In order to investigate the differences between agronomic traits of dwarf mutant and original material 260 and whether the cause of dwarf is related to GA synthesis or signaling pathway,this experiment used dwarf muta...In order to investigate the differences between agronomic traits of dwarf mutant and original material 260 and whether the cause of dwarf is related to GA synthesis or signaling pathway,this experiment used dwarf mutant 778 and its original material 260 as experimental materials.Morphological observation and determination were performed for agronomic traits on plant height,ear length,internode length,internode number,seed length,seed width and number of seeds in different growth periods and different concentrations.The plants were treated by GA spraying,and the changes of plant height,root length,stem width,leaf length and leaf width were measured.The results are as follows:①The plant height of the dwarf mutant material was significantly different from that of the original high material,which was mainly caused by the difference between above-ground basal part and the length of the first and second elongation joints.②Comparing and analyzing the differences of traits between dwarf mutant material 778 and original high material 260,it was found that the plant height,ear length,internode number,grain number per ear and internode length of dwarf mutant 778 were significantly lower than that of high stalk 260(P<0.01),and the seed length of dwarf mutant 778 was significantly higher than that of high stalk 260(P<0.05).③Different concentrations of gibberellin(0,50,100,200 mg/L)had no significant effect on plant height and root length of dwarf mutant 778(P>0.05).Different concentrations of gibberellin had significant effects on plant height,root length and sensitivity coefficient of high stalk 260(P<0.05).And compared with the control group,all high materials 260 treated with different concentrations of gibberellin performed differently in plant morphology and growth potential.④Under the conditions of 100 and 200 mg/L GA,the difference of plant height between the dwarf mutant and the high stalk control decreased with time,and there was no difference at the end.There were no differences in sensitivity coefficient GRI between different concentrations of gibberellin treatment groups,indicating that the external gibberellin could restore the scorpion dwarf mutant to the original high stalk,and the gene that causes the mutation might be related with the gibberellin synthesis pathway.展开更多
The genetic diversity of 118 accessions of broomcom millet （Panicum miliaceum L.）, collected from various ecological areas, was analyzed. Using 46 SSR （Simple Sequence Repeat） polymorphic markers from rice, wheat,...The genetic diversity of 118 accessions of broomcom millet （Panicum miliaceum L.）, collected from various ecological areas, was analyzed. Using 46 SSR （Simple Sequence Repeat） polymorphic markers from rice, wheat, oat and barley, a total of 226 alleles were found, which exhibited moderate level of diversity. The number of alleles per primer ranged from two to nine, with an average of 4.91. The range of polymorphism information content （PIC） was 0.2844）.980 （average, 0.793）. The expected heterozygosity （He） varied from 0.346 to 0.989, with an average of 0.834. The average coefficient of the genetic similarity of SSR markers among the 118 accessions was 0.609, and it ranged from 0.461 to 0.851. The UPGMA （Unweight Pair Group Method with Arithmetic Mean） clustering analysis at the genetic similarity value of 0.609 grouped the 118 accessions into five groups. Mantel test meant that geographical origin and genetic distance presented positive correlation. The clustering results were consistent with known information on ecological growing areas. The genetic similarity coefficient of the accessions in the Loess Plateau ecotype was significantly lower than those in the other ecotypes. It indicates that the highest level of genetic diversity occurred in the Loess Plateau, which is probably the original site of Panicum miliaceum.展开更多
[目的]以6份不同地理来源的糜子资源为试验材料,基于前期转录组测序获得的1000对SSR引物,选出200对进行多态性检测,以期构建一批可以准确评估糜子种质遗传差异的分子标记.[方法]用Primer Premier 5.0软件设计引物,改良CTAB法提取DNA,PC...[目的]以6份不同地理来源的糜子资源为试验材料,基于前期转录组测序获得的1000对SSR引物,选出200对进行多态性检测,以期构建一批可以准确评估糜子种质遗传差异的分子标记.[方法]用Primer Premier 5.0软件设计引物,改良CTAB法提取DNA,PCR扩增DNA和聚丙烯酰胺凝胶电泳筛选引物多态性;用PowerMarker 3.25和PopGen 1.32计算遗传多样性参数.[结果]200对引物中97对呈单态性,80对呈多态性.单碱基序列重复引物有20对,10对具多态性,其重复基元是A(50%)和T(50%).二碱基序列重复引物有36对,15对具多态性,其碱基重复类型有7种(AG最多,TC、GC和GA次之,CA、TA和AC最少).三碱基序列重复引物有144对,55对具多态性,其碱基重复类型有24种(GGC、GCG和GCC最多,GAA、GCT和CGC等次之,ACC、AGG、CAG、CGT、AAG、AAC、TCG、CGA、ATT、CAA和CCA最少).就引物分辨率(Rp)值而言,1-3碱基序列重复引物分别为0.67-4.67(平均2.07)、1.33-4.33(平均2.73)和0.67-4.00(平均1.83).基于Rp值分析SSR分布频次,发现80个标记分布在5个区间:0-1、1-2、2-3、3-4和4-5,分别包含17(21.25%)、36(45.00%)、11(13.75%)、14(17.50%)和2个(2.50%).就Rp值而言,1-3碱基序列重复引物分别为0.33-0.67(平均0.51)、0.40-0.78(平均0.59)和0.33-0.83(平均0.59).单碱基序列重复标记共检测到22个等位变异,每个位点为2-3个(平均2.2000),其中8个和2个位点分别具2和3个变异;二碱基序列重复标记共检测到38个等位变异,每个位点为2-3个(平均2.5333),其中7个和8个位点分别具2和3个变异;三碱基重复标记共检测到136个等位变异,每个位点为2-3个(平均2.4727),其中29个和26个位点分别具2和3个变异.就多态性信息含量而言,1-3碱基序列重复引物分别为0.3750-0.5355(平均0.4293)、0.2392-0.7438(平均0.4293)和0.2392-0.7438(平均0.3989).就多样性指数而言,1-3碱基序列重复分别为0.6365-1.0776(平均0.7497)、0.5623-1.0986(平均0.8339)和0.5623-1.0889(平均0.8312).[结论]基于转录组测序结果,检测200对SSR引物的多态性,发现177对(88.5%)可以扩增出完整条带,其中80对具多态性,多态率为40%.展开更多
基金Supported by Project of Hebei Province Department of Science and Technology(17396310D).
文摘In order to investigate the differences between agronomic traits of dwarf mutant and original material 260 and whether the cause of dwarf is related to GA synthesis or signaling pathway,this experiment used dwarf mutant 778 and its original material 260 as experimental materials.Morphological observation and determination were performed for agronomic traits on plant height,ear length,internode length,internode number,seed length,seed width and number of seeds in different growth periods and different concentrations.The plants were treated by GA spraying,and the changes of plant height,root length,stem width,leaf length and leaf width were measured.The results are as follows:①The plant height of the dwarf mutant material was significantly different from that of the original high material,which was mainly caused by the difference between above-ground basal part and the length of the first and second elongation joints.②Comparing and analyzing the differences of traits between dwarf mutant material 778 and original high material 260,it was found that the plant height,ear length,internode number,grain number per ear and internode length of dwarf mutant 778 were significantly lower than that of high stalk 260(P<0.01),and the seed length of dwarf mutant 778 was significantly higher than that of high stalk 260(P<0.05).③Different concentrations of gibberellin(0,50,100,200 mg/L)had no significant effect on plant height and root length of dwarf mutant 778(P>0.05).Different concentrations of gibberellin had significant effects on plant height,root length and sensitivity coefficient of high stalk 260(P<0.05).And compared with the control group,all high materials 260 treated with different concentrations of gibberellin performed differently in plant morphology and growth potential.④Under the conditions of 100 and 200 mg/L GA,the difference of plant height between the dwarf mutant and the high stalk control decreased with time,and there was no difference at the end.There were no differences in sensitivity coefficient GRI between different concentrations of gibberellin treatment groups,indicating that the external gibberellin could restore the scorpion dwarf mutant to the original high stalk,and the gene that causes the mutation might be related with the gibberellin synthesis pathway.
基金supported by the Ministry of Science and Technology in China (No.2006BAD02B07-01)
文摘The genetic diversity of 118 accessions of broomcom millet （Panicum miliaceum L.）, collected from various ecological areas, was analyzed. Using 46 SSR （Simple Sequence Repeat） polymorphic markers from rice, wheat, oat and barley, a total of 226 alleles were found, which exhibited moderate level of diversity. The number of alleles per primer ranged from two to nine, with an average of 4.91. The range of polymorphism information content （PIC） was 0.2844）.980 （average, 0.793）. The expected heterozygosity （He） varied from 0.346 to 0.989, with an average of 0.834. The average coefficient of the genetic similarity of SSR markers among the 118 accessions was 0.609, and it ranged from 0.461 to 0.851. The UPGMA （Unweight Pair Group Method with Arithmetic Mean） clustering analysis at the genetic similarity value of 0.609 grouped the 118 accessions into five groups. Mantel test meant that geographical origin and genetic distance presented positive correlation. The clustering results were consistent with known information on ecological growing areas. The genetic similarity coefficient of the accessions in the Loess Plateau ecotype was significantly lower than those in the other ecotypes. It indicates that the highest level of genetic diversity occurred in the Loess Plateau, which is probably the original site of Panicum miliaceum.
文摘[目的]以6份不同地理来源的糜子资源为试验材料,基于前期转录组测序获得的1000对SSR引物,选出200对进行多态性检测,以期构建一批可以准确评估糜子种质遗传差异的分子标记.[方法]用Primer Premier 5.0软件设计引物,改良CTAB法提取DNA,PCR扩增DNA和聚丙烯酰胺凝胶电泳筛选引物多态性;用PowerMarker 3.25和PopGen 1.32计算遗传多样性参数.[结果]200对引物中97对呈单态性,80对呈多态性.单碱基序列重复引物有20对,10对具多态性,其重复基元是A(50%)和T(50%).二碱基序列重复引物有36对,15对具多态性,其碱基重复类型有7种(AG最多,TC、GC和GA次之,CA、TA和AC最少).三碱基序列重复引物有144对,55对具多态性,其碱基重复类型有24种(GGC、GCG和GCC最多,GAA、GCT和CGC等次之,ACC、AGG、CAG、CGT、AAG、AAC、TCG、CGA、ATT、CAA和CCA最少).就引物分辨率(Rp)值而言,1-3碱基序列重复引物分别为0.67-4.67(平均2.07)、1.33-4.33(平均2.73)和0.67-4.00(平均1.83).基于Rp值分析SSR分布频次,发现80个标记分布在5个区间:0-1、1-2、2-3、3-4和4-5,分别包含17(21.25%)、36(45.00%)、11(13.75%)、14(17.50%)和2个(2.50%).就Rp值而言,1-3碱基序列重复引物分别为0.33-0.67(平均0.51)、0.40-0.78(平均0.59)和0.33-0.83(平均0.59).单碱基序列重复标记共检测到22个等位变异,每个位点为2-3个(平均2.2000),其中8个和2个位点分别具2和3个变异;二碱基序列重复标记共检测到38个等位变异,每个位点为2-3个(平均2.5333),其中7个和8个位点分别具2和3个变异;三碱基重复标记共检测到136个等位变异,每个位点为2-3个(平均2.4727),其中29个和26个位点分别具2和3个变异.就多态性信息含量而言,1-3碱基序列重复引物分别为0.3750-0.5355(平均0.4293)、0.2392-0.7438(平均0.4293)和0.2392-0.7438(平均0.3989).就多样性指数而言,1-3碱基序列重复分别为0.6365-1.0776(平均0.7497)、0.5623-1.0986(平均0.8339)和0.5623-1.0889(平均0.8312).[结论]基于转录组测序结果,检测200对SSR引物的多态性,发现177对(88.5%)可以扩增出完整条带,其中80对具多态性,多态率为40%.
