The effects of dietary supplementation with Clostridium butyricum on growth performance and humoral immune response in Miichthys miiuy were evaluated. One hundred and fifty Miichthys miiuy weighing approximately 200-2...The effects of dietary supplementation with Clostridium butyricum on growth performance and humoral immune response in Miichthys miiuy were evaluated. One hundred and fifty Miichthys miiuy weighing approximately 200-260 g were divided into five groups and reared in 15 tanks with closed circuiting culture system. The animals were fed 5 diets： basal diet only （control） or supplemented of the basal diet with C. butyricum at doses of 10^3 （CB1）, 10^5 （CB2）, 10^7 （CB3） or 10^9 （CB4） CFU/g. Compared with the control, the serum phenoloxidase activity was significantly increased by the supplementation （P〈0.05）, acid phosphatases activity was increased significantly （P〈0.05） at the doses of 10^9 CFU/g. Serum lysozyme activity peaked at dose of 10^7 CFU/g and in the skin mucus at dose of 10^9 CFU/g. Immunoglobulin M level in the serum and skin mucus was increased except at dose of 10^3 CFU/g （P〈0.05）. The growth at the dose of 10^9 CFU/g was higher than that of the control （P〈0.05）. It is concluded that supplementation of C. butyricum can mediate the humoral immune responses and improve the growth performance in Miichthys miiuy.展开更多
Effects of dietary supplementation with fructooligosaccharides on the excretion of nitrogen and phosphorus in Miichthys miiuy fries were investigated. Nine hundred Miichthys miiuy fries were divided into 3 groups, eac...Effects of dietary supplementation with fructooligosaccharides on the excretion of nitrogen and phosphorus in Miichthys miiuy fries were investigated. Nine hundred Miichthys miiuy fries were divided into 3 groups, each with triplicates. The basal diet and the basal diet supplemented with carnitine groups were considered as the negative and positive controls respectively. Results showed that the nitrogen concentration in excreted feces decreased significantly in fries fed the diet supplementation with 1000×10?6 fructooligosaccharides and 200×10?6 carnitine (P<0.05). The ammonic-nitrogen concentration decreased significantly in the carnitine group only (P<0.05), indicating the decreasing tendency caused by the supplementation with fructooligosaccha-rides. Supplementation with both did not have significant effects on the concentration of phosphorus in feces of Miichthys miiuy fries.展开更多
Miiuy croaker,Miichthys miiuy is an ecologically important teleost species which is widely distributed in southeast coast of China.In this study,we present a chromosomal-scale genome assembly of the miiuy croaker whic...Miiuy croaker,Miichthys miiuy is an ecologically important teleost species which is widely distributed in southeast coast of China.In this study,we present a chromosomal-scale genome assembly of the miiuy croaker which is an important Sciaenidae fish and economical species.We adopted Oxford Nanopore and Hi-C sequencing techniques to achieve an assembly with high accuracy and completeness.The investigation of genome characteristic and functional features may provide insights into the study of phylogenetic diversity of miiuy croaker.This study can also be beneficial to improve molecular assisted breeding techniques.Moreover,it can be a great resource to better conduct further study of other sciaenids.展开更多
Histamine is a type of biogenic amine,which plays a major role in the health problems associated with seafood consumption.Legislative limits of histamine level in seafood have been set in many countries.This study foc...Histamine is a type of biogenic amine,which plays a major role in the health problems associated with seafood consumption.Legislative limits of histamine level in seafood have been set in many countries.This study focuses on investigating the feasibility and potentiality of determining histamine concentration in fish(Miichthys miiuy)by surface-enhanced Raman spectroscopy(SERS)combined with density functional theory(DFT).Both a gold colloid and a silver colloid were used to determine the enhancement effect for SERS detection of histamine standard solution,and the gold colloid exhibited more effective as compared to the silver colloid.The protocol on extraction of histamine with 12%trichloroacetic acid and adjustment of pH with NaOH solution(5 mol/L)could significantly shorten sample preparation(20 min)and provide clear SERS spectra of histamine.The peaks of histamine molecules were classified using the DFT and five spectra(953 cm^(-1),992 cm^(-1),1106 cm^(-1),1262 cm^(-1)and 1317 cm^(-1))were selected as the characteristic bands of histamine discrimination.Moreover,the intensity of the peak at 1262 cm^(-1)had a good linear relationship with histamine concentration at 5-400 mg/kg with R^(2)=0.9755.It is concluded that the SERS-DFT approach will be a potential method for rapidly and reliably detecting histamine at levels from 5 mg/kg to 400 mg/kg in fresh fish.展开更多
Inflammation is a self-protection mechanism that can be triggered when innate immune cells detect infection.Eradication of pathogen infection requires appropriate immune and inflammatory responses,but excessive inflam...Inflammation is a self-protection mechanism that can be triggered when innate immune cells detect infection.Eradication of pathogen infection requires appropriate immune and inflammatory responses,but excessive inflammatory responses can cause uncontrolled inflammation,autoimmune diseases,or pathogen dissemination.Mounting evidence has shown that microRNAs(miRNAs)in mammals act as important and versatile regulators of innate immunity and inflammation.However,miRNAmediated regulation networks are largely unknown in inflammatory responses in lower vertebrates.Here,miR-144 and miR-217 are identified as negative regulators in teleost inflammatory responses.We find that Vibrio harveyi and lipopolysaccharide(LPS)treatment significantly upregulate the expression of fish miR-144 and miR-217.Upregulated miR-144 and miR-217 suppress LPS-induced inflammatory cytokine expression by targeting nucleotide-binding oligomerization domain-containing protein 1(NOD1),thereby avoiding excessive inflammatory responses.In addition,miR-144 and miR-217 regulate inflammatory responses through NOD1-induced nuclear factor kappa(NF-κB)signaling pathways.These findings demonstrate that miR-144 and miR-217 play regulatory roles in inflammatory responses by modulating the NOD1-induced NF-κB signaling pathway.展开更多
基金Project supported by the Bureau of Science and Technology of Zhejiang Province (No. 2004201), China and the Youth Fund of Ningbo City (No. 2004A620008), China
文摘The effects of dietary supplementation with Clostridium butyricum on growth performance and humoral immune response in Miichthys miiuy were evaluated. One hundred and fifty Miichthys miiuy weighing approximately 200-260 g were divided into five groups and reared in 15 tanks with closed circuiting culture system. The animals were fed 5 diets： basal diet only （control） or supplemented of the basal diet with C. butyricum at doses of 10^3 （CB1）, 10^5 （CB2）, 10^7 （CB3） or 10^9 （CB4） CFU/g. Compared with the control, the serum phenoloxidase activity was significantly increased by the supplementation （P〈0.05）, acid phosphatases activity was increased significantly （P〈0.05） at the doses of 10^9 CFU/g. Serum lysozyme activity peaked at dose of 10^7 CFU/g and in the skin mucus at dose of 10^9 CFU/g. Immunoglobulin M level in the serum and skin mucus was increased except at dose of 10^3 CFU/g （P〈0.05）. The growth at the dose of 10^9 CFU/g was higher than that of the control （P〈0.05）. It is concluded that supplementation of C. butyricum can mediate the humoral immune responses and improve the growth performance in Miichthys miiuy.
基金Project supported by Public Bidding Item in Key Research of ZhejiangProvince (No. 02110281-2)Ningbo Tackle Key Problem forAgricultural Development (No. 2004C-100030), China
文摘Effects of dietary supplementation with fructooligosaccharides on the excretion of nitrogen and phosphorus in Miichthys miiuy fries were investigated. Nine hundred Miichthys miiuy fries were divided into 3 groups, each with triplicates. The basal diet and the basal diet supplemented with carnitine groups were considered as the negative and positive controls respectively. Results showed that the nitrogen concentration in excreted feces decreased significantly in fries fed the diet supplementation with 1000×10?6 fructooligosaccharides and 200×10?6 carnitine (P<0.05). The ammonic-nitrogen concentration decreased significantly in the carnitine group only (P<0.05), indicating the decreasing tendency caused by the supplementation with fructooligosaccha-rides. Supplementation with both did not have significant effects on the concentration of phosphorus in feces of Miichthys miiuy fries.
基金supported by the National Key Research and Development Project(2018YFD0900301)the National Natural Science Foundation of China(31802325).
文摘Miiuy croaker,Miichthys miiuy is an ecologically important teleost species which is widely distributed in southeast coast of China.In this study,we present a chromosomal-scale genome assembly of the miiuy croaker which is an important Sciaenidae fish and economical species.We adopted Oxford Nanopore and Hi-C sequencing techniques to achieve an assembly with high accuracy and completeness.The investigation of genome characteristic and functional features may provide insights into the study of phylogenetic diversity of miiuy croaker.This study can also be beneficial to improve molecular assisted breeding techniques.Moreover,it can be a great resource to better conduct further study of other sciaenids.
基金supported by“863”National High-Tech Research and Development Plan(Project No:2013AA102301)Zhejiang Provincial Science&Technology Innovation team Project.
文摘Histamine is a type of biogenic amine,which plays a major role in the health problems associated with seafood consumption.Legislative limits of histamine level in seafood have been set in many countries.This study focuses on investigating the feasibility and potentiality of determining histamine concentration in fish(Miichthys miiuy)by surface-enhanced Raman spectroscopy(SERS)combined with density functional theory(DFT).Both a gold colloid and a silver colloid were used to determine the enhancement effect for SERS detection of histamine standard solution,and the gold colloid exhibited more effective as compared to the silver colloid.The protocol on extraction of histamine with 12%trichloroacetic acid and adjustment of pH with NaOH solution(5 mol/L)could significantly shorten sample preparation(20 min)and provide clear SERS spectra of histamine.The peaks of histamine molecules were classified using the DFT and five spectra(953 cm^(-1),992 cm^(-1),1106 cm^(-1),1262 cm^(-1)and 1317 cm^(-1))were selected as the characteristic bands of histamine discrimination.Moreover,the intensity of the peak at 1262 cm^(-1)had a good linear relationship with histamine concentration at 5-400 mg/kg with R^(2)=0.9755.It is concluded that the SERS-DFT approach will be a potential method for rapidly and reliably detecting histamine at levels from 5 mg/kg to 400 mg/kg in fresh fish.
基金the National Natural Science Foundation of China(31822057)the National Key Research and Development Project(2018YFD0900503)。
文摘Inflammation is a self-protection mechanism that can be triggered when innate immune cells detect infection.Eradication of pathogen infection requires appropriate immune and inflammatory responses,but excessive inflammatory responses can cause uncontrolled inflammation,autoimmune diseases,or pathogen dissemination.Mounting evidence has shown that microRNAs(miRNAs)in mammals act as important and versatile regulators of innate immunity and inflammation.However,miRNAmediated regulation networks are largely unknown in inflammatory responses in lower vertebrates.Here,miR-144 and miR-217 are identified as negative regulators in teleost inflammatory responses.We find that Vibrio harveyi and lipopolysaccharide(LPS)treatment significantly upregulate the expression of fish miR-144 and miR-217.Upregulated miR-144 and miR-217 suppress LPS-induced inflammatory cytokine expression by targeting nucleotide-binding oligomerization domain-containing protein 1(NOD1),thereby avoiding excessive inflammatory responses.In addition,miR-144 and miR-217 regulate inflammatory responses through NOD1-induced nuclear factor kappa(NF-κB)signaling pathways.These findings demonstrate that miR-144 and miR-217 play regulatory roles in inflammatory responses by modulating the NOD1-induced NF-κB signaling pathway.
