的转录活性及下游的靶分子下调;MAP4K1基因敲除能抑制Hippo-YAP通路传导。结论MAP4K1在CM中表达上调,可能作为潜在的癌基因调控Hippo-YAP通路促进CM的发生发展。
